versão impressa ISSN 0102-695X
Rev. bras. farmacogn. vol.22 no.2 Curitiba mar./abr. 2012 Epub 05-Dez-2011
Luís GasparI; Andreia P. OliveiraI; Luís R. SilvaI; Paula B. AndradeI; Paula Guedes de PinhoII; João BotelhoI; Patrícia ValentãoI, *
IREQUIMTE/Laboratório de Farmacognosia, Departamento de Química, Faculdade de Farmácia, Universidade do Porto, Portugal
IIREQUIMTE/Laboratório de Toxicologia, Departamento de Ciências Biológicas, Faculdade de Farmácia, Universidade do Porto, Portugal
Coreopsis tinctoria Nutt., Asteraceae, flowering tops infusion has been traditionally used in many countries to control hyperglycaemia. In this work we report for the first time fatty acids and volatile compounds in this species. Fifteen fatty acids and sixteen volatile compounds were determined by GC-ITMS, being saturated fatty acids and monoterpenes the main compounds. The antioxidant and antibacterial potential of this matrix was checked for the first time by several in vitro assays. A concentration-dependent activity was noticed against DPPH, nitric oxide and superoxide radicals. Antibacterial capacity was assessed against Gram-positive and Gram-negative bacteria, being more effective against the first. Additionally, acetylcholinesterase and butyrylcholinesterase inhibitory activity was also evaluated, but no effect was found. Our results provide evidence of a wide diversity of compounds with several biological properties, improving the knowledge on this poorly studied matrix, which can lead to an increment of the use of C. tinctoria flowering tops, namely in food and pharmaceutical applications.
Keywords: antibacterial, antioxidant, Coreopsis tinctoria, fatty acids, volatile compounds
The use of plants to treat or prevent several diseases is in the basis of many traditional treatments. Additionally, they are regarded as important sources of essential nutrients and health -beneficial components that are crucial for human life, such as vitamins, minerals, phenolic compounds, volatiles and fatty acids (Grusak, 2002). The importance of fatty acids in plants lies on the fact that they constitute signal molecules, energy storage, and protect plants from environmental and biological stress (Ohlrogge, 1997). In the human organism fatty acids act like hormones or their precursors, help the digestion process and are a source of metabolic energy (Burtis & Ashwood, 1996).
Plants show a constitutive emission of volatile compounds that are released from the surface and/or accumulated in storage sites. These compounds usually have low molecular weight and can be derived from amino acids, fatty acids and carbohydrates (Schwab et al., 2008). Apart from their involvement in insect/plant interaction, volatiles are also important for human health (Dudareva et al., 2004, Schwab et al., 2008). It is well known that some volatiles can protect against oxidation, or cellular damage caused by reactive species, preventing the initiation of several diseases, such as many types of cancer and neurodegenerative illnesses (Wei et al., 2001).
In recent years there has been an increasing search in natural compounds able to inhibit the action of cholinesterases for Alzheimer's disease (AD) treatment. AD is a neurodegenerative disorder of the central nervous system characterized by profound memory impairment, emotional disturbance, and, in late stages, by personality changes (Houghton et al., 2006). Furthermore, the exploitation of novel antimicrobial agents from plants has been of great interest too. Medicinal plants contain a large number of primary and secondary metabolites that are known to retard or inhibit the growth of bacteria, yeasts and molds (Burt & Reinders, 2003; Chorianopoulos et al., 2008; Taveira et al., 2010).
Coreopsis tinctoria Nutt., Asteraceae, is native from North America, but it has spread worldwide. Its flowering tops infusion has been traditionally used in many countries to control hyperglycaemia (D´Oliveira Feijão, 1973). Despite this, C. tinctoria flowering tops constitute a poorly studied matrix. Only recently few studies have correlated its chemical composition with the promotion of glucose tolerance (Dias et al., 2010a; Dias et al., 2010b). So far, C. tinctoria flowering tops have been reported to contain flavonoids, namely chalcones (Shimokoriyama, 1957), aurones (Shimokoriyama, 1957), anthocyanins (Shimokoriyama, 1957), flavanones (Shimokoriyama, 1957) and flavonols (Dias et al., 2010a), as well as phenylpropanoids (Dias et al., 2010a, Shimokoriyama, 1957, Reichling & Thron, 1989).
Thus, this work aims to improve the knowledge on the metabolic profile of this species and on its biological potential. Following these purposes, the determination of fatty acids and volatile compounds was pursued. Additionally, antioxidant capacity, acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) inhibitory capacities and antibacterial activity against Gram-positive and Gram-negative bacteria were evaluated by in vitro assays.
Material and Methods
The dried flowering tops of Coreopsis tinctoria Nutt., Asteraceae, were from a medicinal plants distributor (Morais e Costa & C.ª Lda, Portugal). The identity was checked by the authors following the characteristics described in the Portuguese Flora (Coutinho, 1939). The acquired sample was also compared with an individual occurring in nature. The plant material was powdered (mean particle size lower than 910 µm). Voucher specimen was deposited at Laboratório de Farmacognosia, Faculdade de Farmácia, Universidade do Porto (CTF-LG-102010).
Standards and reagents
All chemicals used were of analytical grade. The standard compounds were purchased from various suppliers: α-thujone, α-pinene, bornyl acetate and benzaldehyde were from Sigma-Aldrich (St. Louis, MO, USA), fatty acid methyl esters kit and camphene were purchased from Supelco (Bellafonte, PA, USA), methyl jasmonate (internal standard), β-caryophyllene, β-caryophyllene epoxide, β-terpineol, cis-carveol, trans-carveol and myrtenal were from SAFC (St. Louis, USA) and borneol, carvone, verbenone, β-linalool and limonene were from Fluka (Buchs, Switzerland). 1,1-Diphenyl-2-picrylhydrazyl (DPPH), β-nicotinamide adenine dinucleotide (NADH), phenazine methosulphate (PMS), nitrotetrazolium blue chloride (NBT), 5,5'-dithio-bis(2-nitrobenzoic acid) (DTNB), sulphanilamide, acetylcholinesterase (type VI-s, lyophilized powder) from electric eel (Electrophorus electricus), acetylthiocholine iodide (ATCI), butyrylcholinesterase from equine serum (lyophilized powder), S-butyrylthiocholine chloride and sodium nitroprusside dehydrate (SNP) were purchased from Sigma-Aldrich (St. Louis, MO, USA). N-(1-Naphthyl)ethylenediamine dihydrochloride, ethanol, potassium dihydrogen phosphate and ciprofloxacin were obtained from Merck (Darmstadt, Germany). Sodium sulphate anhydrous and isooctane were purchased from Panreac Química SA (Barcelona, Spain). Potassium hydroxide was from Pronalab (Lisboa, Portugal) and boron trifluoride (BF3) 10% methanol solution from Supelco (Bellafonte, PA, USA). Mueller Hinton Broth (MHB) and Mueller Hinton Agar (MHA) media were acquired to Liofilchem (Teramo, Italy).
For a broader insight on the chemical composition of the species, two different extracts were assessed.
C. tinctoria dried flowering tops (1 g) was extracted with 200 mL of boiling water for 20 min, with subsequent filtration and freezing. The extract was lyophilized and kept in a desiccator, in the dark. This extract was prepared as this plant is commonly consumed as infusion.
C. tinctoria dried flowering tops (1.5 g) was mixed with chloroform:methanol (2:1) (5×150 mL) with magnetic stirring (500 rpm), for 10 min, at 40 °C. The resulting extract was then filtered through a Buchner funnel, under vacuum. Afterwards the extract was concentrated to dryness under reduced pressure (40 °C).
C. tinctoria aqueous extract (0.1 g) was agitated with chloroform:methanol (2:1) (5×10 mL, 500 rpm), for 10 min, at 40 °C, concentrated to dryness under reduced pressure and redissolved in methanol. The organic extract prepared as described above was also redissolved in methanol. Fatty acids were determined as previously reported (Ribeiro et al., 2009). Hydrolysis was performed with KOH methanolic solution (11 g/L), at 90 ºC, for 10 min. The free fatty acids originally present and those resulting from the alkaline hydrolysis were derivatized to their methyl ester forms with BF3 methanolic solution (10%), at 90 ºC, for 10 min. The methyl esters derivatives were extracted with isooctane and anhydrous sodium sulphate was added to assure the total absence of water. The resulting extracts were evaporated to dryness under a stream of nitrogen and redissolved in isooctane. The assays were performed in triplicate.
The GC-ITMS analysis was performed according to Ribeiro et al. (2009) with some modifications. The oven temperature was set at 40 °C for 1 min, then increasing 5 °C/min to 250 °C and 3 °C/min to 300 °C. 1 µL of the extract was analysed in Full Scan mode. Identification of compounds was achieved by comparison of their retention index and mass spectra with those from pure standards injected under the same conditions, with National Institute of Standards and Technology (NIST) MS 05 spectral database and with NIST Chemistry WebBook. The fatty acids methyl esters (FAME) quantification was achieved from the calibration curve of the respective FAME standards. The assays were performed in triplicate.
In order to obtain the most complete volatile profile, different extractive methods (solid-phase microextraction (SPME) and chloroform:methanol extraction) were used.
Several commercial fibres can be used to extract volatiles. According to bibliography, recommendations of supplier (Supelco, Bellefonte, PA, USA) and to our own knowledge (Guedes de Pinho et al., 2009) the fibre used was coated with divinylbenzene/polydimethylsiloxane (DVB/PDMS), 65 µm.
Headspace solid-phase microextraction (HS-SPME)
Flowering tops powder (0.5 g) and aqueous lyophilized extract (0.2 g) were stirred (200 rpm) at 40 °C, for 10 min. The fibre was then exposed to the headspace for 5 min, without agitation at 40 ºC. Afterwards the fibre was pulled into the needle sheath and the SPME device was removed from the vial and inserted into the injection port of the GC system for thermal desorption, for 1 min. The fibre was then removed and conditioned in another GC injection port for 10 min at 250 °C.
The volatiles analysis was performed as mentioned in Guedes de Pinho et al. (2009), with some modifications. The injector port was heated to 220 °C. Chloroform:methanol extract was redissolved in methanol and injected in split mode, with split ratio of 1/40. The oven temperature was set at 40 ºC (for 1 min), then increased at 2 °C/min to 88 °C. Ionization was maintained off during the first 3 min, to avoid solvent overloading. Covered mass ranged from 33-350 m/z, with a scan rate of 6 scan/s. The injection volume was 1 µL and the analysis was performed in Full Scan mode.
HS-SPME analysis of flowering tops powder and aqueous lyophilized extract was performed in Full Scan mode using the same equipment, under the same oven temperature conditions. The GC injector liner was a SPME specific one and the injection was done in splitless mode. Ionization was kept off for only 1 min.
All compounds were identified by comparing their retention indices and mass spectra with those of authentic reference compounds injected under the same conditions, and with NIST 05 MS Library Database. Peaks' areas were determined by reconstructed FullScan chromatogram using for each compound some specific ions, quantification ions (Table 2). By this way some peaks which were co-eluted in FullScan mode (resolution value less than 1) could be integrated with a value of resolution higher than 1. All samples were analyzed in triplicate.
Aqueous lyophilized extract was used in the screening of antioxidant activity.
Antiradical activity was determined spectrophotometrically in a Multiskan Ascent plate reader (Thermo; electron corporation), by monitoring the disappearance of DPPH at 515 nm, according to a described procedure (Oliveira et al., 2009).
Nitric oxide assay
The nitric oxide scavenging capacity was determined by using Griess reagent, in a Multiskan Ascent plate reader (Thermo; electron corporation), as before (Oliveira et al., 2009).
Superoxide anion assay
Superoxide was generated by the NADH/PMS system and the effect of the extract on radical-induced reduction of NBT was monitored spectrophotometrically in a Multiskan Ascent plate reader (Thermo, electron corporation), as reported in Oliveira et al. (2009).
Hypochlorous acid assay
The inhibition of hypochlorous acid-induced 5-thio-2-nitrobenzoic acid (TNB) oxidation to DTNB was evaluated spectrophotometrically in a Multiskan Ascent plate reader (Thermo, electron corporation), at 412 nm, as previously reported (Vrchovská et al., 2006), with some modifications. The reaction mixtures in the sample wells consisted of 160 µL of redissolved lyophilized extract, 260 µL of TNB and 180 µL of hypochlorous acid solution.
Acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) inhibitory activities
Aqueous lyophilized extract was used in the screening of cholinesterase inhibitory properties. The inhibition of AChE activity was determined spectrophotometrically in a Multiskan Ascent plate reader (Thermo Electron Corporation), based on Ellman's method, as previously reported (Oliveira et al., 2009). BuChE inhibition assay was performed in a similar way, using 25 µL of substrate (15 mM butyrylthiocholine chloride) and 25 µL of BuChE (0.1 U/mL).
Nine bacteria species were used: Staphylococcus aureus (ATCC 20231), Staphylococcus epidermidis (ATCC 20044), Micrococcus luteus (ATCC 20030), Enterococcus faecalis (ATCC 20477), Bacillus cereus (ATCC 31), Proteus mirabilis (ATCC 4479), Escherichia coli (ATCC 30083), Pseudomonas aeruginosa (ATCC 50071) and Salmonella typhimurium (ATCC 43971). All cultures were obtained from the Laboratory of Microbiology, Faculty of Pharmacy, Porto University, Portugal. Stock cultures were maintained on MHA at 4 ºC.
Preparation of inocula
Bacterial inocula were prepared by growing cells in MHB for 24 h, at 37 ºC. Cell suspensions were diluted in sterile MHB to provide initial cell counts of about 106 colony-forming units per mL (CFU/mL).
Minimum inhibitory concentration (MIC)
The minimum inhibitory concentration of C. tinctoria flowering tops extracts was assessed by the twofold serial dilution method, as described by Taveira et al. (2010). The plant extracts were redissolved in methanol (5%, v/v) and diluted in MBH. MIC were determined as the lowest concentration able to inhibit the visible growth of the tested culture.
Results and Discussion
Fatty acids composition
Fatty acids were present in both chloroform:methanol and aqueous lyophilized extracts (Table 1). Comparing both extracts, we can observe that chloroform:methanol is the one with higher diversity and fatty acids content (Table 1 and Figure 1), which is not surprising considering the low polarity of these compounds and, consequently, their higher affinity for the organic solvent. Fifteen fatty acids were identified, being distributed by saturated fatty acids (capric, lauric, myristic, pentadecanoic, palmitic, heptadecanoic, stearic, arachidic, heneicosanoic, behenic, tricosanoic and lignoceric acids), monounsaturated fatty acids (palmitoleic and oleic acids) and a polyunsaturated fatty acid (linolenic acid) (Table 1). As far as we know, this is the first report describing the fatty acid composition of C. tinctoria (Asteraceae) flowering tops.
This vegetal material is essentially constituted by saturated fatty acids (Table 1), unlike what happens with seeds, which were previously reported to mainly contain linoleic acid, a polyunsaturated fatty acid (C18:2) (Coxworth, 1965). Myristic was the major fatty acid, corresponding to ca. 26.3 and 29.6% of total fatty acids content in chloroform:methanol and aqueous lyophilized extracts, respectively (Table 1).
Palmitoleic was the only monounsaturated fatty acid present in the aqueous lyophilized extract and linolenic and oleic acids were found just in the chloroform:methanol extract (Table 1).
Sixteen volatile compounds were identified in C. tinctoria flowering tops, being distributed by distinct chemical classes: 1 aldehyde, 13 monoterpenes and 2 sesquiterpenes, being monoterpenes the compounds present in highest amounts (Table 2 and Figure 2). As far as we know, this is the first study characterizing the volatile composition of this matrix. Comparing the two extracts and the powdered sample, we can observe that the flowering tops powder is characterized by higher diversity and content of volatile compounds and that the chloroform:methanol extract is the poorest one (Table 2). α-Pinene (ca. 45.2% of total compounds area) and trans-carveol (ca. 28.6%) were the volatiles present in higher amounts in C. tinctoria powder and aqueous lyophilized extract, respectively (Table 2). In the chloroform:methanol extract, carvone and cis-carveol were the major compounds, corresponding to ca. 35.3 and 34.8 %, respectively (Table 2). Amongst monoterpenes we can highlight the presence of α-thujone, β-linalool, β-terpineol, borneol and bornyl acetate only in flowering tops powder. On the other hand, limonene was found just in chloroform:methanol extract which may be related with its higher affinity for the organic solvent.
Some of these compounds (α-pinene, limonene, β-caryophyllene, camphene and bornyl acetate) were reported to occur in Coreopsis genus, being already described in the essential oils of Coreopsis barteri and Coreopsis grandiflora leaves (Menut & Lamaty, 1996). The leaves of C. barteri contained mainly monoterpenes, as it happened with our matrix. On the other hand, C. grandiflora leaves were predominantly composed by sesquiterpenes (Menut & Lamaty, 1996).
In the present work, the antioxidant capacity of C. tinctoria aqueous lyophilized extract was screened by DPPH assay, being observed a concentration-dependent effect (IC50 0.041 mg/mL) (Figure 3A).
C. tinctoria also displayed a protective effect against nitric oxide and superoxide radicals, in a concentration-dependent way, with IC50 values of 0.107 and 0.237 mg/mL, respectively (Figures 3B and 3C). The inhibitory activity over these two radical species is of great importance, once it prevents the formation of other deleterious radicals, like peroxynitrite, which causes damage in cardiovascular and neurological systems and is also able to nitrosylate biomolecules, such as proteins (Beckman, 1996, Zubia et al., 2007, Pacher et al., 2007). C. tinctoria capacity for scavenging hypochlorous acid was also evaluated, but no effect was observed at the tested concentrations.
Overall, the results obtained in the three assays revealed C. tinctoria flowering tops' capacity to scavenge free radicals, which can be partially related to the presence of monoterpenes with known antioxidant properties, as α-pinene (Singhara et al., 1998). Nevertheless, the existence in this species of other non-determined compounds, with antioxidant capacity, cannot be ignored.
AChE and BuChE inhibitory activities
One possible strategy for enhancing cholinergic activity in the brain is by the inhibition of AChE and BuChE, responsible for the cleavage of the neurotransmitter acetylcholine (Houghton et al., 2006). According to Houghton et al. (2006), compounds like terpenes have the capacity to inhibit these enzymes. C. tinctoria aqueous lyophilized extract presents a high content of monoterpenes, including α-pinene, which is found at an amount reported to exert activity against AChE and BuChE (10-15%) (Perry et al., 2000; Houghton et al., 2006). Despite this, no effect was observed. The absence of AChE and BuChE inhibitory activities may be due to the existence of interactions between the different compounds present in the tested aqueous extract, namely antagonisms.
The chloroform:methanol and aqueous lyophilized extracts from C. tinctoria flowering tops were screened against Gram-positive (S. aureus, S. epidermidis, M. luteus, E. faecalis and B. cereus) and Gram-negative (P. mirabilis, E. coli, P. aeruginosa and S. typhimurium) bacteria, allowing the determination of some MIC, which are indicative of the antibacterial properties of this species (Table 3). Under the tested concentrations, Gram-positive bacteria were most susceptible to both tested extracts and, in a general way, chloroform:methanol seems to be the most effective one (Table 3). Previous studies indicated that Gram-positive bacteria appear to be more sensitive to the action of natural extracts (Dorman & Deans, 2000; Taveira et al., 2010), which is in agreement with our results. These results can probably be related to the physical differences between the cell walls of Gram-positive and Gram-negative bacteria. The presence of the external membrane with high content of lipopolysaccharide surrounding the cell wall in Gram-negative bacteria is considered to be the major contributor to its ability to resist chemical stress (Nohynek et al., 2006). In a general way, E. faecalis and B. cereus were the most susceptible organisms. E. faecalis, a member of the healthy human intestinal flora, can cause several infections, especially in nosocomial environments (Shankar et al., 2002). B. cereus is a cause of food poisoning, which is frequently associated with the consumption of rice-based dishes. This organism produces an emetic or diarrheal syndrome induced by an emetic toxin and enterotoxin, respectively (Drobniewski, 1993).
C. tinctoria flowering tops' antibacterial activity may be due, at least partially, to the presence of fatty acids, which are well-known for their bactericidal potential (Kabara et al., 1972). Fatty acids and derivatives are more effective against Gram-positive than Gram-negative organisms (Sheu & Freese, 1973). The mechanism of bactericidal action of long-chain fatty acids and derivatives is ascribed to a balance between the hydrophilic and hydrophobic parts of the molecule. Regardless of the polarity of the hydrophilic portion, optimum chain length is between C12 and C16 (Sheu & Freese, 1973). In fact, C. tinctoria aqueous and chloroform:methanol extracts were characterized by high quantities of these kinds of compounds, which correspond to ca. 34 and 48% of their total fatty acids content, respectively. In addition, lauric acid (C12), the second major fatty acid in the chloroform:methanol extract (ca. 13.2% of total fatty acids), is recognized by its high capacity to inhibit Gram-positive bacteria (Kabara et al., 1972). Also the antibacterial action of long-chain unsaturated fatty acids, including oleic and linolenic acids (C18), present in chloroform:methanol extract, is well known (Seidel & Taylor, 2004).
Furthermore, this activity may also be related with the presence of volatile compounds. Among these, we can highlight the presence of carvone in the chloroform:methanol extract, of benzaldehyde, α-pinene and myrtenal in the aqueous lyophilized one and of trans-carveol in both of them, which are known for their antimicrobial properties (Sivropoulou et al., 1995, Dewick, 1997, Baby et al., 2006, Kochi et al., 1980).
The fatty acids and volatile composition of C. tinctoria, Asteraceae, flowering tops, as well as their antioxidant and antibacterial activities were described for the first time, improving the knowledge of the metabolome and biological potential of the species. These results can contribute for an increment on the consumption of this matrix. Furthermore, C. tinctoria can be used in food industry as food preservative, or can be applied in cosmetic and pharmaceutical industries providing protection against several diseases.
APO (SFRH/BD/47620/2008) is indebted to Fundação para a Ciência e a Tecnologia (FCT) for the grant. The authors are grateful to FCT for grant no. PEst-C/EQB/LA0006/2011.
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Received 6 May 2011
Accepted 1 Jun 2011
* Correspondence: Patrícia Valentão, REQUIMTE/Laboratório de Farmacognosia, Departamento de Química, Faculdade de Farmácia, Universidade do Porto. R. Aníbal Cunha, 164, 4050-047 Porto, Portugal. Tel. +351 222078934, Fax: +351 222003977, firstname.lastname@example.org