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Revista Brasileira de Farmacognosia

Print version ISSN 0102-695X

Rev. bras. farmacogn. vol.12  supl.1 Maringá  2002

http://dx.doi.org/10.1590/S0102-695X2002000300035 

Inibidores de proteases encontrados em sementes de Caesalpinia echinata (paubrasil) – isolamento e caracterização do inibidor de tripsina

 

 

Lúcio Garcia de Oliveira; Andrezza Justino Gozzo; Viviane Abreu Nunes; Ilana Cruz Silva; Misako Uemura Sampaio; Claudio Augusto Machado Sampaio; Mariana da Silva Araújo*

Departamento de Bioquímica, Universidade Federal de São Paulo, Rua Três de Maio, 100, 04044-020, São Paulo, SP, Brasil

 

 


RESUMO

Caesalpinia echinata, o pau-brasil, é uma árvore pertencente à família das Leguminosas, sub-família Caesalpinoidae. Como já foram encontrados inibidores de proteases em sementes de outras Leguminosas, o objetivo do trabalho é purificar e caracterizar o inibidor de tripsina extraído das sementes de C. echinata. Após extração salina e precipitação por acetona, os inibidores foram purificados por cromatografia de troca iônica e filtração em gel, apresentando massas moleculares de 19,5 e 10 kDa e constante de inibição da ordem de nM.


 

 

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

 

 

Afgradecimentos

FAPESP, CNPq e FADA-UNIFESP/EPM.

 

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* mariana.bioq@epm.br

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