Dynamics and cytochemistry of oogenesis in Leporinus striatus Kner (Teleostei, Characiformes, Anostomidae) from the Rio Sapucaí, Minas Gerais State, Brazil

Chini, Helena A.S.; Garcia, José Antônio Dias; Maistro, Edson Luis; Quagio-Grassiotto, Irani

doi:10.1590/S0101-81752001000400004

Abstract

Oogenesis involves a sequense of transformations which are undergone by female germ cells. These cells change into oogonias and then into mature oocytes. Sexually mature females were collected monthly, during one year, from the Rio Sapucaí, tributary of the Rio Grande, which is part of the Furnas Reservoir system in the state of Minas Gerais. The observed material showed that oogonias were small spherical cells, had a big spherical nucleus, with a single nucleolus, and weakly stained cytoplasm with eosinophilic granules (FG stained), which indicate their protein content. The primary oocytes showed a big basophilic nucleus, with a large peripheral nucleolus, and several smaller nucleoli. They show a reduced cytoplasmic content. The previtellogenic oocytes presented voluminous cytoplasm and nucleus with several small peripheral nucleoli. The oocytes underwent vitellogenesis with the development of the zona radiata and the follicle cells. The zona radiata had two layers, the outer and the inner, which showed its protein content when stained with CM and FG techniques. TB pH 2.5 and pH 4.0 staining showed that oocytes undergoing vitellogenesis presented weakly stained cytoplasm and peripheral cytoplasmic vesicles. The follicle cells that were squamous became cuboidal. In mature oocytes, the yolk granules that filled the cytoplasm became green and blue when stained with FG and CM techniques, indicating their protein content. The perivitclline region showed rosy stained vesicles (TB pH 2.5 and pH 4.0) spread among the weakly stained peripheral vesicles, which seemed to be the cortical alveoli. The zona radiata cells, CM and FG stained, still showed two layers like the oocytes from the previous stage, but thicker.

Teleostei; Anostomidae; Leporinus striatus; oogenesis; cytochemical

Dynamics and cytochemistry of oogenesis in Leporinus striatus Kner (Teleostei, Characiformes, Anostomidae) from the Rio Sapucaí, Minas Gerais State, Brazil

Helena A.S. Chini^I; José Antônio Dias Garcia^II; Edson Luis Maistro^III; Irani Quagio-Grassiotto^IV

^IInstituto de Odontologia e Ciências da Saúde, UNIFENAS. Caixa Postal 23, 37130-000 Alfenas, Minas Gerais, Brasil

^IIFaculdade de Medicina Veterinária, Departamento de Medicina Veterinária Preventiva, UNIFENAS. Caixa Postal 23, 37130-000 Alfenas, Minas Gerais, Brasil

^IIIInstituto de Farmácia e Nutrição, UNIFENAS. Caixa Postal 23, 37130-000 Alfenas, Minas Gerais, Brasil. Brasil. E-mail: maistro.edson@unifenas.br

^IVDepartamento de Morfologia, Instituto de Biociências, Universidade Estadual Paulista. Campus Rubião Júnior, 18300-000 Botucatu, São Paulo, Brasil

ABSTRACT

Oogenesis involves a sequense of transformations which are undergone by female germ cells. These cells change into oogonias and then into mature oocytes. Sexually mature females were collected monthly, during one year, from the Rio Sapucaí, tributary of the Rio Grande, which is part of the Furnas Reservoir system in the state of Minas Gerais. The observed material showed that oogonias were small spherical cells, had a big spherical nucleus, with a single nucleolus, and weakly stained cytoplasm with eosinophilic granules (FG stained), which indicate their protein content. The primary oocytes showed a big basophilic nucleus, with a large peripheral nucleolus, and several smaller nucleoli. They show a reduced cytoplasmic content. The previtellogenic oocytes presented voluminous cytoplasm and nucleus with several small peripheral nucleoli. The oocytes underwent vitellogenesis with the development of the zona radiata and the follicle cells. The zona radiata had two layers, the outer and the inner, which showed its protein content when stained with CM and FG techniques. TB pH 2.5 and pH 4.0 staining showed that oocytes undergoing vitellogenesis presented weakly stained cytoplasm and peripheral cytoplasmic vesicles. The follicle cells that were squamous became cuboidal. In mature oocytes, the yolk granules that filled the cytoplasm became green and blue when stained with FG and CM techniques, indicating their protein content. The perivitclline region showed rosy stained vesicles (TB pH 2.5 and pH 4.0) spread among the weakly stained peripheral vesicles, which seemed to be the cortical alveoli. The zona radiata cells, CM and FG stained, still showed two layers like the oocytes from the previous stage, but thicker.

Key words: Teleostei, Anostomidae, Leporinus striatus, oogenesis, cytochemical

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ACKNOWLEDGEMENTS. The authors are grateful to Mr. Antônio Vicente Salvador and Mr. Ricardo André dos Santos for the technical help, and to Dr. Francisco Langeani Filho for identifying the specimens. Funds supporting this study were provided by FAPEMIG. Hidroelétrica de Furnas and UNIFENAS.

Recebido em 31.VIII.2000; aceito em 15.X.2001.

Anderson, E. 1968. Cortical alveoli formation and vitellogenesis during oocyte differentiation in the pipefish, Syngnathus fuscus and the killifish. Fundulus heteroclitus. Jour. Morphol. 125:23-60.
Bazzoli, N. & E. Rizzo. 1990. A comparative cytological and cytochemical study of the oogenesis in ten Brazilian teleost fish species. Eur. Arch. Biol. 104(4):399-410.
Bazzoli, N & H.P. Godinho. 1994. Cortical alveoli in oocytes of freshwater neotropical teleost fish. Boll. Zool. 61(4):301-308.
______. 1995. Comparative morphology of the yolk nucleus (Balbiani body) in freshwater neotropical teleost fish. Rev. Brasil. Biol. 55:207-214.
______. 1997. Ovócitos vitogênicos do surubim Pseudoplatystoma coruscans e do pacamã Lophiosilurus alexandri. Coleção Meio Ambiente, Belo Horizonte, 19:81-90.
Bazzoli, N.; E. Rizzo: G.B. Santos & J.E.Y. Sato. 1996. Dinâmica da ovogênese em peixes forrageiros da represa de Três Marias, Minas Gerais: estudo histológico e histoquímico. BIOS,Belo Horizonte. 4(4):5-10.
Bristski, H.A. 1972. Peixes de água doce do Estado de São Paulo: Sistemática, p. 79-108. In: S.M. Branco (Ed). Poluição e Piscicultura. São Paulo, Faculdade de Saúde Pública USP, Instituto de Pesca, 216p.
Coello, S.A. 1989. New staining schedule for formalin-fixed, glicol methacrylate- embedded fish ovaries. Jour. Fish Biol. 34:329-330.
de Vlaming, V. 1983. Oocyte development patterns and hormonal involvements among teleost, p. 176-199. In: J. C Rankin; T.J. Pitcher & R.T. Duggan (Eds). Control processes in fish physiology. London, Croom Helm, 287p.
Guraya, S.S. 1986. The cell and molecular biology of fish oogenesis. Basel, H.W. Sauer, 223p.
Hibiya, T. 1983. An atlas of fish histology: normal and pathologic features. Tokyo, Kodasha.
Karnovsky, J.M. 1964. A formaldehyde-glutaraldehyde fixative of high osmolarity for use in Eletron Microscopy. Jour. Cell Biol. 27:137.
Nagahama, Y. 1983. The functional morphology of teleost gonads, p. 223-276. In: W.S. Hoar; D.J. Randall & E.M. Donaldson (Eds). Fish Physiology. New York, Academic Press. Vol. 9, 338p.
Neves, C.A.; D.R. Andrade; S.L.P Matta; M.V. Vidal & A.A. Santos. 1995. Cytochemical analysis of polysaccharides from the cortical alveoli of the oocytes of the lambari-bocarra (Oligosarcurs argenteus Günther, 1864) (Pisces, Characidae). Rev. Brasil. Biol. 55:693-696.
Nomura, H. 1984. Dicionário dos peixes do Brasil. Brasília, Editerra. 482p.
Rizzo, E. & N. Bazzoli. 1993. Oogenesis, oocyte surface and micropylar apparatus of Prochilodus affinus Reinhardt, 1874 (Pisces, Characiformes). Eur. Arch. Biol. 104:1-6.
Selman, K. & R.A. Wallace. 1989. Cellular aspects of oocyte growth in teleosts. Zool. Scien. 6:211-231.
Silva, J.P.D., A.M.P. Filho & N.S.D. Reis. 1998. Caracterização macro e microscópica dos ovários do bagre amarelo. Cathorops spixii (Agassiz, 1829), durante o ciclo reprodutivo. Rev. Brasil. Oceanogr. 46(2):171-185.
Sumpter, J.P.; A.P. Scott; S.M. Baynes & P.R. Winttames. 1984. Early stages of the reproductive cycle in virgin female rainbow trout (Salmo gairdneri Richardson). Aquaculture 43:235-242.
Tyler, C.R. & J.P. Sumpter. 1996. Oocyte growth and development in teleosts. Rev. Fish Biol. Fish. 6:287-318.
Vazzoler, A.E.A.M. 1996. Biologia da reprodução de peixes teleósteos. Teoria e Prática. Maringá, EDUEM, 169p.
Wallace, R.A. & K. Selman. 1981. Celular and dynamic aspects of occyte growth in teleosts. Amer. Zool. 21:325-343.
West, G. 1990. Methods of assessing ovarian development in fish: a review. Aust. Jour. Mar. Freshw. Res. 41(2):199-222.
Yamomoto, K. & H. Yoshioka. 1964. Rhythm of development in the oocyte of medaka, Oryzias latipes. Bull. Fac. Fish. Hokkaido Univ. 15(1):50-19.
Zanuy, S. 1987. Reproduction en aquacultura. Madrid, Caicyt

Publication Dates

Publication in this collection
03 June 2009
Date of issue
Dec 2001

History

Accepted
15 Oct 2001
Received
31 Aug 2000

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] Anderson, E. 1968. Cortical alveoli formation and vitellogenesis during oocyte differentiation in the pipefish, Syngnathus fuscus and the killifish. Fundulus heteroclitus. Jour. Morphol. 125:23-60.

[2] Bazzoli, N. & E. Rizzo. 1990. A comparative cytological and cytochemical study of the oogenesis in ten Brazilian teleost fish species. Eur. Arch. Biol. 104(4):399-410.

[3] Bazzoli, N & H.P. Godinho. 1994. Cortical alveoli in oocytes of freshwater neotropical teleost fish. Boll. Zool. 61(4):301-308.

[4] ______. 1995. Comparative morphology of the yolk nucleus (Balbiani body) in freshwater neotropical teleost fish. Rev. Brasil. Biol. 55:207-214.

[5] ______. 1997. Ovócitos vitogênicos do surubim Pseudoplatystoma coruscans e do pacamã Lophiosilurus alexandri. Coleção Meio Ambiente, Belo Horizonte, 19:81-90.

[6] Bazzoli, N.; E. Rizzo: G.B. Santos & J.E.Y. Sato. 1996. Dinâmica da ovogênese em peixes forrageiros da represa de Três Marias, Minas Gerais: estudo histológico e histoquímico. BIOS,Belo Horizonte. 4(4):5-10.

[7] Bristski, H.A. 1972. Peixes de água doce do Estado de São Paulo: Sistemática, p. 79-108. In: S.M. Branco (Ed). Poluição e Piscicultura. São Paulo, Faculdade de Saúde Pública USP, Instituto de Pesca, 216p.

[8] Coello, S.A. 1989. New staining schedule for formalin-fixed, glicol methacrylate- embedded fish ovaries. Jour. Fish Biol. 34:329-330.

[9] de Vlaming, V. 1983. Oocyte development patterns and hormonal involvements among teleost, p. 176-199. In: J. C Rankin; T.J. Pitcher & R.T. Duggan (Eds). Control processes in fish physiology. London, Croom Helm, 287p.

[10] Guraya, S.S. 1986. The cell and molecular biology of fish oogenesis. Basel, H.W. Sauer, 223p.

[11] Hibiya, T. 1983. An atlas of fish histology: normal and pathologic features. Tokyo, Kodasha.

[12] Karnovsky, J.M. 1964. A formaldehyde-glutaraldehyde fixative of high osmolarity for use in Eletron Microscopy. Jour. Cell Biol. 27:137.

[13] Nagahama, Y. 1983. The functional morphology of teleost gonads, p. 223-276. In: W.S. Hoar; D.J. Randall & E.M. Donaldson (Eds). Fish Physiology. New York, Academic Press. Vol. 9, 338p.

[14] Neves, C.A.; D.R. Andrade; S.L.P Matta; M.V. Vidal & A.A. Santos. 1995. Cytochemical analysis of polysaccharides from the cortical alveoli of the oocytes of the lambari-bocarra (Oligosarcurs argenteus Günther, 1864) (Pisces, Characidae). Rev. Brasil. Biol. 55:693-696.

[15] Nomura, H. 1984. Dicionário dos peixes do Brasil. Brasília, Editerra. 482p.

[16] Rizzo, E. & N. Bazzoli. 1993. Oogenesis, oocyte surface and micropylar apparatus of Prochilodus affinus Reinhardt, 1874 (Pisces, Characiformes). Eur. Arch. Biol. 104:1-6.

[17] Selman, K. & R.A. Wallace. 1989. Cellular aspects of oocyte growth in teleosts. Zool. Scien. 6:211-231.

[18] Silva, J.P.D., A.M.P. Filho & N.S.D. Reis. 1998. Caracterização macro e microscópica dos ovários do bagre amarelo. Cathorops spixii (Agassiz, 1829), durante o ciclo reprodutivo. Rev. Brasil. Oceanogr. 46(2):171-185.

[19] Sumpter, J.P.; A.P. Scott; S.M. Baynes & P.R. Winttames. 1984. Early stages of the reproductive cycle in virgin female rainbow trout (Salmo gairdneri Richardson). Aquaculture 43:235-242.

[20] Tyler, C.R. & J.P. Sumpter. 1996. Oocyte growth and development in teleosts. Rev. Fish Biol. Fish. 6:287-318.

[21] Vazzoler, A.E.A.M. 1996. Biologia da reprodução de peixes teleósteos. Teoria e Prática. Maringá, EDUEM, 169p.

[22] Wallace, R.A. & K. Selman. 1981. Celular and dynamic aspects of occyte growth in teleosts. Amer. Zool. 21:325-343.

[23] West, G. 1990. Methods of assessing ovarian development in fish: a review. Aust. Jour. Mar. Freshw. Res. 41(2):199-222.

[24] Yamomoto, K. & H. Yoshioka. 1964. Rhythm of development in the oocyte of medaka, Oryzias latipes. Bull. Fac. Fish. Hokkaido Univ. 15(1):50-19.

[25] Zanuy, S. 1987. Reproduction en aquacultura. Madrid, Caicyt