Rcdisterona de Pfaffia tuberosa (Spreng.) Hicken

Ecdysterone from Pfaffia tuberosa (Spreng.) Hicken

ORIGINAIS

Ecdisterona de Pfaffia tuberosa (Spreng.) Hicken

Ecdysterone from Pfaffia tuberosa (Spreng.) Hicken

Nobushigue Nishimoto^I; Yoshinori Shiobara^I; Shun-suke Inoue^I; Masumi Fujino^I; Tsunematsu Takemoto^I; Cheow Lin Yeoh^II; Goro Hashimoto^III

^IFaculty of Pharmaceutical Sciences, Tokushima Bunri University, Ya­mashirocho, Tokushima-770, Japan

^IIJapan Bio Institute Co., Ltd., Minami-oi, Shinagawa-ku, Tokyo 140, Japan

^IIICentro de Pesquisas de História Natural - Rua Potsdam, 67- Wandar -05318 - São Paulo, SP - Brasil

RESUMO

As raízes secas da espécie brasileira Pfaffia tuberosa (Spreng.) Hicken foram submetidas a extração com metanol. Deste extrato metanólico foram isolados a ecdisterona e o ácido oleanólico, através de processos de separação por HPLC e cromatografia em coluna. Tanto a ecdisterona como o ácido oleanólico foram identificados comparativamenve com os respec­tivos padrões. Análise quantitativa por HPLC mostrou 0,23% de ecdisterona. Este teor foi corsiderado baixo em relação a outra espécie, também brasileira, a Pfaffia iresinoides.

Unitermos:Pfaffia tuberosa (Spreng.) Hicken; ecdisterona; ácido oleanólico.

ABSTRACT

In this paper, we wish to report the isolation and identification of ecdysterone from Pfaffia tuberosa (Spreng.) Hicken. The roots of Pfaffia tuberosa (Spreng.) Hicken were colected in the Mato Grosso State - Brazil. The dried and sliced roots (197g) were extracted with hot MeOH. A suspension of the resulting MeOH extract in the water was treated with n-BuOH. The n-BuOH layer was concentrated in vacuo to give a brown viscous oil (5g), wich was chromatographed on silicagel (65g) using CHCl₃ - MeOH - H2O (80:20:10) lower phase, and 15ml fractions were collected. Fractions 11-13 were concentrated and the residual solid was recrystallized from EtOH to afford oleanolic acid (65mg). The crystalline residue obtained from fractions 44-56 was recrystallized from EtOAC-MeOH to give ecdysterone (87mg). Each compound was identified by direct comparison with an authentic sample. Further HPLC quantitative analysis showed lower content of ecdysterone (0,23%) compared with that of Pfaffia iresinoides Spreng.

Key words:Pfaffia tuberosa (Spreng.) Hicken; ecdysterone; oleanolic acid.

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Agradecimento

Nosso sincero agradecimento a AMERICA LATINA NATURAL, Indústria e Comércio Ltda., São Paulo - Brasil pela colaboração na coleta deste mate­rial para estudo.

• 1 - ARENAS, P. & AZORERO, M.R. Plant used as means of absortion, contra ception, sterilization and fecundation by Paraguayan Indigenous Pepple. Economic Botany, 31:302-6, 1977.
• 2 - NAKAI, N.; TAKAGI, N.; MIICHI, H.; HAYASHI, S.; NISHIMOTO, N.; TAKEMOTO, T.; KIZU, H. Pfaffosides, nortriterpenoid sanins from Pfaffia paniculata. Phytochemistry, 23(8):1703-5, 1984.
• 3 - NISHIMOTO, N.; NAKAI, S.; TAKAGI, N.; HAYASHI, S.;TAKEMOTO,T.; ODASHIMA, S.; KIZU, H.;WADA,Y.  Pfaffosides and nortriterpenoid saponins from Pfaffia paniculata. Phytochemistry, 23(1):139-42, 1984.
• 4 - NISHIMOTO, N.; SHIOBARA, Y.; FUJINO, M.; INOUE, S.; TAKEMOTO, T.; OLIVEIRA, F.; AKISUE, G.; AKISUE, M.K.; HASHIMOTO, G.; TANAKA, O.;KASAI, R.; MATSUURA, H. Ecdysteroids from Pfaffia iresinoides and ressignment of some ¹³CNMR chemical shifts. Phytochemistry, 26(9):2505-7, 1987.
• 5 - OLIVEIRA, F.; AKISUE, G.; AKISUE, M.K. Contribuiçăo para o estudo farmacognóstico do "Ginseng brasileiro" Pfaffia paniculata (Martius) Kuntze. An. Farm. Quim. S.Paulo, 20(1/2):261-77, 1980.
• 6 - TAKEMOTO, T.; NISHIMOTO, N.; NAKAI, S.; TAKAGI, N.; HAYASHI, S.; ODASHIMA, S.; WADA, Y. Pfaffic acid, a novel nortriterpene from Pfaffia paniculata (Martius) Kuntze. Tetrahedron Letters, 24(10):1057-60; 1983.

Datas de Publicação

• Publicação nesta coleção
  18 Mar 2010
• Data do Fascículo
  Dez 1986