On-line version ISSN 1414-431X
Braz J Med Biol Res vol.35 no.8 Ribeirão Preto Aug. 2002
Braz J Med Biol Res, August 2002, Volume 35(8) 985-990
M. Mikulecky1 and H.R.K. Lisboa2
1Institute of Preventive and Clinical Medicine, Bratislava, Slovakia
2Departamento de Medicina Interna, Faculdade de Medicina, Universidade de Passo Fundo, Passo Fundo, RS, Brasil
Subjects and Methods
Correspondence and Footnotes
Between October 6, 1997 and April 30, 1999, 5011 births (mean: 8.76 per day) were registered in the city of Passo Fundo, South Brazil. The sequence of 572 daily birth numbers was not random (iteration test). Neyman distribution (m = ¥) showed the best fit. Clusters of days with higher birth numbers alternated with days with low numbers of births. Periodogram analysis revealed a significant periodicity of 6.98 days. The cosinor regression, testing 10 a priori supposed period lengths, found significant seasonality peaking in August-September and significantly highest birth numbers on Thursdays. Among the lunar and solar rotation cycles, the tropic lunar cycle and its 4th harmonic were most pronounced, in agreement with results concerning natality in Germany obtained by Svante Arrhenius in the 19th century. These findings confirm Derer-Halberg's concept of multiseptans. In addition to cycling, a significantly increasing linear trend with a daily increase of 0.0045 births was encountered. This documents a growth of the population in agreement with national statistical data.
Key words: Births, South Brazil, Periodogram, Cosinor, Seasonality, Weekly rhythm, Multiseptans, Cosmogeophysical-biomedical parallelism, Solar and lunar cycles
Cosmogeophysical changes undoubtedly influence many events occurring on earth. The seasons are the most straightforward example that the position of earth in relation to the sun induces changes in temperature and other parameters on our planet. The same is true for sea tides occurring according to the movement and position of the sun and the moon. Besides gravitational forces, there is a daily regular magnetic field variation arising from current systems caused by regular solar radiation changes and affecting the magnetosphere and the ionosphere. Changes in geomagnetic activity may act on living subjects (1).
The influence of cosmogeophysical factors on living organisms has long been studied, sometimes with contradicting results. For example, pronounced and persistent seasonal patterns in fertility have been observed in virtually all human populations. This seasonality increased in the 20th century in some high income, low fertility populations such as that of Sweden (2). Temperature did not play any role in explaining the pronounced June-July peak in conceptions in this country. Strong evidence, however, has been found that summer heat plays an important role in explaining the July-August trough in conceptions in the southern United States. For some populations, obviously factors other than temperature play an important role in birth seasonality.
There are conflicting reports about randomness of daily sequence of birth numbers in various geographic areas. A fluctuation along synodic lunar phases was and continues to be searched for with considerably contradictory results. The objective of this study was to address the question of random sequence. In the case of non-randomness, manifested as a clustering in time in the form of "miniepidemics", the possible presence of periodicities and parallelisms with some known cosmogeophysical cycles, including the synodic lunar one, will be tested besides the general trend, using appropriate chronobiometric tools.
The daily numbers of spontaneous births in the city of Passo Fundo, RS, Brazil, from October 6, 1997 to April 30, 1999 were registered1. The data were obtained on 572 consecutive days for three hospitals that have maternity units in the city, where 99% of deliveries take place. The number of births was taken from the register book of these hospitals after confirmation by the nurse in charge. Births eligible for study were those occurring by the vaginal route and by cesarean section due to obstetric indication (cephalic-pelvic disproportion, hypertensive disease of pregnancy) or due to fetal distress, and those in which the mother asked for surgical delivery but was already considered to be in the beginning of the normal labor (having more than three contractions in 10 min). All these cases were included to insure the spontaneous character of deliveries. Elective cesarean births, considered to occur during working hours (7:30 am to 6:00 pm), were not included in the study.
As descriptive statistics, the mean and 95% confidence interval were calculated for the given daily numbers (counts) of births using a formula based on a normal approximation of the Poisson distribution (3).
To test the randomness of the sequence of daily numbers of births the iteration test was used (4). The null hypothesis was tested against the one-sided alternative supposing a clustering effect. Moreover, the data were analyzed on the basis of null hypotheses supposing normal, Poisson, negative binomial and Neyman distributions type A, B and those with parameters m = 8 and m = ¥ (5).
Periodogram analysis (6,7) was subsequently used to test the presence of any significant periodicity.
The significance of a priori supposed periods (year, half year, synodic, anomalistic and tropical lunar cycles with their 4th harmonics, Bartels solar rotation cycle and social week with a precise 7.00-day period length) was tested by Halberg's cosinor regression (8,9). Also a general linear trend was investigated. The data for cosinor analysis were transformed to moving averages from three successive observations each. The level of statistical significance was set at a = 0.05. For the cosinor calculations, testing the presence of 10 period lengths together, the Bonferroni modification (10) of the P value was used to cope with the problem of spurious significance.
The daily number of births ranged from 0 to 20, with a mean of 8.76 births per day (95% confidence interval 8.52-9.01) and a median of 8.5 births per day.
The one-sided iteration test rejected the randomness of sequence of daily birth numbers in favor of the clustering alternative (P = 0.047). In other words, there are days with significant accumulation of births - something like mini-assemblages - and those with lower numbers of deliveries. This was confirmed and made more precise by distribution testing (Table 1). The best fitting distribution appears to be - due to the highest P value (0.306>0.05) - the Neyman type m = ¥ (Figure 1).
The result of the periodogram analysis was unequivocal (Figure 2, top). One range of significant period lengths was found, i.e., 6.95-6.98 days with P values of 0.039-0.029.
Cosinor analysis disclosed (Figure 2) a significant linear increasing trend with a regression coefficient estimate of 0.0045 cases per day (95% confidence interval 0.0035-0.0054, P<0.001). Table 2 gives the amplitudes as the point and 95% confidence estimates of percentage from the mesor (the starting zero position of the oscillation) and their significance. The time of the first peak for each of the ten tested period lengths is also given. There have been 30% of the variance explained by the cosinor regression. This estimate of the coefficient of determination (0.30) corresponds to the estimate for the coefficient of nonlinear "correlation" (as the square root of 0.30) equal to 0.55. Consequently, the significance of the latter for N = 572, i.e., for 572-12 (12 = number of estimated parameters) = 560 degrees of freedom, P<0.001, speaks in favor of an excellent approximation of the data by the total approximating function.
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The novelty of this study is four-fold. First, the randomness of the sequence of daily numbers of deliveries was tested as the first step of the investigation using the iteration test as well as the Poisson and Neyman distributions. Only rejection of this null hypothesis justified the second step - a search for periodicities and subsequent hypothesizing concerning putatively linked cosmogeophysical variables.
Second, not only a parallelism with synodic "lunar phases", commonly investigated and discussed, but also a parallelism with other cosmogeophysical cycles was taken into account.
Third, appropriate statistical techniques were used. Instead of the usually constructed plexogram in which all data are condensed into one ideal cycle, e.g., from the new moon over the full moon to the next new moon, etc., the chronogram was analyzed. The latter represents the original sequence of data, in our case from October 6, 1997 to April 30, 1999. This makes it possible to test the presence of several cycles together, taking into account in our case three kinds of moon cycles, solar rotation cycle, social week and year seasons. This agrees with the reality of coexistence of many cycles in biomedical time data.
Fourth, as the outcome of these approaches, some interesting results were obtained, particularly the seasonality of births at a given location in the south hemisphere peaking in August and September, the weekly rhythm with the peak on Thursday and perhaps period lengths parallel to some lunar and solar cycles.
Our findings about the synodic lunar cycle should be compared with relatively numerous, often controversial reports in the literature. Guillon et al. (11,12), analyzing millions of births in France, found peaks in May (northern hemisphere!), on Tuesday and a few days before the new moon. Agreement with our findings was only partial. On the other hand, Ghiandoni et al. (13-15) in Italy, with 1248 births, claimed that the maximum number of births was around the full moon - in agreement with our finding. A similar experience was interpreted as a phenomenon connected to the fluctuation of barometric pressure (16). This concept, however, was not confirmed (17).
The most pronounced "cosmic" parallelism in our data appears to be that connected to the tropical lunar cycle and its 4th harmonic. This confirms the results of Arrhenius (18) published one century ago and commented by one of us (19). Even the phase of the tropical lunar cycle with peaking numbers of births seems to agree roughly.
Equally numerous investigators, however, were unable to find any lunar "dependence" of daily birth frequencies (20-24). A detailed historical survey of negative results with a critical approach to positive ones was provided by Wunder (25,26).
The controversial debate concerning the "moon and births" issue appears to be extant in the nineties (8 papers in our review), the same way as in the eighties (6 papers in our review). Since 1998, only two papers (27,28) appeared in Science Citation Index/Current Contents (SCI/CC) journals. Both, however, were dealing with the topic which touches astrology - a feature which is not present in our paper. Nevertheless, they appeared in journals covered by the SCI/CC system!
In the literature, the issue of a time parallelism between some medical events and the synodic lunar cycle is discussed also for some topics of human pathology, usually with negative conclusions. Nevertheless, a cycling parallel to phases of synodic moon was described by our group for paroxysmal tachyarrhythmia (29) and for acute infectious gastroenteritis (30). The second and 4th harmonic of the synodic lunar cycle - approximately fortnight and week periods - were unequivocally present in these data.
A significant 6.5-day rhythm was described originally by Derer (31) for leukocyte counts after treatment of leukemia. On this basis, a general biological concept of circaseptans - approximately 7-day rhythms and their harmonics (the circasemiseptan, around 3.5 days) as well as their multiples (e.g., circadiseptan, i.e., fortnight) - was created (32,33).
To conclude, the sequence of daily birth numbers in this study was not random: clusters of days with higher birth numbers alternating with those having low numbers of births were detected. Periodogram analysis revealed a significant periodicity of 6.98 days. A significant seasonality peaking in August-September and significantly higher birth numbers on Thursdays were found. Among the lunar and solar cycles, the tropic lunar cycle and its 4th harmonic were the most prominent ones. Besides the cycling, a significantly increasing linear trend with a daily increase of 0.0045 births was encountered and attributed to the growth of the population in Brazil.
Results presented in this paper should stimulate similar studies in Brazil as well as worldwide, including either hemisphere. Possible differences and agreements could expand the body of knowledge in the field of chronobiology of human reproduction.
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We would like to thank Drs. Benhur Madalosso and Sheila Meincke Eikhoss, Medical School of the University of Passo Fundo, for their valuable effort in collecting the data from the hospitals.
Address for correspondence: M. Mikulecky, UPKM, Limbova 14, SK 83301 Bratislava, Slovakia. Fax: +421-02-5477-3906. E-mail: email@example.com
Research supported by the Ministry of Health of the Slovak Republic and the Institute of Preventive and Clinical Medicine, Bratislava, Slovak Republic. Received November 14, 2001. Accepted June 3, 2002.