SciELO - Scientific Electronic Library Online

vol.12 suppl.1Dosagem de artemisinina em Artemisia annua L. por cromatografia líquida de alta eficiência com detecção por índice de refraçãoAlcalóides isolados Hornschuchia obliqua (Annonaceae) author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Revista Brasileira de Farmacognosia

Print version ISSN 0102-695X

Rev. bras. farmacogn. vol.12  supl.1 Maringá  2002 

Preparation and activity of diterpenoids against trypomastigotes of Trypanosoma cruzi



Jacqueline A. TakahashiI,*; Henriete S. VieiraI; Eliane A. SilvaI; Maria A. D. BoaventuraI; Alaíde B. de OliveiraII; Egler ChiariIII

IDepartamento de Química, ICEx, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, 31270-901, Belo Horizonte, MG, Brazil
IIDepartamento de Produtos Farmacêuticos, Faculdade de Farmácia, UFMG, Av. Olegário Maciel, 2360, 30180-112, Belo Horizonte, MG
IIIDepartamento de Parasitologia, ICB, UFMG, Av. Antônio Carlos, 6627, CP 2486, 31270-901, Belo Horizonte, MG




A systematic investigation on the trypanocidal effect of several natural products isolated from Brazilian plant species has been carried out. In this paper we report on the results obtained from the screening of 26 diterpenes from natural sources or of synthetic/microbial transformations origin (mainly derivatives of kaurenoic acid) against trypomastigote forms of Trypanosoma cruzi, the causative agent of Chagas'disease. In the in vitro assays, kaurenoic acid, kaurenol, acutifloric acid and stemodin showed a complete elimination of parasites from the blood. Therefore, such diterpenoids can be considered as starting materials for molecular modification in the search for lead compounds for clearance of infected blood to be used in transfusions. Blood previously treated with active compounds was submitted to an infectivity test. Samples proceeded from treatment with kaurenol and kaurenoic acid showed to be completly clean from T. cruzi as no infection was observed in mice inoculated with contaminated blood treated by these compounds.



Full text available only in PDF format.

Texto completo disponível apenas em PDF.




The authors thank CNPq and FAPEMIG (Brazil) for fellowships and financial support.



1 Hammond DJ, Hogg L, Gutteridge WE. Trypanosoma cruzi: Possible Control of Parasite Transmission by Blood Transfusion Using Amphiphilic Cationic Drugs. Experimental Parasitology. 1985; 60:32-42        [ Links ]

2 Ghisalberti EL. The Biological Activit of Naturally Occurring Kaurane Diterpenes. Fitoterapia 1997; LXVIII: 303-325        [ Links ]

3 Alves TMA, Chaves PPG, Santos LMST, Nagem TJ, Murta SMF, Geravolo IP, Romanha AJ, Zani CL. A Diterpene from Mikania obtusata Active on Trypanosoma cruzi. Planta Med. 1995; 61: 85-87        [ Links ]

4 Roque NF, Giannella TL, Giesbrecht AM, Barbosa RCSBC. Kaurene Diterpenes from Wedelia paludosa. Rev. Latinoam. Quim. 1987; 18: 110-111        [ Links ]

5 Urbina JA, Lazard K, Aguirre T, Piras MM, Piras R. Antiproliferative Effects and Mechanism of Action of ICI 195,739, and Novel Bis-triazole Derivative, on Epimastigotes and Amastigotes Forms of Trypanosoma (Schizotrypanum) cruzi. Antimicrob. Agents Chemother. 1991; 35: 730-735        [ Links ]

6 Takahashi JA, Boaventura MAD, Bayma JC, Oliveira AB. Frutoic Acid, a Dimeric Kaurane Diterpene from Xylopia frutescens. Phytochemistry. 1995; 40: 607-609        [ Links ]

7 Hanson JR, Hitchcock PB, Takahashi JA. Biotransformation of ent-16b,19-dihydroxykaurane by Cephalosporium aphidicola. Phytochemistry. 1995; 40: 797-800        [ Links ]

8 Boaventura MAD, Hanson J., Hitchcock PB, Takahashi JA. The Biotransformation of ent-19-hydroxykaur-16-en-15-one by Cephalosporium aphidicola. Phytochemistry. 1994; 37:387-389        [ Links ]

9 Oliveira AB, Hanson JR, Takahashi JA. The Biotransformation of ent-15-oxokaur-16-en-19-oic Acid and its Methyl Ester by Cephalosporium aphidicola. Phytochemistry 1995; 40: 439-442         [ Links ]

10 Oliveira AB, Saúde DA, Perry KSP, Duarte DS, Raslan DS, Boaventura MAD, Chiari E. Trypanocidal Sesquiterpenes from Lychnophora Species. Phytotheraphy Research. 1996 10: 292-295        [ Links ]




Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License