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Brazilian Journal of Medical and Biological Research

Print version ISSN 0100-879XOn-line version ISSN 1414-431X

Braz J Med Biol Res vol.36 no.1 Ribeirão Preto Jan. 2003

https://doi.org/10.1590/S0100-879X2003000100006 

Braz J Med Biol Res, January 2003, Volume 36(1) 39-44

Slaughterhouse blood as a perfusate for studying myocardial function under ischemic conditions

K. Bendjelid1, E. Canet1,2, Y. Gasche4, X. Andre-Fouet3, D. Revel2 and M. Janier1

1Medical Research Centre with Positron Emission (CERMEP), Lyon, France
2CREATIS UMR 5515 CNRS, and 3Claude Bernard-Lyon I University, Louis Pradel Thoracic and Cardiovascular Hospital, Lyon, France
4Surgical Intensive Care Division, University Hospitals of Geneva, Geneva, Switzerland

Abstract
Introduction
Material and Methods
Results
Discussion
References
Acknowledgments
Correspondence and Footnotes


Abstract

Metabolic studies using the in vitro non-recirculating blood-perfused isolated heart model require large volumes of blood. The present study was designed to determine whether heterologous pig blood collected from a slaughterhouse can be used as perfusate for isolated pig hearts perfused under aerobic and constant reduced flow conditions. Eight isolated working pig hearts perfused for 90 min at a constant flow of 1.5 ml g-1 min-1 with non-recirculated blood diluted with Krebs-Henseleit bicarbonate buffer at a hematocrit of 23% were compared to eight hearts subjected to the same protocol but perfused only with Krebs-Henseleit bicarbonate buffer solution. Hearts were paced at 100 bpm and subjected to aerobic perfusion at 38ºC. Hearts were weighed before perfusion and at the end of the experiment and the results are reported as percent weight gain (mean ± SD). Comparisons between groups were performed by the Student t-test (P<0.05). After 90 min of perfusion with modified Krebs-Henseleit, perfused hearts presented a larger weight gain than blood-perfused hearts (39.34 ± 9.27 vs 23.13 ± 5.42%, P = 0.003). Left ventricular end-diastolic pressure was higher in the modified Krebs-Henseleit-perfused group than in the blood group (2.8 ± 0.4 vs 2.3 ± 0.3 mmHg, respectively, P = 0.01). We conclude that heterologous blood perfusion, by preserving a more physiological myocardial water content, is a better perfusion fluid than modified Krebs-Henseleit solution for quantitative studies of myocardial metabolism and heart function under ischemic conditions.

Key words: Myocardium, Ischemia, Erythrocytes, Edema, Reperfusion injury


Introduction

Isolated perfused heart preparations provide controlled conditions for the study of myocardial function and metabolism. Most studies utilize a hemoglobin-free perfusate, such as Krebs-Henseleit solution, which requires high arterial oxygen tension (1) and a high coronary perfusion rate (2). Although Krebs-Henseleit-perfused isolated heart experimentations have the advantage of low cost, simplicity and avoidance of thrombus formation, these preparations are unphysiological because they are devoid of many of the vital components of blood. On the other hand, recent studies have shown that crystalloid-perfused hearts present significantly more edema than blood-perfused hearts after ischemia and reperfusion (3,4). Furthermore, increased myocardial water content compromises left ventricular function and contributes to ultrastructural damage to the myocardium (5).

Generally, metabolic studies are performed using an in vitro model of non-recirculating heart perfusion which requires a large perfusate volume, preventing blood utilization. In this context, blood obtained from slaughterhouses, available in large quantities, may be an interesting alternative. The present study was designed to quantify and compare the weight gain of working isolated pig hearts perfused with Krebs-Henseleit solution or with blood collected from a slaughterhouse. We have developed a blood-perfused working isolated pig heart preparation adapted from the work of Ferrera et al. (6) in order to investigate the protective effect of a heterologous blood perfusate on the appearance of edema during a prolonged (90 min) perfusion under ischemic conditions.


Material and Methods

This study conforms with the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH Publication # 85-23, revised in 1996).

Perfusate composition

A modified Krebs-Henseleit bicarbonate buffer (7) (pH 7.4; temperature, 38ºC) was prepared to obtain the following final concentrations: 118 mM sodium chloride, 4.7 mM potassium chloride, 2.4 mM calcium chloride, 1.2 mM potassium phosphate, 0.5 mM calcium-EDTA, 11 mM glucose, 25 mM sodium hydrogen carbonate, and 1.2 mM magnesium sulfate. The modified Krebs-Henseleit-perfused group was oxygenated with an oxygenator (Monolyth, Sorin 050162-P7852, Saluggia, Italy) using 95% oxygen plus 5% carbon dioxide to achieve a partial arterial oxygen pressure >400 mmHg.

For each experiment carried out on the group of hearts perfused with blood, 10,000 ml of blood were collected into a container from 4 to 5 pigs at a slaughterhouse (Mornant, France). The pigs were exsanguinated by incision of the carotid artery after paralyzing by an electric current. Next, 5000 U heparin per 1000 ml blood was added to the container. The blood was filtered and stored at 4ºC for a maximum of 1 h and then diluted with the modified Krebs-Henseleit buffer to obtain a hematocrit of 23%. Blood perfusate oxygenation was carried out with the same oxygenator using 95% oxygen plus 5% carbon dioxide to achieve an arterial partial oxygen pressure of 140 mmHg. The perfusion apparatus maintained the coronary perfusate at 38ºC.

Isolated pig heart preparation

In a first step, the preparation was set up according to the methods of Langendorff (8) and Kobayashi and Neely (9) for isolated heart preparations. In a second step, we selectively cannulated the right and left main coronary arteries to precisely control regional myocardial perfusion separately from cardiac function.

Farm pigs (20-25 kg) were fasted overnight before surgery. Fifteen minutes before anesthesia, 50 mg droperidol (Droleptan®) was injected intramuscularly. Intravenous anesthesia was then performed with thiopental (4 mg/kg, Nesdonal®). Mechanical ventilation (Monal; minute ventilation, 4 liters; respiratory rate, 13; 50% oxygen) was provided after rapid tracheotomy using a No. 75 endotracheal tube. Through a sternotomy, the heart was harvested after aortic clamping and infusion of 500 ml cold (4ºC) St. Thomas No. 2 cardioplegia solution (Laboratoires Aguettant, Lyon, France) (10). Next, the heart was weighed, the pulmonary artery cannulated, the inferior and superior vena cava ligated, and the aorta fixed onto the system. The left atrium was maintained open to allow drainage of the Thebesius effluent. A latex balloon was inserted into the left ventricle, inflated with water and fixed on the mitral valve annulus. Proximal left and right coronary arteries were dissected and cannulated selectively. The preparation time (from excision to reperfusion) for the isolated heart preparation ranged from 20 to 25 min. During this preparation, hearts were maintained in cold (4ºC) St. Thomas solution. Next, the aorta was fixed onto the perfusion system and hearts were reperfused at 38ºC.

Experimental protocols

A total of 16 hearts were perfused either with the modified Krebs-Henseleit solution as a perfusate (N = 8) or with the blood perfusate (N = 8) using an electromagnetic Gear pump (MCP-Z, Ismatec, Zurich, Switzerland). A constant flow rate of 1.5 ml g-1 min-1 was applied to each heart group for 90 min. Both perfusion systems consisted of a non-recirculating apparatus and the coronary effluent was collected from the pulmonary artery catheter. Two epicardial electrodes were positioned through the right atrium, and hearts were paced at a constant rate of 100 bpm throughout the entire protocol. The intraventricular latex balloon was filled with a volume of saline to produce an end-diastolic pressure of 5 mmHg. It was then connected to an artificial heart system (Abiomed Inc., Danvers, MA, USA), which permits the adaptation of preload (3 mmHg) and afterload (70 mmHg). The pressure transducer system used was a rigid fluid-filled cannula connected to a pressure transducer (Medex, SX 620 449 CST, Annecy-le-Vieux, France). Myocardial oxygen consumption was calculated as the product of coronary flow and coronary arteriovenous oxygen content difference. At the end of the experiment, hearts and excess fluid were gently removed. The balloon and the pulmonary artery catheter were removed and hearts were weighed.

Statistical analysis

Descriptive statistics such as proportions, means, and standard deviations were used to summarize the results. The paired Student t-test was used to compare heart weights before and after perfusion for each group. The unpaired Student t-test was used to compare heart weight, heart weight gain, left ventricular end-diastolic pressures and left ventricular developed pressures between the two groups. Differences were considered significant when P<0.05.


Results

The substrate composition of the blood coming from the slaughterhouse is shown Table 1. During the experiment, we monitored the oxygen uptake of the blood-perfused and modified Krebs-Henseleit solution-perfused preparations (Table 1). Coronary perfusion pressures of modified Krebs-Henseleit- and blood-perfused hearts increased regularly throughout the experiment. Heart rate (mean ± SD) was stable at 100 ± 2 bpm during this 90-min period. After 90 min of perfusion, left ventricular end-diastolic pressure (mean ± SD) was significantly higher in the modified Krebs-Henseleit-perfused group than in the blood group (2.8 ± 0.4 vs 2.3 ± 0.3 mmHg, respectively, P = 0.01). Left ventricular developed pressure was significantly higher in the blood-perfused group than in the modified Krebs-Henseleit-perfused group (73.3 ± 1.5 vs 70.2 ± 1.4 mmHg, respectively, P = 0.0008). The arteriovenous difference between the pO2 measured in the arterial line and the pO2 measured in the coronary return remained constant throughout the experiment for both perfusates, demonstrating a good extraction, hence a good-quality myocardial oxygenation. The mean ± SD weight of modified Krebs-Henseleit- and blood-perfused hearts was 148.94 ± 20.59 and 143.99 ± 19.27 g, respectively, before perfusion (P = 0.63). For both groups, heart weight increased after perfusion (P<0.0001). After 90 min of perfusion, weight gain was higher in the modified Krebs-Henseleit solution-perfused hearts than in the blood-perfused hearts (39.34 ± 9.27 vs 23.13 ± 5.42%, respectively, P = 0.003; Figure 1).


Figure 1. Heart weight gain after 90 min of perfusion. Hearts were perfused with modified Krebs-Henseleit solution (N = 8) or blood perfusate (N = 8). The horizontal lines indicate the mean. *P<0.05 compared to blood perfusate (Student t-test).

[View larger version of this image (4 K GIF file)]


Discussion

This study compares for the first time the weight gain of working isolated pig hearts perfused with crystalloid solution (modified Krebs-Henseleit) versus heterologous blood obtained from the slaughterhouse. The results indicate that heterologous blood, because of a lower myocardial water accumulation, is more physiological than the Krebs-Henseleit solution after prolonged perfusion under ischemic conditions. Moreover, in parallel to the greater extent of edema in the Krebs-Henseleit group, greater deterioration in the contractile performance was observed, as indicated by the left ventricular pressure measurements. Myocardium was perhaps better preserved from edema by the osmotic and oncotic profile of blood in contrast to modified Krebs-Henseleit perfusion (2). Heterologous blood-perfused isolated hearts have been used in the past (11-13) but, in those studies no comparisons of weight gain or water content was performed. On the other hand, although several studies (3,4,14) support the notion that blood is a superior perfusate to crystalloid solutions particularly when water content is considered, to our knowledge, no study has used heterologous blood from a slaughterhouse.

Metabolic substrates and energetic stability were not analyzed in this study because we were testing the feasibility of the heterologous blood perfusion in this model. Nevertheless, heart rate, myocardial oxygenation, left ventricular end-diastolic pressure, and left ventricular developed pressure were recorded throughout the experiment. All hearts were paced at 100 bpm and perfused at a constant reduced flow to prevent the occurrence of any difference in myocardial edema between the two groups resulting from variations of these parameters (15,16). We used a higher arterial oxygen tension in the modified Krebs-Henseleit solution as a perfusate than in the blood perfusate (421 ± 22 vs 147 ± 9 mmHg) because these myocardial oxygen levels are considered adequate to meet metabolic demands of working isolated hearts according to the type of perfusion (blood versus crystalloid) (1), without influencing myocardial water content (17). Interestingly, the data indicate that the greater edema observed in the Krebs-Henseleit group seemed not to be due to hypoxia since arterial pO2 was adequate, but to other ischemia-associated factors.

In this study, we were able to separate coronary perfusion from contractile function using a latex balloon inserted into the left ventricle and selective cannulation of coronary vessels. Thus, our model should be useful for metabolic studies. In this mode, an accurate quantification of metabolic balance is possible since the same perfusate passes only once through the heart, preventing the accumulation of metabolic substances. However, this system requires large quantities of blood, impossible to collect from a single animal. In this setting, the slaughterhouses are an alternative for obtaining large volumes of blood and, despite the mix of blood from different animals, no hemolysis complications were noted in the present study. This may be explained by the lack of natural antibodies against blood group factors in pigs (18).

Study limitation

A high oxygen tension model normally used for studying myocardial hypoxia was employed. Indeed, myocardial oxygen consumption (136 ± 18 vs 163 ± 17 µl min-1 g-1 for modified Krebs-Henseleit solution and blood, respectively) ensures that the edema produced in the modified Krebs-Henseleit group is not the result of hypoxia induced by the experimental design. However, we subjected all isolated hearts to the same reduced coronary flow, a procedure that probably prevented any difference in myocardial edema between groups resulting from variations of perfusion. Furthermore, compared to other studies (3,4), the present protocol permitted us to determine if the difference in myocardial edema between the modified Krebs-Henseleit group and the heterologous blood group existed when isolated hearts were subjected to ischemic conditions.

In the present study, we measured only weight gain but not water content (3,4). Indeed, direct assessment of edema formation by estimation of changes in myocardial water content is more accurate than estimation of weight gain. Nevertheless, continuous weight gain can be extrapolated to edema formation since excess fluid surrounding the heart was gently removed after perfusion. In addition, in previous studies on isolated rabbit lungs perfused with blood, the combined histologic score of lung hemorrhage was correlated with weight gain (19,20).

Our results demonstrate that weight gain by working isolated pig hearts, a surrogate of myocardial edema formation, increased more after modified Krebs-Henseleit solution perfusion than after heterologous blood perfusion under ischemic conditions. Since the presence of myocardial edema represents one of the crucial events in the pathophysiology of cardiac dysfunction following reperfusion injury, heterologous blood perfusate obtained from a slaughterhouse should be used as an alternative to modified Krebs-Henseleit solution as a perfusate for quantitative experimental studies of myocardial metabolism and heart function. Moreover, an essential goal of using blood as a perfusate instead of crystalloids is to mimic the in vivo situation. Indeed, including the interaction between endothelial cells and blood elements such as erythrocytes, platelets and leukocytes should be attractive to reduce heart reperfusion injury in working heart preparations.


References

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Acknowledgments

The authors gratefully acknowledge Cendrine Casali and Annie Desenfant for technical assistance. The authors also thank Jean Francois Obadia and René Ferrera for helpful advice regarding the preparation, and the Abattoir of Pavière (Mornant, France) for providing the blood.


Correspondence and Footnotes

Address for correspondence: K. Bendjelid, Chef de Clinique Division des Soins Intensifs Chirurgicaux, Hôpitaux Universitaires de Genève, CH-1211 Genève 14, Switzerland. Fax: +41-22-382-7455. E-mail: karim.bendjelid@hcuge.ch

Some of these data have been presented at the III Congress of GRRC (Groupe de Réflexion sur la Recherche Cardio-vasculaire), Montpellier, France, April 26-27, 2001. Arch. Mal. Coeur., 94: 380, 2001 (No. 27-5). Received May 10, 2002. Accepted October 14, 2002.

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