Acessibilidade / Reportar erro

Ensaio para detectar bergapteno na casca e no caule de Brosimum gaudichaudii Trec através da produção de melanina em actinomicetos

A new assay for detection of furocoumarins in Brosimum gaudichaudii Trec by melanin production in actinomycetes

Resumo

Plant extracts containing furocoumarins compounds were identified by using melanin producer strains of Nocardiopis sp (DAUFPE-361) and non producer strains of Streptomyces sp (DAUFPE-87). Ethanol, methanol and acetone extracted compounds from conduru (Brosimum gaudichaudii) bark and stem were evaporated and added to tubes containing ISP7 medium (tyrosine agar). This medium was inoculated with spore suspension (10(6)-10(7) CFU) prepared from the different actinomycetes strains. The ethanol fraction (pH 9.0) from both bark and stem induced pigmentation in actinomycetes melanin non producer strains and increased the pigmentation in producer strains indicating presence of furocoumarins in the correspondent plant extracts.


Ensaio para detectar bergapteno na casca e no caule de Brosimum gaudichaudii Trec através da produção de melanina em actinomicetos

A new assay for detection of furocoumarins in Brosimum gaudichaudii Trec by melanin production in actinomycetes

M.L.P. Neves* * janetemagali@zipmail.com.br ; P.G. Ferreira Neto; S.M. Souza da Silva; J.M Araújo

Departamento de Antibióticos, Universidade Federal de Pernambuco, Recife, PE, Brasil

ABSTRACT

Plant extracts containing furocoumarins compounds were identified by using melanin producer strains of Nocardiopis sp (DAUFPE-361) and non producer strains of Streptomyces sp (DAUFPE-87). Ethanol, methanol and acetone extracted compounds from conduru (Brosimum gaudichaudii) bark and stem were evaporated and added to tubes containing ISP7 medium (tyrosine agar). This medium was inoculated with spore suspension (106-107 CFU) prepared from the different actinomycetes strains. The ethanol fraction (pH 9.0) from both bark and stem induced pigmentation in actinomycetes melanin non producer strains and increased the pigmentation in producer strains indicating presence of furocoumarins in the correspondent plant extracts.

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

  • 1 Bourgaud, F., Allard, N., Guckert, A. and Forlot, P. Natural sources for furocoumarins. In: Psoralens, past, present and future of photochemoprotection and other biological Activitities .219-230, 1989
  • 2 Pathak, M.A. and Fitzpatrick, T.B. The evolution of photochemotherapy with psoralens and UVA (PUVA): 2000 BC to 1992 AD. J. Photochem. Photobiol. 14B, 3-22, 1992
  • 3 Sampaio S.A.P., Castro, R.M. and Rivitti, E.A. Discromias. Dermatologia Básica 3.ed. 16, 211-221, 1989
  • 4 Shu, C.K. Walradt, J.P. and Tylor, W.J. Improved method for bergapten determination by high-performance liquid chromatography. J. Chromatogr. 106, 271-282, 1975
  • 5 Vande Casteele, K. and Van Sumere, C.F. Separation of phenolics and coumarins by reversed-phase high-performance liquid chromatography. J. Chromatogr. 258, 111-124, 1983
  • 6 Thompson, H. J. and Brown, S.A. Separations of some coumarins of high plants by liquid chromatography. J. Chromatogr. 314, 323-336. 1984
  • 7 Rodighiero, G. and Dall'Acqua, F. Biochemical and medical aspects of psoralens. Photochem. Photobiol. 24, 647-653, 1976
  • 8 Daniels, F.A. Simple microbiological method for demonstrating phototoxic compounds. J. Invest. Dermatol. 32, 259-263, 1965
  • 9 Bourgard, F., Grimal, J.Y., Nguyen, C., Bitton, G. and Guckert, A. A selective photobiological assay to detect and quantify psoralen in psoralea plants (leguminosae). Phytochemical Analysis. 5, 315-318, 1994
  • 10 Shirling, E.B. and Gottlieb, D. Methods for characterization of Streptomyces species. International J of Systematic bacteriology. Washington, v. 16 (3), p. 313 - 340, 1966
  • *
  • Datas de Publicação

    • Publicação nesta coleção
      05 Out 2009
    • Data do Fascículo
      2002
    Sociedade Brasileira de Farmacognosia Universidade Federal do Paraná, Laboratório de Farmacognosia, Rua Pref. Lothario Meissner, 632 - Jd. Botânico, 80210-170, Curitiba, PR, Brasil, Tel/FAX (41) 3360-4062 - Curitiba - PR - Brazil
    E-mail: revista@sbfgnosia.org.br