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Revista Brasileira de Farmacognosia

Print version ISSN 0102-695X

Rev. bras. farmacogn. vol.12  supl.1 Maringá  2002

http://dx.doi.org/10.1590/S0102-695X2002000300041 

Alternative extraction of alkaloid anticarcinogens from Brazilian "vinca rosea" using Ion exchange chromatography

 

 

Sérgio Freire de CarvalhaesI, *; Daniela Lucena CostaII; José Luiz MazzeiIII; Luiz Eduardo Martins TaddeiIII; Luiz Antonio d'AvilaIII

IDepartamento de Química Inorgânica, Instituto de Química, Centro de Tecnologia, Universidade Federal do Rio de Janeiro, 21949-900, Rio de Janeiro, RJ
IIFaculdade de Farmácia, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ
IIIDepartamento de Processos Orgânicos, Escola de Química, Centro de Tecnologia, Universidade Federal do Rio de Janeiro, 21949-900, Rio de Janeiro, RJ, Brazil

 

 


ABSTRACT

Extracts in ethanol and ethanol-ammonia of dried leaves from Catharanthus roseus, gathered at Rio de Janeiro state, were adsorbed in a strongly acidic cation exchange resin with sulfonic acid group, using the finite bath method, resulting in an alkaloid retained fraction and an acidic and neutral unretained fraction. High Performance Liquid Chromatography showed the isolation of the alkaloid fraction to be highly selective and with good performance, with an absence of alkaloids in the unretained fraction, while the retained fraction presented 1,54-6,35 mg/g of vindoline and 0,12-0,91 mg/g of vinblastine, common for an alkaloid-rich concentrate, usually obtained by classic extraction with several steps using solvents.


 

 

Full text available only in PDF format.

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Acknowledgments

The authors gratefully acknowledge the support received from Fundação Universitária José Bonifácio (FUJB/UFRJ) (Processo 8012-5), Conselho Nacional para o Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and Bayer S.A.

 

References

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* sfcarv@iq.ufrj.br

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