Histologia y morfologia del sistema reproductor en hembras de Rhagoletis conversa (Brethes) y de Rhagoletis nova (Schimer) (Diptera, Tephritidae)

Flores Rojas, Verónica; Lasserre, Daniel Frías; Martínes Arteaga, Humberto

doi:10.1590/S0101-81751987000100003

Resumen

The histology and morphology of the reproductive system of females of Rhagoletis conversa (Brèthes) and Rhagoletis nova (Schiner) were studied. These species parasitize wild and cultivated Solanaceae respectively. Adult females of both species were collected in natural populations in the Central Zone of Chile. In order to study the development of ovaries the females collected were sacrificed at different ages. Once obtained, ovaries were preparedfor optical microscopy by using histological and squash standard techniques. Results show that within each one of ovarioles it can be observed follicles arranged in line and showing different stages of maduration. It can be described about 6 follicle stages in the ovarioles studied. Ovaries of both species show a very similar structure. However, females of Rhagoletis nova show an ovipositor wider than R. conversa. The number of ovarioles of R. nova is also higher than in R. conversa.

Histologia y morfologia del sistema reproductor en hembras de Rhagoletis conversa (Brethes) y de Rhagoletis nova (Schimer) (Diptera, Tephritidae)

Verónica Flores Rojas; Daniel Frías Lasserre; Humberto Martínes Arteaga

Departamento de Biologia Celular y Genética, Facultad de Medicina, Universidad de Chile, Casilla 70061, Correo 7, Santiago, Chile

ABSTRACT

The histology and morphology of the reproductive system of females of Rhagoletis conversa (Brèthes) and Rhagoletis nova (Schiner) were studied. These species parasitize wild and cultivated Solanaceae respectively.

Adult females of both species were collected in natural populations in the Central Zone of Chile. In order to study the development of ovaries the females collected were sacrificed at different ages. Once obtained, ovaries were preparedfor optical microscopy by using histological and squash standard techniques.

Results show that within each one of ovarioles it can be observed follicles arranged in line and showing different stages of maduration. It can be described about 6 follicle stages in the ovarioles studied.

Ovaries of both species show a very similar structure. However, females of Rhagoletis nova show an ovipositor wider than R. conversa. The number of ovarioles of R. nova is also higher than in R. conversa.

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AGRADECIMIENTOS

Este trabajo fue financiado a través del Proyecto B 1856-8523 D.I.B. Universidad de Chile

Anderson, D.T. & Lyford, G.C. 1965. "Oogenesis in Dacus tryoni (Diptera: Tephritidae)". Aust. J. Zool 13:423-435.
Bateman, M.A. 1972. "The ecology of fruit flies". Ann. Rev. Ent. 17:493-518.
Boller, E.F. & Prokopy, R.J. 1976. "Bionomic and management of Rhagoletis". Ann Rev. Ent. 21:223-246.
Carpinelli, A.F. 1958. "Estudio biológico sobre la mosca del tomate en Chile". Tesis de prueba presentada a la Facultad de Agromía, Universidade Católica de Chile, como requisito para optar al título de Ingeniero Agrónomo (298 e. Referencia Biblioteca Facultad de Agronomía, Universidade Católica de Chile).
⁵
Christenson, L.D. & Foote, R.H. 1960. "Biology of fruit flies" Ann. Rev. Ent. 5:171-192.
Davey, K.G. 1965. "The female system and the eggs"; in: "Reproduction in the Insects". (Oliver and Boyd, ed.) London. p. 13-28.
Dean, R.W. 1935. "Anatomy and postpupal development of the female reproductive system in the apple maggot fly Rhagoletis pomonella". New York State Agriculture Experiment Station, Technical Bulletin Nº 229.
Dodson, G. 1978. "Morphology of the reproductive system in Anastrepha suspensa (Loew) and notes on related species". The Florida Entomologist. 61 (4): 231-238.
Drew, R.A. 1969, "Morphology of the reproductive system of Strumeta tryoni, with amethod of distinguishing sexually matute adult males". Jour. Aust. Ent. Soc. 8:21-32.
Foote, R.H. & Blanc, F.L. 1963. "The fruit flies or Tephritidae of California". Bull. Calif. Insect. Surv. 7:1-117.
Foote, R.H. 1967. "A catalogue of the Diptera of the America South of the United States" Depto. de Zoología, Secretaría de Agricultura, São Paulo. 57:1-91.
Foote, R.H. 1981. "The genus Rhagoletis Loew south of United States (Diptera Tephritidae)". United States Department of Agriculture. Technical Bulletin Nº 1607. 75 pp.
Frías, L.D. 1981. "Biología evolutiva de dípteros Otitidae y Tephritidae (Diptera Acalyptratae)". Tesis de Doctorado, Universidad de Chile, Santiago, Chile.
Frías, L.D. 1982 "Genética ecológica de insectos fitófagos y sus huéspedes". EN: Cruz-Coke, R. y D. Brncic (eds.). Actas V Congreso Latinoamericano de Genética. Imprenta Calderón, Santiago, pp. 256-271.
¹⁵
Frías, L.D. y Martínez, H. 1982. "Aspectos de la Biologíua evolutiva en Rhagoletis (Diptera: Tephritidae)" XV Reunión Anual de la Sociedad de Genética de Chile. Arch. Biol. y Med. Exp. 15 (2):R-173.
Frías, L.D. 1983. "Aspectos genéticos y ecológicos de la especiación simpátrida en insectos fitófagos". IX Congreso Latinoamericano de Zoología, Arequipa, Perú. Resúmenes de las comunicaciones científicas.
Frías, L.D.; Malavasi, A. & Morgante, J.S. "Field observations of distribution and activities of Rhagoletis conversa (Diptera Tephritidae) on two hosts in nature". Ann. Entom. Soc. of Am. (USA.) 77 (5): 548-551.
King, et al. 1975 "The cell cycle and cell differentiation in the Drosophila ovary", in: "Cell cycle mid cell differentiation". (W. Beerman, ed). Springer, Berlin pp. 85-109.
López, M.A.; Leyton, C.M. & Graf, M.V. 1982. "Coloraciones núcleo citoplasmáticas, en: "Técnicas de Histología y Citología". Departamento de Biología Celular y Genética, Fac. de Medicina, Universidad de Chile, (ed.) Santiago pp. 36.
Malavasi, A., J.S. Morgante, & R.A. Zucchi. 1980. "Biologia de moscas-das-frutas (Diptera: Tephritidae) I Lista de hospedeiros e ocorrência". Rev. Brasil. Biol. 40 (1): 9-16.
Malavasi, A. & Morgante, J.S. 1980. "Biologia de moscas-das-frutas" (Diptera: Tephritidae). II Índices de infestação em diferentes hospedeiros e localidades. Rev. Brasil. Biol. 40 (1): 17-24.
Malavasi, A. & Morgante, J.S. 1981. "Adult and larval population fluctuation in Anastrepha fraterculus and it relation host availability". Env. Ent 10: 275-278.
Miller, A. 1950. "The female reproductive system", in: Biology of Drosophila (M. Demerec, ed.) New York. pp. 518-530.
Nonidez, J.F. 1920. "The intemal phenomena of reproduction in Drosophila". Biol. Bull. 39: 201-230.

Fechas de Publicación

Publicación en esta colección
20 Ago 2009
Fecha del número
1987

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] Anderson, D.T. & Lyford, G.C. 1965. "Oogenesis in Dacus tryoni (Diptera: Tephritidae)". Aust. J. Zool 13:423-435.

[2] Bateman, M.A. 1972. "The ecology of fruit flies". Ann. Rev. Ent. 17:493-518.

[3] Boller, E.F. & Prokopy, R.J. 1976. "Bionomic and management of Rhagoletis". Ann Rev. Ent. 21:223-246.

[4] Carpinelli, A.F. 1958. "Estudio biológico sobre la mosca del tomate en Chile". Tesis de prueba presentada a la Facultad de Agromía, Universidade Católica de Chile, como requisito para optar al título de Ingeniero Agrónomo (298 e. Referencia Biblioteca Facultad de Agronomía, Universidade Católica de Chile).

[5] ⁵
Christenson, L.D. & Foote, R.H. 1960. "Biology of fruit flies" Ann. Rev. Ent. 5:171-192.

[6] Davey, K.G. 1965. "The female system and the eggs"; in: "Reproduction in the Insects". (Oliver and Boyd, ed.) London. p. 13-28.

[7] Dean, R.W. 1935. "Anatomy and postpupal development of the female reproductive system in the apple maggot fly Rhagoletis pomonella". New York State Agriculture Experiment Station, Technical Bulletin Nº 229.

[8] Dodson, G. 1978. "Morphology of the reproductive system in Anastrepha suspensa (Loew) and notes on related species". The Florida Entomologist. 61 (4): 231-238.

[9] Drew, R.A. 1969, "Morphology of the reproductive system of Strumeta tryoni, with amethod of distinguishing sexually matute adult males". Jour. Aust. Ent. Soc. 8:21-32.

[10] Foote, R.H. & Blanc, F.L. 1963. "The fruit flies or Tephritidae of California". Bull. Calif. Insect. Surv. 7:1-117.

[11] Foote, R.H. 1967. "A catalogue of the Diptera of the America South of the United States" Depto. de Zoología, Secretaría de Agricultura, São Paulo. 57:1-91.

[12] Foote, R.H. 1981. "The genus Rhagoletis Loew south of United States (Diptera Tephritidae)". United States Department of Agriculture. Technical Bulletin Nº 1607. 75 pp.

[13] Frías, L.D. 1981. "Biología evolutiva de dípteros Otitidae y Tephritidae (Diptera Acalyptratae)". Tesis de Doctorado, Universidad de Chile, Santiago, Chile.

[14] Frías, L.D. 1982 "Genética ecológica de insectos fitófagos y sus huéspedes". EN: Cruz-Coke, R. y D. Brncic (eds.). Actas V Congreso Latinoamericano de Genética. Imprenta Calderón, Santiago, pp. 256-271.

[15] ¹⁵
Frías, L.D. y Martínez, H. 1982. "Aspectos de la Biologíua evolutiva en Rhagoletis (Diptera: Tephritidae)" XV Reunión Anual de la Sociedad de Genética de Chile. Arch. Biol. y Med. Exp. 15 (2):R-173.

[16] Frías, L.D. 1983. "Aspectos genéticos y ecológicos de la especiación simpátrida en insectos fitófagos". IX Congreso Latinoamericano de Zoología, Arequipa, Perú. Resúmenes de las comunicaciones científicas.

[17] Frías, L.D.; Malavasi, A. & Morgante, J.S. "Field observations of distribution and activities of Rhagoletis conversa (Diptera Tephritidae) on two hosts in nature". Ann. Entom. Soc. of Am. (USA.) 77 (5): 548-551.

[18] King, et al. 1975 "The cell cycle and cell differentiation in the Drosophila ovary", in: "Cell cycle mid cell differentiation". (W. Beerman, ed). Springer, Berlin pp. 85-109.

[19] López, M.A.; Leyton, C.M. & Graf, M.V. 1982. "Coloraciones núcleo citoplasmáticas, en: "Técnicas de Histología y Citología". Departamento de Biología Celular y Genética, Fac. de Medicina, Universidad de Chile, (ed.) Santiago pp. 36.

[20] Malavasi, A., J.S. Morgante, & R.A. Zucchi. 1980. "Biologia de moscas-das-frutas (Diptera: Tephritidae) I Lista de hospedeiros e ocorrência". Rev. Brasil. Biol. 40 (1): 9-16.

[21] Malavasi, A. & Morgante, J.S. 1980. "Biologia de moscas-das-frutas" (Diptera: Tephritidae). II Índices de infestação em diferentes hospedeiros e localidades. Rev. Brasil. Biol. 40 (1): 17-24.

[22] Malavasi, A. & Morgante, J.S. 1981. "Adult and larval population fluctuation in Anastrepha fraterculus and it relation host availability". Env. Ent 10: 275-278.

[23] Miller, A. 1950. "The female reproductive system", in: Biology of Drosophila (M. Demerec, ed.) New York. pp. 518-530.

[24] Nonidez, J.F. 1920. "The intemal phenomena of reproduction in Drosophila". Biol. Bull. 39: 201-230.

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Histologia y morfologia del sistema reproductor en hembras de Rhagoletis conversa (Brethes) y de Rhagoletis nova (Schimer) (Diptera, Tephritidae)

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Fechas de Publicación