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Revista Brasileira de Zoologia

Print version ISSN 0101-8175

Rev. Bras. Zool. vol.18 no.3 Curitiba Sept. 2001 

Metodologia de infecção experimental e grau de susceptibilidade do híbrido "tambacu" e Leporinus macrocephalus Garavello & Britski (Osteichthyes, Anostomidae) a quatro inóculos de trofozoítos de Icthyophthirius multifiliis Fouquet (Protozoa, Ciliophora)


Methodology of experimental infection and the susceptibility degree of the tambacu hybrid and Leporinus mucrocephalus Garavello & Britski to four inocula of trophonts of Ichtyophthirius multifiliis Fouquet



Valéria Nogueira de SouzaI; Maurício Laterça MartinsI,III; Flávio Ruas de MoraesI,II,III; Sérgio do Nascimento KronkaIV

ICentro de Aqüicultura, Universidade Estadual Paulista. Rodovia Prof. Paulo Donato Castellani, Km 5, 14870-000 Jaboticabal, São Paulo, Brasil
IIDepartamento de Patologia Veterinária, Universidade Estadual Paulista. Rodovia Prof. Paulo Donato Castellani, Km 5, 14870-000 Jaboticabal, São Paulo, Brasil
IIICentro de Pesquisas em Sanidade Animal, CPPAR-UNESP. Rodovia Prof. Paulo Donato Castellani, km 5, 14870-000 Jaboticabal, São Paulo, Brasil
IVDepartamento de Ciências Exatas, Universidade Estadual Paulista. Rodovia Prof. Paulo Donato Castellani, km 5, 14870-000 Jaboticabal, São Paulo, Brasil





Tropical fishes like Leporinus macrocephalus Garavello & Britski, 1988 ("piauçu") and the hybrid "tambacu" (Piaractus mesopotamicus Holmberg, 1887 "pacu" male x Colossoma macropomum Cuvier, 1818 "tambaqui" female) were experimentally infected with four inocula of trophonts of Ichthyophthirius multifiliis Fouquet, 1876 (Protozoa, Ciliophora) and the infection level was evaluated. Each one of twelve experimental units consisted of a tank with 200 liters of water and two specimens of each kind of fish. The following treatments were designed to each tank, in triplicate: 8, 16,32 and 64 trophonts. After 8 days of exposure the fishes were killed and the number of trophonts was counted at a stereoscopic microscope. This assay showed to be feasible at tropical conditions and was able to show that "tambacu" was more susceptiblc than "piauçu".

Key words: Ichthyophthirius multifiliis, tropical fish, experimental infection, susceptibility



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Flávio Ruas de Moraes

Recebido em 14.XI.2000; aceito em 24.VII.2001.

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