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Brazilian Journal of Medical and Biological Research

On-line version ISSN 1414-431X

Braz J Med Biol Res vol.36 no.8 Ribeirão Preto Aug. 2003

http://dx.doi.org/10.1590/S0100-879X2003000800001 

Braz J Med Biol Res, August 2003, Volume 36(8) 959-966

Dimensions and dynamics in integrin function

K.M. Yamada1, R. Pankov1 and E. Cukierman1,2

1Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA
2Basic Science Division, Fox Chase Cancer Center, Philadelphia, PA, USA

Abstract
Text
References
Correspondence and Footnotes


Abstract

Integrins play crucial roles in cell adhesion, migration, and signaling by providing transmembrane links between the extracellular matrix and the cytoskeleton. Integrins cluster in macromolecular complexes to generate cell-matrix adhesions such as focal adhesions. In this mini-review, we compare certain integrin-based biological responses and signaling during cell interactions with standard 2D cell culture versus 3D matrices. Besides responding to the composition of the matrix, cells sense and react to physical properties that include three-dimensionality and rigidity. In routine cell culture, fibroblasts and mesenchymal cells appear to use focal adhesions as anchors. They then use intracellular actomyosin contractility and dynamic, directional integrin movements to stretch cell-surface fibronectin and to generate characteristic long fibrils of fibronectin in "fibrillar adhesions". Some cells in culture proceed to produce dense, three-dimensional matrices similar to in vivo matrix, as opposed to the flat, rigid, two-dimensional surfaces habitually used for cell culture. Cells within such more natural 3D matrices form a distinctive class of adhesion termed "3D-matrix adhesions". These 3D adhesions show distinctive morphology and molecular composition. Their formation is heavily dependent on interactions between integrin a5ß1 and fibronectin. Cells adhere much more rapidly to 3D matrices. They also show more rapid morphological changes, migration, and proliferation compared to most 2D matrices or 3D collagen gels. Particularly notable are low levels of tyrosine phosphorylation of focal adhesion kinase and moderate increases in activated mitogen-activated protein kinase. These findings underscore the importance of the dimensionality and dynamics of matrix substrates in cellular responses to the extracellular matrix.

Key words: Extracellular matrix, Cell adhesion, Integrin, Fibronectin, Signal transduction, Migration


Introduction

Integrins are the major vertebrate receptors for extracellular matrix molecules. Besides mediating cell adhesion and migration, they help to regulate critical cellular signaling processes that range from cell growth and apoptosis to specific gene regulation. The field of integrin biology has grown explosively over the past decade, and it now encompasses broad areas of cell biology, structural biology, and medicine that have been the subject of a variety of recent reviews (1-13). In this brief mini-review we will focus on roles of integrins in the formation of a fibrillar extracellular matrix and then on the cellular responses to extracellular matrix from our personal perspective. We will review studies showing that, besides its biochemical composition, important features of the extracellular matrix that govern the cellular responses it elicits include its three-dimensionality and its deformability or rigidity. In-depth reviews of other aspects of integrin-based cell-matrix interactions with many more citations than possible in this mini-review have been published previously (1-10,14-19).

Formation of extracellular matrices - the importance of dynamics

Although the capacity of purified collagen to self-assemble into fibrils in the absence of cells has been a central conceptual paradigm of matrix biochemistry, the way in which collagen molecules produced by living cells actually become organized into extracellular fibrillar matrices in vivo appears to be under cellular control. In fact, both older and very recent studies provide strong evidence that the fibrillar organization of collagens type I and III, at least in the early stages of organization, depends on the preceding organization of another matrix protein - fibronectin (20-22). Since other extracellular molecules also often co-localize with fibronectin fibrils, it is obviously important to understand how cells form fibrils of fibronectin. Much is now known about the biochemistry of fibronectin matrix assembly (7,23). Crucial elements identified to date include the binding of soluble fibronectin molecules by integrins and then exposure of cryptic fibronectin self-assembly sites to promote self-association; the mechanism for exposure of specific cryptic sites is thought to involve tension (24,25), i.e., stretching of fibronectin by intracellular contractility (7,23,26-29).

Integrin and fibronectin dynamics on the cell surface can be tracked by using non-perturbing antibody tracers and directly labeled fibronectin (30). Different patterns of integrin behavior were identified for two types of integrin receptors. The multi-ligand receptor aVß3 remains within focal adhesions. In contrast, the fibronectin receptor a5ß1 translocates out of focal contacts at 6.5 µm/h along fibrillar adhesions (previously termed "extracellular matrix contacts") in parallel to actin microfilament bundles (30). Fibronectin is translocated in a very similar pattern (28,30). Tensin is a primary cytoskeletal component of fibrillar adhesions, and it also moves in a parallel fashion (31). In fact, a dominant-negative inhibitor of tensin blocks integrin translocation, fibrillar adhesion formation, and fibronectin fibrillogenesis without affecting focal adhesions (30). As shown diagrammatically in Figure 1, we proposed that translocating a5ß1 integrins induce initial fibronectin fibrillogenesis by transmitting cytoskeleton-generated tension to extracellular fibronectin molecules. In fact, blocking this integrin translocation by a variety of treatments prevents both the formation of fibrillar adhesions and fibronectin fibrillogenesis. Thus, fibronectin fibrils appear to be generated by a process of localized, directional, integrin translocation, which exposes cryptic domains on the fibronectin molecule, allowing polymerization into fibers and resulting in matrix assembly.


Figure 1. Integrin translocation mechanism for fibronectin matrix assembly. Cells use focal adhesions as anchors, e.g., for mediating strong adhesion to vitronectin using the aVß3 integrin (A). Intracellular contractility and dynamic, directional movement of the a5ß1 integrin (blue) along actin microfilament bundles from the cell periphery towards the cell center results in a pulling and stretching of fibronectin. The tension from stretching is thought to cause local unfolding of the fibronectin molecule, which exposes cryptic binding sites for assembling a linear fibronectin fibril (B). This process requires the cytoskeletal protein tensin and intracellular actomyosin contractility.

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Cellular responses to extracellular matrices - the importance of dimensions

Once the extracellular matrix is formed, it can regulate numerous aspects of cell behavior (32). A variety of studies of cells cultured in three-dimensional collagen gels and reconstituted basement membrane matrix have underscored the importance of considering cells in more physiological microenvironments than growth on flat tissue culture substrates (32-39). For example, fibroblasts in collagen gels under different degrees of tension behave quite differently (40-43), and epithelial cells in three-dimensional environments show major differences in biosynthesis of differentiated products (44) or in responses to apoptotic stimuli (45,46).

Although a variety of systems have been developed to mimic physiological three-dimensional environments (36,37), a recent approach involves using tissue- or cell culture-derived three-dimensional matrices for analyzing the behavior of migratory and mesenchymal cells (47,48). The logic behind this approach is that cells do not normally encounter a pure collagen matrix, nor a multi-millimeter-thick basement membrane. Instead, they exist in complex environments comprised of multiple molecules and a substantial degree of fibrillar organization with complexity beyond that provided by collagen gels. Recent findings indicate that cells respond differently to such complex 3D matrices compared to collagen gels or flat tissue culture substrates.

Cryostat sections of tissues of embryos from which cellular contents have been extracted with alkaline detergent solution provide a well-organized fibrillar extracellular matrix that can be used as a system for culturing fibroblastic cells in three dimensions. It can be mimicked effectively by extracting morphologically similar dense extracellular matrices accumulated by certain cultured fibroblasts after multiple days of cell culture. In both cases, a key component of these matrices is fibronectin, along with collagen I and heparan sulfate proteoglycan (Cukierman E, unpublished results). Cells plated into such matrices show greater than 6-fold rates of cell attachment compared to purified collagen gels, as well as compared to substrates of purified fibronectin, laminin, or collagen I (47). As initially established for fibroblasts and other cells plated in collagen gels (32,33,49), they acquire an in vivo-like fibroblastic shape that is slender and elongated, rather than flattened and triangular (Figure 2). However, the rate at which human fibroblasts acquire such morphology is much faster in the natural 3D matrices compared to collagen gels; the final morphology is also slightly different. Interestingly, even rates of cell proliferation appear to be augmented more than 2-fold above proliferation rates on the excellent cell culture substrate fibronectin. Finally, cell migration is augmented on such matrices compared to fibronectin, collagen, or 3D collagen gels (47).

Are the effects of complex matrices due to the individual molecular components or to the overall 3D organization? The answer seems to be a combination: flattening the 3D matrices by mechanical force (using a Teflon-covered lead weight) or by coating culture substrates with all of the same molecules using a mixture of 3D matrix components solubilized by guanidine (followed by dialysis) (48) could not mimic any of the enhancing effects of 3D matrix on attachment, spreading, or proliferation. Conversely, not only does providing a collagenous three-dimensional matrix not suffice, but even a fibrillar matrix formed from purified fibronectin does not, implying that some molecular component in the 3D matrix besides fibronectin is needed (47).

The specific type of integrin used by cells for interactions with an extracellular matrix seems to be matrix-specific. In collagen gels, the predominant functional integrin is a2ß1 (50). In contrast, in each of the dramatic effects observed by 3D matrices on cell attachment, morphology, migration, and proliferation, the dominant integrin involved is the fibronectin receptor a5ß1 integrin (47). Another fibronectin receptor, the aVß3 integrin, is also important for cell interactions with purified fibronectin itself (requiring antibody inhibition by both anti-aV and a5 integrins), yet 65-88% of the cellular responses of human fibroblasts to 3D matrices can be suppressed by antibody against a5 while only 27-36% inhibition is observed by the same antibody for fibroblasts on 2D fibronectin. This intriguing finding identifies a striking dominance or preference for this integrin when cells interact with 3D matrix - it suggests that there may be either a hyperactivation of function of the a5ß1 integrin, a suppression of functions of other integrins, or some combination of these novel actions in an intriguing system of integrin crosstalk.

The adhesions formed by fibroblastic, mesenchymal, and neural crest cells to 3D matrices are distinct from both the focal adhesions and the fibrillar adhesions traditionally described and studied in cell culture (7,16,47,51) (Figure 2). These novel "3D-matrix adhesions" contain many of the same plaque proteins as are found in focal adhesions, such as paxillin, vinculin, and focal adhesion kinase (FAK). However, they lack the aVß3 integrin, and they are instead based on the a5ß1 integrin (Table 1).

Most intriguingly, unlike the very well-studied tyrosine-phosphorylated forms of FAK in focal adhesions, the FAK in 3D-matrix adhesions is poorly phosphorylated at its major tyrosine 397 phosphorylation site. This discrepancy in signaling in 2D versus 3D contexts is specific in that paxillin is equally phosphorylated at tyrosine 31 in both 3D-matrix adhesions and focal adhesions. Moreover, the extent of mitogen-activated protein kinase (extracellular signal-regulated kinase, ERK) phosphorylation is moderately elevated (47), which may help contribute to the enhanced rate of proliferation in 3D matrix. Differences in signaling processes have also been reported in cells suspended in three-dimensional collagen gels when compared to 2D cultures (52-54), or under different loading conditions for collagen gel contraction measurements (40-42). The important conclusion from these reports is that studies of signaling in regular cell culture may provide misleading results: cells may have different signaling responses when they exist in three-dimensional settings that mimic in vivo conditions more closely. Future studies of cell interactions with extracellular matrix and signal transduction should therefore ideally be designed with these principles in mind.


Figure 2. Comparison of fibroblast cell shapes and localization of components in 2-dimensional and 3-dimensional environments. Cells on 2D substrates tend to spread onto the substrate in a flattened morphology (left panel), whereas cells attaching to 3D matrices rapidly assume an elongated morphology (right panel) that tends to mimic shapes of fibroblastic and mesenchymal cells in vivo. The a5 integrin (red) localizes to focal and fibrillar adhesions in cells on 2D substrates coated with fibronectin (green), whereas it is organized into thin, elongated 3D-matrix adhesions in the 3D matrix containing a complex fibronectin-containing network of fibrils.

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Cellular responses to extracellular matrices - the importance of matrix rigidity

A previous analysis of the role of matrix pliability/rigidity revealed that the ability of cells to physically move and rearrange fibronectin was important in determining the nature of the cell adhesions that form on a 2D substrate. If fibronectin is cross-linked to a substrate, rather than being adsorbed and able to be remodeled, it results in exaggerated focal adhesions and trapping of the a5 integrin in these adhesions rather than translocating away to form fibrillar adhesions (55). Not surprisingly, fibronectin matrix assembly was blocked in the absence of these latter adhesions involved in generating fibronectin fibrils (30,55). This response to immobilized fibronectin may be due to the development of increased tension within cells against the fixed fibronectin substrate. Effects of local tension can be mimicked by experimentally applying external physical tension near focal adhesions, which causes enlargement of these structures; there is a dynamic relationship between the forces on adhesions and their size and function (7,56,57).

If 3D matrices are stiffened and immobilized by chemical cross-linking, a similar enhancement of focal adhesion-like structures occurs. Normal 3D-matrix adhesions are lost, and a5 integrins remain confined to focal adhesions (47). Thus, one can view the pliability or capacity for rearrangement of matrix components as being another important element of cellular responses to the extracellular matrix, along with its biochemical composition and three-dimensionality (Figure 3).


Figure 3. Cells respond to distinct physical and biochemical characteristics of the extracellular matrix. Although it is well known that cells respond in differing fashion to distinct matrix molecules (i.e., composition of the matrix), they also respond to differences in pliability of the matrix, to tension forces, and to the spatial nature of the matrix (3D versus 2D). These responses result in a variety of adhesion structures formed in response to diverse environmental cues.

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Concluding remarks

Integrin-mediated cell interactions with extracellular matrix now appear to be considerably more complex and sophisticated than originally envisioned. The existence of different types of adhesions in the same cell, as well as differences in the molecular composition of adhesions, signaling, and biological functions in two-dimensional versus three-dimensional environments (Figure 4) provides both enlightenment and a concern. It seems obvious in retrospect that different functions of cell-matrix interactions should require different types of adhesions (i.e., focal adhesions for the strongest level of adhesion and fibrillar adhesions for permitting dynamic assembly of matrix fibrils). On the other hand, focal adhesions can themselves translocate under certain conditions (59), and the existence of multiple types of adhesions forces us to re-think concepts about the actual sites from which integrin-mediated signaling may originate. A concern, which is probably healthy, is that studies of different cell types and different model matrices, whether 2D or 3D, are likely to give different answers to experimental questions as the cells utilize different types of adhesion structures. However, the comforting aspect of this complexity is that it is now clear that cells have many ways in which to accomplish the subtle variations and major changes in function that they require for maintaining normal physiological function in the intact organism and in responding to injury and other challenges.


Figure 4. Summary of cellular responses to the extracellular matrix. The responses of cells to extracellular matrix differ depending on its organization and the type of cell adhesion. Focal adhesions are localized sites of firm anchorage to a relatively rigid substrate molecule such as vitronectin, in which integrins such as aVß3 organize intracellular complexes of adhesion complex components that include focal adhesion kinase (FAK), paxillin, and tensin, as well as classical signaling molecules such as Src and ERK. In addition, novel components such as kinectin (58) are being discovered; the number of components is already greater than thirty, and more are expected. Fibrillar adhesions extend towards the cell center from the edges of focal adhesions. They consist of the a5ß1 fibronectin receptor in association with extracellular fibronectin fibrils and with intracellular tensin, talin, and actin microfilaments within a pliable extracellular substrate that consists of more than one matrix component. They tend to have low levels of tyrosine phosphorylation. Finally, when cells interact with 3D extracellular matrix, they form 3D-matrix adhesions that contain various adhesion complex proteins such as paxillin, vinculin, FAK, and tensin. Even though tyrosine 31 of paxillin is phosphorylated to levels similar to those found in cells forming extensive focal adhesions on 2D substrates, the phosphorylation of tyrosine 397 of FAK is strikingly decreased, indicating differential alterations in signaling. Cell interactions with fibronectin-rich 3D matrices are characterized by enhanced rates of cell attachment, acquisition of an elongated, spindle cell shape, cell proliferation, and motility. These interactions are mediated primarily by the a5ß1 integrin and depend on matrix pliability. Besides the difference in FAK signal transduction, there are significant effects on activation of ERK, a central regulatory mitogen-activated protein kinase.

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Correspondence and Footnotes

Address for correspondence: K.M. Yamada, CDBRB, NIDCR, NIH, Building 30, Room 421, 30 Convent Drive MSC 4370, Bethesda, MD 20892-4370, USA. Fax: +1-301-402-0897. E-mail: kenneth.yamada@nih.gov

Presented at SIMEC 2002 (International Symposium on Extracellular Matrix), Angra dos Reis, RJ, Brazil, October 7-10, 2002. Received February 27, 2003. Accepted April 15, 2003.

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