Effect of barbatimão [Stryphnodendron adstringens (Mart.) Coville] infusion on the labling of blood elements with technetium-99m

Costa, Thadeu E. M. Maramaldo; Dias, Aline P. Muzy; Capriles, Priscila V. S. Zabala; Oliveira, Márcia Betânia N.; Amorim, Elba Lúcia Cavalcanti de; Lima, Cláudia Sampaio de Andrade; Bernardo-Filho, Mario

doi:10.1590/S0102-695X2002000300004

Abstract

The Barbatimão is a tree which bark is rich in tannin. It is used on popular medicine as a wound healing agent, in the treatment of gastric lesions, as anti-leishmanial agent and as anti-inflammatory. Red blood cells (RBC) are labeled with technetium-99m (Tc-99m) and are utilized in many procedures in nuclear medicine. Some authors have reported that drugs (natural and synthetic) can alter the labeling of RBC with Tc-99m. This study evaluates the effect of barbatimão infusion on the labeling of red blood cells (RBC) and plasma (P) proteins with Tc-99m. Heparinized blood from Wistar rats was incubated with NaCl 0.9% as control and different concentrations of barbatimão infusion. Following the addition of stannous chloride (SnCl2), as reducing agent, and Tc-99m, as sodium pertechnetate, the blood samples were centrifuged. P and RBC were separated and were also precipitated with trichloroacetic acid 5%. Insoluble (IF-P and IF-RBC) fractions were isolated. The percentage of radioactivity in all the samples was determined. All the barbatimão infusion concentrations decreased the labeling of RBC, IF-P and IF-RBC. We can speculate that the barbatimão infusion interfered on the labeling of RBC due to the redox properties and/or it can also act as a chelator of the stannous ion.

Effect of barbatimão [Stryphnodendron adstringens (Mart.) Coville] infusion on the labling of blood elements with technetium99m.

Thadeu E. M. Maramaldo Costa^I,II; Aline P. Muzy Dias^I,II; Priscila V. S. Zabala Capriles^I,II; Márcia Betânia N. Oliveira^I; Elba Lúcia Cavalcanti de Amorim^III; Cláudia Sampaio de Andrade Lima^III; Mario Bernardo-FilhoI,^* * bernardo@uerj.br

^IDepartamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Av. 28 de setembro, 87, 20551-030, Rio de Janeiro, RJ, Brasil

^IIUniversidade Federal do Rio de Janeiro

^IIIUniversidade Federal de Pernambuco, Brasil

ABSTRACT

The Barbatimão is a tree which bark is rich in tannin. It is used on popular medicine as a wound healing agent, in the treatment of gastric lesions, as anti-leishmanial agent and as anti-inflammatory. Red blood cells (RBC) are labeled with technetium-99m (Tc-99m) and are utilized in many procedures in nuclear medicine. Some authors have reported that drugs (natural and synthetic) can alter the labeling of RBC with Tc-99m. This study evaluates the effect of barbatimão infusion on the labeling of red blood cells (RBC) and plasma (P) proteins with Tc-99m. Heparinized blood from Wistar rats was incubated with NaCl 0.9% as control and different concentrations of barbatimão infusion. Following the addition of stannous chloride (SnCl2), as reducing agent, and Tc-99m, as sodium pertechnetate, the blood samples were centrifuged. P and RBC were separated and were also precipitated with trichloroacetic acid 5%. Insoluble (IF-P and IF-RBC) fractions were isolated. The percentage of radioactivity in all the samples was determined. All the barbatimão infusion concentrations decreased the labeling of RBC, IF-P and IF-RBC. We can speculate that the barbatimão infusion interfered on the labeling of RBC due to the redox properties and/or it can also act as a chelator of the stannous ion.

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1 Audi EA, Toledo DP, Peres PG, Kimura E, Pereira WK, de Mello JC, Nakamura C, Alves-do-Prado W, Cuman RK, Bersani-Amado CA. Gastric antiulcerogenic effects of Stryphnodendron adstringens in rats. Phytotherapy Research 1999; 3:264-6
2 Jorge AS, Silveira TGV, Arraes SMAA, Mello JCP, Bertolini DA. Anti-leishmanial "barbatimăo" (Stryphnodendron adstringens (Martius) Coville, Mimosaceae) extract against promastigotes forms of Leishmania (Leishmania) amazonensis. Memórias do Instituto Oswaldo Cruz 1996 (Supplement); 91: 116-20
3 Lima JCS, Martins DTO, Souza PT. Experimental evaluation of stem bark of Stryphnodendron adstringens (Mart.) Coville for anti-inflammatory activity. Phytotherapy Research 1998; 12: 218-20
4 Mello JCP, Petereit F, Nahrstedt A. A dimeric proanthocyanidin from Stryphnodendron adstringens Phytochemistry 1999; 51: 1105-07
5 Lopes GKB, Schuman HM, Hermes-Lima M. Polyphenol tannic acid inhibits hydroxyl radical formation from Fenton reaction by complexing ferrous ions. Biochemica et Biophysica Acta 1999; 1472: 142-52
6 Pietta PG. Flavonoids as antioxidants. Journal of Natural Products 2000; 63: 1035-42
7 Early PJ, Sodee DB. Principles and Practice of Nuclear Medicine. Mosby -Year Book Inc, Toronto, 1995, 877pp.
8 Saha GB. Fundamentals of Nuclear Pharmacy. Springer-Verlag, New York, 1998, 331pp
9 Gutfilen B, Pellini M, Roure-Neder J, Amarante JLM, Evangelista MG, Fernandes SR, Bernardo-Filho, M. 99mTc labeling white blood cells with a simple technique: clinical application. Annals of Nuclear Medicine. 1994; 8: 85-9
10 Gutfilen B, Marinho JCA, Rozemblum S, Bernardo-Filho M. 99mTc-labeled leucocytes obtained with a suitable technique: the biodistribution study in rabbits. Acta Medica et Biologica. 1993; 41: 193-6
11 Plotkowsky MC, Bernardo-Filho M, Meireles MN, Tournier JM, Puchelle E. Pseudomonas aeruginosa binds to soluble cellular fibronectin. Current Microbiology 1993; 26: 91-5.
12 Bernardo-Filho M, Pires ET, Boasquevisque EM, Hassón-Voloch A. Studies on the incorporation of 99m-Technetium to the platyhelminth Dugesia tigrina Revista di Parassitologia 1993; XLIV: 7-11
13 Bernardo-Filho M, Silva JRM, Reis RJN, Boasquevisque EM, Hassón-Voloch. Conditions for labeling of Schistosoma mansoni cercaria with Technetium-99m. The Journal of Nuclear Biology and Medicine 1992; 36: 56-9
14 Oliveira JF, Braga ACS, Oliveira MBN, Ávila AS, Caldeira-de-Araújo A, Cardoso VN, Bezerra RJAC, Bernardo-Filho M. Assessment of the effect of Maytenus ilicifolia (espinheira santa) extract on the labeling of red blood cells and plasma proteins with Technetium-99m. Journal of Ethnopharmacology 2000; 72: 179-84
15 Oliveira JF, Braga ACS, Ávila AS, Fonseca LMB, Gutfilen B, Bernardo-Filho M. Effect of Thuya occidentalis on the labeling of red blood cells and plasma proteins with Technetium-99m. Yale Journal of Biology and Medicine. 1996; 69: 489-94
16 Braga ACS, Oliveira MBN, Feliciano GD, Reiniger IW, Oliveira JF, Silva CR Bernardo-Filho M. The effect of drugs on the labeling of blood elements with Technetium 99m. Current Pharmaceutical Design 2000; 6: 1179-91
17 Reiniger IW, Oliveira JF, Caldeira-de-Araújo A, Bernardo-Filho M. Effect of Peumus boldus on the labeling of red blood cells and plasma proteins with Technetium-99m. Applied Radiation and Isotopes 1999; 51: 145-9

*

bernardo@uerj.br

Publication Dates

Publication in this collection
05 Oct 2009
Date of issue
2002

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

[1] 1 Audi EA, Toledo DP, Peres PG, Kimura E, Pereira WK, de Mello JC, Nakamura C, Alves-do-Prado W, Cuman RK, Bersani-Amado CA. Gastric antiulcerogenic effects of Stryphnodendron adstringens in rats. Phytotherapy Research 1999; 3:264-6

[2] 2 Jorge AS, Silveira TGV, Arraes SMAA, Mello JCP, Bertolini DA. Anti-leishmanial "barbatimăo" (Stryphnodendron adstringens (Martius) Coville, Mimosaceae) extract against promastigotes forms of Leishmania (Leishmania) amazonensis. Memórias do Instituto Oswaldo Cruz 1996 (Supplement); 91: 116-20

[3] 3 Lima JCS, Martins DTO, Souza PT. Experimental evaluation of stem bark of Stryphnodendron adstringens (Mart.) Coville for anti-inflammatory activity. Phytotherapy Research 1998; 12: 218-20

[4] 4 Mello JCP, Petereit F, Nahrstedt A. A dimeric proanthocyanidin from Stryphnodendron adstringens Phytochemistry 1999; 51: 1105-07

[5] 5 Lopes GKB, Schuman HM, Hermes-Lima M. Polyphenol tannic acid inhibits hydroxyl radical formation from Fenton reaction by complexing ferrous ions. Biochemica et Biophysica Acta 1999; 1472: 142-52

[6] 6 Pietta PG. Flavonoids as antioxidants. Journal of Natural Products 2000; 63: 1035-42

[7] 7 Early PJ, Sodee DB. Principles and Practice of Nuclear Medicine. Mosby -Year Book Inc, Toronto, 1995, 877pp.

[8] 8 Saha GB. Fundamentals of Nuclear Pharmacy. Springer-Verlag, New York, 1998, 331pp

[9] 9 Gutfilen B, Pellini M, Roure-Neder J, Amarante JLM, Evangelista MG, Fernandes SR, Bernardo-Filho, M. 99mTc labeling white blood cells with a simple technique: clinical application. Annals of Nuclear Medicine. 1994; 8: 85-9

[10] 10 Gutfilen B, Marinho JCA, Rozemblum S, Bernardo-Filho M. 99mTc-labeled leucocytes obtained with a suitable technique: the biodistribution study in rabbits. Acta Medica et Biologica. 1993; 41: 193-6

[11] 11 Plotkowsky MC, Bernardo-Filho M, Meireles MN, Tournier JM, Puchelle E. Pseudomonas aeruginosa binds to soluble cellular fibronectin. Current Microbiology 1993; 26: 91-5.

[12] 12 Bernardo-Filho M, Pires ET, Boasquevisque EM, Hassón-Voloch A. Studies on the incorporation of 99m-Technetium to the platyhelminth Dugesia tigrina Revista di Parassitologia 1993; XLIV: 7-11

[13] 13 Bernardo-Filho M, Silva JRM, Reis RJN, Boasquevisque EM, Hassón-Voloch. Conditions for labeling of Schistosoma mansoni cercaria with Technetium-99m. The Journal of Nuclear Biology and Medicine 1992; 36: 56-9

[14] 14 Oliveira JF, Braga ACS, Oliveira MBN, Ávila AS, Caldeira-de-Araújo A, Cardoso VN, Bezerra RJAC, Bernardo-Filho M. Assessment of the effect of Maytenus ilicifolia (espinheira santa) extract on the labeling of red blood cells and plasma proteins with Technetium-99m. Journal of Ethnopharmacology 2000; 72: 179-84

[15] 15 Oliveira JF, Braga ACS, Ávila AS, Fonseca LMB, Gutfilen B, Bernardo-Filho M. Effect of Thuya occidentalis on the labeling of red blood cells and plasma proteins with Technetium-99m. Yale Journal of Biology and Medicine. 1996; 69: 489-94

[16] 16 Braga ACS, Oliveira MBN, Feliciano GD, Reiniger IW, Oliveira JF, Silva CR Bernardo-Filho M. The effect of drugs on the labeling of blood elements with Technetium 99m. Current Pharmaceutical Design 2000; 6: 1179-91

[17] 17 Reiniger IW, Oliveira JF, Caldeira-de-Araújo A, Bernardo-Filho M. Effect of Peumus boldus on the labeling of red blood cells and plasma proteins with Technetium-99m. Applied Radiation and Isotopes 1999; 51: 145-9