Acessibilidade / Reportar erro
• Rev. Bras. Zool. 18 (2) • Jun 2001 • https://doi.org/10.1590/S0101-81752001000200018   copiar

Polimorfismo enzimático em populações de Melipona quadrifasciata anthidioides Lepeletier (Hymenoptera, Apidae, Meliponinae)

Enzymatic polymorphism in Melipona quadrifasciata anthidioides Lepeletier populations (Hymenoptera, Apidae, Meliponinae)

Autoria SCIMAGO INSTITUTIONS RANKINGS

Resumo

Them aim scope of this study is to characterize the enzymatic polymorphism found in the Melipona quadrifasciata Lepeletier, 1936 populations from Ribeirão Preto, São Paulo and Espírito Santo, Brazil and its hybrids. Samples from each colony (about 52) were prepared for starch gel electrophoresis in order to investigate the genetic variation of the following enzimes: esterase (EST), isocitrate dehydrogenase (IDH), malic enzyme (ME), phosphoglucomutase (PGM), superoxide desmutase (SOD), α-glycerophosphate dehydrogenase (αPGD), malate dehydrogenase (MDH), leucine aminopeptidase (LAP), hexokinase (HK) and phosphoglucose isomerase (PGI). The analysis showed that LAP and HK did not show enzymatic activity and EST showed two alleles(est-sand and est-f) while all the others were shown to be monomorphic. The allele EST-S showed a frequency of 82,6%.

Melipona quadrifasciata anthidioides; polymorphism; electrophoresis; enzyme; stingless bees

Melipona quadrifasciata anthidioides; polymorphism; electrophoresis; enzyme; stingless bees

Polimorfismo enzimático em populações de Melipona quadrifasciata anthidioides Lepeletier (Hymenoptera, Apidae, Meliponinae)

Enzymatic polymorphism in Melipona quadrifasciata anthidioides Lepeletier populations (Hymenoptera, Apidae, Meliponinae)

Davi S. Aidar; Rubiane C. Pagotto; Eucleia P.B. Contel

Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo. Avenida Bandeirantes 3900, Monte Alegre, 14049-900 Ribeirão Preto, São Paulo, Brasil

ABSTRACT

Them aim scope of this study is to characterize the enzymatic polymorphism found in the Melipona quadrifasciata Lepeletier, 1936 populations from Ribeirão Preto, São Paulo and Espírito Santo, Brazil and its hybrids. Samples from each colony (about 52) were prepared for starch gel electrophoresis in order to investigate the genetic variation of the following enzimes: esterase (EST), isocitrate dehydrogenase (IDH), malic enzyme (ME), phosphoglucomutase (PGM), superoxide desmutase (SOD), α-glycerophosphate dehydrogenase (αPGD), malate dehydrogenase (MDH), leucine aminopeptidase (LAP), hexokinase (HK) and phosphoglucose isomerase (PGI). The analysis showed that LAP and HK did not show enzymatic activity and EST showed two alleles(est-sand and est-f) while all the others were shown to be monomorphic. The allele EST-S showed a frequency of 82,6%.

Key words:Melipona quadrifasciata anthidioides, polymorphism, electrophoresis, enzyme, stingless bees

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

AGRADECIMENTOS. Agradecemos ao CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) e FAPEMIG (Fundação de Amparo a Pesquisas de Minas Gerais) pelos recursos doados. Ao Prof. Warwick Estevam Kerr, agradecemos pela leitura do manuscrito e pelas sugestões e pela orientação.

Recebido em 29.VI.2000; aceito em 22.V.2001.

  • AIDAR, D.S. 1996. A Mandaçaia: Biologia de abelhas, manejo e multiplicação artificial de colônias de Melipona quadrifasciata Lep. (Hymenoptera, Apidae, Meliponinae). Brazil. Jour. Genetics, Sér. Monogr., 4:50-58.
  • CABELLO, P.H. & H. KRIEGER. 1991. GENIOC: Programoteca. Rio de Janeiro, Fund. Inst. Oswaldo Cruz.
  • CONTEL, E.P.B. & M.A. MESTRINER. 1974. Esterase polymorphism at two in the social bee. Jour. Heredity 65:349-352.
  • ______. 1974. Esterase polymorphisms at two loci in the social bee. Jour. Hered. 65:349-352.
  • CONTEL, E.P.E. & W.E. KERR. 1976. Origin of males in Melipona subnitida estimated from data of an isozymic polymorphic system. Genetica 46:271-277.
  • FALCÃO, T.M.M.A. & E.P.E. CONTEL. 1990. Genetic variability in natural populations of Brazilian social bees. I. eletrophoretic data for esterases and total proteins. Brazil. Jour. Genet. 13:731-754.
  • ______. 1991a. Genetic variability in natural population of Brazilian social bees. II. Electrophoretic data for PGM and MDH give evidence for multiple fertilization in stingless bees. Rev. Brasil. Genet. 14(1):47-59.
  • ______. 1991b. Genetic variability in natural population of Brazilian social bees. III. Electrophoretic data for ME, GPD, SOD and IDH. Rev. Brasil. Genet. 14(1):61-72.
  • KERR, W.E & W. KRAUSE. 1950. Contribuição para o conhecimento da bionomia dos Meliponini. Fecundação da rainha em Melipona quadrifasciata Lep. Dusenia, Curitiba, 1(15):275-282.
  • LIMA, L.M.K.S. & M.A. MESTRINER. 1985. Starch gel eletrophoretic patterns of esterases and nonspecific proteins in 11 different species of Meliponinae bees. Brazil. Jour. Genet. 8:639-652.
  • PAXTON, R.J.; N. WEIBSHUH; W. ENGLS; K. HARTFELDER & J.J.G. QUEZADA-EUAN. 1999. Not only single mating in stingless bees. Naturwissenschaften 86:143-146.
  • YONG, H.S. 1986. Allozime variation in the stingless bee Trigona fuscobalteata (Hymenoptera, Apidae) from peninsular Malaysia. Comp. Biochem. Physiol. 83(3):627-628.

Datas de Publicação

  • Publicação nesta coleção
    04 Maio 2009
  • Data do Fascículo
    Jun 2001

Histórico

  • Aceito
    22 Maio 2001
  • Recebido
    29 Jun 2000
Creative Common - by-nc 4.0
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Sociedade Brasileira de Zoologia Caixa Postal 19020, 81531-980 Curitiba PR Brasil, Tel./Fax: +55 41 3266-6823, - Curitiba - PR - Brazil
E-mail: sbz@bio.ufpr.br
Acompanhe os números deste periódico no seu leitor de RSS