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Rev. bras. farmacogn. vol.21 no.3 Curitiba May/June 2011 Epub May 20, 2011
Ane R. T. Costa; Suzana F. Alves; Edemilson C. da Conceição; Clévia F. D. Garrote; José R. Paula; Maria Teresa F. Bara*
Laboratório de Pesquisa em Produtos Naturais, Faculdade de Farmácia, Universidade Federal de Goiás, Brazil
The dissolution test is an essential tool to assess the quality of herbal medicines in the solid dosage form for oral use. This work aimed to evaluate the dissolution behavior of three herbal medicines in the form of capsules and tablet containing Passiflora, produced with powder or dried extract. Assay of total flavonoids and dissolution methods were validated and obtained results allowed the quantification of flavonoids with precision, accuracy and selectivity. The percentage of total flavonoids found was 2% for capsule A (containing only powder), 0.97% for capsule B (containing only dried extract) and 5.5% for tablet. Although the content was lower, the release of flavonoids present in the capsule containing dried extract was 12% higher over 30 min, with dissolved percentage values of 87 and 75, for the capsules containing extract and powder, respectively. The tablet containing dried extract presented dissolution of 76%, despite the higher content of flavonoids, which may be due to pharmacotechnical problems. Obtained data demonstrated the need to implement these tests in the quality control of herbal medicines, confirming the release of the active ingredients that underlie the pharmacological action of these medicines.
Keywords: flavonoids, natural product, Passiflora, passion fruit, quality control
Herbal products are medicines whose active principles are exclusively derived from vegetable drugs. They are characterized by knowledge of the effectiveness and risks of their use, as well as by the reproducibility and constancy of their quality (WHO, 1996; Emea, 2003). Although they are natural products, they are not necessarily innocuous (Bateman et al., 1998; Turolla & Nascimento, 2006).
Dissolution studies represent an indispensable tool for pharmaceutical development, since they make it possible to check whether the formulation is suitable for the purpose for which it is intended (Sittichai et al., 2007). In the sector of pharmaceutical production and quality control, the dissolution test can be used to detect quality deviations in parameters such as content uniformityand ensure reproducibility between production batches (Marques, 2002; Souza et al., 2007).
In order to have a therapeutic effect, it is necessary for the oral dosage form to dissolve in the appropriate section of the digestive system. After that, its active principles can be absorbed thus reaching the circulation and gaining access to sites of action. Therefore, the evaluation of the dissolution profile reveals the portion of the herbal product that is actually available for absorption in the gastrointestinal tract and that will reach the circulation. In terms of pharmacokinetics, absorption of a drug is controlled by its dissolution, so that different dissolution profiles indicate different bioavailabilities, which can lead to variations in the therapeutic and toxic effect of the product used (Ngo, 2007).
There have been few published studies on the dissolution of herbal products, and the data reported are worrying in terms of variations in the release of active principles, associated with the processing of raw material, particle size of dried extracts, moisture, and compression force, among other factors (Bempong & Houghton, 1992; Taglioli et al., 2001; Kressmann et al., 2002; Westerhoff, et al., 2002; Kratz et al., 2008).
Given the above, the dissolution test of capsules and tablet based on Passiflora sp. is important due to it being widely consumed by the population and also because of the lack of this kind of study involving herbal medicines. In this work, methods for the quantification and release of total flavonoids were developed and validated to evaluate the dissolution behavior of capsules and tablet containing powder or dried extract of Passiflora.
Materials and Methods
Boric acid from Vetec (Brazil), ethyl acetate P.A. from CRQ (Brazil), ethanol 95% from F. Maia (Brazil), formic acid anhydrous 99% from Acros Organics (USA), glacial acetic acid P.A. from F. Maia (Brazil), glacial acetic acid P.A. from CRQ (Brazil), hydrochloric acid 37% from F. Maia (Brazil), isovitexin 98% from Fluka (Germany), methanol P.A. from F. Maia (Brazil), natural products (NP): 2-aminoethanol diphenylborate from Sigma (Germany), oxalic acid P.A. from Vetec (Brazil), potassium phosphate monobasic anhydrous P.A. from Synth (Brazil), rutin 95% HPLC grade from Sigma (Brazil), sodium hydroxide P.A. from Biotec (Brazil), and vitexin 96% from Fluka (Germany).
Passiflora powder and dried extract were used as raw materials for the production of capsules. The tablet was randomly purchased in a local market. Dissolution tests were carried out on capsules containing 400 mg of Passiflora sp. powder (capsule A), capsules containing 250 mg of dried extract of Passiflora sp. (capsule B) and tablets containing 320 mg of dried extract of Passiflora incarnata L.
Identification of flavonoids
Flavonoids present in the raw materials, dried extract and tablet were identified by absorption spectra and by thin layer chromatography (TLC) (Wagner & Bladt, 2001). These flavonoids were compared with reference substances vitexin, isovitexin and rutin. For TLC, 1 g of sample was weighed and dissolved in 10 mL of methanol followed by heating in a water bath for 5 min. This solution was filtered and 30 µL were applied to silica gel 60 F254 Chromatoplates Alugram (Germany), 4 x 10 cm in size and with a thickness of 0.25 cm. The reference solutions used were rutin, vitexin and isovitexin, which were solubilized (2 mg) in 10 mL of methanol and then 10 µL were applied to the silica plates. The mobile phase used was ethyl acetate:formic acid anhydrous:glacial acetic acid:water (100:11:11:26). After the chromatographic run, the chromatogram was developed with a solution of 1% natural products (NP) in methanol, followed by visualization under UV illumination at 365 nm.
The moisture content was determined in raw material (powder and dried extract), the average weight of the capsules was measured, and total flavonoids were quantified in the herbal product and in the dissolution medium containing the herbal product (adapted from the British Pharmacopoeia, 2007). A Varian spectrophotometer, model Cary 50, and a Vankel dissolutor model VK 7000, were used.
Quantification of total flavonoids by UV spectrophotometry (adapted from the British Pharmacopoeia, 2007)
The steps taken to quantify the total flavonoids content of Passiflora in raw materials, capsules and tablet, as well as the dissolution medium, are presented in the form of a flowchart in Figure 1. The analysis of total flavonoids content was performed in triplicate. To determine the percentage of total flavonoids (TF%) in the samples a calibration curve was prepared using rutin as the standard (0.05 mg/mL) prepared in ethanol 60% v/v, followed by serial dilution to achieve concentrations ranging from 0.002 mg/mL to 0.012 mg/mL. The same procedure shown in Figure 1 was then performed with the standard.
The total flavonoids content was calculated using the following formula:
in which: TF% = percentage of total flavonoids in the sample; x = concentration of sample according to the calibration curve in mg/mL; DF = dilution factor; w = sample weight in mg.
To analyze the total flavonoids content in capsules and tablet all the steps shown in Figure 1 were carried out, but in the preparation of the samples initially the average weight of capsules and tablets was determined, and for the quantification of flavonoids the equivalent of 200 mg of herbal product were weighed.
To analyze the total flavonoids content in the dissolution medium, aliquots of 50 mL were manually collected from each tank of the dissolutor and filtered, evaporated to complete dryness and then treated according to the procedure shown in Figure 1 (test solution). The percentage of total flavonoids in the dissolution medium (MF%) was calculated as described for calculation of total flavonoids (TF%), adjusting the dilution factor by 500. To calculate the percentage of total dissolved flavonoids (Q), we used the following relationship:
in which Q is the amount of total flavonoids dissolved in relation to the total flavonoid content of herbal products; MF% = percentage of total flavonoids in the dissolution medium; TF% = percentage of total flavonoids in the herbal products analyzed.
Validation of spectrophotometry method
The method of analysis of total flavonoids content in the capsules was validated according to Anvisa (2003). Evaluated parameters were selectivity, linearity and range, precision (repeatability and intermediate), accuracy and robustness.
The selectivity was evaluated from the analysis of the absorption spectra of solutions used in the assay, with the wavelength ranging from 200 to 800 nm. The linearity was evaluated by processing the data by linear regression of three calibration curves using rutin as a reference substance at concentrations of 2, 4, 6, 8, 10 and 12 µg/mL. Precision (repeatability) was determined by analyzing six replicates of the sample at the concentration of 100% (weight of 200 mg of sample according to the pharmacopoeic method of quantification). Samples were prepared according to Figure 1 and the results were expressed as relative standard deviation (RSD%). Precision (intermediate) was determined on two days and by two different analysts, in triplicate, at a concentration of 100%. To this end, samples were prepared according to Figure 1, and the results were expressed as relative standard deviation (RSD%). The accuracy was determined by analyzing the sample in triplicate at concentrations of 50%, 100% and 150% of the theoretical concentration analyzed and verified through recovery test. The robustness of the method was evaluated in triplicate by varying the wavelength of the reading (398 nm, 401 nm and 404 nm), the spectrophotometer (equipment) and glacial acetic acid manufacturer. It was expressed as relative standard deviation (RSD%).
Validation of dissolution method
The dissolution test was classified according to its purpose in test category III, hence the need for assessment of the parameter precision, by means of repeatability (Anvisa, 2003). The precision was determined by analysis of six individual samples (six replicates) collected from the dissolutor after 30 min. The repeatability was expressed as the relative standard deviation between samples (RSD%).
The analytical methodology used to evaluate the dissolution followed the USP 30 (2007) guidelines, which describe the general methodology for a dissolution test for tablets and capsules. The test was carried out using 0.1 M HCl as the dissolution medium, a volume of 500 mL, apparatus 2 (paddle), with a stirring speed of 50 rpm, and at a temperature of 37±0.5 ºC. Aliquots of 50 mL were collected manually at 0, 10, 20 and 30 min, filtered and assayed by spectrophotometry at 401 nm after oxalo-boric acid reaction for the assay of total flavonoids, using the equation:
in which Q is the amount of total flavonoids dissolved related to the total flavonoids in the herbal products assay; MF% is the percentage of total flavonoids in the dissolution medium; TF% is the percentage of total flavonoids in the herbal products analyzed.
Results and Discussion
The analysis of the absorption spectra carried out to identify the samples showed absorption peaks in the ultraviolet (UV) region characteristic of flavonoids. Scans performed on methanolic solutions of raw materials showed a peak between 269 nm and 300 nm for powder and a peak at 274 nm for dried extract of Passiflora, and a lower absorbance for both powder and extract in the region between 300 and 400 nm (Figure 2). Because of their chemical structure, flavonoids possess characteristic absorption spectra in the UV region, determined by the benzopyrone core and with two peaks of absorption, one occurring between 240 and 285 nm (band II) and another between 300 and 400 nm (band I) (Zuanazzi & Montanha, 2007).
It should be emphasized that the choice of rutin as a marker in this study was based on its absorption spectrum in the UV region being similar to those of vitexin and isovitexin, which are the main markers of Passiflora. Reference substances vitexin and rutin showed two absorption peaks in the UV region characteristic of flavonoids. Furthermore, after the complexation reaction (flavonoid-reactive) spectra of test solutions prepared with rutin or vitexin and the samples showed peaks of absorption around 401 nm. As the method used was spectrophotometry and not chromatography, this caused no inconvenience in relation to selectivity, besides which the cost of rutin makes it more accessible. A study performed by Taglioli et al. (2001) used another flavonoid, hyperoside, as a marker of Passiflora sp. in dissolution tests, and this was also quantified by spectrophotometry.
The chromatograms obtained by TLC for raw materials, herbal products and reference substances were important to support their identification and showed the presence of three easily visible spots for Passiflora dried extract with retention factors (Rf) of 0.25; 0.56 and 0.69. Passiflora powder exhibited five spots with Rf of 0.31; 0.37; 0.5; 0.62 and 0.69. The reference substances used, vitexin, isovitexin and rutin, showed yellow-greenish spots with Rf of 0.81; 0.69 and 0.5, respectively, when visualized with natural products (NP) (Figure 3). The tablet presented seven spots with Rf of 0.19; 0.25; 0.31; 0.56; 0.62; 0.69; 0.75. The chromatographic profile of these products showed the presence of flavonoids and was similar to the TLC chromatographic profile described in the British Pharmacopoeia (2007) for Passiflora incarnata species.
The moisture content of raw materials (powder = 5.15% and dried extract = 5.7%) and average weight values for herbal products showed that samples were in accordance with these parameters. The total flavonoids found comprised 2% of capsule A (containing powder), 0.97% of capsule B (containing dried extract) and 5.5% of the tablet.
Validation of spectrophotometry method
The results of the validation of the spectrophotometric method for quantification of total flavonoids in the capsules are shown in Table 1. The method was found to be selective, linear, precise, accurate and robust for the analysis of the total flavonoids content of Passiflora sp. under the experimental conditions used.
During the validation study neither the stability of the solution nor the reaction time for formation of the flavonoid-reactive complex was investigated, since the proposed conditions of the pharmacopeic method did not change.
The method was not validated for tablets because it was not possible to identify the matrix (excipients) used by the manufacturer and this could interfere with the analysis, although it is believed that in the process of extraction and reaction with boric and oxalic acid, which is specific for flavonols, such interference was minimal for the tablet in this study. To this end, the use of HPLC would be recommended.
Validation of dissolution method
The method was found to be precise in terms of repeatability for the quantification of flavonoids at 401 nm, with aliquots being removed at 30 min and with an RSD <5% (1.06% for capsules A and 2.16% for capsules B).
The results of dissolution tests of the samples are presented as percentage of drug dissolved during the preset collection time. As observed, the dissolution of the capsules showed that the average percentages of total flavonoids dissolved (Q) were 87±1.56 % for those produced with dried extract and 75±0.794 % for those containing the powder of Passiflora, after the first 10 min of the test, and this remained practically constant over the next 20 min (Figure 4). It is interesting to note that although the capsule containing powder of Passiflora employed in this study presented a higher flavonoids content (2%), the dissolution of the capsules produced with dried extract (flavonoid content: 0.97%) was 12% higher in the final 30 min. This demonstrates the need for standardization of vegetable raw materials used in the production of herbal products and may be an advantage of the use of dried extracts in relation to powder.
Although there is no pharmacopoeia specification for the dissolution of this herbal product, the acceptance criterion used in this study was at least 75% of total flavonoids dissolved after 30 min of testing. On the basis of the results obtained the capsules meet such a specification.
It is important to point out that quick initial release of a drug from its matrix system may be undesirable therapeutically (Jackson et al., 2000). It is necessary to evaluate the release model presented in Figure 4, in which around 40 to 50% of the active principle (presented in powder and dried extract, respectively) was released from the formulation in the first 5 min of the test, and further evaluation through studies of bioequivalence is necessary to determine whether the organism is able to absorb all of the active principle readily released at the beginning of the dissolution process, without affecting its ability to maintain ideal plasma concentrations for the therapeutic performance of products.
Taglioli et al. (2001) demonstrated 50% dissolution for capsules containing powder of Passiflora sp. and close to 100% dissolution for capsules containing freeze-dried extract and a standardized extract (Passiflo2-LMFR) after about 10 min of test with a paddle apparatus, at a stirring speed of 50 rpm, and using water as the dissolution medium at 37±0.5 ºC, with spectrophotometric detection at 340 nm.
The dissolution tests carried out with tablets made from dried extract of P. incarnata gave a Q value of 76%. It can be seen that for the capsule and tablet made from dried extract, although they were prepared from different species of Passiflora, the release of flavonoids was 11% higher for the capsule. The total flavonoids contents were also very different (0.97% for capsule compared to 5.5% for tablet). The differences in the dissolution of these herbal products may have resulted from pharmacotechnical problems such as interaction with excipients in the formulation of tablets, compression force employed in their manufacture, moisture, particle size and various extraction techniques used to obtain the dried extracts (Westerhoff et al., 2002; Calixto, 2000), although none of these factors was investigated in this study.
Finally, it should be emphasized that one of the greatest challenges facing the market for herbal products is the standardization of vegetable raw materials (Capasso et al., 2000; Ong 2004; Liu & Wang, 2008; Alvarenga et al., 2009; Bueno et al., 2009; Valente et al., 2009). New technologies to modernize the preparation of traditional herbs as part of pharmaceutical products are being evaluated with the ultimate goal of maximizing the opportunities and overcoming the challenges inherent to herbal medicines (Vasconcelos et al., 2005; Yadav & Dixit, 2008).
Considering that herbal products represent a market niche and carry the potential to diversify and innovate in the pharmaceutical industry, both in terms of developing new medicines and in improving the quality of those currently available, the implementation of new technologies and processes may contribute to ensuring the efficacy and safety of these products (Alves, 2005). In this context, dissolution studies of herbal medicines may contribute to the process of innovation in the pharmaceutical industry.
The results obtained allow us to suggest the use of the methods employed in this study in the quality control (analysis of content and dissolution tests) of Passiflora capsules, since the methods were validated by demonstrating selectivity, linearity and range, precision (repeatability and intermediate precision), accuracy and robustness.
For the dissolution test of capsules containing Passiflora sp. (powder or dried extract), the following conditions were validated: stirring speed of 50 rpm; apparatus 2, paddle; time for quantification of samples of 30 min; dissolution medium 0.1 M HCl; volume of dissolution medium 500 mL; detection at 401 nm after reaction with oxalic and boric acid.
It was possible to compare the dissolution of capsules containing Passiflora sp. (powder or dried extract) so that those based on dried extract showed ahigher percentage of total flavonoids released into the dissolution medium, although the flavonoids content of herbal products (capsule B) was found to be half that of capsules A (containing powder). We also observed that for both capsules of Passiflora, the maximum dissolution was achieved in the first 10 min of testing.
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Received 10 Apr 2010
Accepted 22 Sep 2010
* Correspondence: Maria Teresa F. Bara, Laboratório de Pesquisa em Produtos Naturais, Faculdade de Farmácia, Universidade Federal de Goiás, Caixa postal 131, 74605-220 Goiânia-GO, Brazi, firstname.lastname@example.org, Tel. +55 62 3209 6182