Desenvolvimento biológico de Angiostrongylus vasorum (Baillet) Kamensnky (Nematoda, Angiostrongylidae) em Subulina octona Bruguière (Molusca, Subulinidae) em condições de laboratório

Bessa, Elisabeth Cristina de Almeida; Lima, Walter dos Santos; Daemon, Erik; Cury, Márcia Cristina; Araújo, José Luiz de Barros

doi:10.1590/S0101-81752000000100004

Resumo

With the purpose of investigating the biological aspects of Angiostrongylus vasorum (Baillet, 1866) Kamensky, 1905 in Subulina octona Bruguiere, 1789 experimental infections were carried out with the exposure of 90 adult molluscs to 155 L1 of the parasite/mollusc during 24 hours. The intramollus-can larval development was checked out daily through the chemical digestion of four infected molluscs and one molluscs from the control group during 21 days after infection. Until the 6th day post-infection, only Li of the parasite has been observed. On the 7th day; the presence of L2 was detected and from the 13th day on, L3 of A. vasorum also appeared. By means of larval morphometry, it has been observed that, Li had na average size of 335 x 14,5 um whereas L2 had an average size of 440,7 x 33,9 um on the 7th day ofter infection, reaching a, aximal diameter of 41,8 um on the 12th day after infections. The L3 had an average size of 472,4 x 38,7 um on the 13th day day and on the 21st' day after infection the measure was 551,3 x 27,6 um. In order to verify the infectivity of L3, from 5. octona to the definitive host, infection of a dog was undertaken by means of ingestion of tritured molluscs. On the 49th day post-infection, Li was found in the feaces of the dog and at necropsy on the 84th day post-infection adults worms were found in the lungs.

Angiostrongylus vasorum; Subulina octona; intra-mollusc larvae evolution

Desenvolvimento biológico de Angiostrongylus vasorum (Baillet) Kamensnky (Nematoda, Angiostrongylidae) em Subulina octona Bruguière (Molusca, Subulinidae) em condições de laboratório

Biological development of Angiostrongylus vasorum (Baillet) Ka-mensky (Nematoda, Metastrongylidae) in Subulina octona Bruguiere (Mollusca, Subulinidae) in laboratory conditions

Elisabeth Cristina de Almeida Bessa^I; Walter dos Santos Lima^II; Erik Daemon^III; Márcia Cristina Cury^II; José Luiz de Barros Araújo^IV

^IDepartamento de Zoologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora. 36036-330 Juiz de Fora, Minas Gerais, Brasil

^IIDepartamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais. 31270-901 Belo Horizonte, Minas Gerais, Brasil

^IIIDepartamento de Parasitologia Animal, Instituto de Biologia, Universidade Federal Rural do Rio de Janeiro. 23890-000 Seropédica, Rio de Janeiro, Brasil

^IVDepartamento de Parasitologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás. 74605-050 Goiânia, Gioás, Brasil

ABSTRACT

With the purpose of investigating the biological aspects of Angiostrongylus vasorum (Baillet, 1866) Kamensky, 1905 in Subulina octona Bruguiere, 1789 experimental infections were carried out with the exposure of 90 adult molluscs to 155 _L1 of the parasite/mollusc during 24 hours. The intramollus-can larval development was checked out daily through the chemical digestion of four infected molluscs and one molluscs from the control group during 21 days after infection. Until the 6^th day post-infection, only Li of the parasite has been observed. On the 7^th day; the presence of L₂ was detected and from the 13^th day on, L₃ of A. vasorum also appeared. By means of larval morphometry, it has been observed that, Li had na average size of 335 x 14,5 um whereas L₂ had an average size of 440,7 x 33,9 um on the 7^th day ofter infection, reaching a, aximal diameter of 41,8 um on the 12^th day after infections. The L₃ had an average size of 472,4 x 38,7 um on the 13^thday day and on the 21^st' day after infection the measure was 551,3 x 27,6 um. In order to verify the infectivity of L₃, from 5. octona to the definitive host, infection of a dog was undertaken by means of ingestion of tritured molluscs. On the 49^th day post-infection, Li was found in the feaces of the dog and at necropsy on the 84^th day post-infection adults worms were found in the lungs.

Key words:Angiostrongylus vasorum, Subulina octona, intra-mollusc larvae evolution

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

Recebido em 07.V.1998: aceito em 01 .II.2000.

Bessa, E.C. de A. & J.L. de B. Araújo. 1995a. Ocorrência de autofecundação em Subulina octona (Burguière) (Pulmanata, Subulinidae) sob condições de laboratório. Revta bras. Zool. 2 (3): 719-723.
______. 1995b. Oviposição, tamanho de ovos e medida do comprimento da concha em diferentes fases da desenvolvimento de Subulina octona (Burguière) (Pulmonata, Subulinidae) em condições de labaratório Revta bras. zool. 2 (3): 647-654.
Cobb, M.A. & M.A. Fisher. 1990. Angiostrongylus vasorum: transmission in south east England Vet. Rec. 126: 529.
Dorchies, P. 1976. Etude zoolique, d' Angiostrongylus vasorum. L'Animal de Compagnie 11: 45-45.
Eckert, J. & G. Lammler. 1972. Angiostrongylose bei mensch und tier. Z. Parasitenkd. 39: 303-322.
Giovannoni, M.; B.F. Fernandez & L.C. KAVINSKI. 1985. Angiostrangilose do cão. Arq. Biol. Tecn., Curitiba, 28: 601-604.
Gonçalves, P.C. 1961. Angiostrongylus vasorum (Baillet, 1866), novo parasito do cão no Rio Grande da Sul (Brasil) (Nematoda: Metastrongyloidea). Rev. Fac. Agron. Vet. UFRGS, Porto Alegre, 4: 35-40.
Guilhon, J. 1960. Role des limacidés dans le cyde evólutif d' Angiostrongylus vasorum (Baillet, 1866). CR. Acad. Sc, Paris, 251: 2252-2253.
Guilhon, J. & A. Afchahi. 1969. Evolution larvaire d' Angiostrongylus vasorum (Baillet, 1866) dans l'organisme de diverses espéces de mollusques terrestres. CR. Acad. Sci, Paris, 268: 434-436.
Guilhon, J. & B. CENS. 1973. Angiostrongylus vasorum (Baillet, 1866) Etude biologique et morphologique. Ann. Parasit. Hum. Comp. 48: 567-596.
Guilhon, J. & A. de Gaalon. 1969. Evolution larvaire d'un nématode parasite de l'appariel circulatoire du chien dans l'organisme de mollusques dulçaquicoles. CR. Acad. Sci, Paris, 268: 612-615.
Langenegger, J.; A.M. Langenegger; P. Dacorso Filho & G.L.A. Gouvea. 1962. Ocorrência da infestação por Angiostrongylus vasorum em cães do Rio de Janeiro. Anais VIII Cong. Brasil. Vet., Belo Horizonte: 246-247.
Lima, W.S.; H.M.A. Costa; M.P. Guimarães & CR. Leite. 1985. Angiostrongylus vasorum (Baillet, 1866) (Nematoda Protostrongylidae) em cães de Minas Gerais, Brasil. Mem. Inst. Oswaldo Cruz 80: 233-235.
Martin, M.W.S.; G. Ashton; V.R. Simpson & C. Neal. 1993. Angiostrongylosis in Corhwall: Clinical presentation of eight cases. Jour. Small Anim. Practice 34: 20-25.
Rachford, F.W. 1976. Host-parasite relationship of Angiostrongylus canionensis in Lymnaeapalustris I. Intramolluscan Larval Grawth and Development. Exp. Parasit. 39: 377-381.
Roberts, F.H.S. 1940, Notes an some helminths infesting domestic animals in Queensland. Austral. Vet. Jour. 16: 30-33.
Rosen, L.; L.A. Ash & G.D. Wallace. 1970. Life history of the canine lungworn Angiostrongylus vasorum (Baillet). Amer. Jour. Vet. Res. 31: 131-143.
Sanchez, A.C.; J.B. Diez; J.G. Morales; L.C. Menendes; J.A.C. Hernandes & J.A.G. Jalon. 1979. Angiostrongylosis canina. Ver. Iber. Parasit. L. 39: 135-142.
Simpson, V.R. & C. Neal. 1982. Angiostrongylus vasorum infection in dogs and slugs. Vet. Ree. III: 303-304.
Ubelaker, L.E. 1986. Systematics of especies refered to the genus Angiostrongylus. Jour. Parasit. 72: 237-244.
Wallace, G.D. & L. Rosen. 1969. Techniques for recovering and identifying larve Angiostrongylus cantonensis from molluscs. Malacologia 7: 427-438.

Datas de Publicação

Publicação nesta coleção
22 Maio 2009
Data do Fascículo
Mar 2000

Histórico

Aceito
01 Fev 2000
Recebido
07 Maio 1998

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] Bessa, E.C. de A. & J.L. de B. Araújo. 1995a. Ocorrência de autofecundação em Subulina octona (Burguière) (Pulmanata, Subulinidae) sob condições de laboratório. Revta bras. Zool. 2 (3): 719-723.

[2] ______. 1995b. Oviposição, tamanho de ovos e medida do comprimento da concha em diferentes fases da desenvolvimento de Subulina octona (Burguière) (Pulmonata, Subulinidae) em condições de labaratório Revta bras. zool. 2 (3): 647-654.

[3] Cobb, M.A. & M.A. Fisher. 1990. Angiostrongylus vasorum: transmission in south east England Vet. Rec. 126: 529.

[4] Dorchies, P. 1976. Etude zoolique, d' Angiostrongylus vasorum. L'Animal de Compagnie 11: 45-45.

[5] Eckert, J. & G. Lammler. 1972. Angiostrongylose bei mensch und tier. Z. Parasitenkd. 39: 303-322.

[6] Giovannoni, M.; B.F. Fernandez & L.C. KAVINSKI. 1985. Angiostrangilose do cão. Arq. Biol. Tecn., Curitiba, 28: 601-604.

[7] Gonçalves, P.C. 1961. Angiostrongylus vasorum (Baillet, 1866), novo parasito do cão no Rio Grande da Sul (Brasil) (Nematoda: Metastrongyloidea). Rev. Fac. Agron. Vet. UFRGS, Porto Alegre, 4: 35-40.

[8] Guilhon, J. 1960. Role des limacidés dans le cyde evólutif d' Angiostrongylus vasorum (Baillet, 1866). CR. Acad. Sc, Paris, 251: 2252-2253.

[9] Guilhon, J. & A. Afchahi. 1969. Evolution larvaire d' Angiostrongylus vasorum (Baillet, 1866) dans l'organisme de diverses espéces de mollusques terrestres. CR. Acad. Sci, Paris, 268: 434-436.

[10] Guilhon, J. & B. CENS. 1973. Angiostrongylus vasorum (Baillet, 1866) Etude biologique et morphologique. Ann. Parasit. Hum. Comp. 48: 567-596.

[11] Guilhon, J. & A. de Gaalon. 1969. Evolution larvaire d'un nématode parasite de l'appariel circulatoire du chien dans l'organisme de mollusques dulçaquicoles. CR. Acad. Sci, Paris, 268: 612-615.

[12] Langenegger, J.; A.M. Langenegger; P. Dacorso Filho & G.L.A. Gouvea. 1962. Ocorrência da infestação por Angiostrongylus vasorum em cães do Rio de Janeiro. Anais VIII Cong. Brasil. Vet., Belo Horizonte: 246-247.

[13] Lima, W.S.; H.M.A. Costa; M.P. Guimarães & CR. Leite. 1985. Angiostrongylus vasorum (Baillet, 1866) (Nematoda Protostrongylidae) em cães de Minas Gerais, Brasil. Mem. Inst. Oswaldo Cruz 80: 233-235.

[14] Martin, M.W.S.; G. Ashton; V.R. Simpson & C. Neal. 1993. Angiostrongylosis in Corhwall: Clinical presentation of eight cases. Jour. Small Anim. Practice 34: 20-25.

[15] Rachford, F.W. 1976. Host-parasite relationship of Angiostrongylus canionensis in Lymnaeapalustris I. Intramolluscan Larval Grawth and Development. Exp. Parasit. 39: 377-381.

[16] Roberts, F.H.S. 1940, Notes an some helminths infesting domestic animals in Queensland. Austral. Vet. Jour. 16: 30-33.

[17] Rosen, L.; L.A. Ash & G.D. Wallace. 1970. Life history of the canine lungworn Angiostrongylus vasorum (Baillet). Amer. Jour. Vet. Res. 31: 131-143.

[18] Sanchez, A.C.; J.B. Diez; J.G. Morales; L.C. Menendes; J.A.C. Hernandes & J.A.G. Jalon. 1979. Angiostrongylosis canina. Ver. Iber. Parasit. L. 39: 135-142.

[19] Simpson, V.R. & C. Neal. 1982. Angiostrongylus vasorum infection in dogs and slugs. Vet. Ree. III: 303-304.

[20] Ubelaker, L.E. 1986. Systematics of especies refered to the genus Angiostrongylus. Jour. Parasit. 72: 237-244.

[21] Wallace, G.D. & L. Rosen. 1969. Techniques for recovering and identifying larve Angiostrongylus cantonensis from molluscs. Malacologia 7: 427-438.

Brasil

Brasil

Desenvolvimento biológico de Angiostrongylus vasorum (Baillet) Kamensnky (Nematoda, Angiostrongylidae) em Subulina octona Bruguière (Molusca, Subulinidae) em condições de laboratório

Biological development of Angiostrongylus vasorum (Baillet) Ka-mensky (Nematoda, Metastrongylidae) in Subulina octona Bruguiere (Mollusca, Subulinidae) in laboratory conditions

Resumo

Datas de Publicação

Histórico