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Mycological Diversity Description II

ABSTRACT

Here, Diaporthe myracrodruonis is introduced as new species from Brazil, isolated as endophyte from Myracrodruon urundeuva. Asterina mandaquiensis is epitypified and ilustrated for the first time. Serpula similis is reported as new to the Neotropics, while Perenniporia centrali-africana is reported for the first time as endophyte and Preussia africana as endophyte from Spondias tuberosa in Caatinga in Brazil.

Keywords:
Asterinales; Boletales; CaM; Diaporthales; ITS rDNA; LSU rDNA; Pleosporales; Polyporales; tef1-α; TUB2

Perenniporia centrali-africana Decock & Mossebo, Sytematics and Geography of Plants 71 (2): 608 (2001)

(Fig. 1)

Figure 1
Perenniporia centrali-africana. A-B. Culture on PDA after 10 days (B. reverse); C. Skeletal hyphae. D-F. Chlamydospores-like structures. G-H. Generative hyphae with clamp connection (arrowed). Scale bars: 10 µm.

Culture characteristics: Colonies on PDA growing up to 50 × 55 mm diam. after 10 days at 25 oC, cottony, without pigment, surface white, reverse plate white. Colonies on MEA 45 × 50 mm diam., cottony, surface white, reverse plate white. Hyphal system dimitic to trimitic, generative hyphae with clamp-connection, thin-walled, hyaline, branched, 2-3 μm wide; skeletal hyphae thick-walled, unbranched, hyaline, slightly dextrinoid in Melzer’s reagent, 2-4 μm wide. Connective hyphae branched and sinuous, thin, without clamp-connection, 2 μm wide. Chlamydospore-like structures terminal or intercalary, smooth, globose to sub-globose, 10 × 5 μm. Reproductive structures basidia and basidiospores absent.

Material examined: BRAZIL, Pernambuco: Petrolândia, isolated as endophyte in leaves of Citrullus lanatus crops (Cucurbitaceae), 25 July 2016, R.M.F. Silva (strain: URM 7859; GenBank MH330690).

Notes - Perenniporia Murrill is a cosmopolitan genus with the ability to colonize various habitats and substrates and has been reported as endophytes in several plants, such as stems of Theobroma gileri (Evans et al. 2003Evans HC, Holmes KA, Thomas SE. 2003. Endophytes and mycoparasites associated with an indigenous forest tree, Theobroma gileri, in Ecuador and a preliminary assessment of their potential as biocontrol agents of cocoa diseases. Mycological Progress 2: 149-160. ), leaves of palm (Pinruan et al. 2010Pinruan U, Rungjindamai N, Choeyklin R, Lumyong S, Hyde KD, Jones EBG. 2010. Occurrence and diversity of basidiomycetous endophytes from the oil palm, Elaeis guineensis in Thailand. Fungal Diversity 41:71-88.) and leaves of bamboo (Zhou et al. 2017Zhou YK, Shen XY, Hou CL. 2017. Diversity and antimicrobial activity of culturable fungi from fishscale bamboo (Phyllostachys heteroclada) in China. World Journal of Microbiology and Biotechnology 33: 104. doi: 10.1007/s11274-017-2267-9
https://doi.org/10.1007/s11274-017-2267-...
). The type material of P. centrali-africana was first reported on dead wood of an angiosperm in Cameroon (Decock & Mossebo, 2001Decock C, Mossebo DC. 2001. Studies in Perenniporia (Basidiomycetes, Aphyllophorales): African taxa II. Perenniporia centrali-africana, a new species from Cameroon. Systematics and Geography of Plants 71: 607-612. ). Since the original description, there has been only one other report of this species in the world, which was found on decaying wood in Brazil (Crous et al. 2017Crous PW, Wingfield MJ, Burgess TI. et al. 2017. Fungal Planet description sheets: 558-624. Persoonia 38: 240-384.). Perenniporia centrali-africana was identified based on phylogenetic analysis using ITS rDNA sequences, and is reported here for the first time as endophyte. In addition, chlamydospore-like structures are also reported for the first time for the species. The sequence obtained showed 99% identity with other sequences deposited as P. centrali-africana (GenBank: KX584433). The phylogenetic analysis resolved our sequence as closely related to other sequences of P. centrali-africana (Fig. 2).

Figure 2
Phylogenetic tree of the Perenniporia constructed using ITS rDNA sequences, showing the position of P. centrali-africana isolated from watermelon leaves. The sequence obtained in this study is in boldface. Support values are from Bayesian inference and maximum likelihood analyses, respectively.

Serpula similis (Berk. & Broome) Ginns, Mycologia 63(2): 231 (1971Ginns J. 1971. The genus Merulius IV. Species proposed by Berkeley and Curtis, and by Berkeley and Broome. Mycologia 63: 219-236. )

= Merulius similis Berk. & Broome, J. Linn. Soc., Bot. 14(no. 73): 58 (1873) [1875]

Description (updated from Ginns 1971Ginns J. 1971. The genus Merulius IV. Species proposed by Berkeley and Curtis, and by Berkeley and Broome. Mycologia 63: 219-236.): basidiomata effuse, effuse-reflexed to pileate, separable from the substrate, semicircular, up to 4 cm in radius, approximately 2 mm thick at the base, soft. Upper surface pale brown with small darker patches where touched; glabrous, smooth when fresh; slightly irregularly folded or wrinkled due to shrinking when dry. Hymenial surface yellow-brown, brittle, somewhat shiny, with a reticulate to merulioid pattern of shallow, reticulate to irregular, shallow pores, 2−3 pores per mm, becoming more shallow towards margin, tubes brown, dense, fragile (01.−0.3 mm deep). Margin buff to pallid or tan, thick, pruinose or matted tomentose, up to 1 mm wide; rhizomorphs lacking; context pallid, spongy, approximately 1 mm thick, homogeneous. Hyphal system mono- to dimitic, context hyphae interwoven, generative hyphae hyaline, thin-walled, with clamp connections (1.5−7.5 µm wide), skeletal hyphae refractive, thick-walled, often with no lumen, very occasionally branched, nonseptate, often abruptly constricted to smaller diameter (0.4−4 µm wide); crystals numerous in context, scattered but not incrusting hyphae. Basidiospores bright yellow, thick-walled, smooth, ellipsoid to broadly-ovate, adaxially flattened [4−5 (−7) × 3− 3.5 (−4.5) µm], IKI−.

Material examined: BRAZIL. Amapá: Porto Grande, Floresta Nacional do Amapá, Sept. 2013, Feb. 2014, Oct. 2014, Feb. 2015, A. Soares (URM 89847, URM 89848, URM 89849, URM 89851, URM 89852). CAMEROON. Campo: Akok Lowland Rain Forest. Dec 1991, M. Nuñez & L. Ryvarden (O F909008). GHANA. Prov. Ashanti Region: Bibiri Forest Reserve. Oct, 2010. M. M. Apetorgbor (O F505801). THAILAND. Cangwat Chiang Mai: Amphoe Muang. Aug 1978. T. Schumacher (O F909006). ZAIRE. Irangi (Kivu): Primary Rain Forest. Apr 1972. J. Rammeloo (O F909005).

GenBank accession numbers: URM 89847: ITS MH458401, LSU MH404846; URM 89851: ITS MH453543, LSU MH453544.

Notes: This is the first record of S. similis from the Neotropics. It was originally described from Sri Lanka and it was previously reported from tropical regions in Africa, Southeast Asia and Australia (Skrede et al. 2011Skrede I, Engh I, Binder M, Carlsen T, Kauserud H, Bendiksby M. 2011. Evolutionary history of Serpulaceae (Basidiomycota): molecular phylogeny, historical biogeography and evidence for a single transition of nutritional mode. BMC Evolutionary Biology 11: 230-243.). In the phylogenetic tree, the specimens of S. similis from Brazil clustered with specimens from Australia and Thailand with strong support (100 %/1.00). The specimens from Brazil (Fig. 3) are characterized by distinctly pileate basidiomata, white to pale cream (when fresh) and yellow to dark orange (when dried). The hyphal system is monomitic, with generative hyphae with larges clamps (3−5 (m). These morphological features are slightly different from the original description, which reports an effused basidioma and a dimitic hyphal system. Although many studies describe S. similis as having an effused basidioma (Ginns 1971Ginns J. 1971. The genus Merulius IV. Species proposed by Berkeley and Curtis, and by Berkeley and Broome. Mycologia 63: 219-236.; Carlier et al. 2004Carlier F−X, Bitew A, Castillo G, Decock C. 2004. Some Coniophoraceae (Basidiomycetes, Boletales) from the Ethiopian highlands: Coniophora bimacrospora, sp. nov. and a note on the phylogenetic relationships of Serpula similis and Gyrodontium. Cryptogamie Mycologie 25: 261-275.), analysis from the material deposited in the Herbarium of the University of Oslo (O) revealed that some specimens from Africa are also pileate (O F505801; O F909008). Initially, the Brazilian specimens were thought to be a new species due to these morphological differences, but the molecular data showed that they represent only a variation of the species.

Figure 3
Serpula similis(URM 89847): A. Fresh basidiomata. B. Hymenial Surface. C. Generative hyphae with clamp connections. D. Yellow basidisopores. Scale bars: a, b=10 cm. c, d= 5 μm. Figure: A.M.S. Soares and R. Alvarenga. The macro- (morphology, color, consistency, size) analyses were performed on both fresh and dehydrated basidiomata. Free-hand sections from hymenium and context were visualized under optical microscopy using 3 % KOH + 1 % phloxine, and Melzer’s reagent (Ryvarden 1991Ryvarden L. 1991. Genera of polypores, nomenclature and taxonomy. Synopsis Fungorum 5: 1-363.) Color designation followed Watling (1969Watling R. 1969. Colour identification chart. Edinburgh, Her Majesty`s Stationery Office.).

Serpula costaricensis M. Mata & Ryvarden is another similar species. As in S. similis, the basidioma is distinctly pileate; however, it is larger and more robust and has ellipsoid, bigger basidiospores [6−7 (8) × 4−5 µm] (Mata & Ryvarden 2007Mata M, Ryvarden L. 2007. New and interesting species from Costa Rica. Synopsis Fungorum 23: 51-55.). Serpula fuscescens, the only species reported to Brazil so far, has encrusted cystidia and subglobose to ovoid basidiospores [(4.5−) 5−6 × 4−5 µm] (Nakasone 2008Nakasone KK. 2008. Type studies of corticioid Hymenomycetes described by Bresadola. Cryptogamie Mycologie 29: 231-257.).

As showed in the phylogenetic tree, S. similis is closely related to the S. tignicola (Harmsen) M.P. Christ. with high statistical support (89/1.00) (Fig. 4). Serpula tignicola is a European species and differs from S. similis by the resupinate basidioma with folded to raduloid hymenium and rhizomorphs (Harmsen 1952Harmsen L. 1952. Merulius tignicola sp. nov. og dens forekomst i Danmark. Friesia 4: 243-256.; Christiansen 1960Christiansen MP. 1960. Danish resupinate fungi. Part II. Homobasidiomycetes. Dansk botanisk Arkiv. 19: 57-388.). Besides, these two species show considerable genetic difference, as can be seen from the branches in the phylogram (Fig. 4). This particular attribute was also noticed by Carlier et al. (2004Carlier F−X, Bitew A, Castillo G, Decock C. 2004. Some Coniophoraceae (Basidiomycetes, Boletales) from the Ethiopian highlands: Coniophora bimacrospora, sp. nov. and a note on the phylogenetic relationships of Serpula similis and Gyrodontium. Cryptogamie Mycologie 25: 261-275.) in their analyses using only nLSU and by Skrede et al. (2011Skrede I, Engh I, Binder M, Carlsen T, Kauserud H, Bendiksby M. 2011. Evolutionary history of Serpulaceae (Basidiomycota): molecular phylogeny, historical biogeography and evidence for a single transition of nutritional mode. BMC Evolutionary Biology 11: 230-243.), using five molecular markers. The latter study suggested that the long branch indicates an increased rate of molecular evolution in this lineage.

Figure 4
Phylogenetic tree inferred from ITS and LSU rDNA gene sequences using Bayesian and maximum likelihood analyses with the GTR + G evolution model retrieved by PartitionFinder v1.1.0 (Lanfear et al. 2012Lanfear R, Calcott B, Ho SYW, Guindon S. 2012. PartitionFinder: combined selection of partitioning schemes. Molecular Biology and Evolution 29: 1695-1701.). Bayesian and likelihood trees showed the same topology displayed here. Branch lengths reflect estimated number of 0.2 changes per site. Bayesian analysis was performed with MrBayes 3.2.1 software (Ronquist et al. 2012Ronquist F, Teslenko M, Mark P, et al. 2012. MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61: 1-4.) for 10 million generations with four Markov chains. Likelihood analysis was performed with the same evolutionary model under 1000 rearrangements using RAxML (Stamatakis 2014Stamatakis A. 2014. Raxml version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30: 1312-1313.). Bootstrap support (BS) values after 1000 rearrangements and posterior probabilities are indicated on tree nodes. Branches with PP > 0.95 and BS from 90 % are considered as strongly supported.

Phylogenetically, S. similis and S. tignicola are distantly related to the clade where S. lacrymans, the type species of Serpula, is placed, indicating that they belong to a different genus. However, additional morphological and molecular analyses of Serpula s.l. through the inclusion of additional specimens and markers, are necessary for a better circumscription of the genus.

Asterina mandaquiensis Henn., Hedwigia 48: 12. 1908.

MycoBank MB 223742, Figs. 56

Figure 5
Asterina mandaquiensis (VIC42824, epitype). A. Colonies on leaf surface. B. Colony with open thyriothecia and surface mycelium. C. Thyriothecia opened by a central star-shaped fissure. D. Thyriothecium fringed at margins. E. Branched mycelium with appressoria. F. Alternate to unilateral appressoria. G. Ovoid ascus with immature hyaline ascospores. H. Brown and smooth mature bicelular ascospores. Scale bars: A= 4 mm; B= 200 μm; C-E= 50 μm; D-F-G= 20 μm; H= 10 μm.

Figure 6
Phylogenetic tree obtained from Bayesian Inference analysis using the sequences of the LSU rDNA obtained from data set of 17 taxa including representatives of Asterinales stricto sensu, lato sensu and the epitype of Asterina mandaquiensis (highlighted in bold). Capnodium coaratum MFLUCC 10006 and Capnodium coffeicola MFLUCC 150206 were used as outgroup.

Description: Colonies epiphyllous, circular, single to confluent, black, 0.5-4 mm diam. Hyphae straight to slightly flexuous, branching unilaterally or alternately, ferruginous to brown, septate, hyphal cells cylindrical, 4.5-5 μm diam. (2-3.5 μm), smooth. Appressoria numerous, entire, sessile, lateral, alternate to unilateral, never opposed, ovate, unicellular, facing forward, 6-10 × 5-7 μm, brown, penetration peg central on the appressorial cell. Ascomata superficial, thyriothecia, scutiform, on top of mycelial mat, circular, single to confluent, fringed at margins, randomly distributed in the colony, 142-218 μm diam. (200-250 μm diam.), opening by a central star-shaped fissure, dark brown to blackish; wall of textura radiate, cells isodiametric to cylindrical. Pseudoparaphyses cylindrical, filiform, septate, unbranched, hyaline to yellowish, up to 1.5 μm wide. Asci bitunicate in structure, fissitunicate, disposed as an upright palisade layer, ovoid, 8-spored, hyaline, 50-65 × 25-35 μm (35-50 × 20-30 μm). Ascospores cylindrical to ellipsoid, ends rounded, straight to arched, 1-septate, constricted at the median septum, hyaline, becoming brown at maturity, smooth, 22.5-27.5 × 7.5-10.5 μm (20-25 × 8-10 μm). Asexual morph not observed. Measurements of the structures in the original description are in parentheses.

Material examined: BRAZIL. Minas Gerais: Viçosa, Mata da Biologia (20°45'32.375"S 42°51'44.154"W), on living leaves of Eugenia uniflora L. (Myrtaceae), May 2012, A.L. Firmino (VIC42824, epitype designated here).

GenBank accession number: LSU MH780924; ITS MH780973

Notes - The specimen described above was collected in the state of Minas Gerais on living leaves of Eugenia uniflora. The original material was described by Hennings (1908Hennings P. 1908. Fungi S. Paulensis IV a cl. Puttemans collecti. Hedwigia 48: 1-20.) based on material from the Mandaqui, a neighbourhood in São Paulo city, Brazil, on leaves of the same host. Mandaqui is part of Serra da Cantareira, and the type material was collected by Martio in 1903, however, with the extensive expansion of the city of São Paulo, the native vegetation was totally destroyed, making it impossible to recollect this material at the same place. This species is illustrated here for the first time.

Phylogenetic analyses reveal that the A. mandaquiensis is well segregated from the other members of the family Asterinaceae (Fig. 6).

Preussia africana Arenal, Plantas & Peláez, Fungal Diversity 20: 6. 2005.

Fig. 7

Figure 7
Preussia africana (URM 7936). A. Ascoma, asci and ascospores. B-C. Developing asci with young ascospores. D. Asci and ascospores. Scale bars: A: 20 µm; B-D: 10 µm.

Description: Ascomata scattered, partially immersed in culture media. Pseudothecia scattered and isolated, mostly below the mycelium and partially immersed in culture, globose to pyriform, ostiolate, brown to dark brown, (52-)91-99(-143) × 52-78(-104) µm in diameter. Peridium dark brown, glabrous, pseudoparenchymatous in surface view, membranaceous, coriaceous. Pseudoparaphyses filiform, septate, interspersed with asci. Asci bitunicate, without opening, apex rounded, 39-47(-65) × (13-)15.5-18 µm, short and robust stipe up to 9 × 5 µm, cylindrical-clavate, eight-spored. Ascospores uniseriate to biseriate, cylindrical, dark brown; four-celled, cells separable at the central septa with shallow constrictions, rounded apex, 26-28.5 × 5-5.5 µm; gelatinous sheath hyaline and narrow.

Cultures (in the dark, 25 oC, 2 wk): Colonies on PDA attaining 13 mm diam. and on MEA 29 mm diam. Texture floccose, initially white to light cream in color, occasionally submerged, reverse from dark brown to black (according to the color charts of Rayner 1970Rayner RW. 1970. A mycological colour chart. London, Commonwealth Mycological Institute.).

Material examined: Preussia africana. BRAZIL. Pernambuco: Petrolina, isolated as an endophyte from leaves of Spondias tuberosa Arruda (Anacardiaceae), May 2017, V.M. Svedese and L.C. Oliani (strain: URM 7936 = isolate 63V).

GenBank accession number: ITS MH220329.1.

Notes - The genus Preussia was proposed by Fuckel (1867Fuckel L. 1867. Fungi Rhenani Cent. 18 (1), no 1702-1764. Hedwigia 6: 174-175.) to accommodate species with cleistothecioid ascomata and associated with plant debris, wood or soil (Kruys & Wedin 2009Kruys A, Wedin M. 2009. Phylogenetic relationships and an assessment of traditionally used taxonomic characters in the Sporormiaceae (Pleosporales, Dothideomycetes, Ascomycota), utilising multi-gene phylogenies. Systematics and Biodiversity 7: 465-478. ). The relationship between Preussia and Sporormiella has been questioned by several authors. They are considered synonyms due to their similar morphological features and results of phylogenetic analyses (Kruys & Wedin 2009Kruys A, Wedin M. 2009. Phylogenetic relationships and an assessment of traditionally used taxonomic characters in the Sporormiaceae (Pleosporales, Dothideomycetes, Ascomycota), utilising multi-gene phylogenies. Systematics and Biodiversity 7: 465-478. ; Mapperson et al. 2014Mapperson RR, Kotiw M, Davis RA, Dearnaley JDW. 2014. The diversity and antimicrobial activity of Preussia sp. endophytes isolated from australian dry rainforests. Current Microbiology 68: 30-37.; Kruys 2015Kruys A. 2015. New species of Preussia with 8-celled ascospores (Sporormiaceae, Pleosporales, Ascomycota). Phytotaxa 234: 143-150.; Crous et al. 2018Crous PW, Wingfield MJ, Burgess TI, et al. 2018. Fungal Planet description sheets: 716-784. Persoonia 40: 240-393.). Only Preussia, along with five other accepted genera, is included in the family Sporormiaceae in the Outline of Ascomycota: 2017 by Wijayawardene et al. (2018Wijayawardene NN, Hyde KD, Lumbsch HT, et al. 2018. Outline of Ascomycota: 2017. Fungal Diversity 88: 167-263.). The species treated herein, P. africana, was proposed by Arenal et al. (2005Arenal F, Platas G, Peláez F. 2005. Two new Preussia species defined based on morphological and molecular evidence. Fungal Diversity 20: 1-15.) based on morphological and molecular analyses of a series of isolates of the genus Preussia from different substrates, such as goat dung, zebra dung and Viburnum tinus L. leaves. Furthermore, P. africana has been isolated as an endophytic fungus from different plant species (Brum et al. 2012Brum MCP, Araújo WL, Maki CS, Azevedo JL. 2012. Endophytic fungi from Vitis labrusca L. (“Niagara Rosada”) and its potential for the biological control of Fusarium oxysporum. Genetics and Molecular Research 11: 4187-4197.; Gonzalez-Menendez et al. 2017Gonzalez-Menendez V, Martin J, Siles JA, et al. 2017. Biodiversity and chemotaxonomy of Preussia isolates from the Iberian Peninsula. Mycological Progress 16: 713-728.). Phylogenetic analysis (Fig. 8) using the ITS rDNA sequence of our isolate placed it in the same clade with sequences from the type material and other sequences deposited as P. africana. The morphological analysis of our isolate, obtained as endophyte, differs in some regards with the description of the type material, mainly concerning pseudothecia diameter (180-290 μm), and size of asci (94-110 × 15-17 μm) and ascospores (32.5-44 × 4-7 μm) (Arenal et al. 2005Arenal F, Platas G, Peláez F. 2005. Two new Preussia species defined based on morphological and molecular evidence. Fungal Diversity 20: 1-15.). This is the first report of P. africana isolated as endophytic fungus from leaves of S. tuberosa (Anacardiaceae) in Caatinga forest in Brazil.

Figure 8
Bayesian inference tree obtained using ITS rDNA sequences of 10 representative species of Preussia performed in MrBayes on XSEDE in the CIPRES science gateway (Miller et al. 2010Miller MA, Pfeiffer W, Schwartz T. 2010. Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: Proceedings of the gateway computing environments workshop (GCE) 14 Nov 2010. New Orleans, Institute of Electrical and Electronics Engineers. p. 1-8.) using the model SYM+G as estimated by the MrModelTest v. 2.3 (Nylander 2004Nylander JAA. 2004. MrModelTest v2. Program distributed by the author. Uppsala, Evolutionary Biology Centre, Uppsala University.). The new sequence obtained in this study is in bold. Stemphylium botryosum (CBS 116596) was used as an outgroup.

Diaporthe myracrodruonis A.P.S.L. Pádua, T.G.L. Oliveira, Souza-Motta, Fan & J.D.P. Bezerra, sp. nov.

MycoBank MB 828865, Fig. 9

Figure 9
Diaporthe myracrodruonis (URM 7972 - ex-type culture). A-B. Colony on PDA after 15 days. C-F. Conidiogenous cells. G. Alpha conidia. H. Alpha and beta conidia. Scale bars: 10 µm.

Etymology: The name refers to the host genus from which it was isolated, Myracrodruon.

Description: Conidiomata pycnidial in culture, globose to subglobose with thin wall, solitary or aggregated, dark brown to black, (424-)636-954(-975) × (286-)318-795(-901) μm, with whitish to light cream conidial drops exuding from the ostioles. Conidiogenous cells phialidic, hyaline, smooth wall, occasionally branched, straight to sinuous, thick at base tapering towards the apex, moderately curved apex, (5-)6-9(-11) × 1.5-2(-2.5) μm. Alpha conidia aseptate, hyaline, smooth, fusoid or elongated ellipsoid attenuating toward both ends, 2.5-3.5(-4) × 1-1.5 μm. Beta conidia sickle-shaped, aseptate, hyaline, smooth, truncated base, curved apex slightly tapered, 18-20(-24) × 0.7-1 μm. Sexual morph not observed.

Cultures (in the darkness, 25 oC): Colonies on PDA attaining 9 cm diam., with formation of pycnidia after 15 days. Aerial mycelium of floccose texture, initially white becoming yellowish to brown, with reverse from yellowish to light brown.

Type: BRAZIL. Pernambuco: Serra Talhada (7°57'21.38"S 38°17'43.92"W), isolated as endophyte from leaves of Myracrodruon urundeuva Allemão (Anacardiaceae), July 2016, A.P.S.L. Pádua (Holotype URM 92587; Culture ex-type URM 7972).

GenBank accession number: URM 7972 (ex-type): ITS MK205289, CaM MK205290, tef1-α MK213408, TUB2 MK205291.

Notes - The genus Diaporthe has been widely revised, with new species frequently being described (Gomes et al. 2013Gomes RR, Glienke C, Videira SIR, Lombard L, Groenewald JZ, Crous PW. 2013. Diaporthe: a genus of endophytic, saprobic and plant pathogenic fungi. Persoonia 31: 1-41.; Gao et al. 2015Gao YH, Su YY, Sun W, Cai L. 2015. Diaporthe species occurring on Lithocarpus glabra in China, with descriptions of five new species. Fungal Biology 115: 295-309.; Yang et al. 2018Yang Q, Fan XL, Guarnaccia V, Tian CM. 2018. High diversity of Diaporthe species associated with dieback diseases in China, with twelve new species described. MycoKeys 39: 97-149.). The strain URM 7972 was isolated from leaves of Myracrodruon urundeuva in Brazil (Pádua et al. 2018Pádua APSL, Freire KTLS, Oliveira TGL, et al. 2019. Fungal endophyte diversity in the leaves of the medicinal plant Myracrodruon urundeuva in a Brazilian dry tropical forest and their capacity to produce L-asparaginase. Acta Botanica Brasilica 33: 39-49.) and identified as Diaporthe myracrodruonis sp. nov. The ex-type strain URM 7972 clustered as an independent lineage in the current phylogenetic tree (Fig. 10). BLASTn searches using ITS sequence of D. myracrodruonis demonstrated 96 % similarity to D. infecunda (LGMF940, GenBank KC343133.1). Based on CaM sequence, D. myracrodruonis is 98 % identical to D. infecunda (CBS 133812, GenBank KC343368.1). For tef1-α, the new species has low identity (95-96 %) to sequences deposited as D. beilharziae, D. middletonii and D. infecunda, and 99% similarity to sequences deposited as D. infecunda (GenBank MG265941 and MG265940). The TUB2 sequence of D. myracrodruonis has 96-98 % identity to sequences deposited as D. acaciarum, D. infecunda, and D. middletonii. The most closely related species, Diaporthe infecunda, was isolated from medicinal plants in Brazil, and its description was based on phylogenetic analyses using sequences from five genes (Gomes et al. 2013). Morphologically, D. myracrodruonis differs from D. beilharzie in the size of pycnidia (250-300 μm) and alpha (5.5−10 × 2-3 μm) and beta (15-25 × 1.0-1.5 μm) conidia (Tan et al. 2013Tan YP, Edwards J, Grice KRE, Shivas RG. 2013. Molecular phylogenetic analysis reveals six new species of Diaporthe from Australia. Fungal Diversity 61: 251-260.); from D. acaciarum in the size of pycnidia (up to 300 μm diam.) and alpha (6-7.5 × 2-3 μm) and beta (20-40 × 1.5-2 μm) conidia (Crous et al. 2014Crous PW, Wingfield MJ, Schumacher RK, et al. 2014. Fungal Planet description sheets: 281-319. Persoonia 33: 212-289.); and from D. middletonii in the size of pycnidia (up to 300 μm diam.) and alpha (5-8 × 2-3 μm) and beta (20-35 × 1.0-1.5 μm) conidia (Thompson et al. 2015Thompson SM, Tan YP, Shivas RG, et al. 2015. Green and brown bridges between weeds and crops reveal novel Diaporthe species in Australia. Persoonia 35: 39-49.). Diaporthe myracrodruonis is the first species of Diaporthe described as an endophytic fungus from leaves of Myracrodruon urundeuva in the Brazilian tropical dry forest (Caatinga).

Figure 10
Bayesian inference tree obtained using ITS rDNA, CaM, tef1-α and TUB2 sequences of species of Diaporthe performed using MrBayes on XSEDE in the CIPRES science gateway with the substitution models GTR+I+G (ITS) and HKY+G (CaM, tef1-α and TUB2). The Maximum likelihood analysis using the model GTR+I+G was conducted in RAxML in CIPRES using the combined dataset. BPP and ML-BS above 0.95 and 70 %, respectively, are shown near nodes. The new species is in bold face. Diaporthella corylina (CBS 121124) was used as an outgroup.

Acknowledgements

For research fellowships and/or financial support ALF, AMS, APSLP, CMSM, GAS, HLP, JDPB, LR, MACM, OLP, RJVO, RMFS, TBG, TGLO thank CAPES and CNPq, AMS, APSLP, CMSM, HLP, JDPB, LR, TBG, TGLO thank FACEPE, and ALF, OLP thank FAPEMIG. AMS, HLP, LR, TBG thank ICMBio and IBAMA for support during field trips, and Msc. RLM Alvarenga for the figures 3 and 4. JDPB and TGLO thank Uitamara dos Santos for the help collecting plant material.

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Publication Dates

  • Publication in this collection
    Jan-Mar 2019

History

  • Received
    17 May 2018
  • Accepted
    04 Feb 2019
Sociedade Botânica do Brasil SCLN 307 - Bloco B - Sala 218 - Ed. Constrol Center Asa Norte CEP: 70746-520 Brasília/DF. - Alta Floresta - MT - Brazil
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