Estudo histológico e histoquímico do aparelho excretor de Glossoscolex paulistus Michaelsen, 1926 (Oligochaeta, Glossoscolecidae)

Buck, Nelson

doi:10.1590/S0101-81751985000400009

Resumo

The present study reveals that the excretory system of Glossoscolex paulistus is formed by open holonephridia (exonephridia). It is noticed that different zones are recognized in the nephridial canal, with defined histological and histochemical characteristics: 1) primary canal; 2) lobed canal; 3) first rounded segment; 4) afferent thin segment; 5) efferent thin segment; 6) second rounded segment; 7) ciliary tube; 8) clear canal; 9) pigmented canal; 10) intermediate canal; 11) bladder; 12) terminal canal; 13) nephridiopore. All these regions are intercellular and cilia are found in regions 1, 2, 3, 6, 7 and 13. The connections between afferent and efferent capillary of the vascular supply of the nephridium are made of capillary loops and dilatations which we call "glomeruli" in this paper. Filtering functions are suggested for the "glomerulus". The response of the lobed canal and the first and second rounded segments to Gomori's reaction is strongly positive and this is found to coincide with the largest concentration of "glomeruli" in the main loops 1 and II, that suggests a higher filtration capacity for the canal and segments.

Estudo histológico e histoquímico do aparelho excretor de Glossoscolex paulistus Michaelsen, 1926 (Oligochaeta, Glossoscolecidae)

Nelson Buck

Instituto de Planejamento e Estudos Ambientais, UNESP, Presidente Prudente, SP

ABSTRACT

The present study reveals that the excretory system of Glossoscolex paulistus is formed by open holonephridia (exonephridia). It is noticed that different zones are recognized in the nephridial canal, with defined histological and histochemical characteristics: 1) primary canal; 2) lobed canal; 3) first rounded segment; 4) afferent thin segment; 5) efferent thin segment; 6) second rounded segment; 7) ciliary tube; 8) clear canal; 9) pigmented canal; 10) intermediate canal; 11) bladder; 12) terminal canal; 13) nephridiopore. All these regions are intercellular and cilia are found in regions 1, 2, 3, 6, 7 and 13. The connections between afferent and efferent capillary of the vascular supply of the nephridium are made of capillary loops and dilatations which we call "glomeruli" in this paper. Filtering functions are suggested for the "glomerulus". The response of the lobed canal and the first and second rounded segments to Gomori's reaction is strongly positive and this is found to coincide with the largest concentration of "glomeruli" in the main loops 1 and II, that suggests a higher filtration capacity for the canal and segments.

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BIBLIOGRAFIA

ABREVIATURAS

Avel, M., 1959. Classe des annélides oligochètes. In: Grasse, P. Traité de Zoologie. Paris, Masson, v. 5, fasc. 1. p. 224-593.
Bahl, K. N., 1919. On a new type of nephridia found in Indian earthworms of the genus Pheretima. Q. Jl. microsc. Sci. 64: 67-117.
Bahl, K. N., 1924. Enteronephric type of nephridial system in Lampito. Q. Jl. microsc. Sci., 68: 67-69.
Bahl, K. N., 1941. The enteronephric type of nephridial system in genus Tonoscolex (Gates). Q. Jl. microsc. Sci., 82: 443-66.
Bahl, K. N., 1942. Studies on the structure, development and physiology of the nephridia of Oligochaeta. Part I: General introduction, and the nephridia of the Sub-family Octochetinae. Part II: Multiple funnels of the nephridia. Q. Jl. microsc. Sci., 83: 423-57.
Bahl, K. N., 1944. Studies on the structure, development, and physiology of the nephridia of Oligochaeta. Part III. The branching and division of nephridia, and Eisen's so-called "safety valves" in Pontoscolex. Q. Jl. microsc. Sci., 84: 1-17.
Bahl, K. N., 1947. Excretion in the Oligochaeta. Biol. Rev. of Cambridge Philos. Society., 22: 109-47.
Behmer, O. A., E. M. C. Tolosa & A. G. Freitas Neto, 1976. Manual de Técnicas para Histologia Normal e Patológica. São Paulo, Edart. 251 p.
Benham, W. B., 1891. The nephridium of Lumbricus and its blood-supply: with remarcks on the nephridia of other Chaetopoda. Q. Jl. microsc. Sci., n.s., 52: 293-334.
Buck, N., 1983. Anatomia do aparelho excretor de Glossoscolex paulistus Michaelsen, 1926 (Oligochaeta, Glossoscolecidae). Bolm Zool. Univ. S. Paulo, 6: 15-46.
Cordier, R., 1934. Études histophysiologiques sur la nephridie du Lombric. Archs. Biol, 45: 431-71.
Costa, A. C. & P. R. Chaves, 1943. Manual de técnica histológica. Lisboa, Portugália, 544 p.
Dev, B., 1965. The cytochemical localization of alkaline phosphatase in the testicular nephridia of the Indian leech, Hirudinaria granulosa with special reference to the inner lobe. Q. Jl. microsc. Sci., n.s., 106: 351-5.
El Duweini, A. K., 1940. The anatomy of Allolobophora caliginosa (Savigny, f. trapezoides (Dugès). Bull. Fac. Sci. Egypt Univ. Cairo., 21: 59-151.
Goodrich, E. S., 1933. On the nephridiostome of Lumbricus. Q. Jl. microsc. Sci., 75: 165-79.
Goodrich, E. S., 1946. The study of nephridia and genital duets since 1895. Q. Jl. microsc. Sci., 86: 113-392.
Gomori, G., 1952. Microscopic histochemistry: principles and practices. Chicago, Univ. Chicago Press. 226 p.
Graszynski, K., 1963. Die Feinstruktur des Nephridialkanals von Lumbricus terrestris L., Eine eletronenmikroskopiche Untersuchung. Zool. Beitr., 8(2): 189-296.
Graszynski, K., 1965. Biochemische und histochemische Untersuchungen an der "Alkalischen Phosphatase" in Nephridium des Regenwurms, Lumbricus terrestris L. Z. vergl. Physiol., 50: 402-4.
Johansen, K. & A. W. Martin, 1965. Circulation in a giant carthworm Glossoscolex giganteus. I. Contractile processes and pressure gradients in the large blood vessels. J. exp. Biol. 43:333-47.
Koechlin, N., 1970. Études citophysiologiques des nephridies thoraciques d'une annélide polichète (Sabelle): I. Bases ultraestructurales de la permeabilité de la paroi des branches montantes, descendantes e des dissépiments. Archs. Anat. microsc. Morph. exp., 59(4): 331-60.
Knop, J., 1926. Bakterien und Bakterioiden bei Oligochäten. Z. Morph. Ökol. Tiere., 6: 588-624.
Langeron, M. Précis de microscopie. 1934. Paris, Masson. 1205 p.
Laverack, M. S., 1963. The physiology of earthworm. New York, Mac Millan, 206 p.
Lison, L., 1960. Histochimie et cytochimie animales. Paris, Gautier-Villars, 2 v.
Ljungstrom, P. O., 1972. Biology of Glossoscolex uruguayensis (Glossoscolecidae, Oligochaeta). A new species for Argentina. Stud. Neotrop. Fauna., 7: 195-205.
Maziarski, S., 1903. Recherches cytologiques sur les organes segmentaires des vers de terre. Poln. Arch. biol. med. Wiss., 2: 3-83.
McClung, C. E., 1937. Handbook of microscopical technique. New York, Hoeber, 698 p.
McManus, J. F. A. & J. E. Cason, 1950. Carbohydrate histochemistry studies by acetilation techniques. I. Periodic acid methods. J. exp. Med., 91: 651-654.
Michaelsen, W., 1897. Organization einiger neuer oder wenig bekannter Regenwürmer von Westindien und Südamerika. Zool. Jb. (Anat)., 10: 359-88.
Neder, E. N., 1964. Introdução à microbiología: Guia de aulas práticas do Curso de Microbiologia Aplicada. Piracicaba. Instituto de Zootecnia "Prof. Jayme Rocha Almeida", ESALQ. 118 p.
Pandazis, G., 1931. Zur Frage der Bakteriensymbiose bei Oligochäten. Zentbl. Bakt. ParasitKde Abt I Orig., 120 (7/8): 440-52.
Pantin, C. F. A., 1964. Notes on microscopical technique for zoologists. Cambridge, Cambridge Univ. Press., 76 p.
Pearse, A. G. E., 1953. Histochemistry: theoretical and applied. London, Churchil, 530 p.
Perrier, E., 1874. Études sur l'organization des Lumbricides terrestres (Urochaeta). Archs Zool. exp. gén. 3: 331-530.
Pickford, G. E., 1940. An account of the anatomy of a giant earthworm from Ecuador. Turtox News, 18(17): 1-6.
Policard, A.; M. Bessis & M. Locquin, 1957. Traité de Microscopie. Paris, Masson. 608 p.
Righi, G., 1971. Sobre a Família Glossoscolecidae (Oligochaeta) no Brasil. Archs zool. Est. S. Paulo, 20(1): 1-95.
Rosen, F., 1911. Wimpertrichter der Lumbriciden (Ein Beitrag zur Kenntnis der lymphoiden Organe). Z. wiss. Zool., 98: 135-78.
Scott, D. A. & A. J. Musgrave, 1973. Aspects of the fine structure of symbionts and related host tissues in nephridia of Allolobophora caliginosa typica (Annelida: Lumbricidae) J. invertebr. Pathol., 6(5): 515-19.
Stephenson, J., 1930. The Oligochaeta. Oxford, Clarendon Press. 978 p.
Vanderbrock, G., 1934. Organogènese du système nephridien chez Eisenia foetida Sav. Recl. Inst. zool. Torley-Rousseau, 5: 5-72.
Vinichenko, L. N. & V. K. Lebskii, 1975. Comparative ultraestructural analysis of the protonephridium of Eteone longa and the metanephridium of Lumbricus terrestris. Tsitologiya, 17(6): 615-19.
Zerbst-Borofka, I., 1975. Function and ultrastructure of the nephridium in Hirudo medicinalis L.: III: Mechanisms of the formation of primary and final urine. J. Comp. Physiol. B. Metb. Trans. Funct., 100(4): 307-316.

Datas de Publicação

Publicação nesta coleção
21 Ago 2009
Data do Fascículo
1985

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] Avel, M., 1959. Classe des annélides oligochètes. In: Grasse, P. Traité de Zoologie. Paris, Masson, v. 5, fasc. 1. p. 224-593.

[2] Bahl, K. N., 1919. On a new type of nephridia found in Indian earthworms of the genus Pheretima. Q. Jl. microsc. Sci. 64: 67-117.

[3] Bahl, K. N., 1924. Enteronephric type of nephridial system in Lampito. Q. Jl. microsc. Sci., 68: 67-69.

[4] Bahl, K. N., 1941. The enteronephric type of nephridial system in genus Tonoscolex (Gates). Q. Jl. microsc. Sci., 82: 443-66.

[5] Bahl, K. N., 1942. Studies on the structure, development and physiology of the nephridia of Oligochaeta. Part I: General introduction, and the nephridia of the Sub-family Octochetinae. Part II: Multiple funnels of the nephridia. Q. Jl. microsc. Sci., 83: 423-57.

[6] Bahl, K. N., 1944. Studies on the structure, development, and physiology of the nephridia of Oligochaeta. Part III. The branching and division of nephridia, and Eisen's so-called "safety valves" in Pontoscolex. Q. Jl. microsc. Sci., 84: 1-17.

[7] Bahl, K. N., 1947. Excretion in the Oligochaeta. Biol. Rev. of Cambridge Philos. Society., 22: 109-47.

[8] Behmer, O. A., E. M. C. Tolosa & A. G. Freitas Neto, 1976. Manual de Técnicas para Histologia Normal e Patológica. São Paulo, Edart. 251 p.

[9] Benham, W. B., 1891. The nephridium of Lumbricus and its blood-supply: with remarcks on the nephridia of other Chaetopoda. Q. Jl. microsc. Sci., n.s., 52: 293-334.

[10] Buck, N., 1983. Anatomia do aparelho excretor de Glossoscolex paulistus Michaelsen, 1926 (Oligochaeta, Glossoscolecidae). Bolm Zool. Univ. S. Paulo, 6: 15-46.

[11] Cordier, R., 1934. Études histophysiologiques sur la nephridie du Lombric. Archs. Biol, 45: 431-71.

[12] Costa, A. C. & P. R. Chaves, 1943. Manual de técnica histológica. Lisboa, Portugália, 544 p.

[13] Dev, B., 1965. The cytochemical localization of alkaline phosphatase in the testicular nephridia of the Indian leech, Hirudinaria granulosa with special reference to the inner lobe. Q. Jl. microsc. Sci., n.s., 106: 351-5.

[14] El Duweini, A. K., 1940. The anatomy of Allolobophora caliginosa (Savigny, f. trapezoides (Dugès). Bull. Fac. Sci. Egypt Univ. Cairo., 21: 59-151.

[15] Goodrich, E. S., 1933. On the nephridiostome of Lumbricus. Q. Jl. microsc. Sci., 75: 165-79.

[16] Goodrich, E. S., 1946. The study of nephridia and genital duets since 1895. Q. Jl. microsc. Sci., 86: 113-392.

[17] Gomori, G., 1952. Microscopic histochemistry: principles and practices. Chicago, Univ. Chicago Press. 226 p.

[18] Graszynski, K., 1963. Die Feinstruktur des Nephridialkanals von Lumbricus terrestris L., Eine eletronenmikroskopiche Untersuchung. Zool. Beitr., 8(2): 189-296.

[19] Graszynski, K., 1965. Biochemische und histochemische Untersuchungen an der "Alkalischen Phosphatase" in Nephridium des Regenwurms, Lumbricus terrestris L. Z. vergl. Physiol., 50: 402-4.

[20] Johansen, K. & A. W. Martin, 1965. Circulation in a giant carthworm Glossoscolex giganteus. I. Contractile processes and pressure gradients in the large blood vessels. J. exp. Biol. 43:333-47.

[21] Koechlin, N., 1970. Études citophysiologiques des nephridies thoraciques d'une annélide polichète (Sabelle): I. Bases ultraestructurales de la permeabilité de la paroi des branches montantes, descendantes e des dissépiments. Archs. Anat. microsc. Morph. exp., 59(4): 331-60.

[22] Knop, J., 1926. Bakterien und Bakterioiden bei Oligochäten. Z. Morph. Ökol. Tiere., 6: 588-624.

[23] Langeron, M. Précis de microscopie. 1934. Paris, Masson. 1205 p.

[24] Laverack, M. S., 1963. The physiology of earthworm. New York, Mac Millan, 206 p.

[25] Lison, L., 1960. Histochimie et cytochimie animales. Paris, Gautier-Villars, 2 v.

[26] Ljungstrom, P. O., 1972. Biology of Glossoscolex uruguayensis (Glossoscolecidae, Oligochaeta). A new species for Argentina. Stud. Neotrop. Fauna., 7: 195-205.

[27] Maziarski, S., 1903. Recherches cytologiques sur les organes segmentaires des vers de terre. Poln. Arch. biol. med. Wiss., 2: 3-83.

[28] McClung, C. E., 1937. Handbook of microscopical technique. New York, Hoeber, 698 p.

[29] McManus, J. F. A. & J. E. Cason, 1950. Carbohydrate histochemistry studies by acetilation techniques. I. Periodic acid methods. J. exp. Med., 91: 651-654.

[30] Michaelsen, W., 1897. Organization einiger neuer oder wenig bekannter Regenwürmer von Westindien und Südamerika. Zool. Jb. (Anat)., 10: 359-88.

[31] Neder, E. N., 1964. Introdução à microbiología: Guia de aulas práticas do Curso de Microbiologia Aplicada. Piracicaba. Instituto de Zootecnia "Prof. Jayme Rocha Almeida", ESALQ. 118 p.

[32] Pandazis, G., 1931. Zur Frage der Bakteriensymbiose bei Oligochäten. Zentbl. Bakt. ParasitKde Abt I Orig., 120 (7/8): 440-52.

[33] Pantin, C. F. A., 1964. Notes on microscopical technique for zoologists. Cambridge, Cambridge Univ. Press., 76 p.

[34] Pearse, A. G. E., 1953. Histochemistry: theoretical and applied. London, Churchil, 530 p.

[35] Perrier, E., 1874. Études sur l'organization des Lumbricides terrestres (Urochaeta). Archs Zool. exp. gén. 3: 331-530.

[36] Pickford, G. E., 1940. An account of the anatomy of a giant earthworm from Ecuador. Turtox News, 18(17): 1-6.

[37] Policard, A.; M. Bessis & M. Locquin, 1957. Traité de Microscopie. Paris, Masson. 608 p.

[38] Righi, G., 1971. Sobre a Família Glossoscolecidae (Oligochaeta) no Brasil. Archs zool. Est. S. Paulo, 20(1): 1-95.

[39] Rosen, F., 1911. Wimpertrichter der Lumbriciden (Ein Beitrag zur Kenntnis der lymphoiden Organe). Z. wiss. Zool., 98: 135-78.

[40] Scott, D. A. & A. J. Musgrave, 1973. Aspects of the fine structure of symbionts and related host tissues in nephridia of Allolobophora caliginosa typica (Annelida: Lumbricidae) J. invertebr. Pathol., 6(5): 515-19.

[41] Stephenson, J., 1930. The Oligochaeta. Oxford, Clarendon Press. 978 p.

[42] Vanderbrock, G., 1934. Organogènese du système nephridien chez Eisenia foetida Sav. Recl. Inst. zool. Torley-Rousseau, 5: 5-72.

[43] Vinichenko, L. N. & V. K. Lebskii, 1975. Comparative ultraestructural analysis of the protonephridium of Eteone longa and the metanephridium of Lumbricus terrestris. Tsitologiya, 17(6): 615-19.

[44] Zerbst-Borofka, I., 1975. Function and ultrastructure of the nephridium in Hirudo medicinalis L.: III: Mechanisms of the formation of primary and final urine. J. Comp. Physiol. B. Metb. Trans. Funct., 100(4): 307-316.

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Estudo histológico e histoquímico do aparelho excretor de Glossoscolex paulistus Michaelsen, 1926 (Oligochaeta, Glossoscolecidae)

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