Evaluation of the haematological parameters in Piaractus mesopotamicus Holmberg (Osteichthyes, Characidae) with Argulus sp. (Crustacea, Branchiura) infestation and treatment with organophosphate

Tavares-Dias, Marcos; Martins, Maurício Laterça; Kronka, Sérgio do Nascimento

doi:10.1590/S0101-81751999000200019

Abstract

Haematological parameters as erythrocytes, leukocytes and plasma glucose in Piaractus mesopotamicus Holmberg, 1887 were analyzed. Fish were parasitized with Argulus sp. (Crustacea, Branchiura) and treated with 0.4 mg of thriclorphon 500/L water. The effects of parasitism and the action of the treatment were evaluated. Parasitized fish showed greater number of monocytes (P>0.05) and special granulocitic cells (P<0.01) in the peripheric blood than the unparasitized (control) fish. Parasitized fish showed lower number of thrombocytes (P>0.05). Organophosphate treatment presented significantly reduction (P>0.05) in red blood cells (RBC) and hemoglobin.

Piaractus mesopotamicus; Argulus; parasitism; haemathology

SCIENTIFIC COMMUNICATION

Evaluation of the haematological parameters in Piaractus mesopotamicus Holmberg (Osteichthyes, Characidae) with Argulus sp. (Crustacea, Branchiura) infestation and treatment with organophosphate

Marcos Tavares-Dias^I; Maurício Laterça Martins^I; Sérgio do Nascimento Kronka^II

^ILaboratório de Patologia de Organismos Aquáticos, Centro de Aqüicultura, Universidade Estadual Paulista. Rodovia Carlos Tonanni Km 05, 14870-000 Jaboticabal, São Paulo, Brasil

^IIDepartamento de Ciências Exatas, Universidade Estadual Paulista. Rodovia Carlos Tonanni Km 05, 14870-000 Jaboticabal, São Paulo, Brasil. Pesquisador do CNPq

ABSTRACT

Haematological parameters as erythrocytes, leukocytes and plasma glucose in Piaractus mesopotamicus Holmberg, 1887 were analyzed. Fish were parasitized with Argulus sp. (Crustacea, Branchiura) and treated with 0.4 mg of thriclorphon 500/L water. The effects of parasitism and the action of the treatment were evaluated. Parasitized fish showed greater number of monocytes (P>0.05) and special granulocitic cells (P<0.01) in the peripheric blood than the unparasitized (control) fish. Parasitized fish showed lower number of thrombocytes (P>0.05). Organophosphate treatment presented significantly reduction (P>0.05) in red blood cells (RBC) and hemoglobin.

Key words:Piaractus mesopotamicus, Argulus, parasitism, haemathology

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ACKNOWLEDGMENTS. We would like to thank MSc. Paulo C.F. Carneiro (Centro de Aqüicultura, UNESP, Jaboticabal) for the correction of the translation of the manuscript.

Recebido em 19.XII.1997; aceito em 29.III.1999.

Collier, H.B. 1944. The standardization of blood haemoglobin determinations. Can. Med. Ass. Jour. 50:550-552.
Goldenfarb, P.B.; F.P. Bowyer; Hall & E. Brosious. 1971. Reproducibility in the hematology laboratory: the microhematocrit determination. Amer. Jour. Clin. Path. 56:35-39.
Pimentel Gomes, F. 1985. Curso de estatística experimental. Piracicaba, Livraria Nobel, 11ª ed., 466p.
Ranzani-Paiva, M.J.; A.L. Vieira & C.M. Ishikawa. 1989. Análise dos constituintes do plasma sangüíneo da carpa comum Cyprinus carpio, infestada por Argulus sp. B. Inst. Pesca, São Paulo, 16:117-121.
Ranzani-Paiva, M.J.; CM. Ishikawa; B.E.S. Campos & A.C das Eiras. 1997. Haematological characteristics associated with parasitism in mullets, Mugil platanus Günther, from the estuarine region of Cananéia, São Paulo, Brazil. Revta bras. Zool. 14 (2):329-339.
Ranzani-Paiva, M.J.; C.M. Ishikawa; M.C Portella & R.J. Celiberto. 1987. Hematologia da carpa comum Cyprinus carpio, infestada por Argulus sp. e após um tratamento com fosfato de 0,0-dimetil-oxi-2,2,2,-tricloroetilo (Neguvon). B. Inst. Pesca, São Paulo, 14:83-92.
Rosenfeld, G. 1947. Corante pancrômico para hematologia e citología clínica. Nova combinação dos componentes do May-Grunwald e do Giensa num só corante de emprego rápido. Mem. Inst. Butantan 20:329-334.
Shimura, S.; K. Inoue; K. Kasai & H. Saito. 1983. Hematological changes of Oncohynchus masou (Salmonidae) caused by the infection of Argulus coregoni (Crustacea: Branchiura). Fish Pathol. Univ. Tokyo 18 (3):157-162.
Wintrobe, M.M. 1934. Variations on the size and hemoglobin content of erythrocytes in the blood various vertebrates. Folia Haematologica. 51:32-49.

Publication Dates

Publication in this collection
01 July 2009
Date of issue
June 1999

History

Accepted
29 Mar 1999
Received
19 Dec 1997

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] Collier, H.B. 1944. The standardization of blood haemoglobin determinations. Can. Med. Ass. Jour. 50:550-552.

[2] Goldenfarb, P.B.; F.P. Bowyer; Hall & E. Brosious. 1971. Reproducibility in the hematology laboratory: the microhematocrit determination. Amer. Jour. Clin. Path. 56:35-39.

[3] Pimentel Gomes, F. 1985. Curso de estatística experimental. Piracicaba, Livraria Nobel, 11ª ed., 466p.

[4] Ranzani-Paiva, M.J.; A.L. Vieira & C.M. Ishikawa. 1989. Análise dos constituintes do plasma sangüíneo da carpa comum Cyprinus carpio, infestada por Argulus sp. B. Inst. Pesca, São Paulo, 16:117-121.

[5] Ranzani-Paiva, M.J.; CM. Ishikawa; B.E.S. Campos & A.C das Eiras. 1997. Haematological characteristics associated with parasitism in mullets, Mugil platanus Günther, from the estuarine region of Cananéia, São Paulo, Brazil. Revta bras. Zool. 14 (2):329-339.

[6] Ranzani-Paiva, M.J.; C.M. Ishikawa; M.C Portella & R.J. Celiberto. 1987. Hematologia da carpa comum Cyprinus carpio, infestada por Argulus sp. e após um tratamento com fosfato de 0,0-dimetil-oxi-2,2,2,-tricloroetilo (Neguvon). B. Inst. Pesca, São Paulo, 14:83-92.

[7] Rosenfeld, G. 1947. Corante pancrômico para hematologia e citología clínica. Nova combinação dos componentes do May-Grunwald e do Giensa num só corante de emprego rápido. Mem. Inst. Butantan 20:329-334.

[8] Shimura, S.; K. Inoue; K. Kasai & H. Saito. 1983. Hematological changes of Oncohynchus masou (Salmonidae) caused by the infection of Argulus coregoni (Crustacea: Branchiura). Fish Pathol. Univ. Tokyo 18 (3):157-162.

[9] Wintrobe, M.M. 1934. Variations on the size and hemoglobin content of erythrocytes in the blood various vertebrates. Folia Haematologica. 51:32-49.