Methodology for bioassay entomopathogenic Bacillus against sand fly (Diptera, Psychodidae, Phlebotominae) larvae

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Methodology for bioassay entomopathogenic Bacillus against sand fly (Diptera, Psychodidae, Phlebotominae) larvae

Eduardo Dias Wermelinger^I,II; José Cola Zanuncio^II; Elizabeth F. Rangel^I; Paulo R. Cecon^III; Leon Rabinovitch^IV

^IDepartamento de Entomologia, Instituto Oswaldo Cruz. 21045-900 Rio de Janeiro, Rio de Janeiro, Brasil

^IIDepartamento de Biologia Animal, Universidade Federal de Viçosa. 36571-000 Viçosa, Minas Gerais, Brasil. E-mail: [e-mail]

^IIIDepartamento de Informática, Universidade Federal de Viçosa. 36571-000 Viçosa, Minas Gerais, Brasil

^IVDepartamento de Bacteriologia, Instituto Osvaldo Cruz. Caixa Postal 926, 21045-900 Rio de Janeiro, Rio de Janeiro, Brasil

ABSTRACT

The objective of this research is to present a method to bioassay entomopathogenic Bacillus species against sand fly (Diptera, Psychodidae, Phlebotominae) larvae. The vector Lutzomyia longipalpis (Lutz & Neiva, 1912) (Diptera, Psychodidae, Phlebotominae) and some Bacillus strains were used in laboratory to develop this method. Some characteristics of this method are commented and the importance to develop a standard method to test pathogenicity of Bacillus species against phlebotomine larvae is also discussed.

Key words: Phlebotominae, Bacillus, bioassay. method

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ACKNOWLEDGEMENTS. To the staff of the Laboratory of Bacterial Physiology of the Department of Bacteriology and to the Sandfly Insectary, of the Leishmaniasis and Onchocerciasis Vector Laboratory, of the Department of Entomology of Oswaldo Cruz Institute for technical support. To CNPq and FAPEMIG for support.

REFERENCES

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Wermelinger, E.D.; J.C. Zanuncio; E.F. Rangel & L. Rabinovitch. 1995a. Toxic activity (LC₅₀) of Bacillus thuringiensis subsp. israelensis strain LFB/FIOCRUZ 710 on Lutzomyia longipalpis (Diptera: Psychodidae: Phlebotominar). Amer. Jour. Trop. Med. Hyg. 53(2):163.

______. 1995b. Observations about symptoms and signs on Lutzomyia longipalpis larvae (Lutz & Neiva, 1912) (Diptera; Psychodidae, Phlebotominae) under bioassays with Bacillus thuringiensis subsp. israelensis strains LFB/FIOCRUZ 584 and LFB/FIOCRUZ 710. Mem. Inst. Oswaldo Cruz 90:235.

Wermelinger, E.D.; J.C. Zanuncio; E.F. Rangel; P.R. Cecon & L. Rabinovitch. (in press). Toxicity of Bacillus species to larvae of Lutzomyia longipalpis (L. & N.) (Diptera: Psychodidae: Phlebotominae) Anais Soc. Bras. Entomol. .

Recebido em 19.VIII.1999; aceito em 25.VIII.2000.

Publication Dates

• Publication in this collection
  11 May 2009
• Date of issue
  Sept 2000

History

• Accepted
  25 Aug 2000
• Received
  19 Aug 1999