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Revista Brasileira de Farmacognosia

Print version ISSN 0102-695X

Rev. bras. farmacogn. vol.12  supl.1 Maringá  2002

http://dx.doi.org/10.1590/S0102-695X2002000300055 

Dosagem de artemisinina em Artemisia annua L. por cromatografia líquida de alta eficiência com detecção por índice de refração

 

 

Vera Lúcia Garcia RehderI,*; Marili Villa Nova RodriguesI; Adilson SartorattoI; Mary Ann FoglioII

IDivisão de Química Orgânica e Farmacêutica do CPQBA, UNICAMP, Paulínia, SP
IIDivisão de Fitoquímica do CPQBA, UNICAMP, Paulínia, SP

 

 


ABSTRACT

Artemisinin is a sesquiterpene lactone used in treatment of chloroquine-resistant malaria. This paper presents high-performance liquid chromatographic assay for artemisinin in leaves of A. annua using differential refractometer detector and a single step of clean-up in a silica cartridge. The average of recoveries were 95% and the limit of quantification was 0,21% p/p using 200 mg of the leaves. This method was found to be simple, robust and relatively rapid.


 

 

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Referências

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5 Delabays N. Biologie de la reproduction chez L'Artemisia annua L. et genetique de la production em artemisinine, Universite de Lausanne, France, Thèse de Doctorat., 1997        [ Links ]

6 Elsohly H N, Croom E M, Elesohly M A. Analysis of the antimalarial sesquiterpene artemisinin in Artemisia annua by high-performance liquid chromatography with postcolumn derivatization and ultraviolet detection. Pharmaceutical Research. 1987; 4: 258-60        [ Links ]

 

 

* rehder@cpqba.unicamp.br

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