SciELO - Scientific Electronic Library Online

 Useful plants referenced by the naturalist Richard Spruce in the 19th century in the state of Pará, Brazil author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Acta Botanica Brasilica

Print version ISSN 0102-3306On-line version ISSN 1677-941X

Acta Bot. Bras., ahead of print  Epub Mar 25, 2019 


Spores of Plagiochila (Dumort.) Dumort.: the taxonomic relevance of morphology and ultrastructure

Juliana da Costa Silva-e-Costa1  *

Andrea Pereira Luizi-Ponzo2

1 Programa de Pós-Graduação em Ecologia, Departamento de Botânica, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, 36036-900, Juiz de Fora, MG, Brazil

2 Departamento de Botânica, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, 36036-900, Juiz de Fora, MG , Brazil


Plagiochilaceae is a family of leafy liverworts that are distributed worldwide. It is of great importance due to its taxonomic and ecological implications among bryophytes. Most species of the family belong to the genus Plagiochila, but there is no consensus regarding its infrageneric circumscription. There have been few palynological studies involving Plagiochilaceae and Plagiochila. Here, we describe the spore morphology of seventeen species of Plagiochila and discuss the taxonomic value of palynological characters for these taxa. The spores were processed by standard palynological techniques and analyzed using light and electron microscopy. The spores were found to be apolar, spheroidal, released monads that vary in size from 13µm to 58µm (small to large size). The sporoderm comprises an intine (stratified), a nexine, and a sexine. The spore surface is ornamented with granules that vary in shape and morphology, thus allowing the studied species to be grouped into four spore types: regular and delicate granulate, irregular and coarse granulate, long granules with flattened apices, and long and straight granules. Hierarchical cluster analysis revealed five different groups of species, evidencing the importance of spore information for taxonomic and phylogenetic studies.

Keywords: bryophytes; liverworts; morphology; palynology; SEM; taxonomy; TEM; ultrastructure


Plagiochilaceae (Lophocoleineae) is a family of leafy liverworts that includes robust, ascending or pendent plants (Heinrichs 2002; Gradstein & Costa 2003). Their leaves are alternate or opposite, succubous and with a ciliated or entire reflexed dorsal margin, while underleaves are generally absent (Gradstein et al. 2001; Gradstein & Costa 2003). Plagiochilaceae is an important group of bryophytes in the tropics due to its high species richness (Gradstein & Reiner-Drehwald 1995), but it is also diverse in subtropical and temperate regions (Jamy et al. 2016). The family contains ten genera that are distributed worldwide (Crandall-Stotler et al. 2009; Söderström et al. 2013; 2016), with Plagiochila being the richest by far with ca 96 % of the species of the family (Gradstein et al. 2001; Heinrichs 2002; Gradstein & Costa 2003; Jamy et al. 2016; Söderström et al. 2016).

Plagiochila is a taxonomically complex group, with more than 2300 published names (Inoue 1989), and maybe as many as approximately 3000 names (ELPT database). So & Grolle (2000) reported ca 450 species distributed worldwide, whereas Gradstein (2015a; b; 2016) reviewed and contributed to synonymization and lectotypification of several names, and so more recent estimates place the number of currently accepted species at 700 (Söderström et al. 2016).

Numerous attempts at infrageneric classification of Plagiochila have been made, with early attempts relying on some important characters of the gametophyte, such as leaf shape, leaf cell pattern and branching type (Lindenberg 1839; Spruce 1885; Schiffner 1900; Stephani 1902; Dugas 1929; Carl 1931). Later, studies of Schuster (1959; 1960), Inoue & Schuster (1971), and more recently by So (2001), and Hässel (2004; 2006), used gametophyte morphology for the classification of species of Plagiochila. Molecular phylogenetic studies of Plagiochila and Plagiochilaceae (Heinrichs 2002; Groth et al. 2003; Patzak et al. 2016; Jamy et al. 2016; Söderström et al. 2016) have reviewed the morphological classification of these taxa with the aim of improving the current taxonomic arrangement.

In spite of the various studies that have been conducted with Plagiochila, information about its spores is scarce (Erdtman 1965; Vojtkó 1993) or limited to brief comments in taxonomic descriptions (Grolle & Heinrichs 1999; Heinrichs & Gradstein 1999; Müller et al. 1999; Heinrichs et al. 2000; 2001; Heinrichs 2002; Hässel 2004; among others). Erdtman (1965) and Vojtkó (1993) presented a general description of spore surface and an evaluation of size, but with a small sample size. Descriptions of the spores of the genus in taxonomic studies are limited to superficial and sometimes inaccurate comments on ornamentation and size measurements.

Several characteristics of plants were crucial for their evolutionary transition to a terrestrial life, but the development of a durable and protective spore wall was essential (Brown & Lemmon 1988; Renzaglia et al. 2000; Wellman 2004; Wallace et al. 2011; Arteaga-Vazquez 2016). The spore - a single cell produced by meiosis - is the first stage of the gametophyte in the life cycle of liverworts (Brown & Lemmon 1988). Liverworts possess two layers of sporoderm, the special cell wall of the spore: an inner layer called the intine, which is composed of polysaccharides and is related to spore germination; and an external stratum called the exine, which composed of sporopollenin, a highly resistant polymer that provides resistance and protection (Olesen & Mogensen 1978; Neidhart 1979; Mogensen 1983; Blackmore & Barnes 1987; Brown & Lemmon 1988; Ito et al. 2007; Renzaglia et al. 2000; Wallace et al. 2011).

Many studies on spores have addressed different aspects of various groups (Heckman 1970; Blackmore & Barnes 1987; Brown & Lemmon 1980; 1984a; b; 1988; 1991; Estébanez et al. 1997; Luizi-Ponzo & Barth 1998; 1999; Luizi-Ponzo & Melhem 2006; Caldeira et al. 2006; 2009; 2013; Yano & Luizi-Ponzo 2006; 2011; Rocha et al. 2008; Rodrigues & Luizi-Ponzo 2015; Savaroğlu 2015; Savaroğlu et al. 2017; Silva-e-Costa et al. 2017). However, little is known about morphology of the spores of the genus Plagiochila. In fact, it is worthy to note that sporophytes are infrequent among species of Plagiochila (Heinrichs 2002).

The present study aimed to perform a palynological evaluation of species of Plagiochila in order to: (1) analyze intra- and interspecific variation in spore size, (2) describe spore ornamentation and (3) sporoderm structure, and (4) determine whether infrageneric circumscriptions are supported by spore morphology.

Materials and methods

Sample selection and studied material

The research was developed using herborized botanical material loaned or donated by the following herbaria: Botanical Garden of Rio de Janeiro Herbarium (RB), Brazilian National Museum Herbarium (R), Herbarium Anchieta (PACA), Santa Cecília University Herbarium (HUSC), University of Kentucky Herbarium (KY), and Professor Leopoldo Krieger Herbarium (CESJ). Acronyms follow Thiers (2018).

As previously mentioned, the rarity of the occurrence of sexual reproduction in Plagiochila (Dumort.) Dumort. is remarkable, which makes finding sporophytes on these plants difficult (Schuster 1980; Heinrichs 2002; Gradstein & Costa 2003). Thus, 1000 specimens from the aforementioned herbaria of various species of Plagiochila were examined in search of specimens with sporophytes. All species for which a sporophyte was found, and which and enough material available for study, were analyzed, for a total of thirty-four specimens of seventeen species. The analyzed species, preceded by the name of their respective section, are: sect. Arrectae Carl - Plagiochila bifaria (Sw.) Lindenb; sect. Fuscolutea Carl - P. fuscolutea Taylor; sect. Glaucescentes Carl - P. buchtiniana Steph.; sect. Hylacoetes Carl - P. macrostachya Lindenb.; sect. Plagiochila - P. asplenioides (L.) Dumort. and P. porelloides (Tor ex. Ness) Lindenb.; sect. Rutilantes Carl - P. gymnocalycina (Lehm. & Lindenb.) Lindenb., P. heteromalla Lehm. & Lindenb., P. rutilans Lindenb., and P. trichostoma Gottsche.; and sect. Vagae Lindenb. - P. corrugata (Nees) Nees & Mont, P. crispabilis Lindenb., P. disticha (Lehm. & Lindenb.) Lindenb., P. laetevirens Lindenb., P. patula (Sw.) Lindenb., P. raddiana Lindenb., and P. simplex (Sw.) Lindenb. Species circumscription follows Gradstein (2015b) and the names are in accordance to the Tropicos Database (

Light microscopy

For observation under light microscopy (LM), spores were prepared according to the method of Wodehouse (1935), for observation of cellular content, and by the acetolysis method proposed by Erdtman (1960). Both techniques were performed following the modifications recommended by Luizi-Ponzo & Melhem (2006). Spores were described using the terminology proposed by Punt et al. (2007) and the definitions of size classes of Erdtman (1952).

Scanning electron microscopy

For observation under scanning electron microscopy (SEM), capsules were fixed in 2.5 % glutaraldehyde for 24 hours and then washed in 0.05 M phosphate buffer solution. Post-fixation was performed with 2 % osmium tetroxide (OsO4) in buffer solution for a period of two hours. The capsules were then dehydrated in an increasing ethanol series and dried in a critical point dryer (Silveira 2007). The capsules were opened under stereoscopic microscopy and the spores dispersed on stubs with double-sided carbon tape and covered with a 20 nm layer of gold. Non-fixed spores were also observed. The SEM analyses were undertaken at the Laboratório de Microscopia Eletrônica of the Universidade Federal de Juiz de Fora, the Centro de Microscopia of the Universidade Federal de Minas Gerais, and the Centro de Microscopia e Microanálise of the Universidade Federal de Viçosa.

Transmission electron microscopy

For observation under transmission electron microscopy (TEM), mature capsules were separated, fixed in 2.5 % glutaraldehyde for 24 hours, washed in 0.05 M phosphate buffer solution, and post fixed in 2 % osmium tetroxide in buffer solution. After dehydration in an increasing ethanol series, the material was embedded in Spurr resin and heated at 70 ºC for 48 hours. The material was cut in ultrathin sections (65-70 nm) and stained with uranyl acetate and lead citrate (Reynolds 1963). The TEM analyses were undertaken at the Microscopy Center of Federal University de Minas Gerais.

Statistical analyses

Spore size was assessed under light microscopy using acetolyzed material. When possible, based on the availability of palynological material, more than one specimen was examined for all species studied and with a reference specimen (RS) and comparison specimens (CS) being designated. For estimating largest diameter, 50 RS spores randomly chosen from three microscope slides were measured. When available, 30 CS spores randomly chosen from three microscope slides were analyzed. Descriptive statistics were calculated from the resulting data, including arithmetic mean (X), size range (Xmin-Xmax), standard deviation (S), standard error (Sx), coefficient of variation (CV% - obtained by the formula (S/X)*100) (Sokal & Rohlf 1995), 95 % confidence level (95 % CL), and 95 % confidence interval (95 %CI - X ± 95 %CL) (Sokal & Rohlf 1995) using Microsoft Excel (2016). Ten non-acetolyzed RS spores randomly chosen from three microscope slides were measured for sporoderm thickness and only the arithmetic mean calculated.

The measurements were not normally distributed (Shapiro-Wilk normality test, p < 0.05) and so the Kruskall-Wallis test, followed by the Dunnett’s test (which are more appropriate tests for non-parametric data), were performed to test intra- and interspecific differences. Median values and data distribution were graphically evaluated. Statistical analysis and graphing were performed using R software v. 3.5.1 (R Development Core Team 2018) and JMP® 12 (SAS Institute, Cary, North Carolina, USA).

Cluster analysis

The degree of association among the studied species was evaluated by cluster analysis, using the unweighted pair-group average (UPGMA) algorithm and Jaccard similarity index, and calculating the cophenetic correlation coefficient, using the software Past 3.21 (Hammer et al. 2001).

Palynological, gametophytic and ecological data (Tab. 1) were organized in a qualitative binary matrix (Tab. 2). The palynological data included the following: spore size (< 26 µm or 26 µm), spore ornamentation (granules or long granules), and sporoderm thickness (two classes of sporoderm thickness were established using the formula h=A/k; where h is the class amplitude, A is the spore size amplitude, and k is the number of classes (Correa 2007). The gametophytic data included the following: branching type (Frullania-type or Plagiochila-type), androecia shape (single or fan-shaped), androecia position (terminal or intercalary), perianth base (naked or covered by bracts), and asexual reproduction (absent or present). The single ecological variable included was related to substratum: the species are reported as exclusive when occurring on only one type of substratum, and generalist when occurring on two or more types of substrata. The species Plagiochila bifaria was not included in this analysis due to a lack of information.

Table 1  Palynological, gametophytic and ecological aspects of the studied species of Plagiochila (Dumort.) Dumort. 

Species Spore Size Spore Ornamentation Sporoderm thichness Branching type Substratum occupation Androecia shape Androecia position Perianth base Asexual reproduction Section
P. asplenioides Small RD 1.5327 Frullania Exclusive Single Intercalary Bracts Absent Plagiochila
P. buchtiniana Medium LS 1.2636 Frullania Exclusive Single Intercalary Bracts Absent Glaucescentes
P. corrugata Medium LS 1.3923 Frullania Exclusive Single Intercalary Bracts Absent Vagae
P. crispabilis Medium LF 1.6497 Frullania Generalist Fan-Shaped Terminal Bracts Propagules Vagae
P. disticha Medium RD 1.2519 Frullania Generalist Single Terminal, Intercalary Bracts Propagules Vagae
P. fuscolutea Large LS 1.5444 Plagiochila Generalist Single Intercalary Bracts Absent Fuscolutea
P. gymnocalycina Small IC 2.0241 Plagiochila Generalist Single Intercalary Naked Caducous leaves Rutilantes
P. heteromalla Medium IC 1.4742 Plagiochila Exclusive Single Terminal Naked Caducous leaves and fragments Rutilantes
P. laetevirens Medium IC 1.3221 Frullania Generalist Sngle Terminal, Intercalary Bracts Propagules Vagae
P. macrostachya Medium LS 1.8369 Plagiochila Generalist Fan-Shaped Terminal Bracts Caducous leaves Hylacoetes
P. patula Medium RD 1.1700 Frullania Generalist Single Intercalary Bracts Propagules Vagae
P. porelloides Small IC 1.1457 Plagiochila Exclusive Single Terminal, Intercalary Bracts Absent Plagiochila
P. raddiana Medium IC 0.9594 Frullania Generalist Single Terminal Bracts Propagules Vagae
P. rutilans Medium LS 1.8837 Plagiochila Generalist Single Intercalary Bracts Caducous leaves Rutilantes
P. simplex Small LF 1.5561 Plagiochila Generalist Single Intercalary Naked Absent Vagae
P. trichostoma Medium LS 1.2168 Plagiochila Generalist Single Intercalary Bracts Caducous leaves and fragments Rutilantes

Table 2  Binary matrix showing the arrangement of palynological, gametophytic and ecological aspects of Plagiochila (Dumort.) Dumort. Spore size: <26 µm = 1, ≥ 26 µm = 0; spore ornamentation: types RD and IR = 0, types LF and LS = 1; sporoderm thickness: ≥ 1.4900 µm = 1; < 1.4900 µm = 0; branching: Plagiochila-type = 1; Frullania-type = 0; Substratum occupation: exclusive = 1, generalist = 0; androecia shape: fan-shaped = 1, single = 0; androecia position: intercalary = 1, terminal = 0; perianth base: bracts present = 1, naked = 0; asexual reproduction: present = 1, absent = 0. 

Species Size Ornamentation Sporoderm Thickness Branching Type Substrum Occupation Androecia Shape Androecia Position Perianth Base Asexual Reproduction
P. asplenioides 1 0 1 0 1 0 1 1 0
P. buchtiniana 0 1 0 0 1 0 1 1 0
P. corrugata 0 1 0 0 1 0 1 1 0
P. crispabilis 0 1 1 0 0 1 0 1 1
P. disticha 1 0 0 0 0 0 1 1 1
P. fuscolutea 0 1 1 1 0 0 1 1 0
P. gymnocalycina 1 0 1 1 0 0 1 0 1
P. heteromalla 0 0 0 1 1 0 0 0 1
P. laetevirens 0 0 0 0 0 0 1 1 1
P. macrostachya 0 1 1 1 0 1 0 1 1
P. patula 0 0 0 0 0 0 1 1 1
P. porelloides 1 0 0 1 1 0 1 1 0
P. raddiana 1 0 0 0 0 0 0 1 1
P. rutilans 0 1 1 1 0 0 1 1 1
P. simplex 1 1 1 1 0 0 1 0 0
P. trichostoma 1 1 0 1 0 0 1 1 1


The spores of the genus Plagiochila are monads that are isomorphic, apolar to weakly heteropolar, small to large in size (13.00 - 57.80 µm; Tab. 3, Figs. 1, 2), inaperturate and with a subcircular amb. Spore surface ornamentation is formed by granules, but the nature of these processes varies among the seventeen species studied (Figs. 1, 2), with four types being identified under LM and SEM: (1) regular and delicate granulate (RD), (2) irregular and coarse granulate (IC), (3) long granules with flattened apices (LF), and (4) long and straight granules (LS).

Table 3  Morphometric data of acetolyzed spores of Plagiochila. Reference specimen indicated by an asterisk (n=50); comparison specimens, n=30. 

Material (Xmin-Xmax) X ± Sx S 95 % IC CV (%)
Plagiochila asplenioides (L.) Dumort.
Hanner, 5020 (MN) * 13.00 - 26.00 18.79 ± 0.36 2.60 18.06 - 19.52 13.84
Plagiochila bifaria (Sw.) Lindenb.
M.C. Vaughan Bandeira (166934/RB)* 20.80 - 31.20 24.50 ± 0.32 2.28 23.85 - 25.15 9.30
Plagiochila buchtiniana Steph.
J. Heinrichs et al., 4128 (RB) * 41.60 - 52.00 46.54 ± 0.38 2.74 45.77 - 47.31 5.88
Plagiochila corrugata (Nees) Nees & Mont.
L.A. Paiva, 320 (CESJ) * 24.70 - 36.40 29.22 ± 0.38 2.70 28.46 - 29.98 9.24
A. Sehnem, 2407 (PACA) 22.10 - 31.20 26.21 ± 0.42 2.34 25.37 - 27.05 8.92
A. Sehnem, 1103 (PACA) 19.50 - 39.00 27.84 ± 0.71 3.92 26.38 - 29.30 14.08
A. Sehnem, 4742 (PACA) 26.00 - 37.70 30.57 ± 0.58 3.20 29.38 - 31.76 10.46
Plagiochila crispabilis Lindenb.
D.P. Costa et al., 5100 (RB) * 23.40 - 33.80 27.89 ± 0.30 2.18 27.27 - 28.5 7.81
D.M. Vital, 4946 (RB) 23.40 - 36.40 28.21 ± 0.39 2.19 27.40 - 29.02 7.76
P. Occhioni, s/n (166931/ RB) 18.20 - 31.20 25.22 ± 0.59 3.27 24.00 - 26.44 12.96
Plagiochila disticha (Lehm. & Lindenb.) Lindenb.
D.P. Costa et al., 2895 (RB) * 15.60 - 23.40 20.18 ± 0.23 1.68 19.70 - 20.66 8.32
D.P. Costa et al., 2769 (RB) 20.80 - 31.20 24.32 ± 0.39 2.11 23.52 - 25.12 8.67
D.P. Costa et al., 2685 (RB) 20.80 - 28.60 25.93 ± 0.41 2.22 25.09 - 26.77 8.56
D.P. Costa et al., 2615 (RB) 18.20 - 28.60 22.99 ± 0.44 2.41 22.09 - 23.89 10.48
D.P. Costa et al., 2623 (RB) 31.20 - 20.80 25.87 ± 0.48 2.67 24.88 - 26.86 10.32
Plagiochila fuscolutea Taylor
H. Anton & J. Heinrichs (373220/RB) 46.80 - 72.80 57.05 ± 0.89 6.35 55.25 - 58.85 11.13
J. Henrichs, 3915 (RB) 31.20 - 52.00 37.65 ± 0.92 4.99 35.76 - 39.54 13.25
Plagiochila gymnocalycina (Lehm. & Lindenb.) Lindenb.
N.D. Santos et al.,840 (RB) * 18.20 - 31.20 23.46 ± 0.41 2.91 22.63 - 24.29 12.40
D.P.Costa, 3890 & S.R. Gradstein (RB) 19.50 - 27.30 23.27 ± 0.36 1.99 22.52 - 24.02 8.55
D.P.Costa, 3876 & S.R. Gradstein (RB) 15.60 - 23.40 19.34 ± 0.45 2.45 18.41 - 20.27 12.66
Plagiochila heteromalla Lehm. & Lindenb.
N.D. Santos et al., 475 (RB) * 26.00 - 39.00 30.94 ± 0.38 2.71 30.17 - 31.71 8.75
Plagiochila laetevirens Lindenb.
M. Bandeira (223901/RB) * 20.80 - 33.80 26.17 ± 0.37 2.66 25.42 - 26.92 10.16
Plagiochila macrostachya Lindenb.
E. T. Amorim, P2 (CESJ) * 21.45 - 36.40 26.87 ± 0.38 2.3 26.10 - 27.64 10.16
Plagiochila patula (Sw.) Lindenb.
D.P. Costa et al., 5054 (RB) * 18.20 - 41.60 28.16 ± 0.61 4.30 26.94 - 29.38 15.26
D.P. Costa et al., 2885 (RB) 15.60 - 23.40 19.24 ± 0.48 2.63 18.26 - 20.22 13.66
Plagiochila porelloides (Torr. ex. Ness) Lindenb.
D.N. McLetchie, s/n (UKY) * 14.30 - 23.40 19.43 ± 0.36 2.56 18.71 - 20.15 13.17
Plagiochila raddiana Lindenb.
E.C. Rente, 426 (MN) * 10.40 - 18.20 13.80 ± 0.24 1.70 13.32 - 14.28 12.31
D.P. Costa et al., 2659 (RB) 16.25 - 23.40 20.29 ± 0.40 2.17 19.47 - 21.11 10.69
D.P. Costa et al., 5062 (RB) 20.80 - 31.20 26.06 ± 0.52 2.86 25.00 - 27.12 10.97
D.P. Costa et al., 2596 (RB) 20.80 - 28.60 23.44 ± 0.37 2.06 22.68 - 24.20 8.78
Plagiochila rutilans Lindenb.
M.C. Vaughan Bandeira (166936/RB) * 27.00 - 41.60 34.16 ± 0.48 3.45 33.18 - 35.14 10.09
D. Sucre 2453. & P.I.S. Braga (RB) 28.60 - 39.00 36.31 ± 0.52 3.23 35.11 - 37.51 8.89
Plagiochila simplex (Sw.) Lindenb.
P. Dusén, 450 (MN) * 16.90 - 26.00 21.36 ± 0.27 1.95 20.81 - 21.91 9.12
D.P. Costa et al., 4863 (RB) 15.60 - 28.60 22.44 ± 0.45 2.50 21.51 - 23.37 11.14
Plagiochila trichostoma Gottsche
J. Heinrichs et al., 4323 (RB)* 20.80 - 31.20 25.15 ± 0.38 2.69 24.39 - 25.91 10.69

Figure 1  Photomicrographs and electromicrographs of spores of species of Plagiochila (Dumort.) Dumort. Ornamentation type I - A. Plagiochila asplenioides, LM; B. P. patula, LM; C. P. patula, SEM; Ornamentation type II - D. P. gymnocalycina, LM; E. P. laetevirens, LM; F - G. P. porelloides, SEM - arrow with asterisks = proximal face; H. P. raddiana, SEM; I. P. disticha, TEM - arrow = lamelar slips. RD = regular and delicate granule; IC = irregular and coarse granule. In I: In = inner intine, Io = outer intine, E = exine. 

Figure 2  Photomicrographs and electromicrographs of spores of species of Plagiochila (Dumort.) Dumort. Ornamentation type III - A. Plagiochila crispabilis, LM; B. P. simplex, LM; C. P. crispabilis, SEM - arrow with asterisks = proximal area; D-E. P. simplex, SEM -asterisks = proximal face; F. P. simplex, TEM; G. P. trichostoma, LM; H. P. corrugata, SEM; I. P. trichostoma, SEM. LF = long granule with flattened apex; LS = long and straight granule. In F: In = inner intine, Io = outer intine, E = exine, arrow = lamellar slips. 

Ornamentation pattern I - RD consists of granules homogeneously distributed on the spore surface and having a regular shape (Fig. 1A - C). This pattern is possessed by Plagiochila asplenioides (Fig. 1A), P. disticha and P. patula (Fig. 1B, C). Spore size for these three species varies from small to medium (Tab. 3), and the sporoderm is thin (Tab. 4). Under LM this ornamentation appears as a blur in optical cut due to its small size and fine magnification (Fig. 1A, B), but under SEM the granules are easily identified (Fig. 1C).

Table 4  Morphometric data for sporoderm thickness of spores of Plagiochila (n=10). 

Species Size Ornamentation Sporoderm Thickness Branching Type Substrum Occupation Androecia Shape Androecia Position Perianth Base Asexual Reproduction
P. asplenioides 1 0 1 0 1 0 1 1 0
P. buchtiniana 0 1 0 0 1 0 1 1 0
P. corrugata 0 1 0 0 1 0 1 1 0
P. crispabilis 0 1 1 0 0 1 0 1 1
P. disticha 1 0 0 0 0 0 1 1 1
P. fuscolutea 0 1 1 1 0 0 1 1 0
P. gymnocalycina 1 0 1 1 0 0 1 0 1
P. heteromalla 0 0 0 1 1 0 0 0 1
P. laetevirens 0 0 0 0 0 0 1 1 1
P. macrostachya 0 1 1 1 0 1 0 1 1
P. patula 0 0 0 0 0 0 1 1 1
P. porelloides 1 0 0 1 1 0 1 1 0
P. raddiana 1 0 0 0 0 0 0 1 1
P. rutilans 0 1 1 1 0 0 1 1 1
P. simplex 1 1 1 1 0 0 1 0 0
P. trichostoma 1 1 0 1 0 0 1 1 1

Ornamentation pattern II - IC - comprises irregular shaped granules disorderly distributed on the spore surface (Fig. 1D-H). This pattern is possessed by P. gymnocalycina (Fig. 1D), Plagiochila heteromala, P. laetevirens (Fig. 1E), P. porelloides (Fig. 1F, G) and P. raddiana (Fig. 1H). Spore size varies from small to medium (Tab. 3), and the sporoderm is of variable thickness (Tab. 4). Plagiochila gymnocalycina has a more elaborate version of this pattern with overlapped and united granules (Fig. 1D), producing a gemmae-like appearance.

Ornamentation pattern III - LF - includes species whose spore surface is ornate with elongate and irregularly distributed granules that have flattened apexes (Fig. 2A- F). This pattern is possessed by Plagiochila crispabilis (Fig. 2A, C) and P. simplex (Fig. 2B, D-F). This type of granule is barely observable under LM (Fig. 2A), but can be clearly observed under SEM, including the flattened apex region (Fig. 2C-F). There is a region with smaller granules restricted to a particular area (Fig. 2D) that suggests it may have been the site of contact during the tetrad stage.

Ornamentation pattern IV - LS - this ornamentation consists of an ornate spore surface with elongate granules that have a smooth and straight shape, resembling bacula (Fig. 2G-I). The species that possess this pattern are: Plagiochila bifaria, P. buchtiniana, P. corrugata (Fig. 2H), P. fuscolutea, P. macrostachya, P. rutilans, and P. trichostoma (Fig. 2G, I). A region with smaller granules, suggestive of the proximal pole, was observed in some species. Among this group, P. corrugate is notable for having endosporic and intracapsular germination (Fig. 2H).

Sporoderm thickness varied from 0.9 µm to 1.8 µm among the studied species (Tab. 4). Observations under TEM revealed the sporoderm to comprise one or two electron-translucent layer(s) corresponding to the intine (Figs. 1I, 2F - inner intine and outer intine when two layers are present), and two electron-dense layers compounded by lamellae deposition (Figs. 1I, 2F), corresponding to the exine divided into nexine and sexine. The sexine lamellae have a perpendicular to inclined arrangement (Figs. 1I, 2F). A stratified intine was observed for the spores of P. disticha (Fig. 1I), including a granular inner layer, with a mix of electron-translucent and electron-dense components, that is in contact with cell contents, and an outer layer, with a great amount of electron-translucent elements, that is in contact with the nexine.

Descriptive statistical analyses showed that the species analyzed here differ significantly in spore size (Fig. 3). Furthermore, there was significant intraspecific variation in spore size (Fig. 4) for those species for which CS was available (see Tab. 3), with the exception of P. simplex (Fig. 4I), which did not exhibit significant intraspecific variation in spore size.

Figure 3  Boxplots representing the spore size distribution within Plagiochila (Dumort.) Dumort. Error bars above and below the box indicate the 90th and 10th percentiles, respectively, while white circles represent the outliers. 

Figure 4  Boxplots representing the spore size distribution within those species for which RS and CS were observed. Error bars above and below the box indicate the 90th and 10th percentiles, respectively, while white circles represent the outliers. Different letters (a, b, c, d, e) represent statistical differences among treatments (Krukal-Wallis test, Dunn’s post hoc test, p < 0.05). 

The variability of spore size was confirmed by coefficients of variation (Tab. 3). The lowest values, around 8 %, were for P. buchtiniana and RS and CS1 in P. cripabilis. Values around 10 % were observed in a great number of species, while values greater than 12 % were detected in at least one specimen of P. asplenioides, P. crispabilis, P. fuscolutea, P gymnocalycina, P. raddiana P. patula, and P. porelloides. For almost all the studied species for which a CS could be analyzed, the mean value for the CS did not fit the CI established for the reference specimen (Tab. 3).

Cluster analysis revealed five groups with similarity above 0.5 (Fig. 5 - G1 to G5) and a cophenetic correlation coefficient of 0.8138. The group G1 assembles P. disticha, P. laetevirens, P. patula, and P. raddiana; group G2 group unites P. asplenioides, P. buchtiniana, P. corrugata, and P. porelloides; group G3 is the largest, being represented by P. fuscolutea, P. gymnocalycina, P. rutilans, P. trichostoma, and P. simplex; group G4 is formed by P. crispabilis and P. macrostachya; and group G5 is represented by the single species P. heteromalla. Referring to the binary matrix (Tab. 4), it is possible to unravel the characteristics shared among species of the same group. The species of G1 share ornamentation type (rounded granules), sporoderm thickness, Frullania-type branching, generalist substratum occupation, single androecia, perianth covered by bracts, and some type of asexual reproduction. The species of G2 also share a single androecia and a perianth covered by bracts, but do not possess a asexual reproduction structure, and are exclusive in substratum occupation. In addition, these species also share androecia positioned terminally, although and branching type is variable. The species of G3 have generalist substratum occupation, Plagiochila-type branching and single androecia; while the species of G4 have generalist substratum occupation, fan-shaped androecia, and perianth covered by bracts. The species of this last group also share some spore characteristics such as ornamentation and sporoderm thickness. The species P. heteromalla is the only species of group G5.

Figure 5  Representation of the hierarchical cluster analyses showing the five groups of species of Plagiochila (Dumort.) Dumort. UPGMA algorithm, Jaccard similarity index, Cophenetic correlation = 0.8130. 


Plagiochila is an important genus, being distributed worldwide and one of the most speciose genera of liverworts. Palynological information can lead to a better understanding of the taxonomy and ecology of the species of Plagiochila. The present study revealed the spores of this genus to be variable, especially with regard to spore size and sporoderm ornamentation, which were able to separate the studied species into four spore types.

Spore size in Plagiochila

Average spore size in Plagiochila varied from 13.80 µm to 57.05 µm, being classified as small to large (Erdtman 1952). Spore size is a variable characteristic among liverworts. For example, spores of Chonecolea doelligeri (Chonecoleaceae) measured 16.00 µm in polar view (Yano & Luizi-Ponzo 2006), while species belonging to Frullaniaceae (Zhao et al. 2011), Dumortieraceae (Yano & Luizi-Ponzo 2011), Porellaceae (Zhao et al. 2011), and Ricciaceae (Steinkamp & Doyle 1979; Zhao et al. 2011), varied widely from 31 µm (medium-sized) to 127 µm (giant).

Related to the present study, Erdtman (1965) reported that the spores of P. asplenioides have an average size of 15 µm, while Vojtkó (1993) reported the spores of P. porelloides to be 17.80 µm. Some taxonomic studies have included notes about spores of species of Plagiochila, especially regarding size and surface ornamentation. Heinrichs & Gradstein (2000) described the spores of P. disticha as ranging in size from 16 µm to 28 µm (- 47 µm), and those of P. raddiana ranging 18 µm - 25 µm (- 45 µm); Heinrichs et al. (2000) reported a range of 33 µm - 54 µm for spores of P. buchtiniana ; Heinrichs et al. (2001) reported a range of 23 µm - 28 µm for spores of P. rutilans; and Heinrichs et al. (2004a) reported 18 µm - 52 µm for P. corrugata. The present study found slightly narrower ranges of variation for the spores of these species.

Lophocoleineae Schljakov, which includes Plagiochilaceae, possesses exosporic spore germination (Crandall-Stotler et al. 2009), but some studies (Heinrichs et al. 2000; 2004a; Heinrichs 2002) have reported spores of Plagiochila released with 1-8 cells. Endosporic germination was also observed in the present study with the release of spores with 1-5 cells (and even intracapsular germination) in P. corrugata.

The coefficient of variation for spore size for the species studied here ranged from 8 % to 12 %. A coefficient of variation of around 10 % has been commonly found by palynological treatments of bryophytes, such as Luizi-Ponzo & Barth (1998; 1999), Luizi-Ponzo & Melhem (2006), Rocha et al. (2008), Caldeira et al. (2009; 2013), Yano & Luizi-Ponzo (2011), and Rodrigues & Luizi-Ponzo (2015). Heinrichs (2002) stated that the average size of spores of Plagiochila vary considerably, while Heinrichs & Gradstein (1999) reported ca. 50 % variation in spore size for Plagiochila longiramea.

Sporoderm structure and surface ornamentation

Two layers of sporoderm, the intine and the exine, were observed in the species of the present study, which is typical of for bryophyte spores (Clarke 1979; Neidhart 1979; Mogensen 1983). Erdtman (1965) reported that spores of P. asplenioides have a very thin exine. The sporoderm of the species studied here is indeed quite thin, being no more than 2 µm thick and even thinner than 1 µm for some species.

Analyses made under TEM revealed that the exine is divided into the nexine, an inner layer, and the sexine, an outer layer. Similar exine configurations have been observed for spores of both mosses and liverworts (Heckman 1970; Steinkamp & Doyle 1979; Brown & Lemmon 1988; Estébanez et al. 1997; Yano & Luizi-Ponzo 2006; 2011; Rocha et al. 2008; Caldeira et al. 2013; Brown et al. 2015; Silva-e-Costa 2015; Rodrigues & Luizi-Ponzo 2015).

Lamellar deposition of exine elements in liverworts was first reported by Heckman (1970) and Brown & Lemmon (1988). The present study found perpendicular sexine elements in two species - P. disticha and P. simplex. Heckman (1970) reported similar sexine patterns in two other liverworts - Lophocolea heterophylla and Chiloscyphus polyanthos. This author described these elements that shape spore ornamentation as “lamellar slips”. Both species studied by Heckman (1970) belong to the family Lophocoleaceae, which is, as is Plagiochilaceae, a member of Lophocoleineae.

Two light electron-dense layers were identified in P. disticha, which correspond to intine. The intine is the last layer to be formed and is directly related to spore germination (Neidhart 1979; Mogensen 1983; Brown & Lemmon 1988). Mogensen (1983) pointed out that the intine can have little stratification, as observed in the present study; Studying the spore wall structure in Jungermanniales, Heckman (1970) presented a similar configuration of a stratified intine in L. heterophylla; even though it was not mentioned in the text, the stratification is easy recognized in the image provided by the paper.

McClymont & Larson (1964) described a multistratified intine for Archidium alternifoliuim; Nilsson (1990) observed it in pollen grains of Apocynaceae; Estébanez et al. (1997) observed a one to three-layered intine in Grimmia; Luizi-Ponzo & Melhem (2006) described a stratified intine for mature spores of Helicophyllum torquatum; and, finally, Medina & Estébanez (2014) reported intine stratification for Orthotrichum ibericum and Orthotrichum striatum, and argued that intine stratification may be due to the environmental and developmental condition of spores.

Different dispositions of the exine element may be related to early contact at the proximal pole during spore wall formation. Brown & Lemmon (1991) observed that during wall formation of liverwort spores, especially in Jungermanniidae, the exine is generally thinner and less ornamented on the proximal face than in other areas of the spore wall, and might represent a region for germination.

Some authors have described some Plagiochila spores as ornate with bacula (Heinrichs & Gradstein 2000; Heinrichs et al. 2001; 2004a; 2005a; b; Heinrichs 2002), verrucate, or even vermiculate (Inoue 1982; Muller et al. 1999; Heinrichs 2002; Groth et al. 2003; Heinrichs et al. 2004b). In a treatment of Plagiochilaceae of North America, Schuster (1980) described the ornamentation of the spores as “finely granulose”. In a compilation of literature data, Heinrichs (2002) described two basic patterns of spore ornamentation for Plagiochila: (1) verrucate-vermiculate structures, and (2) baculate structures (varying from “bacula sensu strictu” to pila). Observing the images provided by these authors, in can be concluded that these ornamentation patterns are, actually, granules with different and variable shapes and morphologies. Punt et al. (2007) defined bacula as “a cylindrical, free standing exine element more than 1 µm in length and less than this in diameter”. In a similar way, a verruca is an element more than 1 µm wide; and vermiculate is used to describe rugulate pollen and spores. Thus, none of these terms fit the spore ornamentation observed in Plagiochila. The present study encountered elements that were less than 1µm long and wide, and when they were larger than this the elements were clearly groups of united processes. Heinrichs (2002) stated that the spores of Plagiochila are, necessarily, trilete with a weakly developed laesure under SEM observation. Due to their thin and delicate spore wall, spores of species of Plagiochila are easily damaged during processing for SEM, thus giving the appearance of an irregular trilete mark. However, when spores are observed under different kinds of preparation, this affirmation is clearly denied. Observations of spores under LM (Figs. 1A, B, D, E; 2A, B, G) prior to acetolysis, affirm that they are not trilete, but indeed tremendously fragile and easily folded. This fragility is related to the thin sporoderm and, especially, the delicacy of the nexine, which becomes folded during processing for SEM (Figs. 1F, H; 2C, E, H). It is important to note, however, that in spite of this fragility, the sporoderm tolerates acetolysis due its sporopollenin content (Mogensen 1983; Brown & Lemmon 1988).

Interpretation of hierarchical clustering and the taxonomic implications

The hierarchical clustering analyses revealed five groups with a cophenetic correlation coefficient higher than 0.8, which represents low distortion and relatively reliable data (Rohlf & Fisher 1968). These five groups, G1 to G5, did not correspond to the current infrageneric classification of Plagiochila. The common characteristics shared by species of the same group, for groups G1 to G4, are mostly morphological characteristics of the gametophyte. Furthermore, species with the same ornamentation type described in this study were also separated by cluster analysis. To G1 and G4, indeed, spore ornamentation was a shared variable for species of group G1 and for species of group G4, while surface ornamentation varied within groups G2 and G3. Although these groups did not correspond directly to either infrageneric circumscriptions or ornamentation types, the palynological information added to gametophyte morphology clearly formed new groups, suggesting that spore data may contribute to understanding the phylogenetic relationships within Plagiochila. Spore morphology is genetically based (Clarke 1979), and thus can be evolutionary information and useful in phylogenetic analyses.

Palynology can make an important contribution to taxonomic studies, especially when dealing with taxa that present taxonomic difficulties. The species of Plagiochila of the present study are quite homogeneous with regard to gametophyte characteristics, but they differ in spore structure, size, sporoderm strata, and surface ornamentation. As demonstrated here, different ornamentation types were observed and can useful in species descriptions.


We thank the curators of the herbaria CESJ, HUSC, KY, PACA, R, and RB, and especially Nicholas McLecthie and Robert Paratley. Thanks also go to: Universidade Federal de Juiz de Fora/ Pós-graduação em Ecologia for logistical support and Pedro Loureiro for technical assistance. This research was financed by the Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) (Grant APQ CRA 01598-14) and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) - Finance Code 001.


Arteaga-Vazquez AM. 2016. Land plant evolution: listen to your elders. Current Biology 26(1). doi: 10.1016/j.cub.2015.12.001 [ Links ]

Blackmore S, Barnes SH. 1987. Embryophye spore walls: Origin, development, and homologies. Cladistics 3: 185-195. [ Links ]

Brown RC, Lemmon BE. 1980. Ultrastructure of sporogenesis in a moss: Ditrichum pallidum. III. Spore wall formation. American Journal of Botany 67: 918-934. [ Links ]

Brown RC, Lemmon BE. 1984a. Ultrastructure of sporogenesis in moss Amblystegium riparium II. Spore wall development. Journal of Hattori Laboratory 57: 139-152. [ Links ]

Brown RC, Lemmon BE. 1984b. Spore wall development in Andreaea (Musci: Bryopsida). American Journal of Botany 71: 412-420. [ Links ]

Brown RC, Lemmon BE. 1988. Sporogenesis in bryophytes. Advances in Bryology 3: 159-223. [ Links ]

Brown RC, Lemmon BE. 1991. Sporogenesis in simple land plants. In: Blackmore S, Barnes SH. (eds.) Pollen and spores. patterns of diversification. Oxford, Clarendon Press. p 9-24. [ Links ]

Brown RC, Lemmon BE, Shimamura M, Villarreal JC, Renzaglia KS. 2015. Spores of relictual bryophytes: Diverse adaptations to life on land. Review of Palaeobotany and Palynology 216: 1-17. [ Links ]

Caldeira IC, Gonçalves-Esteves V, Luizi-Ponzo AP. 2006. Morfologia dos esporos das espécies de Leucobryaceae Schimp. (Bryophyta) do Parque Estadual de Ilha Grande, Município de Angra dos Reis, Estado do Rio de Janeiro. Revista Brasileira de Botânica 29: 301-307. [ Links ]

Caldeira IC, Gonçalves-Esteves V, Luizi-Ponzo AP. 2009 Morfologia dos esporos de Sematophyllaceae Broth. ocorrentes em três fragmentos de Mata Atlântica, no Rio de Janeiro, Brasil. Revista Brasileira de Botânica 32: 299-306. [ Links ]

Caldeira IC, Gonçalves-Esteves V, Luizi-Ponzo AP. 2013. Palynology of selected species of Fissidens (Hedw.). Plant Systematics and Evolution 299: 187-195. [ Links ]

Carl H. 1931. Die Arttypen und die systematische Gliederung der Gattung Plagiochila Dum. Annales Bryologici 2: 1-170. [ Links ]

Clarke GCS. 1979. Spore morphology and bryophyte systematics. In: Clarke GCS, Duckett JG. (eds.) Bryophyte systematics. Systematic Association Special Vol. 14. London/ New York, Academic Press. p. 231-250. [ Links ]

Correa SMBB. 2007. Probabilidade e estatística. 2nd. edn. Belo Horizonte, PUC Minas Virtual. [ Links ]

Crandall-Stotler B, Stotler RE, Long DG. 2009. Morphology and classification of the Marchantiophyta. In: Shaw JA, Goffinet B. (eds.) Bryophyte biology. Cambridge, Cambridge University Press. p. 1-54. [ Links ]

Dugas M. 1929, Contribution à l’étude du genre “Plagiochila” Dum. Annales des Sciences Naturelles; Botanique, Série 10 11: 1-199. [ Links ]

Erdtman G. 1952. Pollen morphology and plant taxonomy. Angiosperms. An Introduction to Palynology I. Stockholm, Almqvist and Wiksell. [ Links ]

Erdtman G. 1960. The acetolysis method. A revised description. Svensk Botanisk Tidskrift 39: 561-564. [ Links ]

Erdtman G. 1965. Pollen and spore morphology/ plant taxonomy. Gymnospermae, Bryophyta (Text). An introduction to palynology III. Stockholm, Almqvist and Wiksell . [ Links ]

Estébanez B, Alfayate C, Ron E. 1997. Observations on spore ultrastructure in six species of Grimmia (Bryopsida). Grana 36: 347-357. [ Links ]

Gradstein SR. 2015a. New synonyms and new lectotypifications in neotropical Plagiochila (Marchantiophyta). Cryptogamie, Bryologie, 36: 369-379. [ Links ]

Gradstein SR. 2015b. Annotated key to the species of Plagiochila (Marchantiophyta) from Brazil. Pesquisas, Botânica 67: 23-36. [ Links ]

Gradstein SR. 2016. The genus Plagiochila (Marchantiophyta) in Colombia. Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales 40: 104-136. [ Links ]

Gradstein SR, Churchill SP, Salazar-Allen N. 2001. Guide to the bryophytes of Tropical America. New York, Memoirs of the New York Botanical Garden. [ Links ]

Gradstein SR, Costa DP. 2003. The Hepaticae and Anthocerotae of Brazil. New York, Memoirs of the New York Botanical Garden . [ Links ]

Gradstein SR, Reiner-Drehwald E. 1995. Szweykowskia, a new genus of Plagiochilaceae (Hepaticae) from tropical America. Fragmenta Floristica et Geobotanica 40: 31 -38. [ Links ]

Grolle R, Heinrichs J. 1999. Redescription and synonymy of Plagiochila aerea Täylor 1846 (Hepaticae), first describe as Lycopodium pinnatum by Lamarck 1792. Nova Hedwigia 68: 551-525. [ Links ]

Groth H, Lindner M, Wilson R, et al. 2003. Biogeography of Plagiochila (Hepaticae): natural species groups span several floristic kingdoms. Journal of Biogeography 30: 965-978. [ Links ]

Hammer Ø, Harper DAT, Ryan P. 2001. PAST: Paleontological statistics software package for education and data analysis. Palaeontologia Electronica 4: 1-9. [ Links ]

Hässel GG. 2004. Andinopatagonian Species of Plagiochila (Plagiochilaceae, Marchantiophyta). I. Sectio Robustae Carl Emend. Hässel and II. Sectio Equitantes Carl Emend. Hässel. Journal of the Hattori Botanical Laboratory 96: 245-260. [ Links ]

Hässel GG. 2006. Andinopatagonian species of Plagiochila (Plagiochilaceae, Marchantiophyta). I sectio Hirtae and II sectio Angulatae. Journal of the Hattori Botanical Laboratory 100: 101-118. [ Links ]

Heckman CA. 1970. Spore wall structure in the Jungermanniales. Grana 10: 109-119. [ Links ]

Heinrichs J. 2002. A taxonomic revision of Plagiochila sect. Hylacoetes, sect. Adiantoideae and sect. Fuscoluteae in the Neotropics with a preliminary subdivision of Neotropical Plagiochilaceae into nine linages. Berlin, Bryophytorum Bibliotheca. [ Links ]

Heinrichs J, Anton H, Gradstein SR, Mues R. 2000. Systematics of Plagiochila sect. Glaucescentes Carl (Hepaticae) from tropical America: a morphological and chemotaxonomical approach. Plant Systematics and Evolution 220: 115-138. [ Links ]

Heinrichs J, Gradstein SR. 1999. On Plagiochila longiramea Steph., a poorly known species of Bolivia. Candollea 54: 73-81. [ Links ]

Heinrichs J, Gradstein SR. 2000. A revision of Plagiochila sect. Crispatae and sect. Hypnoides (Hepaticae) in the Neotropics. I. Plagiochila disticha, P. montagnei and P. raddiana. Nova Hedwigia 70: 161-184. [ Links ]

Heinrichs J, Groth R, Gradstein SR, Rycroft DS, Cole WJ, Anton H. 2001. Plagiochila rutilans (Hepaticae): A poorly known species from Tropical America. The Bryologist 104: 350-361. [ Links ]

Heinrichs J, Groth H, Lindner M, Renker C. 2005a. Distribution and synonymy of Plagiochila punctata (Taylor) Taylor, with hypotheses on the evolutionary history of Plagiochila sect. Arrectae (Plagiochilaceae, Hepaticae). Plant Systematics and Evolution 250: 105-117. [ Links ]

Heinrichs J, Groth H, Lindner M, Renker C, Pócs T, Pröschold T. 2004a. Intercontinental distribution of Plagiochila corrugata (Plagiochilaceae, Hepaticae) inferred from nrDNA ITS sequences and morphology. Botanical Journal of the Linnean Society 146: 469-481. [ Links ]

Heinrichs J, Lindner M, Gradstein SR, et al. 2005b. Origin and subdivision of Plagiochila (Jungermanniidae: Plagiochilaceae) in tropical Africa based on evidence from nuclear and chloroplast DNA sequences and morphology. Taxon 54: 317-333. [ Links ]

Heinrichs J, Lindner M, Groth H. 2004b. Sectional classification of the North American Plagiochila (Hepaticae, Plagiochilaceae). The Bryologist 107: 489 496. [ Links ]

Inoue H. 1982. Scanning electron microscopy in hepatic taxonomy. Nova Hedwigia 71: 75-80. [ Links ]

Inoue H. 1989. Notes on the Plagiochilaceae, XVI. Studies on some Plagiochila species in the Neotropics. Bulletin of the National Science Museum 15: 35-47. [ Links ]

Inoue H, Schuster RM. 1971. A monograph of the New Zealand and Tasmanian Plagiochilaceae. Journal of the Hattori Botanical Laboratory 34: 1-225. [ Links ]

Ito T, Nagata N, Yoshiba Y, Ohme-Takagi M, Ma H, Shinozakif K. 2007. Arabidopsis MALE STERILITY1 Encodes a PHD-Type Transcription Factor and Regulates Pollen and Tapetum Development. The Plant Cell 19: 3549-3562. [ Links ]

Jamy M, Renner MAM, Patzak SDF, Heslewood MM, Schäfer-Verwimp A, Heinrichs J. 2016. Reinstatement of Plagiochila sect. Abietinae (Plagiochilaceae, Jungermanniopsida). Cryptogamie, Bryologie 37: 351-360. [ Links ]

Lindenberg JBW. 1839. Species hepathicarum. Fasc. 1. Bonn, Henry & Cohen. [ Links ]

Luizi-Ponzo AP, Barth OM. 1998. Spore morphollogy of some Bruchiaceae species (Bryophyta) from Brazil. Grana 37: 222-227. [ Links ]

Luizi-Ponzo AP, Barth OM. 1999. Spore morphology of some Dicranaceae species (Bryophyta) from Brazil. Grana 38: 42-49. [ Links ]

Luizi-Ponzo AP, Melhem TS. 2006. Spore morphology and ultrastructure of the tropical moss Helicophyllum torquatum (Hook.) Brid. (Helicophyllaceae) in relation to systematics and evolution. Cryptogamie, Bryologie 27: 413-420. [ Links ]

McClymont JW, Larson DA. 1964. An electron-microscopic study of spore wall structure in the musci. American Journal of Botany 51: 195-200. [ Links ]

Medina NG, Estébanez B. 2014. Does spore ultrastructure mirror different dispersal strategies in mosses? A study of seven Iberian Orthotrichum species. PLOS ONE 9(11): e112867. doi: 10.1371/journal.pone.0112867 [ Links ]

Mogensen, GS. 1983. The spore. In: Schuster RM. (eds.) New manual of bryology. Nichinan, The Hattori Botanical Laboratory. p. 324-342. [ Links ]

Müller J, Heinrichs J, Gradstein SR. 1999. A revision of Plagiochila sect. Plagiochila in the Neotropics. The Bryologist 102: 729-746. [ Links ]

Neidhart HB. 1979. Comparative studies of sporogenesis in bryophytes. In: Clarke CGS, Duckett JG. (eds.) Bryophyte Systematics. London, Academic Press. p 251-280. [ Links ]

Nilsson S. 1990. Taxonomic and evolutionary significance of pollen morphology in the Apocynaceae. Plant Systematics and Evolution 5: 91-102. [ Links ]

Olesen P, Mogensen GS. 1978. Ultrastructure, histochemistry and notes on germination stages of spores in selected mosses. The Bryologist 81: 493-516. [ Links ]

Patzak SDF, Renner MAM, Schäfer-Verwimp A, et al. 2016. A phylogeny of Lophocoleaceae-Plagiochilaceae-Brevianthaceae and a revised classification of Plagiochilaceae. Organisms Diversity & Evolution 16: 481-495. [ Links ]

Punt W, Hoen P, Blackmore S, Nilsson S, Thomas A. 2007. Glossary of pollen and spore terminology. Review of Palaeobotany and Palynology 143: 1-81. [ Links ]

R Development Core Team. 2018. R: A language and environment for statistical computing. Vienna, R Foundation for Statistical Computing. [ Links ]

Renzaglia KS, Duff RJ, Nickrent DL, Garbary DJ. 2000. Vegetative and reproductive innovations of early land plants: implications for a unified phylogeny. Philosophical Transactions of the Royal Society B: Biological Sciences 355: 769-793. [ Links ]

Reynolds ED. 1963. The use of lead citrate at high Ph as an electron-opaque stain in electron microscopy. Journal of Cell Biology 17: 208-212. [ Links ]

Rocha LM, Gonçalves-Esteves V, Luizi-Ponzo AP. 2008. Morfologia de esporos de espécies de Polytrichaceae Schwägr. (Bryophyta) do Brasil. Revista Brasileira de Botânica 31: 537-548. [ Links ]

Rodrigues RS, Luizi-Ponzo AP. 2015. Palinologia de espécies selecionadas da família Pottiaceae (Bryophyta). Pesquisas, Botânica 67: 303-317. [ Links ]

Rohlf J, Fisher DR. 1968. Tests for hierarchical structure in random data sets. Systematic Zoology 17: 407-412. [ Links ]

Savaroğlu F. 2015 Spore morphology of some Orthotrichaceae Arn. species (Bryophyta) from Turkey. Bangladesh Journal of Botany 44: 499-506. [ Links ]

Savaroğlu F, Erkara IP, Koyuncu O. 2017. Observations of spore morphology of some species of Hypnaceae Schimp. (Bryophyta) in Turkey. Bangladesh Journal of Botany 46: 9-17. [ Links ]

Schiffner, V. 1900. Die Hepaticae der Flora von Buitenzorg. I. Leiden, Band. E. J. Brill. [ Links ]

Schuster RM. 1959. A monograph of the Nearctic Plagiochilaceae. Part. I. Introduction and section I. Asplenioides. American Midland Naturalist 62: 1-166. [ Links ]

Schuster RM. 1960. A monograph of the Nearctic Plagiochilaceae. Part. III. Sectio Contiguae to conclusion. American Midland Naturalist 63: 1-130. [ Links ]

Schuster RM. 1980. The Hepaticae and Anthocerotae of North America. IV. New York, Columbia University Press. [ Links ]

Silva-e-Costa JC. 2015. Palinologia de Frullaniaceae Lorch (Marchantiophyta) do Brasil. MSc Thesis. Universidade Federal de Juiz de Fora, Juiz de Fora. [ Links ]

Silva-e-Costa JC, Luizi-Ponzo AP, Resende CF, Peixoto PHP. 2017. Spore germination, early development and some notes on the effects of in vitro culture medium on Frullania ericoides (Nees) Mont. (Frullaniaceae, Marchantiophyta). Acta Botanica Brasilica 31: 19-28 [ Links ]

Silveira M. 2007. Preparo de amostras para Microscopia Eletrônica de Varredura. In: Souza W. (eds.) Técnicas de microscopia eletrônica aplicadas às ciências biológicas. Rio de Janeiro, Sociedade brasileira de microscopia e microanálise. p 47-59. [ Links ]

So ML. 2001. Plagiochila (Hepaticae, Plagiochilaceae) in China. Monographs in Systematic Botany 60: 1-214. [ Links ]

So ML, Grolle R. 2000. Checklist of Plagiochila (Hepaticae). Journal of the Hattori Botanical Laboratory 88: 199-243. [ Links ]

Söderström L, Crandall-Stotler B, Stotler RE, Váňa J, Hagborg A, Konrat M. 2013. Notes on Early Land plants today. 36. Generic treatment of Lophocoleaceae (Marchantiophyta). Phytotaxa 97: 36-43. [ Links ]

Söderström L, Hagborg A, Konrat M, et al. 2016. World checklist of hornworts and liverworts. PhytoKeys 59: 1-828. [ Links ]

Sokal RR, Rohlf J. 1995. Biometry: the principles and practice of statistics in biological research. 3rd. edn. New York, W.H. Freeman and Company. [ Links ]

Spruce R. 1885. Hepaticae amazonicae et andinae. II. Transactions and Proceedings of the Botanical Society of Edinburgh 15: 309-588. [ Links ]

Steinkamp MP, Doyle WT. 1979. Spore wall ultrastructure in four species of the liverwort Riccia. American Journal of Botany 66: 546-556. [ Links ]

Stephani F. 1902. Species hepaticarum 2. Bulletin de l’Herbier Boissier, Série 2 2: 657-688. [ Links ]

Thiers B. [continuously updated]. 2018. Index Herbariorum: A global directory of public herbaria and associated staff. New York, New York Botanical Garden's Virtual Herbarium. [ Links ]

Vojtkó A. 1993. The spore morphology of Hepaticae species. Acta Biologica Szegediensis 39: 59-69. [ Links ]

Wallace S, Fleming A, Wellman CH, Beerling DJ. 2011. Evolutionary development of the plant spore and pollen wall. AoB PLANTS 2011(1). plr027. doi:10.1093/aobpla/plr027 [ Links ]

Wellman CH. 2004. Origin, function and development of the spore wall in early land plants. In: Hemsley AR, Poole I. (eds.) The evolution of plant physiology: From whole plants of ecosystems. London, Linnean Society of London, Paleobotany Specialist Group Elsevier Academic Press. p. 43-60. [ Links ]

Wodehouse RP. 1935. Pollen grains: Their structure, identification and significance in science and medicine. New York, McGraw-Hill. [ Links ]

Yano O, Luizi-Ponzo AP. 2006. Chonecolea doellingeri (Chonecoleaceae, Hepaticae), taxonomia e distribuição geográfica no Brasil. Acta Botanica Brasilica 20: 783-788. [ Links ]

Yano O, Luizi-Ponzo AP. 2011. Dumortiera hirsuta (Dumortieraceae, Marchantiophyta), taxonomy, palynology and geographic distribution. Boletim do Instituto de Botânica 21: 9-18. [ Links ]

Zhao DP, Bai XL, Wang LH. 2011. Observations of spore morphology of some hepatics species (Marchantiophyta) in China. Arctoa 20: 205-210. [ Links ]

Received: November 28, 2018; Accepted: January 30, 2019

* Corresponding author:

Creative Commons License This is an open-access article distributed under the terms of the Creative Commons Attribution License