Abstract
Platycodin D (PD) is a major constituent of Platycodon grandiflorum and has multiple functions in disease control. This study focused on the function of PD in bladder cancer cell behaviors and the molecules involved. First, we administered PD to the bladder cancer cell lines T24 and 5637 and the human uroepithelial cell line SV-HUC-1. Cell viability and growth were evaluated using MTT, EdU, and colony formation assays, and cell apoptosis was determined using Hoechst 33342 staining and flow cytometry. The microRNAs (miRNAs) showing differential expression in cells before and after PD treatment were screened. Moreover, we altered the expression of miR-129-5p and PABPC1 to identify their functions in bladder cancer progression. We found that PD specifically inhibited the proliferation and promoted the apoptosis of bladder cancer cells; miR-129-5p was found to be partially responsible for the cancer-inhibiting properties of PD. PABPC1, a direct target of miR-129-5p, was abundantly expressed in T24 and 5637 cell lines and promoted cell proliferation and suppressed cell apoptosis. In addition, PABPC1 promoted the phosphorylation of PI3K and AKT in bladder cancer cells. Altogether, PD had a concentration-dependent suppressive effect on bladder cancer cell growth and was involved in the upregulation of miR-129-5p and the subsequent inhibition of PABPC1 and inactivation of PI3K/AKT signaling.
Platycodin D; Bladder cancer; microRNA-129-5p; PABPC1; PI3K/AKT signaling pathway
Retraction for: Braz J Med Biol Res | doi: 10.1590/1414-431X202010222 | PMID: 33470388 | PMCID: PMC 7814303
The authors requested retraction of the article “Suppressive effect of platycodin D on bladder cancer through microRNA-129-5p-mediated PABPC1/PI3K/AKT axis inactivation” that was published in volume 54 no. 3 (2021) (Epub January 15, 2021) in the Brazilian Journal of Medical and Biological Research.
The corresponding author stated the following: “In a subsequent experiment, we repeated the experiment in this manuscript. Unfortunately, we had different results than before. This led to inaccurate conclusions in this manuscript”.
In addition, the manuscript was questioned by independent journalists from the “PubPeer” website <https://pubpeer.com/publications/51FACD700BCEB2DE71D4748E96BE72>. This denouncement consisted of potential data falsification and/or inaccuracy.
Thus, the Editors decided to retract this article to avoid further damage to the scientific community. The Brazilian Journal of Medical and Biological Research remains vigilant to prevent misconduct and reinforces the Journal’s commitment to good scientific practices.
Publication Dates
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Publication in this collection
15 Jan 2021 -
Date of issue
2021
History
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Received
13 May 2020 -
Accepted
12 Oct 2020
