Estudo morfológico e quantitativo dos hemócitos em larvas de Diatraea saccharalis (Fabricius) (Lepidoptera, Pyralidae)

Bombonato, Maria T. Siqueira; Gregório, Elisa A

doi:10.1590/S0101-81751995000400016

Resumo

Hemocytes of Diatraea saccharalis larvae aging from 13 to 26 days old were morphological and quantitatively characterized by phase contrast microscopy. Six hemocyte types were described and identified as: prohemocytes (PR), plasmatocytes (PL), granulocytes (GR), oenocytoides (OE). spherulocytes (SP) and verm cells (VE). The total hemocyte counts showed no significant difference in the number of these cells along the normal development of the larvae. However, the relative number of different circulating hemocyte types fluctuated, and it could be expressed as: PL > GR > SP> PR > VE > OE in larvae up to 14 days old; and as: GR > SP > PL > VE > OE > PR in those older than 24 days.

Lepidoptera; Pyralidae; Diatraea saccharalis; total hemocyte count; differential hemocyte count; hemolymph

Estudo morfológico e quantitativo dos hemócitos em larvas de Diatraea saccharalis (Fabricius) (Lepidoptera, Pyralidae)

Morphological and quantitative studies of the hemocytes in larvae of Diatraea saccharalis (Fabricius) (Lepidoptera, Pyralidae)

Maria T. Siqueira Bombonato^I; Elisa A. Gregório^II

^IDepartamento de Ciências Biológicas. Faculdade de Ciências. Universidade Estadual Paulista. Caixa Postal 473, 17033-360 Bauru, São Paulo, Brasil

^IIDepartamento de Morfologia. Instituto de Biociências. Universidade Estadual Paulista. Caixa Postal 501, 18618-000 Botucatu, São Paulo, Brasil

ABSTRACT

Hemocytes of Diatraea saccharalis larvae aging from 13 to 26 days old were morphological and quantitatively characterized by phase contrast microscopy. Six hemocyte types were described and identified as: prohemocytes (PR), plasmatocytes (PL), granulocytes (GR), oenocytoides (OE). spherulocytes (SP) and verm cells (VE). The total hemocyte counts showed no significant difference in the number of these cells along the normal development of the larvae. However, the relative number of different circulating hemocyte types fluctuated, and it could be expressed as: PL > GR > SP> PR > VE > OE in larvae up to 14 days old; and as: GR > SP > PL > VE > OE > PR in those older than 24 days.

Key words: Lepidoptera, Pyralidae, Diatraea saccharalis, total hemocyte count, differential hemocyte count, hemolymph

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AGRADECIMENTOS. Os autores agradecem ao Laboratório de Controle de Pragas da Usina da Barra Açúcar e Álcool S.A., pelo fornecimento das larvas e do meio de cultura, utilizados para a realização deste trabalho.

Recebido em 16.I.1995; aceito em 01.IV.1996.

Ahmad, A. 1993. Effect of β-ecdysone ingestion on total and differential haemocyte counts (THC & DHC) in the tobacco Caterpillar, Spodoptera litura Fabr. (Lepidoptera: Noctuidae). Bull. Soc. Ent. 66:113-122.
Arnold, J.W. & H. Hinks. 1976. Haemopoiesis in Lepidoptera. I. The multiplication of circulating haemocytes. Can. Jour. Zool. 54:1003-1012.
Azambuja, P.; E.S. Garcia & N.A. Ratcliffe. 1991. Aspects of classification of Hemiptera hemocytes from six Triatomine species. Mem. Inst. Oswaldo Cruz. 86:1-10.
Barduco, M.C.; E.A. Gregório & L.A. Toledo. 1988. Hemócitos de Diatraea saccharalis (Lepidoptera: Pyralidae) no período larval. Estudo morfológico e quantitativo. Rev. Bras. Biol. 48:925-932.
Beaulaton, J. 1968. La tunica propria et ses relations avec les fibres conjunetives et les hémocytes. Jour. Ultrastruct. Res. 23:474-498.
Bohn, H. 1986. Hemolymph clotting in insects, p. 188-207. In: M. Brehélin (Ed.). Immunity Invertebrates. New York, Spring-Verlag, 233p.
Chiang, A.S.; A.P. Gupta & S.S. Han. 1988. Arthropod immune system: I. Comparative light and electron microscopic accounts of immunocytes and other hemocytes of Blattella germanica (Dictyoptera: Blattellidae). Jour. Morphol. 198:257-267.
Costa-Neto, P.L.O. 1984. Estatística. São Paulo, Editora Blucher, 264p.
De Kort, C.A. & N.A. Granger. 1981. Regulation of the juvenile hormone titer. Ann. Rev. Ent. 26:1-28.
Gotz, P. & H.G. Boman. 1985. Insect immunity, p. 454-485. In: G.A. Kertut & L.I. Gilbert (Eds). Comprehensive insect physiology, biochemistry and pharmacology. Oxford, Pergamon Press, vol. 3., 625p.
Gupta, A.P. 1979. Hemocyte types; their structures, synonymies, interrelationships and taxonomic significance, p. 85-127. In: A.P. Gupta (Ed). Insect hemocytes: development, forms, functions and techniques. Cambridge, Cambridge University Press, 614p.
______. 1985. Cellular elements in the hemolymph, p. 402-444. In: G.A. Kertut & L.I. Gilbert (Eds). Comprehensive insect physiology, biochemistry and pharmacology. Oxford, Pergamon Press, vol. 3., 625p.
Hensley, S.D. & A. M. JR. Hammond. 1968. Laboratory techniques for rearing the sugarcane borer on an artificial diet. Jour. Entomol. 61:1742-1743.
Jones, J.C. 1959. A phase contrast study of the blood-cells in Prodenia larvae (Order Lepidoptera). Q. Jour. Microscop. Sci. 100:17-23.
______. 1962. Current concepts concerning insect hemocytes. Amer. Jour. Zool. 2:209-246.
Kaaya, G.P. & N.A. Ratcliffe. 1982. Comparative study of hemocytes and associated cells of some medically important dipterans. Jour. Morphol. 173:351-365.
Kurihara, Y.; T. Shimazut & H. Wago. 1992. Classification of hemocytes in the common cutworm, Spodoptera litura (Lepidoptera: Noctuidae) I. Phase microscopic study. Appl. Entomol. Zool. 27:225-235.
Lea, M.S. & L.I. Gilbert. 1966. The haemocytes of Hyalophora cecropia (Lepidoptera). Jour. Morphol. 118:197-216.
Leonard, C.M.; K. Soderhall & N.A. Ratcliffe. 1985. Studies of prophenoloxidase and protease of Blaberus craniifer haemocytes. Insect Biochem. 15:803-810.
Nittono, Y. 1960. Studies on the blood cells in the silkworm Bombyx mori L. Bull. Seric. Exp. Stn. 16:171-266.
Percy, J. 1978. Haemocytes associated with basement membrane of sex pheromone gland of Trichoplusia ni (Lepidoptera: Noctuidae). Ultrastructural observations. Canad. Jour. Zool. 56:238-245.
Price, C.D. & N.A Ratcliffe. 1974. A reappraisal of insect haemocyte classification by the examination of blood from fifteen insect orders. Z. Zellforsch. Mikrosk. Anat. 147:537-549.
Ratcliffe, N.A. 1986. Insect cellular immunity and the recognition of foreigness, p. 21-43. In: A.M. Lackie (Ed.). Immune mechanism in invertebrate vectors. Oxford, Claredon Press, 285p.
Ratcliffe, N.A. & A.F. Rowley. 1979. Role of hemocytes in defense against biological agents, p. 331-414. In: A.P. Gupta (Ed.). Insect hemocytes: development, forms, functions and techniques. Cambridge, Cambridge University Press, 614p.
Ratcliffe, N.A.; A.F. Rowley; S.W. Fitzgerald & C.P. Rhodes. 1985. Invertebrate immunity: basic concepts and recent advances. Int. Rev. Cytol. 97:183-349.
Saxena, B.P. 1992. Comparative study of haemocytes of three lepidopterans by light and scanning electron microscopy. Acta Entomol. Bohemoslov. 89:323-339.
Secco, V.N.D.P. 1990. Ultra-estrutura do desenvolvimento do ovário de Dermatobia hominis (Diptera: Cuterebridae). Dissertação de Mestrado, não publicada, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, 71p.
Shapiro, M. 1979. Changes in the hemocyte population, p. 475-523. In: A.P. Gupta (Ed.). Insect hemocytes: development, forms, functions and techniques. Cambridge, Cambridge University Press, 614p.
Srdic, Z. & H. Gloor. 1979. Spherulle cells in Drosophila species. Experientia. 35:1246-1249.
Takada, M. & H. Kittano. 1971. Studies on the larval hemocytes in the cabbage with butterfly, Pieris rapae crucivora (Boisduval), with special reference to hemocyte classifications, phagocytic activity and encapsulative capacity. Kontyú. 39:385-394.
Takeda, N. 1977. Brain hormone carrier haemocytes in the moth Monema flavescens. Jour. Insect Physiol. 23:1245-1254.
Toledo, L.A. & E.A. Gregório. 1983. Ultraestrutura dos hemócitos de larvas de Diatraea saccharalis (Lepidoptera: Pyralidae). Resumos do III Congresso Brasileiro de Biologia Celular, São Paulo, p. 47.
Vieira, S. & R. Hoffmann. 1988. Elementos de Estatística. São Paulo, Editora Atlas, 159p.
Wigglesworth, V.B. 1959. Insect blood cells. Ann. Rev. Entomol. 4:1-16.
______. 1972. Haemocytes and basement membrane formation in Rhodnius. Jour. Insect Physiol. 19:831-844.
______. 1979a. Hemocytes and growth in insects, p. 303-318. In: A.P. Gupta (Ed.). Insect hemocytes: development, forms, functions and techniques. Cambridge, Cambridge University Press, 614p.
______. 1979b. Secretory activities of plasmatocytes and oenocytoids during the moulting cycle in an insect (Rhodnius). Tissue Cell. 11:69-78.

Datas de Publicação

Publicação nesta coleção
21 Jul 2009
Data do Fascículo
1995

Histórico

Recebido
16 Jan 1995
Aceito
01 Abr 1996

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] Ahmad, A. 1993. Effect of β-ecdysone ingestion on total and differential haemocyte counts (THC & DHC) in the tobacco Caterpillar, Spodoptera litura Fabr. (Lepidoptera: Noctuidae). Bull. Soc. Ent. 66:113-122.

[2] Arnold, J.W. & H. Hinks. 1976. Haemopoiesis in Lepidoptera. I. The multiplication of circulating haemocytes. Can. Jour. Zool. 54:1003-1012.

[3] Azambuja, P.; E.S. Garcia & N.A. Ratcliffe. 1991. Aspects of classification of Hemiptera hemocytes from six Triatomine species. Mem. Inst. Oswaldo Cruz. 86:1-10.

[4] Barduco, M.C.; E.A. Gregório & L.A. Toledo. 1988. Hemócitos de Diatraea saccharalis (Lepidoptera: Pyralidae) no período larval. Estudo morfológico e quantitativo. Rev. Bras. Biol. 48:925-932.

[5] Beaulaton, J. 1968. La tunica propria et ses relations avec les fibres conjunetives et les hémocytes. Jour. Ultrastruct. Res. 23:474-498.

[6] Bohn, H. 1986. Hemolymph clotting in insects, p. 188-207. In: M. Brehélin (Ed.). Immunity Invertebrates. New York, Spring-Verlag, 233p.

[7] Chiang, A.S.; A.P. Gupta & S.S. Han. 1988. Arthropod immune system: I. Comparative light and electron microscopic accounts of immunocytes and other hemocytes of Blattella germanica (Dictyoptera: Blattellidae). Jour. Morphol. 198:257-267.

[8] Costa-Neto, P.L.O. 1984. Estatística. São Paulo, Editora Blucher, 264p.

[9] De Kort, C.A. & N.A. Granger. 1981. Regulation of the juvenile hormone titer. Ann. Rev. Ent. 26:1-28.

[10] Gotz, P. & H.G. Boman. 1985. Insect immunity, p. 454-485. In: G.A. Kertut & L.I. Gilbert (Eds). Comprehensive insect physiology, biochemistry and pharmacology. Oxford, Pergamon Press, vol. 3., 625p.

[11] Gupta, A.P. 1979. Hemocyte types; their structures, synonymies, interrelationships and taxonomic significance, p. 85-127. In: A.P. Gupta (Ed). Insect hemocytes: development, forms, functions and techniques. Cambridge, Cambridge University Press, 614p.

[12] ______. 1985. Cellular elements in the hemolymph, p. 402-444. In: G.A. Kertut & L.I. Gilbert (Eds). Comprehensive insect physiology, biochemistry and pharmacology. Oxford, Pergamon Press, vol. 3., 625p.

[13] Hensley, S.D. & A. M. JR. Hammond. 1968. Laboratory techniques for rearing the sugarcane borer on an artificial diet. Jour. Entomol. 61:1742-1743.

[14] Jones, J.C. 1959. A phase contrast study of the blood-cells in Prodenia larvae (Order Lepidoptera). Q. Jour. Microscop. Sci. 100:17-23.

[15] ______. 1962. Current concepts concerning insect hemocytes. Amer. Jour. Zool. 2:209-246.

[16] Kaaya, G.P. & N.A. Ratcliffe. 1982. Comparative study of hemocytes and associated cells of some medically important dipterans. Jour. Morphol. 173:351-365.

[17] Kurihara, Y.; T. Shimazut & H. Wago. 1992. Classification of hemocytes in the common cutworm, Spodoptera litura (Lepidoptera: Noctuidae) I. Phase microscopic study. Appl. Entomol. Zool. 27:225-235.

[18] Lea, M.S. & L.I. Gilbert. 1966. The haemocytes of Hyalophora cecropia (Lepidoptera). Jour. Morphol. 118:197-216.

[19] Leonard, C.M.; K. Soderhall & N.A. Ratcliffe. 1985. Studies of prophenoloxidase and protease of Blaberus craniifer haemocytes. Insect Biochem. 15:803-810.

[20] Nittono, Y. 1960. Studies on the blood cells in the silkworm Bombyx mori L. Bull. Seric. Exp. Stn. 16:171-266.

[21] Percy, J. 1978. Haemocytes associated with basement membrane of sex pheromone gland of Trichoplusia ni (Lepidoptera: Noctuidae). Ultrastructural observations. Canad. Jour. Zool. 56:238-245.

[22] Price, C.D. & N.A Ratcliffe. 1974. A reappraisal of insect haemocyte classification by the examination of blood from fifteen insect orders. Z. Zellforsch. Mikrosk. Anat. 147:537-549.

[23] Ratcliffe, N.A. 1986. Insect cellular immunity and the recognition of foreigness, p. 21-43. In: A.M. Lackie (Ed.). Immune mechanism in invertebrate vectors. Oxford, Claredon Press, 285p.

[24] Ratcliffe, N.A. & A.F. Rowley. 1979. Role of hemocytes in defense against biological agents, p. 331-414. In: A.P. Gupta (Ed.). Insect hemocytes: development, forms, functions and techniques. Cambridge, Cambridge University Press, 614p.

[25] Ratcliffe, N.A.; A.F. Rowley; S.W. Fitzgerald & C.P. Rhodes. 1985. Invertebrate immunity: basic concepts and recent advances. Int. Rev. Cytol. 97:183-349.

[26] Saxena, B.P. 1992. Comparative study of haemocytes of three lepidopterans by light and scanning electron microscopy. Acta Entomol. Bohemoslov. 89:323-339.

[27] Secco, V.N.D.P. 1990. Ultra-estrutura do desenvolvimento do ovário de Dermatobia hominis (Diptera: Cuterebridae). Dissertação de Mestrado, não publicada, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, 71p.

[28] Shapiro, M. 1979. Changes in the hemocyte population, p. 475-523. In: A.P. Gupta (Ed.). Insect hemocytes: development, forms, functions and techniques. Cambridge, Cambridge University Press, 614p.

[29] Srdic, Z. & H. Gloor. 1979. Spherulle cells in Drosophila species. Experientia. 35:1246-1249.

[30] Takada, M. & H. Kittano. 1971. Studies on the larval hemocytes in the cabbage with butterfly, Pieris rapae crucivora (Boisduval), with special reference to hemocyte classifications, phagocytic activity and encapsulative capacity. Kontyú. 39:385-394.

[31] Takeda, N. 1977. Brain hormone carrier haemocytes in the moth Monema flavescens. Jour. Insect Physiol. 23:1245-1254.

[32] Toledo, L.A. & E.A. Gregório. 1983. Ultraestrutura dos hemócitos de larvas de Diatraea saccharalis (Lepidoptera: Pyralidae). Resumos do III Congresso Brasileiro de Biologia Celular, São Paulo, p. 47.

[33] Vieira, S. & R. Hoffmann. 1988. Elementos de Estatística. São Paulo, Editora Atlas, 159p.

[34] Wigglesworth, V.B. 1959. Insect blood cells. Ann. Rev. Entomol. 4:1-16.

[35] ______. 1972. Haemocytes and basement membrane formation in Rhodnius. Jour. Insect Physiol. 19:831-844.

[36] ______. 1979a. Hemocytes and growth in insects, p. 303-318. In: A.P. Gupta (Ed.). Insect hemocytes: development, forms, functions and techniques. Cambridge, Cambridge University Press, 614p.

[37] ______. 1979b. Secretory activities of plasmatocytes and oenocytoids during the moulting cycle in an insect (Rhodnius). Tissue Cell. 11:69-78.

Brasil

Brasil

Estudo morfológico e quantitativo dos hemócitos em larvas de Diatraea saccharalis (Fabricius) (Lepidoptera, Pyralidae)

Morphological and quantitative studies of the hemocytes in larvae of Diatraea saccharalis (Fabricius) (Lepidoptera, Pyralidae)

Resumo

Datas de Publicação

Histórico