Identificação de predadores de Orphulella punctata (de Geer) (Orthoptera, Acrididae) através da serologia

Cassaro-Silva, Marta; Serrão, José Eduardo; Sousa-Silva, Carlos; Marques-Pacheco, Josué

doi:10.1590/S0101-81752001000100007

Resumo

Females of Orphulella punctata (de Geer, 1794) were captured in the field and kept in cages with food and boxes filled with moist soil for oviposition. The eggs, macerated with 0,85% saline solution and centrifugated, were used as the immunizing antigen for obtention of the specific antiserum (AS-O). Rabbits were immunized by the linphonodule injection method with two injections of this antigen, with an interval of 15 days between the 1st and 2nd inoculations. Serological tests were performed using double diffusion in agar gel with homologous and heterologous antigens. Specific serological reactions were obtained 14 days after the 1st inoculation. The antiserum of the 30th day gave 5, 2-4 and 1-4 precipitation lines respectively with eggs, female and male antigen. Such differences can be attributed to the high specificity of AS-O. Positive reaction was observed with arachnids and mirmeleontids captured in the field, indicating the predation on O. punctata.

Insecta; arthropod; grasshopper; antiserum; antigen; biological control; predation

Identificação de predadores de Orphulella punctata (de Geer) (Orthoptera, Acrididae) através da serologia

Serological technique for identifying Orphulella punctata (de Geer) (Orthoptera, Acrididae) predators

Marta Cassaro-Silva^I; José Eduardo Serrão^II; Carlos Sousa-Silva^III; Josué Marques-Pacheco^III

^IInstituto de Humanidades, Universidade de Uberaba. Rua Nenê Sabino 1801, 31550-970 Uberaba, Minas Gerais, Brasil. E-mail: martacass@hotmail.com

^IIDepartamento de Biologia Geral, Universidade Federal de Viçosa. 36571-000 Viçosa, Minas Gerais, Brasil

^IIIDepartamento de Ecologia e Biologia Evolutiva, Universidade Federal de São Carlos. Rodovia Washington Luiz, Km 235, 13560-000 São Carlos, São Paulo, Brasil. E-mail: dcrs@power.ufscar.br

ABSTRACT

Females of Orphulella punctata (de Geer, 1794) were captured in the field and kept in cages with food and boxes filled with moist soil for oviposition. The eggs, macerated with 0,85% saline solution and centrifugated, were used as the immunizing antigen for obtention of the specific antiserum (AS-O). Rabbits were immunized by the linphonodule injection method with two injections of this antigen, with an interval of 15 days between the 1^st and 2^nd inoculations. Serological tests were performed using double diffusion in agar gel with homologous and heterologous antigens. Specific serological reactions were obtained 14 days after the 1^st inoculation. The antiserum of the 30^th day gave 5, 2-4 and 1-4 precipitation lines respectively with eggs, female and male antigen. Such differences can be attributed to the high specificity of AS-O. Positive reaction was observed with arachnids and mirmeleontids captured in the field, indicating the predation on O. punctata.

Key words: Insecta, arthropod, grasshopper, antiserum, antigen, biological control, predation

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AGRADECIMENTOS. Ao CNPq, pelo financiamento da pesquisa. Ao Prof. Dr. Toledo Pizza (

in memorian

) pela identificação da espécie usada no trabalho. Ao Centro de Pesquisa Pecuária do Sudeste-Embrapa, São Carlos, São Paulo, pela permissão para coletas em suas dependências.

Recebido em 30.IX.1999; aceito em 29.III.2001.

Barrera, M. & S.Z. Turk. 1977. Acridios del Noa: Contribución al conocimiento de huevos, desoves y hábitos de postura de algunas especies de tucuras (Orthoptera: Acrididae) de la Provincia de Tucumán. Acta Zool. Lilloana 32(9):167-188.
Dempster, J.P. 1960. A quantitative study of the predators on the eggs and larvae of broom beetle Phytodecla olivaceae Forster, using the precipitation test. Jour. Anim. Ecol. 29:149-167.
Ebert, J.D. 1970. Mecanismos do Desenvolvimento. São Paulo. Livraria Pioneira, Edusp, 274p.
Figueiredo, M.B.; A.P.C. Alba & A.R. Oliveira. 1977. Sorologia aplicada ao estudo de fungos fitopatológicos. Summa Phytopathol. 3:233-259.
Fox, C.J.S. & C.R. MacLellan. 1956. Some Carabidae and Staphylinidae shown to feed on a wireworm, Agriotes sputator (L.), by the precipitin test. Can. Entomol. 88:228-231.
Gorayeb, I.S. & R.P. Pinger. 1978. Detecção de predadores naturais das larvas de Simulium fulvinotum, Cerq. e Mello, 1968 (Diptera: Nematocera). Acta Amazon. 8(4):629-637.
Greenstone, M.H. 1989. Foreign exploration for predators: a proposed new methodology. Environm. Entomol. 18(2):195-200.
Joern, A. & T.N. Rudd. 1982. Impact of predation by the robber fly Proctacanthus milbertii (Diptera: Asilidae) on grasshopper (Orthoptera: Acrididae) populations. Oecologia 55(1):42-46.
Mollet, J. A. & E.J. Armbrust. 1977. Age specific serological identification of adult stages of alfalfa weevil, Hypera postica. Ann. Entomol. Soc. Am. 71(2):207-211.
Mukerji, M.K. & A.B. Ewen. 1984. Field evaluation of cypermethrin and carbaryl as sprays and baits for grasshopper (Orthoptera: Acrididae) control in Saskatchewan. Can. Entomol. 116:5-9.
Oliveira, A.R. 1975. Considerações sobre antissoros obtidos pela técnica de injeção de antígeno no linfonódulo. Summa Phytopathol. 1:61-64.
Oriani, M.A.G.; J.M. Pacheco & CR. Sousa-Silva. 1996. Estudo de herbivoria em Solanum lycocarpum (Solanaceae) através de técnica serológica. Anais VII Semin. Reg. Ecol. 7:121-126.
Otte, D. 1978. Revision of the grasshoppers tribe Orphulellini (Acrididae: Gomphocerinae). Proc. Acad. Nat. Sci. Phil. 131:52-88.
Ouchterlony, O. 1958. Diffusion in gel methods for immunological analysis, p. 1-78. In: S. Karger (Ed.). Progress in allergy. New York, Plenum Press, 312p.
Serrão, J.E.; M. Silva; C.R. Sousa-Silva & J.M. Pacheco. 1997. Uso de serologia na identificação de predadores de Orphulella punctata (de Geer) (Orthoptera: Acrididae). An. Soc. Entomol. Brasil 26(2):375-378.
Silva, A.G.A; CR. Gonçalves; D.M. Monteiro; A.J.L. Gonçalves; J. Gomes; M.N. Silur & L. Simoni. 1968. Quarto catálogo dos insetos que vivem nas plantas do Brasil. Rio de Janeiro, Escola Nac. Agronomia, 622p.
Sousa-Silva, CR.; A.R. Oliveira & J.M. Pacheco. 1988. Diferenciação serológica dos estágios fisiológicos de Deois flavopicta (Stâl, 1854) (Homoptera: Cercopidae). An. Soc. Entomol. Brasil 17:61-65.
______. 1990. Serologia aplicada à determinação de predadores de Deois flavopicta (Stâl, 1854) (Homoptera: Cercopidae). An. Soc. Entomol. Brasil 19:121-125.
Telfer, W.H. 1954. Immunological studies of insect metamorphosis. II. The role of a sex-limited blood protein in egg formation by Cecropia silkworm. Jour. Gen. Physiol. 37:539-558.
Titova, E.V. 1970. Use of precipitin test in a study of interrelationship between Eurygaster intergriceps Put (Hemiptera: Scutelleridae) and predatory arthropods. Entomol. Rev. 49:155-162.
West, A.S. 1950. The precipitin test as an entomological tool. Can. Entomol. 82:241-244.

Datas de Publicação

Publicação nesta coleção
30 Abr 2009
Data do Fascículo
Mar 2001

Histórico

Recebido
30 Set 1999
Aceito
29 Mar 2001

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] Barrera, M. & S.Z. Turk. 1977. Acridios del Noa: Contribución al conocimiento de huevos, desoves y hábitos de postura de algunas especies de tucuras (Orthoptera: Acrididae) de la Provincia de Tucumán. Acta Zool. Lilloana 32(9):167-188.

[2] Dempster, J.P. 1960. A quantitative study of the predators on the eggs and larvae of broom beetle Phytodecla olivaceae Forster, using the precipitation test. Jour. Anim. Ecol. 29:149-167.

[3] Ebert, J.D. 1970. Mecanismos do Desenvolvimento. São Paulo. Livraria Pioneira, Edusp, 274p.

[4] Figueiredo, M.B.; A.P.C. Alba & A.R. Oliveira. 1977. Sorologia aplicada ao estudo de fungos fitopatológicos. Summa Phytopathol. 3:233-259.

[5] Fox, C.J.S. & C.R. MacLellan. 1956. Some Carabidae and Staphylinidae shown to feed on a wireworm, Agriotes sputator (L.), by the precipitin test. Can. Entomol. 88:228-231.

[6] Gorayeb, I.S. & R.P. Pinger. 1978. Detecção de predadores naturais das larvas de Simulium fulvinotum, Cerq. e Mello, 1968 (Diptera: Nematocera). Acta Amazon. 8(4):629-637.

[7] Greenstone, M.H. 1989. Foreign exploration for predators: a proposed new methodology. Environm. Entomol. 18(2):195-200.

[8] Joern, A. & T.N. Rudd. 1982. Impact of predation by the robber fly Proctacanthus milbertii (Diptera: Asilidae) on grasshopper (Orthoptera: Acrididae) populations. Oecologia 55(1):42-46.

[9] Mollet, J. A. & E.J. Armbrust. 1977. Age specific serological identification of adult stages of alfalfa weevil, Hypera postica. Ann. Entomol. Soc. Am. 71(2):207-211.

[10] Mukerji, M.K. & A.B. Ewen. 1984. Field evaluation of cypermethrin and carbaryl as sprays and baits for grasshopper (Orthoptera: Acrididae) control in Saskatchewan. Can. Entomol. 116:5-9.

[11] Oliveira, A.R. 1975. Considerações sobre antissoros obtidos pela técnica de injeção de antígeno no linfonódulo. Summa Phytopathol. 1:61-64.

[12] Oriani, M.A.G.; J.M. Pacheco & CR. Sousa-Silva. 1996. Estudo de herbivoria em Solanum lycocarpum (Solanaceae) através de técnica serológica. Anais VII Semin. Reg. Ecol. 7:121-126.

[13] Otte, D. 1978. Revision of the grasshoppers tribe Orphulellini (Acrididae: Gomphocerinae). Proc. Acad. Nat. Sci. Phil. 131:52-88.

[14] Ouchterlony, O. 1958. Diffusion in gel methods for immunological analysis, p. 1-78. In: S. Karger (Ed.). Progress in allergy. New York, Plenum Press, 312p.

[15] Serrão, J.E.; M. Silva; C.R. Sousa-Silva & J.M. Pacheco. 1997. Uso de serologia na identificação de predadores de Orphulella punctata (de Geer) (Orthoptera: Acrididae). An. Soc. Entomol. Brasil 26(2):375-378.

[16] Silva, A.G.A; CR. Gonçalves; D.M. Monteiro; A.J.L. Gonçalves; J. Gomes; M.N. Silur & L. Simoni. 1968. Quarto catálogo dos insetos que vivem nas plantas do Brasil. Rio de Janeiro, Escola Nac. Agronomia, 622p.

[17] Sousa-Silva, CR.; A.R. Oliveira & J.M. Pacheco. 1988. Diferenciação serológica dos estágios fisiológicos de Deois flavopicta (Stâl, 1854) (Homoptera: Cercopidae). An. Soc. Entomol. Brasil 17:61-65.

[18] ______. 1990. Serologia aplicada à determinação de predadores de Deois flavopicta (Stâl, 1854) (Homoptera: Cercopidae). An. Soc. Entomol. Brasil 19:121-125.

[19] Telfer, W.H. 1954. Immunological studies of insect metamorphosis. II. The role of a sex-limited blood protein in egg formation by Cecropia silkworm. Jour. Gen. Physiol. 37:539-558.

[20] Titova, E.V. 1970. Use of precipitin test in a study of interrelationship between Eurygaster intergriceps Put (Hemiptera: Scutelleridae) and predatory arthropods. Entomol. Rev. 49:155-162.

[21] West, A.S. 1950. The precipitin test as an entomological tool. Can. Entomol. 82:241-244.

Brasil

Brasil

Identificação de predadores de Orphulella punctata (de Geer) (Orthoptera, Acrididae) através da serologia

Serological technique for identifying Orphulella punctata (de Geer) (Orthoptera, Acrididae) predators

Resumo

Datas de Publicação

Histórico