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Revista Brasileira de Zoologia

Print version ISSN 0101-8175

Rev. Bras. Zool. vol.16 no.4 Curitiba  1999

http://dx.doi.org/10.1590/S0101-81751999000400019 

Mapping of serotonin-immunoreactive neurons of Anastrepha obliqua Macquart larvae

 

 

Isabel Cristina BoleliI; Zilá Luz Paulino-SimõesII

IDepartamento de Morfologia e Fisiologia Animal, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista. Rodovia Paulo Donato Castellani Km 5, 14870-000 Jaboticabal, São Paulo, Brasil
IIDepartamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo. Rodovia Bandeirantes 3900, 14040-901 Ribeirão Preto, São Paulo, Brasil

 

 


ABSTRACT

Serotonin-immunoreactive neurons were identified in the central nervous system (CNS) of Anastrepha obliqua Macquart, 1835 wandering stage larvae. The PAP immunocytochemical method was applied to the entire CNS (whole mounts). About 90 neurons were visualized in the CNS (20 in the brain and 70 in the ventral ganglion). Both somata and axons were strongly stained. These neurons showed a segmental arrangement and bilateral symmetry. All processes presented a basic projection pattern, in which the major fibres travel contralaterally. Comparison of these neurons with serotonergic neurons described in other insects suggests order-specific traits such as cerebral clusters and presence of only one 5-HT immunoreactive neuron in the 8th abdominal neuromere as well.

Key words: Anastrepha obliqua, fruit fly, serotonin, immunocytochemistry


 

 

Full text available only in PDF format.

 

Texto completo disponível apenas em PDF.

 

 

Acknowledgements. We thank J. Lauder (University of North Carolina, Chapell Hill) for the kind donation of rabbit antiserotonin serum. We are also grateful to Brazilian Government (CNPq) for financial support for ICB.

 

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Recebido em 18.VI.1998; aceito em 07.X.1999.

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