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Revista Brasileira de Farmacognosia

Print version ISSN 0102-695X

Rev. bras. farmacogn. vol.12  supl.1 Maringá  2002 

Dosagem de artemisinina em Artemisia annua L. por cromatografia líquida de alta eficiência com detecção por índice de refração



Vera Lúcia Garcia RehderI,*; Marili Villa Nova RodriguesI; Adilson SartorattoI; Mary Ann FoglioII

IDivisão de Química Orgânica e Farmacêutica do CPQBA, UNICAMP, Paulínia, SP
IIDivisão de Fitoquímica do CPQBA, UNICAMP, Paulínia, SP




Artemisinin is a sesquiterpene lactone used in treatment of chloroquine-resistant malaria. This paper presents high-performance liquid chromatographic assay for artemisinin in leaves of A. annua using differential refractometer detector and a single step of clean-up in a silica cartridge. The average of recoveries were 95% and the limit of quantification was 0,21% p/p using 200 mg of the leaves. This method was found to be simple, robust and relatively rapid.



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1 Who (World Health Organization), 1998. "Malaria." Fact Sheet nº 94. 10/1998, (01/09/2000)        [ Links ]

2 Klayman DL. Qinghaosu (artemisinin): an antimalarial drug from China. Science. 1985;.228:1049-55        [ Links ]

3 Ferreira J F S & Janick J. Distribuition of artemisinin in Artemisia annua. IN: Progress in new crops. In: Janick J, editor. ASGS Press, Arlington. 1996; 579-84        [ Links ]

4 Elhag H M, El-Doiaty M M, El-Feraly F S, Mossa J S, El-Olemy M M. Selection and micropropagation of high artemisinin producing clones of Artemisia annua L. Phytotherapy Research. 1992; 6: 20-4        [ Links ]

5 Delabays N. Biologie de la reproduction chez L'Artemisia annua L. et genetique de la production em artemisinine, Universite de Lausanne, France, Thèse de Doctorat., 1997        [ Links ]

6 Elsohly H N, Croom E M, Elesohly M A. Analysis of the antimalarial sesquiterpene artemisinin in Artemisia annua by high-performance liquid chromatography with postcolumn derivatization and ultraviolet detection. Pharmaceutical Research. 1987; 4: 258-60        [ Links ]




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