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Brazilian Journal of Medical and Biological Research

Print version ISSN 0100-879XOn-line version ISSN 1414-431X

Braz J Med Biol Res vol.34 n.1 Ribeirão Preto Jan. 2001 

Braz J Med Biol Res, January 2001, Volume 34(1) 137-141 (Short Communication)

A moderate decrease in temperature inhibits the calcium signaling mechanism(s) of the regulatory volume decrease in chick embryo cardiomyocytes

M.M. Souza and R.T. Boyle

Department of Cell Biology, Duke University Medical Center, Durham, NC, USA

Correspondence and Footnotes


Chick cardiomyocytes, when submitted to hyposmotic swelling, exhibit a partial regulatory volume decrease (RVD). A Ca2+ influx by stretch-activated channels signals a taurine efflux and the RVD at 37°C. We evaluated the cell's performance at room temperature. Cardiomyocytes isolated and cultured from 11-day-old chick embryos were submitted to a hyposmotic solution (180 mOsm/kg H2O) at 37°C and at room temperature (26°C). Under these conditions we measured the changes in cell volume as well as the intracellular free Ca2+ (using fura-2). During hyposmotic swelling, cells at 37°C displayed a peak relative volume of 1.61 ± 0.03 and recovery to 1.22 ± 0.04 (N = 14), while cells at 26°C presented a peak swell relative volume of 1.74 ± 0.06 and did not recover (1.59 ± 0.09, N = 9). Transient increases in intracellular Ca2+, which are characteristic of the normal RVD, were observed at both temperatures (29.1 ± 4.5% (N = 8) and 115.2 ± 42.8% (N = 5) increase at 37° and 26°C (P<0.05), respectively). A delay in the Ca2+ transient increase was also observed when the cells were at 26°C (109 ± 34 s compared to 38 ± 9 s at 37°C, P<0.05). At room temperature the RVD does not occur because the calcium transient increase, which is an early event in the signaling of the RVD, is delayed. Also, free calcium is not cleared as in the 37°C RVD. In the normal RVD the free calcium returns to baseline levels. The very high and persistent free calcium levels seen at room temperature can lead to unregulated enzyme activities and may promote irreversible injury and cell death.

Key words: cell volume, RVD, room temperature, high free calcium

Volume regulatory mechanisms play a critical role in the maintenance of structural integrity, and thus in the proper function of living cells. These regulatory processes involve signal transduction pathways and cellular transport systems.

Cultured chick cardiomyocytes, when submitted to hyposmotic swelling (from 290 to 180 mOsm/kg H2O) exhibit a partial regulatory volume decrease (RVD) mediated by a loss of amino acids (mostly taurine). There are no significant changes in inorganic ion contents in this condition (1). This RVD is dependent on the influx of Ca2+ by stretch-activated channels which in turn signals a taurine efflux and the RVD at 37oC (2-5).

Studies with chick cardiomyocytes under isosmotic conditions at low temperatures reveal a dissipation of the Na+ gradient and low levels of Na+/K+ ATPase activity, with the Na+/Ca2+ exchanger functioning only minimally (6). On the basis of these considerations, we evaluated the RVD under hyposmotic swelling at low temperature.

Cardiomyocytes isolated and cultured from 11-day-old chick embryos (7) were transferred from isosmotic (290 mOsm/kg H2O) to a hyposmotic solution (180 mOsm/kg H2O) at 37oC and at room temperature (26oC). The cells were perfused at a rate of ~4 ml/min with HEPES-buffered saline solution (142.2 mM NaCl, 5.4 mM KCl, 1.0 mM NaH2PO4, 10 mM HEPES, 5.6 mM dextrose, 0.8 mM MgSO4, and 1 mM CaCl2; the hyposmotic solution contained 50% of isosmotic NaCl) on the heated (or not) stage of an inverted microscope (Nikon Diaphot, TMD, Tokyo, Japan). Measurements of cell area from video images of spherical cells (model KP MIL camera, Hitashi Denshi, Ltd.; model PVM-137 monitor, SONY, 1000X resolution) were made using JAVA system software (Jandel Scientific, San Rafael, CA, USA) and were then converted into cell volume. Values were normalized to the last measurement made in the isosmotic solution before volume challenge.

Cells microinjected with fura-2 (1 mM in 140 mM KCl, 10 mM MOPS-3-[N-morpholino] propane-sulfonic acid and passed through a chelex-100 column, pH 7.4) were used for intracellular free Ca2+ measurements by the ratiometric method. The excitation and emission wavelengths were 350/380 and 505 nm, respectively. The coverslips were affixed to a heated (or not) stage on a Zeiss IM35 inverted epifluorescence microscope coupled to a Spex model CM3 dual-wavelength excitation spectrofluorometer (Spex Industries, Edison, NJ, USA).

The mean and standard errors were calculated from relative values and plotted on graphs. Data for control and experimental groups were compared by the Student t-test.

Single cells, when submitted to a change in osmolality (from 290 to 180 mOsm/kg H2O) at 37oC, exhibited a peak swelling of 1.61 ± 0.03 (N = 14) within 2 min, followed by a partial RVD with an end point at 1.22 ± 0.04 (N = 14) (Figure 1). However, when the cells were exposed to the same hyposmotic condition, but at 26oC, the peak of swelling was higher than at 37oC (1.74 ± 0.06, N = 9; P<0.05) and the relative volume was not recovered (1.59 ± 0.09, N = 9; P>0.05) (Figure 1). Also, upon return to isosmotic saline at 30 min, the cells at room temperature returned to pre-swollen levels (relative volume of 1.0), thus further substantiating that no net loss of osmolytes and no RVD results from hyposmotic swelling at 26oC.

When chick cardiac myocytes are submitted to hyposmotic swelling they exhibit a transient increase in intracellular free Ca2+ (2,3), which was also demonstrated in our experiments. The intracellular Ca2+ transient increase (as measured by 350/380) was observed at both temperatures (29.1 ± 4.5%, N = 8, with a delay of 38 ± 9 s at 37oC) (Figure 2). While at 26oC the cells exhibited a 350/380 fluorescence ratio increase of 115.2 ± 42.8% (N = 5) and a delay of 109 ± 34 s (P<0.05, when both parameters were compared with the control temperature) (Figure 2). Post-experiment calculations were performed to calibrate and transform ratio values to Ca2+ concentrations. Values for the different conditions were approximately: control (0.16 µM), hyposmotic solution at 37oC (0.5 µM) and at 26oC (1.3 µM).

Figure 1 - Cultured chick embryo cardiomyocytes cannot activate volume regulatory processes at room temperature. The cells were exposed to a 180 mOsm/kg H2O hyposmotic stimulus (hypo) at 37oC (circles) and 26oC (squares). At 26oC the myocytes cannot recover to near control volumes as compared to the myocytes at 37oC. Data are reported as means ± SEM, N = 14 for 37oC and N = 9 for 26oC. Vo: Observed volume; Vi: initial volume.

[View larger version of this image (31 K GIF file)]

Figure 2 - Intracellular free Ca2+ measurements expressed as fluorescence ratio. Representative experiments showing Ca2+ transient increases in hyposmotic solutions (hypo) at 37oC (A) and 26oC (B).

[View larger version of this image (9 K GIF file)]

In order for cells to maintain a constant volume when subjected to anisosmotic environments, they must have the ability to mobilize osmolytes. The RVD response to hyposmotic swelling is usually characterized by a loss of K+, Cl- and some organic osmolytes (8). In chick cardiac myocytes exposed to a hyposmotic solution, there is a concurrent influx of Ca2+ (2,3) by stretch-activated channels (5), and it has been proposed that protein kinase C, activated by free Ca2+, mediates the amino acid efflux (taurine) that leads to RVD (9). Moreover, it has been shown that cytoskeletal components are involved in the volume regulation of cardiac myocytes. Electrophysiological studies and immunolabeling assays of cytoskeletal proteins reveal suppression of a swelling current and disruption of subplasmalemmal F-actin dynamics induced by cytochalasin B and phalloidin, and the capacity of volume regulation is significantly affected (10,11).

Tissues of mammals exposed to low temperatures show alterations in intracellular ion contents (6,12). For homeotherms, hypothermia can attenuate enzymatic reactions leading to impairment of cell integrity. Considering the differences in temperature dependence for the active and passive transport components of the cell's pump-leak system, which maintains a stable intracellular ion concentration, a change in temperature represents a significant challenge to cellular functions.

Embryonic chick cardiomyocytes in isosmotic solution at low temperatures exhibit an increase in intracellular Na+ concentration. It has been suggested that Na+ influx from Na+/H+ occurs and may not be corrected by Na+/K+-ATPase, which in this situation shows only 15% of its original activity (6). No change was observed in intracellular K+ concentration, and total intracellular Ca2+ presented a small increase at low temperature (6), while intracellular free Ca2+ demonstrated a substantial increase (13).

Few reports regarding cell volume regulation at low temperature are available. Malphigian tubules of a New Zealand mountain insect exposed to hyperosmotic conditions at low temperature (0oC) showed a greater change in cell volume as compared to higher temperatures (20oC) and no regulatory volume increase was observed (14). Some data about chick cardiac myocytes at low temperatures are available (10oC), but only under isosmotic conditions, and in this situation the cells shrink (6). The authors believe that the shrinkage is a consequence of inhibition of the inwardly directed Na+/K+/2Cl- cotransporter.

Our findings reveal that embryonic chick cardiac myocytes during hyposmotic challenge at 26oC (room temperature) swell more than at 37oC, and that at room temperature no RVD is observed. Also, in this situation the intracellular free Ca2+ exhibits a huge increase, much more than a transient increase during hyposmotic swelling at 37oC.

As mentioned previously, during a hyposmotic challenge there is a Ca2+ influx for signaling the RVD which occurs via stretch-activated channels, and the rapid free Ca2+ transient increase at 37oC is a result of Ca2+ influx activating Ca2+ release from intracellular stores (15). At room temperature the transient is also present, but is delayed. Our findings suggest that the Ca2+ release mediated by receptor activities is inefficient at the lower temperature and the same lack of responsiveness may exist with calmodulin, which normally is instrumental in clearing high cytoplasmic free calcium levels through its stimulatory effect on plasma membrane Ca2+ ATPase (16). It is unclear at this point if low temperatures prevent the conformational changes in calmodulin which are necessary to activate Ca2+ ATPase, or if the activated ATPase is less efficient in hydrolyzing ATP at the decreased temperatures. However, the latter is most likely the case and may explain why Ca2+ ATPases in the sarcoplasmic reticulum, which are normally highly efficient in transporting calcium out of the cytoplasm, appear to be inactive at 26oC. In addition, mitochondrial uptake of Ca2+ through its low-affinity/high-capacity Ca2+ pump appears to be temperature sensitive in our model as this mechanism is known to operate when intracellular free calcium becomes perilously high (17). Also, even with abundant free Ca2+, signal transduction pathways that lead to the activation of protein kinase C (and thus to the RVD) are also interrupted.

Taken together, the evidence obtained in this study as well as in other investigations on the chick embryo cardiomyocyte suggests that even moderate hypothermia is a potent inhibitor of active transport. This is manifest in the cardiomyocyte inability to mobilize osmolytes for the RVD response and to control and reduce cytoplasmic calcium concentrations to normal levels. It is well known that if the intracellular free Ca2+ concentration rises to high levels, it levies toxic effects on the cells. The very high and persistent free calcium seen at room temperature can lead to unregulated enzyme activities and promote irreversible cell injury and perhaps cell death.


1. Rasmusson RL, Davis DG & Lieberman M (1993). Amino acid loss during volume regulatory decrease in cultured chick heart cells. American Journal of Physiology, 264 (Cell Physiology, 33): C136-C145.        [ Links ]

2. Smith TW, Rasmusson RL, Freudenrich CC & Lieberman M (1992). Role of Ca2+ in myocardial volume regulation. Circulation, 86: I-480 (Abstract).        [ Links ]

3. Aloi L, Smith JJ, Moore ES, Swaminathan M & Lieberman M (1995). Cardiac cell swelling activates a calcium-dependent signal transduction mechanism. FASEB Journal, 9: A355 (Abstract).        [ Links ]

4. Hall SK, Zhang J & Lieberman M (1997). An early transient current is associated with hyposmotic swelling and volume regulation in cardiac cells. Experimental Physiology, 82: 43-54.        [ Links ]

5. Souza MM, Boyle RT & Lieberman M (2000). Different physiological mechanisms control isovolumetric regulation and regulatory volume decrease in chick embryo cardiomyocytes. Cell Biology International, 24 (in press).        [ Links ]

6. Knerr SMM & Lieberman M (1993). Ion transport during hypothermia in cultured heart cells: Implications for protection of the immature myocardium. Journal of Molecular and Cellular Cardiology, 25: 277-288.        [ Links ]

7. Jacob R, Lieberman M & Liu S (1987). Effects of sodium-potassium pump inhibition and low sodium on membrane potential in cultured embryonic chick heart cells. Journal of Physiology, 387: 549-566.        [ Links ]

8. Hoffmann EK & Dunham PB (1995). Membrane mechanisms and intracellular signaling in cell volume regulation. International Review of Cytology, 161: 172-262.        [ Links ]

9. Smith JJ, Spizz G, Aloi L, Moore ES, Dorbandt A, Blackshear PJ & Lieberman M (1996). Swelling-activated pkC phosphorylation regulates [3H]taurine efflux and RVD in chick cardiac myocytes. FASEB Journal, 10: A314 (Abstract).        [ Links ]

10. Zhang J, Larsen TH & Lieberman M (1997). F-actin modulates swelling-activated chloride current in cultured chick cardiac myocytes. American Journal of Physiology, 273: C1215-C1224.        [ Links ]

11. Larsen TH, Dalen H, Boyle RT, Souza MM & Lieberman M (2000). Cytoskeletal involvement during hyposmotic swelling and volume regulation in cultured chick cardiac myocytes. Histochemistry and Cell Biology, 113: 479-488.        [ Links ]

12. Sudo J & Morel J (1984). Na+ and K+ cell concentration in collagenase treated rat kidney tubules incubated at various temperatures. American Journal of Physiology, 246: C407-C414.        [ Links ]

13. Liu B, Wang LCH & Belke D (1991). Effect of low temperature on the cytosolic free calcium in rat ventricule myocytes. Cell Calcium, 12: 11-18.        [ Links ]

14. Neufeld DS & Leader JP (1998). Cold inhibition of cell volume regulation during the freezing of insect Malphigian tubules. Journal of Experimental Biology, 201: 2195-2204.        [ Links ]

15. Souza MM, Boyle RT & Lieberman M (2000). Comparisons of different stages of embryonic development by the physiological regulatory response to hyposmotic challenge. Comparative Biochemistry and Physiology, 125A: 451-458.        [ Links ]

16. Head JF (1992). A better grip on calmodulin. Current Biology, 2: 609-611.        [ Links ]

17. Carafoli E (1987). Intracellular calcium homeostasis. Annual Review of Biochemistry, 56: 395-433.        [ Links ]

Correspondence and Footnotes

Address for correspondence: M.M. Souza, Departamento de Ciências Fisiológicas, CCB, UEL, Caixa Postal 6001, 86051-990 Londrina, PR, Brasil. Fax: +55-43-371-4207. E-mail:

Presented at the XV Annual Meeting of the Federação de Sociedades de Biologia Experimental, Caxambu, MG, Brazil, August 23-26, 2000. Received April 13, 2000. Accepted September 13, 2000.

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