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Acta Botanica Brasilica

Print version ISSN 0102-3306

Acta Bot. Bras. vol.10 no.2 Feira de Santana Dec. 1996

http://dx.doi.org/10.1590/S0102-33061996000200001 

Métodos de coloração de Roeser (1972) - modificado - e Kropp (1972) visando a substituição do azul de astra por azul de alcião 8GS ou 8GX

 

Staining methods of modified Roeser (1972) and Kropp (1972), aiming at substituing the astra blue by alcian blue 8GS or 8GX

 

 

Rebeca LuqueI; Hildeberto C. de SousaII; Jane Elizabeth KrausIII

IDepart. de Biologia. Facultad de Ciências, Universidad de Los Andes, La Hechicera. Mérida 5101, Venezuela
IIDepart. de Ciências Biológicas. Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Campus do Morro do Cruzeiro, CEP 35400-000 Ouro Preto, MG. Brasil
IIIDepart. de Botânica, Instituto de Biociências, Universidade de São Paulo, C.P. 11461, CEP 05422-970 São Paulo, SP. Brasil

 

 


RESUMO

Em folhas de Lychnophora ericoides, Coffea arabica e Nymphaea mexicana foram testados métodos de coloração baseados em Roeser (1972) modificado e Kropp (1972), visando a substituição do corante azul de astra por azul de alcião 8GS ou 8GX. As amostras foram fixadas em FAA, desidratadas em série butílica terciária e incluídas em parafina. Os cortes histológicos transversais foram corados segundo diferentes baterias de coloração, modificadas quanto ao tipo de corante usado, diferenciador e série de desidratação. As lâminas permanentes foram preparadas com bálsamo-do-canadá sintético. Os resultados obtidos evidenciaram que o método de Roeser (1972) modificado é melhor que o de Kropp (1972), nas condições deste experimento. O azul de astra pode ser substituído por azul de alcião 8GX e a desidratação pode ser em série isopropílica ou etílica, sem grande diferença entre elas. São discutidos os resultados provenientes das diferentes colorações.

Palavras-chave: método de coloração, azul de astra. azul de alcião, fucsina básica, safranina, histologia vegetal


ABSTRACT

Staining methods based on modified Roeser (1972) as well as that of Kropp (1972) were done with leaves of Lychnophora ericoides, Coffea arabica and Nymphaea mexicana aiming at substituing the astra blue stain by alcian blue 8GS or 8GX. Samples were fixed in FAA, dehydrated in teit-butyl alcohol series and embedded in paraffin. The histological transversal sections were stained in different staining batteries, modified according to the type of stains and differentiators, and the series of dehydration. Permanent slides were prepared with synthetic Canada balsam. The results obtained indicate that the modified Roeser method (1972) is better than that of Kropp (1972) under present experimental conditions. The astra blue can be substituted by alcian blue 8GX and dehydration can be isopropylic or ethylic, with little difference between them. The results obtained with the different staining techniques used are discussed.

Key words: staining method, astra blue, alcian blue, basic fuchsin, safranin, plant histology


 

 

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Agradecimentos

Ao CNPq, pela bolsa de pesquisa concedida (Proc. nº 301776/83). Aos Professores Doutores Berta Lange de Morretes, Liliana Forneris e Paulo Alberto Otto, pela tradução dos textos em alemão.

 

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Recebido em 14/12/95.
Aceito em 2/6/96.

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