Abstract
The objective of this research is to present a method to bioassay entomopathogenic Bacillus species against sand fly (Diptera, Psychodidae, Phlebotominae) larvae. The vector Lutzomyia longipalpis (Lutz & Neiva, 1912) (Diptera, Psychodidae, Phlebotominae) and some Bacillus strains were used in laboratory to develop this method. Some characteristics of this method are commented and the importance to develop a standard method to test pathogenicity of Bacillus species against phlebotomine larvae is also discussed.
Phlebotominae; Bacillus; bioassay; method
SCIENTIFIC COMMUNICATION
Methodology for bioassay entomopathogenic Bacillus against sand fly (Diptera, Psychodidae, Phlebotominae) larvae
Eduardo Dias WermelingerI,II; José Cola ZanuncioII; Elizabeth F. RangelI; Paulo R. CeconIII; Leon RabinovitchIV
IDepartamento de Entomologia, Instituto Oswaldo Cruz. 21045-900 Rio de Janeiro, Rio de Janeiro, Brasil
IIDepartamento de Biologia Animal, Universidade Federal de Viçosa. 36571-000 Viçosa, Minas Gerais, Brasil. E-mail: zanuncio@mail.ufv.br
IIIDepartamento de Informática, Universidade Federal de Viçosa. 36571-000 Viçosa, Minas Gerais, Brasil
IVDepartamento de Bacteriologia, Instituto Osvaldo Cruz. Caixa Postal 926, 21045-900 Rio de Janeiro, Rio de Janeiro, Brasil
ABSTRACT
The objective of this research is to present a method to bioassay entomopathogenic Bacillus species against sand fly (Diptera, Psychodidae, Phlebotominae) larvae. The vector Lutzomyia longipalpis (Lutz & Neiva, 1912) (Diptera, Psychodidae, Phlebotominae) and some Bacillus strains were used in laboratory to develop this method. Some characteristics of this method are commented and the importance to develop a standard method to test pathogenicity of Bacillus species against phlebotomine larvae is also discussed.
Key words: Phlebotominae, Bacillus, bioassay. method
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ACKNOWLEDGEMENTS. To the staff of the Laboratory of Bacterial Physiology of the Department of Bacteriology and to the Sandfly Insectary, of the Leishmaniasis and Onchocerciasis Vector Laboratory, of the Department of Entomology of Oswaldo Cruz Institute for technical support. To CNPq and FAPEMIG for support.
REFERENCES
Barjac, H. de; L. Larget & R. Killick-Kendrick. 1981. Toxicité de Bacillus thuringiensis var. israelensis, sérotype H14, pour les larves de phlébotomes, vecteurs de leishmanioses. Bull. Soc. Pathol. Exot. 74:485-489.
Oliveira Filho, A.M. & M.T. Melo. 1994. The chemical control of vectors of leishmaniasis. Mem. Inst. Oswaldo Cruz 89:461-462.
Pener, H. & A. Wilamowski. 1996. Susceptibility of larvae of the sandfly Phlebotomus papatasi (Diptera: Psychodidae) to Bacillus sphaericus. Bull. Entomol. Res. 86:173-175.
Rangel, E.F.; N.A. Souza; E.D. Wermelinger & A.F. Barbosa. 1985. Estabelecimento de colônia, em laboratório de Lutzomyia intermedia (Lutz & Neiva, 1912) (Diptera, Psychodidae, Phlebotominae). Mem. Inst. Oswaldo Cruz 80:219-226.
Robert, L.L.; M.J. Perich; Y. Schlein; R.L. Jacobson; R.A. Wirtz; P.G. Lawyer & J.I. Githure. 1997. Phlebolomine sand fly control using bait-fed adults to carry the larvicide Bacillus sphaericus to the larval habitat. Jour. Amer. Mosq. Control Assoc. 13:140-144.
Warburg, A. 1991. Entomopathogens of phlebotomine sand flies: laboratory experiments and natural infections. Jour. Inv. Pathol. 58:189-202.
Warburg, A; K. Ostrovska; P.G. Lawyer. 1991. Pathogens of phlebotomine sandflies. A review. Parassitologia 33(Suppl. 1):519-526.
Wermelinger, E.D.; J.C. Zanuncio; E.F. Rangel & L. Rabinovitch. 1995a. Toxic activity (LC50) of Bacillus thuringiensis subsp. israelensis strain LFB/FIOCRUZ 710 on Lutzomyia longipalpis (Diptera: Psychodidae: Phlebotominar). Amer. Jour. Trop. Med. Hyg. 53(2):163.
______. 1995b. Observations about symptoms and signs on Lutzomyia longipalpis larvae (Lutz & Neiva, 1912) (Diptera; Psychodidae, Phlebotominae) under bioassays with Bacillus thuringiensis subsp. israelensis strains LFB/FIOCRUZ 584 and LFB/FIOCRUZ 710. Mem. Inst. Oswaldo Cruz 90:235.
Wermelinger, E.D.; J.C. Zanuncio; E.F. Rangel; P.R. Cecon & L. Rabinovitch. (in press). Toxicity of Bacillus species to larvae of Lutzomyia longipalpis (L. & N.) (Diptera: Psychodidae: Phlebotominae) Anais Soc. Bras. Entomol. .
Recebido em 19.VIII.1999; aceito em 25.VIII.2000.
- Barjac, H. de; L. Larget & R. Killick-Kendrick. 1981. Toxicité de Bacillus thuringiensis var. israelensis, sérotype H14, pour les larves de phlébotomes, vecteurs de leishmanioses. Bull. Soc. Pathol. Exot. 74:485-489.
- Oliveira Filho, A.M. & M.T. Melo. 1994. The chemical control of vectors of leishmaniasis. Mem. Inst. Oswaldo Cruz 89:461-462.
- Pener, H. & A. Wilamowski. 1996. Susceptibility of larvae of the sandfly Phlebotomus papatasi (Diptera: Psychodidae) to Bacillus sphaericus. Bull. Entomol. Res. 86:173-175.
- Rangel, E.F.; N.A. Souza; E.D. Wermelinger & A.F. Barbosa. 1985. Estabelecimento de colônia, em laboratório de Lutzomyia intermedia (Lutz & Neiva, 1912) (Diptera, Psychodidae, Phlebotominae). Mem. Inst. Oswaldo Cruz 80:219-226.
- Robert, L.L.; M.J. Perich; Y. Schlein; R.L. Jacobson; R.A. Wirtz; P.G. Lawyer & J.I. Githure. 1997. Phlebolomine sand fly control using bait-fed adults to carry the larvicide Bacillus sphaericus to the larval habitat. Jour. Amer. Mosq. Control Assoc. 13:140-144.
- Warburg, A. 1991. Entomopathogens of phlebotomine sand flies: laboratory experiments and natural infections. Jour. Inv. Pathol. 58:189-202.
- Warburg, A; K. Ostrovska; P.G. Lawyer. 1991. Pathogens of phlebotomine sandflies. A review. Parassitologia 33(Suppl. 1):519-526.
- Wermelinger, E.D.; J.C. Zanuncio; E.F. Rangel & L. Rabinovitch. 1995a. Toxic activity (LC50) of Bacillus thuringiensis subsp. israelensis strain LFB/FIOCRUZ 710 on Lutzomyia longipalpis (Diptera: Psychodidae: Phlebotominar). Amer. Jour. Trop. Med. Hyg. 53(2):163.
- ______. 1995b. Observations about symptoms and signs on Lutzomyia longipalpis larvae (Lutz & Neiva, 1912) (Diptera; Psychodidae, Phlebotominae) under bioassays with Bacillus thuringiensis subsp. israelensis strains LFB/FIOCRUZ 584 and LFB/FIOCRUZ 710. Mem. Inst. Oswaldo Cruz 90:235.
- Wermelinger, E.D.; J.C. Zanuncio; E.F. Rangel; P.R. Cecon & L. Rabinovitch. (in press). Toxicity of Bacillus species to larvae of Lutzomyia longipalpis (L. & N.) (Diptera: Psychodidae: Phlebotominae) Anais Soc. Bras. Entomol.
Publication Dates
-
Publication in this collection
11 May 2009 -
Date of issue
Sept 2000
History
-
Accepted
25 Aug 2000 -
Received
19 Aug 1999