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Association of apolipoprotein E polymorphism in late-onset Alzheimer's disease and vascular dementia in Brazilians

Abstract

The genetic basis for dementias is complex. A common polymorphism in the apolipoprotein E (APOE) gene is considered to be the major risk factor in families with sporadic and late-onset Alzheimer's disease as well as in the general population. The distribution of alleles and genotypes of the APOE gene in late-onset Alzheimer's disease (N = 68), other late-life dementias (N = 39), and in cognitively normal controls (N = 58) was determined, as also was the risk for Alzheimer's disease associated with the epsilon4 allele. Peripheral blood samples were obtained from a total of 165 individuals living in Brazil aged 65-82 years. Genomic DNA was amplified by the polymerase chain reaction and the products were digested with HhaI restriction enzyme. APOE epsilon2 frequency was considerably lower in the Alzheimer's disease group (1%), and the epsilon3 allele and epsilon3/epsilon3 genotype frequencies were higher in the controls (84 and 72%, respectively) as were the epsilon4 allele and epsilon3/epsilon4 genotype frequencies in Alzheimer's disease (25 and 41%, respectively). The higher frequency of the epsilon4 allele in Alzheimer's disease confirmed its role as a risk factor, while epsilon2 provided a weak protection against development of the disease. However, in view of the unexpectedly low frequency of the epsilon4 allele, additional analyses in a more varied Brazilian sample are needed to clarify the real contribution of apolipoprotein E to the development of Alzheimer's disease in this population.

Alzheimer's disease; Vascular dementia; Dementia; Apolipoprotein E; Aging; Genetic polymorphisms


Braz J Med Biol Res, July 2003, Volume 36(7) 919-923 (Short Communication)

Association of apolipoprotein E polymorphism in late-onset Alzheimer's disease and vascular dementia in Brazilians

D.R.S. Souza1, M.R. De Godoy2, J. Hotta3, E.H. Tajara4, A.C. Brandão1, S. Pinheiro Júnior1, W.A. Tognola2 and J.E. Dos Santos3

Departamentos de 1Biologia Molecular, and 2Ciências Neurológicas, Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, SP, Brasil

3Departamento de Clínica Médica, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil

4Departamento de Biologia, Universidade Estadual Paulista Júlio de Mesquita Filho, São José do Rio Preto, SP, Brasil

Text

References

Correspondence and Footnotes Correspondence and Footnotes Correspondence and Footnotes

Abstract

The genetic basis for dementias is complex. A common polymorphism in the apolipoprotein E (APOE) gene is considered to be the major risk factor in families with sporadic and late-onset Alzheimer's disease as well as in the general population. The distribution of alleles and genotypes of the APOE gene in late-onset Alzheimer's disease (N = 68), other late-life dementias (N = 39), and in cognitively normal controls (N = 58) was determined, as also was the risk for Alzheimer's disease associated with the e4 allele. Peripheral blood samples were obtained from a total of 165 individuals living in Brazil aged 65-82 years. Genomic DNA was amplified by the polymerase chain reaction and the products were digested with HhaI restriction enzyme. APOE e2 frequency was considerably lower in the Alzheimer's disease group (1%), and the e3 allele and e3/e3 genotype frequencies were higher in the controls (84 and 72%, respectively) as were the e4 allele and e3/e4 genotype frequencies in Alzheimer's disease (25 and 41%, respectively). The higher frequency of the e4 allele in Alzheimer's disease confirmed its role as a risk factor, while e2 provided a weak protection against development of the disease. However, in view of the unexpectedly low frequency of the e4 allele, additional analyses in a more varied Brazilian sample are needed to clarify the real contribution of apolipoprotein E to the development of Alzheimer's disease in this population.

Key words: Alzheimer's disease, Vascular dementia, Dementia, Apolipoprotein E, Aging, Genetic polymorphisms

In North America and Europe the most common dementia that affects the elderly is Alzheimer's disease (AD), corresponding to 55.6 to 72% of all dementia cases (1,2). In a community-dwelling Brazilian population the prevalence of AD and vascular dementia (VD) was 55.1 and 9.3%, respectively (3).

The genetic basis for dementias is complex. For familial early-onset AD, there is some evidence for mutations in the ß-amyloid precursor protein (APP, chromosome 21) and presenilin 1 and 2 genes (chromosomes 14 and 1, respectively). Considering the development of sporadic and late-onset AD (LOAD), a common polymorphism in the apolipoprotein E (APOE) gene (chromosome 19) is the major risk factor in families with the disease, as well as in the general population (4). More recently, a new LOAD locus on chromosome 10 independent of the APOE genotype was discovered (5).

There are three common APOE alleles (e2, e3 and e4), accounting for more than 99% of the isoforms of the product of apo E. The e3 allele is the most frequent, representing 74 to 86% of all alleles in European and American Caucasian populations. The e4 allele frequency is approximately 7 to 16% and the e2 allele frequency is 6.3 to 12% (6-10). Of these three, e4 is recognized as a risk factor for LOAD, whereas the e2 and e3 alleles appear to enhance tolerance to the brain degenerative process, extending the survival of affected neurons. The effect of the APOE e4 allele has been associated with the pattern of regional brain atrophy in AD (11). In addition, the combination of low head circumference and e4 predicts early-onset AD (12). Furthermore, the apo E polymorphism in a community sample of middle-aged adults was associated with memory performance (13).

Several studies have confirmed the association of apo E and the accumulation of amyloid ß-protein, a characteristic of AD. The early description of this peptide was reported to be associated with the presence of the e4 allele in autopsy cases even without signs of dementia (14). In addition, individuals with the e4 allele and mutations in the APP gene have earlier-onset disease compared to those with the e2 or e3 allele and mutations in APP (15).

In fact, many case-control and post-mortem studies of LOAD patients have shown e4 allele frequencies ranging from 17 to 57% in both sporadic and familial cases (6-9,16,17). The APOE genotype, also investigated in other types of dementias, has shown a slight association with VD, Pick's disease and Lewy's body disease but no association with Parkinson's disease or Creutzfeldt-Jakob disease (10,18).

The literature, in general, suggests that there is a relationship between the e4 allele and AD. In the present study we analyzed the distribution of APOE alleles and genotypes in Brazilian patients with clinical signs of LOAD or other types of late-life dementias, mainly VD. We also evaluated the risk for AD associated with the e4 allele.

A total of 165 Caucasian individuals aged 65-82 were studied. Cognitively impaired patients were divided into two groups: a LOAD group (39 men and 29 women) and a group with other late-life dementias (23 men and 16 women) including VD (N = 35), Parkinson's disease (N = 3) and hypothyroidism dementia (N = 1). Control subjects (28 men and 30 women) were members of an elderly healthy group attended in a neurogeriatric unit of a teaching hospital (Faculty of Medicine of São José do Rio Preto, São José do Rio Preto, SP, Brazil). The median ages of the participants at the beginning of this study were 71.5, 72.0 and 70.0 years for patients with AD, other dementias and controls, respectively, without significant difference among them (P = 0.14). A trained physician interviewed each subject or person responsible and obtained informed consent and blood samples. The subjects were diagnosed and classified by historical and physical examination as well as by neuropsychological (NINCDS-ADRDA) criteria and laboratory tests according to standard protocols and guidelines. The study was approved by the Hospital Ethics Committee.

Genomic DNA extraction and APOE genotyping were conducted according to standard procedures (19,20). Intragroup frequencies of APOE e2, e3 and e4 alleles were evaluated by adjusting the chi-square test with equiprobability. Allele and genotype frequencies were compared among groups by the test of proportion by normal approximation. The association between the e4 allele and AD and between the e2 allele and a protective effect was evaluated by the odds ratio (OR). The level of significance was set at a = 0.05 for all analyses.

Table 1 shows the distribution of the APOE alleles and genotypes in patients and controls. APOE e2 was less frequent in AD than in controls and the e4 frequency was significantly higher in AD than in controls (P = 0.004) but no significant difference was observed for other dementias versus controls (P = 0.46).

Association of apolipoprotein E polymorphism in late-onset Alzheimer's disease and vascular dementia in Brazilians. D.R.S. Souza, M.R. De Godoy, J. Hotta, E.H. Tajara, A.C. Brandão, S. Pinheiro Júnior, W.A. Tognola and J.E. Dos Santos. Brazilian Journal of Medical and Biological Research, 36 (7): 919, 2003.

 

Population data have shown a wide variation in the frequency of the e4 allele, probably reflecting different methodologies, ethnic diversity, and sample sizes for AD (17 to 57%) versus control groups (7 to 16%) (6-9,16,17). As reported by other investigators, patients also showed a slightly increased frequency of e4 in both VD and Lewy's body disease (10,18).

In the present study, although the e4 frequency was twice as high in AD (0.25) compared to controls (0.12), it was still lower than in many other studies. However, it was consistent with the limited Brazilian data ranging from 0.21 to 0.39 (7-10,16), without a significant difference between patients with presenile or senile dementia (8).

Furthermore, there are the possible confounding effects of age and gender (11). This suggests that each group is a different subset in the general population. Studies of patients with AD and their relatives can permit a reliable expansion of experiments to measure the prevalence of the APOE alleles in familial or sporadic AD, due to the hereditary nature of the apo E polymorphism. Cação J, De Godoy MR, Pinhel MA, Scudeler D, Fernandez MR, Ruiz V, Romero AMM, Tognola W, Hotta J, Dos Santos JE and Souza DRS (2002, personal communication) showed a significant increase of the e4 allele in relatives of AD patients (0.24) compared with relatives of controls in a Brazilian population (0.04).

The e3/e3 genotype frequencies were higher than other apo E genotype frequencies (e2/e2, e2/e3, e2/e4, e3/e4, e4/e4), specifically in controls when compared to AD in this study (Table 1). Contrasting results were observed for the e3/e4 genotype in AD (41%) and in controls (16%; P = 0.0008). This prevalence is in agreement with other Brazilian series only for controls but not for patients, with the values reported in the literature being 27.3 and 35% for Caucasian patients versus 17.9 (P = 0.234) and 16% for Caucasian controls, respectively (8,9), or black controls (27%) (9). On the other hand, the e4/e4 genotype was rare mainly in series from the southeast, with values around 5% (8), including this study, while in a southern Brazilian population this prevalence was 17%, but in a small series (9).

The OR for e4 in AD was >1 (Table 2) with a significant effect, again suggesting its influence on the development of the disease. In addition, the estimated OR for e3/e4 confirms the higher risk for AD, as reported in other populations (18). However, no association was found with other late-life dementias, represented in this study mainly by VD, although a slight increase in frequencies of the e4 allele or e3/e4 genotypes was observed in such cases. As many patients with clinical diagnoses of VD prove to have neuropathologic signs of AD, higher values for e4 are expected in this group (6). Similar results were observed for e2 and e3 in other dementias. Furthermore, the presence of the e3/e4 genotype increased the risk for AD by a factor of 3.6 (95% CI = 1.5-8.7) using e3/e3 homozygotes as the baseline. However, no significant effect of the e3/e4 genotypes was found in other dementias.

Association of apolipoprotein E polymorphism in late-onset Alzheimer's disease and vascular dementia in Brazilians. D.R.S. Souza, M.R. De Godoy, J. Hotta, E.H. Tajara, A.C. Brandão, S. Pinheiro Júnior, W.A. Tognola and J.E. Dos Santos. Brazilian Journal of Medical and Biological Research, 36 (7): 919, 2003.

 

The reduced frequency of genotypes with at least one e2 allele in AD substantiates the role of this allele as a protective element. Very low e2 allele frequencies were observed in case-control studies (0.03 to 0.08) and even lower ones in AD (0.01 to 0.08) (7-10,15-18). However, a nonsignificant OR for e2 in AD was observed in the present study, suggesting that the e2 allele has a very little or no effect in terms of presence or absence of AD, while Bahia et al. (16) observed a significant difference between patients with presenile or senile AD (0.05) and controls (0.15; P = 0.009).

The higher frequency of the e4 allele confirmed its role as a risk factor associated with AD, while the e2 and e3 alleles showed a weak protection, if any, against AD development. Considering the unexpected reduced frequency of the e4 allele and the almost complete lack of studies in the Brazilian population, additional analysis with larger and more varied samples is needed to clarify the real contribution of apo E to the development of AD in this population.

Acknowledgments

The authors wish to thank Marcia R.F. Ferraz, José Antonio Cordeiro, Márcio Colombo, Flávio A.V. Seixas, Marcos R.H. Estécio, Luis Carlos Mattos, Rosa Kawasaki Oyama, Peter James Harris, Livia C. Burdmann, Carlos Alexandre A. Torres, Mariléia Scartezini and David A. Hewitt for technical assistance, discussions and comments.

Address for correspondence: D.R.S. Souza, Departamento de Biologia Molecular, FAMERP, Av. Brigadeiro Faria Lima, 5416, 15090-000 São José do Rio Preto, SP, Brasil. Fax: +55-17-227-5733. E-mail: doroteia@famerp.br

Research supported by FAPESP and CNPq. Received July 3, 2002. Accepted February 28, 2003.

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  • Correspondence and Footnotes

  • Publication Dates

    • Publication in this collection
      26 June 2003
    • Date of issue
      July 2003

    History

    • Accepted
      28 Feb 2003
    • Received
      03 July 2002
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