SciELO - Scientific Electronic Library Online

vol.22 issue6True and common balsams author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Revista Brasileira de Farmacognosia

Print version ISSN 0102-695X

Rev. bras. farmacogn. vol.22 no.6 Curitiba Nov./Dec. 2012  Epub Aug 23, 2012 

Genus Mikania: chemical composition and phytotherapeutical activity



Luciane C. RufattoI; Adriana GowerII; Joséli SchwambachI; Sidnei MouraI,II

IInstituto de Biotecnologia, Universidade de Caxias do Sul, Brazil
IICentro Tecnológico, Universidade de Caxias do Sul, Brazil





The genus Mikania ranks high in the list of best-selling natural products in the world. Its main distribution is in South America, but some species are found in Asia, North America and Africa. It is used for treating fever, rheumatism, colds and respiratory diseases, as well as snake bites and scorpion stings, due to its broad spectrum of action. There are approximately 430 species of this genus and only 12% have been studied, highlighting their chemical and pharmacological diversity. The main chemical groups are: coumarins and derivatives, sesquiterpenes, sesquiterpenes lactones, diterpenes, phytosterols/terpenoids and flavonoids. This review aims to supply useful references for scientists interested in natural products and the search for new compounds, from over the 300 already described for the genus.

Keywords: chemical composition, Mikania, pharmacological activity, review




For centuries, many medicinal plants have been used all over the world as a sort of treatment of various diseases through yet unknown mechanisms. The number of herbal medicines has increased every year, and the phytotherapic market trades billions of dollars annually (Marcus & Grollman, 2002). Moreover, most compounds present in plants may cause serious side effects. Hence, the correct identification and separation of chemical structures of the major components is crucial, making the use of active medicinal plants safe.

The genus Mikania is the largest of its kind in the Eupatorieae (Asteraceae) tribe, with more than 430 species concentrated mainly in the tropical regions (King & Robinson, 1987). Although it is one of the most distinctive and easily recognized genera of the tribe, species delimitation is often difficult due to the very large number of taxa and the existence of highly polymorphic species complexes (King & Robinson, 1987). In Brazil, the genus with 171 species is mainly found in the states of São Paulo, Minas Gerais and Rio de Janeiro (Gasparetto et al., 2010). The species of this genus are characterized by herbaceous, annual or perennial (Pio Correa, 1984), and scandent habit, though there are as well commonly erect and decumbent representatives (Ritter & Miotto, 2005). Some species known as "guaco" have shown a broad spectrum of action and are used to treat fever, rheumatism, colds and respiratory diseases (Silva et al., 1984; Moura et al., 2002; Oliveira et al., 2007; Soares et al., 2007; Freitas et al., 2008). Approximately 12% of Mikania species and their chemical composition have been studied. The most commonly used are Mikania glomerata and Mikania laevigata, generally employed in respiratory disorders treatments (Gasparetto et al., 2010), and because their morphological and anatomic similarities are sold indiscriminately and used without distinction (Ritter & Miotto, 2005; Bolina et al., 2009). However, other species are described in the literature, and are characterized for their chemical components activities. This review aims to identify key species, their chemical components and the main herbal medicine action reported as a guide to future research on the Mikania genus.

Chemical composition

Different classes of compounds were previously isolated from various Mikania parts, which can be associated to this plant's pharmacological activities. The main groups are: coumarins and derivatives, sesquiterpenes, sesquiterpenes lactones, diterpenes, phytosterols/terpenoids and flavonoids. Caffeoylquinic acid derivatives beyond others chemical compounds are found in smaller amount. Diterpenes such as kaurenoic acid and benzoylgrandifloric acid (class of kauranes), have also attracted interest for their pharmacological action. Moreover, detailed screenings revealed the presence of other substances in species of Mikania as alcohols, acids, esters, aldehydes and organic esters (Gasparetto et al., 2010).

Coumarins and derivatives

The most characteristic class of compounds in Mikania genus are the coumarins and derivatives, frequently responsible for pharmacological activity. A wide variety of biological activities is assigned for these compounds, such as antimicrobial, antiviral, anti-inflammatory, antispasmodic, antitumoral, anticoagulant, bronchodilator and antioxidant (Pereira et al., 1992; Hoult & Payá, 1996). The coumarin (1,2-benzopyran) (1), dihydrocoumarin and O-coumaric acid were identified in extracts of M. glomerata (Vidal et al., 2006) and M. laevigata (Oliveira et al., 1984). Herz & Kulanthaivel (1985) discovered in M. congesta aerial parts, growing in the state of Pará-Brazil, some similar compounds such as scopoletin (2), O-geranylscopoletin. In M. shushunensis, collected in Peru, herniarin (7-methoxycoumarin) (3) and 2,6-dimethoxyquinone (Gutierrez & Herz, 1988) has been described.

Sesquiterpenes and terpenes, diterpenes and sesquiterpenes lactones

Sesquiterpenes are abundant in Mikania genus, related to that the most commom are germacrene D, isocomene and γ -humulene. These compounds were reported in around 15% of Mikania species that already had their chemical composition determined, among them M. arrojadoi (Bohlmann et al., 1982b), M. officinalis, M. sessilifolia, M. luetzelburgii and M. belemii (Bohlmann et al., 1981). Chart 1 presents in detail the species studied and the compounds that have been described.

Likewise, terpenes, diterpenes and sesquiterpene lactones are often found, mainly the dilactones type mikanolide and miscandenin derivatives, which have analgesic activity (Ahmed et al., 2001), antibacterial (Facey et al., 2010) and anticancer properties (Prevost et al., 2002). A list of species and the lactones compounds described for each of them is presented in Chart 2.




Some diterpenes are common in Mikania genus like kaurenoic acid (44), the main component of ethanolic extract in M. obtusata (Alves et al., 1995) and M. glomerata (Barbosa et al., 1994), which is characterized by its trypanocidal activity. Also, the kaurenoic acid has other important activities such as antimicrobial, antinociceptive, anti-inflammatory and smooth muscle relaxant (Costa-Lotufo et al., 2002; Wilkens et al., 2002; Cunha et al., 2003; Gasparetto et al., 2012).

In M. laevigata the main representatives are cinnamoylgrandifloric acid (45), isopropiloxigrandifloric acid, isobutiloxi-grandifloric acid and kaurenol (Oliveira et al., 1984; Bighetti et al., 2005; Yatsuda et al., 2005; Santos et al., 2006; Bolina et al., 2009). Furthermore, from M. oblongifolia aerial parts were obtained cinnamoylgrandifloric and others terpenes (Vichnewski et al., 1977).

Cruz & Roque (1992) isolated from M. triangularis stems, found in the state of São Paulo, Brazil, a new diterpene acid, methyl ent-7-α-hidroxypimara-8,15-dien-19-oate and other diterpene acids known as methyl-ent-pimara-9(11),15-dien-19-oate (46), methyl-ent-pimara-8,15-dien-19-oate (47), methyl-8,9α-epoxy-ent-pimara-15-en-19-oate (48), methyl-7β-hydroxy-ent-pimara-8,15-dien-19-oate and methyl-7α-hydroxy-ent-pimara-8,15-dien-19-oate. In addition, the acidic fraction of hexane extract is composed of various pimaradienes acids, which show antibacterial activity (Cruz et al., 1996).

In a study performed by Nunez et al. (2004) on leaves of Mikania sp. nov., found in the state of Bahia, Brazil, several diterpenes were obtained: labda-8(17),12,14-trien-19-oic methyl ester (49), pimara-9(11),15-dien-19-oic methyl ester, labda-8(17),13(16),14-trien-19-oic methyl ester, labda-12α-epoxy-8(17),14-dien-19-oic methyl ester, labda-12β-epoxy-8(17),14-dien-19-oic methyl ester (50), erythroxyla-3,15-dien-19-oic acid (51), labda-12,15-epoxy-8(17),13-dien-19-oic acid (52), and labda-12,13-dihydroxy-8(17),14-dien-19-oic methyl ester.

Phytosterols/terpenoids The most common phytosterols present in approximately 10% of species of Mikania, that has its chemical composition determined, are stigmasterol (53), lupeol (54) and sitosterol. These compounds have been detected in the aerial parts and are found in the species M.micrantha (Herz et al., 1975; Nicollier & Thompson, 1981), M. glomerata (Barbosa et al., 1994), M. cordata (Kiang et al., 1968; Aguinaldo et al., 1995), M. cordifolia (Oliveira et al., 2006), M. minima (Cuenca & Catalán, 1990), M. hoehnei (Chaves & Oliveira, 2003), M. grazielae (Bohlmann et al., 1982b), M. pseudohoffmanniana (Souza et al., 2006), M. stipulacea (Nascimento & Oliveira, 2001; Nascimento et al., 2004) and M. pohlii (Bohlmann et al., 1982a).


Click to enlarge


The presence of other common phytosterols as campesterol and taraxasterol has been reported in species like M. cordifolia (Oliveira et al., 2006), M. laevigata (Ferreira & Oliveira, 2010), M. hoehnei (Chaves & Oliveira, 2003) and M. parodii (Gregorio et al., 2008).

The terpenoids amyrin and friedelin (54), abundant in Mikania genus, were reported in M. micrantha, M. cordata, M. cordifolia (Oliveira et al., 2006), M. minima and M. lasiandrae (Soares et al., 2007), among others species. Other terpenoids less common but equally important for their antioxidant activity, such as squalene, were found in several species like M. grazielae (Bohlmann et al., 1982b), M. sessilifolia (Bohlmann et al.,1981), M. luetzelburgii (Bohlmann et al., 1981) and M. officinalis (Bohlmann et al., 1981).



Among the exotic representatives of terpenoids present in Mikania genus were reported τ-muurolol in M. hookeriana (Reis et al., 2003), stigmasta-4,22-dien-3-one in M. microptera, olean-9(11),13-dien-3-one in M. rimachii (Díaz et al., 1992) and 19,20-dihydroxy-16-oxo-geranyl from M. luetzelburgii (Bohlmann et al., 1981).


Flavonoids are popular due to their antioxidant activity and are widely present in Mikania genus supporting its pharmacological activity. In M. laevigata flavonoids glycosides as patuletin 3-O-β-D-glucopyranoside (56), kaempferol 3-O-β-D-glucopyranoside (57), quercetin 3-O-β-D-glucopyranoside (58) and 3,3',5-trihydroxy-4',6,7-trimethoxyflavone are the representatives compounds (Ferreira & Oliveira, 2010). In M. cordata, flavonoids weredescribed as patuletine-3-O-β-D-6"-(p-coumaroyl), glucoside(6-methoxyquercetin-3-O-β-D-6"-(p-coumaroyl)glucoside), mikanin-3-O-sulfate (salt as Ca+2) (59), eupalitin-3-O-sulphate (as salt K+) (60), eupalitin-3-O-β-D-glucoside (61), 6-methoxykaempherol-3-O-β-D-glucoside (62), nepetin (63) and kaempherol-3-O-α-L-rhamnoside (64) (Aguinaldo et al., 2003). For the same species, it was reported the isolation of a flavone, mikanin-(3,5-dihydroxy-4',6,7-trimethoxyflavone) with epifriedelinol from roots and fumaric acid from leaves and stems (Kiang et al., 1965).

In M. cordifolia a quercetin derivative was identified as quercetin-3-O-glucoside. In M. micrantha was isolated and identified eupalitin, eupafolin, luteolin (65) (Wei et al., 2004), mikanin, alpinetin, mikanin-3-O-sulfate (59) (Herz et al., 1975; Nicollier & Thompson, 1981; Boeker et al., 1987; Cuenca et al., 1988; Jiang et al., 2001). In the same specie was identified 3,4',5,7-tetrahydroxy-6-methoxyflavone3-O-β-D-glucopyranoside by Huang et al. (2009). Kaempferol-3-O-glucoside (57) and quercetin-3-O-glucoside (58) were identified from M. parodii (Gregorio et al., 2008). Naringenin (66) was identified from M. grazielae (Bohlmann et al., 1982b) and a specific flavone derivative called batatifolin was found in M. batatifolia (Herz & Santhanam, 1969).

Caffeoylquinic acid and derivatives

The chemical compound 5-caffeoylquinic acid is a caffeic acid ester, also known as a chlorogenic acid, commonly found in a wide number of plants, e.g. coffee. It is produced in plants via an ester bond between the carboxyl group of caffeic acid and the 5-hydroxyl group of quinic acid (Clifford et al., 2006). The chlorogenic acid and caffeic acid were reported as dampening the risk of chronic diseases such as inflammation, cardiovascular diseases and cancer (Boyer & Liu, 2004; Bonita et al., 2007).

In M. micrantha was reported the presence of 3,5-di-O-caffeoylquinic acid n-butyl ester and 3,4-di-O-caffeoylquinic acid n-butyl ester (Wei et al., 2004). The same 3,5-di-O-caffeoylquinic acid (67) was reported in M. cordifolia, beyond others derivatives like 5-O-caffeoylquinic acid, 3,4-di-O-caffeoylquinic acid (68), 4,5-di-O-caffeoylquinic acid and 3-O-feruloyl,5-O-caffeoylquinic acid (Gregorio et al., 2008). In M. lasiandrae was also reported the presence of caffeoylquinic acid (Soares et al.,2007). In M. hirsutissima was reported the presence of 1,5-dicaffeoyl-quinic acid (Oliveira, 1972; Ohkoshi et al.,2004).



Pharmacological activities

A given plant provides the investigator with a complex library of unique bioactive constituents and can be an advantageous strategy to prospect new pharmacological compounds. The task of the natural products researcher is to select those compounds of pharmacological interest through bioassay-guided fractionation of the "natural combinatorial libraries" produced by extraction, and then to collaborate in the optimization and development of the lead natural product structure.

The Mikania species have multiple pharmacological actions. In general, activity in respiratory tract, anti-inflammatory, anti-allergic, analgesic, antioxidant even in system nervous central. In this section, our aim is to highlight the pharmacological experiments and studies reported with species of the genus Mikania.

Activity in the respiratory tract

Medicinal plants play an important role in maintaining public health, mainly due to their low cost and availability. Some plants acting in the respiratory system, such as Mikania genus, have confirmed their effectiveness. For example, M. glomerata, one of the most important and commonly used species Mikania genus, has been popularly used in the treatment of asthma, bronchitis and coughing (Teske & Trentini, 1997; Silva et al., 2006; Agra et al., 2008). Other species, known as "guaco" are also used to treat respiratory problems as M. cordifolia (Oliveira et al., 2006, Caribe & Campos, 1991), M. laevigata (Bolina et al., 2009) and M. cordata (Ali et al., 2011)

Other studies have shown the use of M. glomerata and M. laevigata as expectorant, in the treatment of influenza and respiratory diseases (Lorenzi & Matos, 2002; Gasparetto et al., 2010). The coumarin seemed to be partially responsible for the bronchodilator activity of the plant through the relaxation of smooth muscle. In addition, Moura et al. (2002) verified that the aqueous and hydro-alcoholic extracts (HAE) obtained from M. glomerata induced a significant inhibition on the histamine contractions on the isolated guinea-pig trachea. HAE induced a concentration-dependent relaxation on guinea-pig trachea precontracted with histamine (IC50 0.34 mg mL-1), acetylcholine (IC50 0.72 mg mL-1) or K+ (IC50 1.41 mg mL-1) and on isolated human bronchi precontracted with K+ (IC50 0.34 (0.260.42) mg mL-1). In studies evaluating the HAE of M. laevigata for the treatment of respiratory diseases it was found that the extract produced a dose dependent relaxation in denuded and intact rat epithelium tracheal precontracted with acetylcholine with an effective concentration (EC50) of 1406.7 µg/ml and 1378.3 µg/ml respectively, and a maximum effect (Emax) of 94.7 and 95.7% respectively (Graça et al., 2007; reviewed in Gasparetto et al., 2012). These data supports the indication that M. glomerata and M. laevigata can be used to treat bronchoconstrictive respiratory diseases.

Activity in the digestive system

Many plants and their extracts are commonly used for acting against several disorders of the digestive system. Among them are some species of the genus Mikania as M. glomerata, M. laevigata and M. cordata.

In a study performed by Salgado et al. (2005) with M. glomerata, the aqueous extract of leaves (1000 mg/mL) showed a decrease in the propulsive movements of the intestinal contents in mice. Oral administration produced an inhibition of gastrointestinal transit as effective as that produced by loperamide, a reference antidiarrheal drug. These findings suggested that the aqueous extract of the leaves of M. glomerata might elicit an antidiarrheal effect by inhibiting intestinal motility.

Moreover, the decoction of the leaves of M. cordata also shows effects in the digestive system. It is used in dyspepsia, dysentery and gastric ulcer (Ghani, 1998). The methanolic fraction of root extract showed antiulcer effects in male Sprague-Dawley rats in a dose dependently manner inhibiting gastric ulcers induced by water immersion stress-induced, ethanol, aspirin and phenylbutazone. The ED50 values of the extract in the above four ulcer models were found to be 95.1, 109.7, 125.5 and 136.2 mg/kg, respectively (Bishayee & Chatterjee, 1994b). In the study realized by Paul et al. (2000) the alkaloid fraction obtained from an ethanolic extract of leaves of Mikania cordata (dose 50 mg/kg) exhibited antiulcer activity (in vivo) in gastric erosions induced by diclofenac sodium in rats Long Evans. Mosaddik & Alam (2000) carried out a similar study to evaluate the role of alkaloids fraction in gastric erosions induced by diclofenac sodium in rats Long Evans and found that in the alkaloidal-administered group (50 mg/kg) the ulcer index of the stomach (0.268±0.0346) and of the duodenum (0.050±0.0129) were significantly lower than the diclofenac-only administered group (0,691±0,0184 and 0.093±0.0346, respectively). Thus, bioactive principles of M. cordata have proved anti-ulcerogenic effects.

The crude hydroalcoholic 70% extract of M. laevigata presents antiulcerogenic activity when applied in male Wistar rats decreasing the ulcerative index produced by indomethacin, ethanol, stress and reserpine by 85, 93, 82 and 50%, respectively (Bighetti et al., 2005). In this way, different species of "guaco" show activity in the digestive system.

Effect on nervous system

Mikania extracts possesses some neuropharmacological properties confirmed. The studies with methanolic fraction of M. cordata root extract on experimental animals caused alterations in the general behavior pattern (e.g. reduction in spontaneous motility, analgesia, and suppression of aggressive behaviour), suppression of conditioned avoidance response and showed antagonism to amphetamine toxicity. The observations suggest that the root of M. cordata possesses a potent central nervous system-depressant action (Bhattacharya, et al. 1988).

The hydroacoholic extract of aerial parts from M. scandens presents neuropharmacological properties in Swiss albino mice. The results of the present study revealed significant and dose-dependent (250 and 500 g/kg body weight) central antinociceptive, locomotor depressant, muscle relaxant, and sedative potentiating effects of the extract, demonstrating its depressant action on the central nervous system (Dey et al., 2011).

Anti-inflammatory, anti-allergic and analgesic activity

The inflammatory response is associated with a range of diseases and it is difficult to establish an effective therapy to control the inflammatory processes. So, there is a clear and obvious need to search for new medicinal compounds, especially those derived from plants. Studies with extracts, oils and compounds of several species have demonstrated their important activity. Oliveira et al. (1985) discovered the anti-inflammatory action of the fluid extract of M. glomerata through the antiedema activity test in the rat paw induced by carrageenan and quantified by plethysmography. These inhibitions were slightly smaller than that produced by the control with phenylbutazone.

In addition, Leite et al. (1993) compared the effects of hydroalcoholic extract of M. glomerata and solution of coumarin (1,2-benzopyran), undergoing tests in vivo (paw edema). The results showed anti-inflammatory effect for the extract and also the solution. The different intensity on pharmacological effects indicates that coumarin has contributed to the pharmacological effect with other chemicals in the extract in a synergic action. Ruppelt et al. (1991) studied the "guaco" tea (M. glomerata) as analgesic and anti-inflammatory evaluating the number of contortions in mice and diffusion of Evans blue dye in the peritoneum. The mice group that ingested the tea showed inhibition of 63.1% in contortions and a reduction of 48.92% in diffusion of the dye in comparison with the control group. In this way the plant infusion demonstrated analgesic and anti-inflammatory activity in lesser degree in comparison with the analgesic control.

The compound scandenolide, a sesquiterpene lactone present in M. cordata, exhibited anti-inflammatory activity. It also inhibited the production of leukotriene B4 and 5-HETE with IC50 of 15 and 30 µM concentration, respectively (Ysrael & Croft, 1990). The anti-inflammatory activity of M. cordifolia was attributed to the presence of dicaffeoylquinic acids and was evaluated trough activity on monocyte migration and superoxide anion production (Peluso et al., 1995).

Other species, M. laevigata and M. involucrata demonstrated a potential anti-inflammatory activity in inhibiting oedema and pleurisy. In the induced rat paw oedema test, the animals treated with leaf decoctions of M. laevigata (200 mg/kg) and M. involucrata (50 mg/kg) presented an edema inhibition of 81.56 and 81.67%, respectively, 3 h after the administration of the phlogistic agent. In the pleurisy assay, M. laevigata (400 mg/kg) and M. involucrata (200 mg/kg) leaf decoctions inhibited leukocyte migration to the pleural exudate by 28.26 and 54.35%, respectively (Suyenaga et al., 2002).

The hydroalcoholic extract of M. glomerata and M. laevigata also affected the inflammatory and oxidative stress caused by a single coal dust intratracheal instillation in rat. Histopathological analyses revealed that animals pretreated subcutaneously with the hydroalcoholic extract (100 mg/kg) had a reduction in lung inflammation, with an additional decrease in protein thiol levels, suggesting that guaco has an important protective effect on the oxidation of thiol groups (Freitas et al., 2008).

The analgesic activity of hydromethanol extract of leaves from M. scandens was determined for its central and peripheral pharmacological actions using hotplate and tail immersion method and acetic acid-induced writhing test in mice respectively. The extract (250 and 500 mg/kg), produced a significant increase in pain threshold in hotplate and tail immersion methods in a dose dependent manner. In acetic acid-induced writhing test, the extract (500 mg/kg) produced a maximum of 53.73% inhibition of writhing reaction compared to the reference drug Diclofenac-Na (76%). Thus, the results suggested that the extract has a strong analgesic effect (Hasan et al., 2009). In addition, the crude extract of M. cordata (1 and 3 g/kg) and a sesquiterpene lactone deoxymikanolide (10 mg/kg) significantly inhibited acetic-acid induced writhing in mice (Ahmaed et al., 2001).

Another important activity observed in some species is the anti-allergic activity. Fierro et al. (1999) observed this activity in their study with M. glomerata. A fraction (MG1) obtained from the ethanolic extract used as an anti-allergic and anti-inflammatory agent was evaluated for these properties on ovalbumin-induced allergic pleurisy and in models of local inflammation induced by biogenic amines, carrageenan and PAF. Plasma exudation as well as neutrophil and eosinophil infiltration evoked by the intrapleural injection of the antigen were significantly reduced by the fraction. Likewise, PAF-induced pleural neutrophil migration was inhibited by the treatment with MG1. The results suggest that MG1 is effective in inhibiting immunologic inflammation. In conclusion, many species of Mikania are involved in anti-inflamatory, analgesic and anti-allergic responses.

Antimicrobial, antivirucidal and antiparasitic activity

The antimicrobial and antiparasitic properties of compounds present in plants as products of secondary metabolism have been known empirically for centuries, but only recently they have been scientifically confirmed. Extracts and essential oils from plants proved their efficacy in controlling the growth of a wide variety of microorganisms, including bacteria, fungi, parasites and others.

Staphylococcus aureus PI57 is a bacterium that resists to all antibiotics used in medical practice with the exception of vancomycin. In a study carried out with hexanic extract of M. glomerata, it was observed the inhibition growth of a multiresistant strain of Staphylococcus aureus PI57, verified by antibiogram and bioautography (Amaral et al., 2003). Other studies have shown antibacterial activity of M. glomerata extract, supporting its action against S. aureus (Pessini et al., 2003; Duarte et al., 2004).

In a study performed by Yatsuda et al. (2005), the hexane fraction of extracts from M. laevigata was the most effective in inhibiting the growth of mutans streptococci (MIC values between 12.5 µg/mL and 400 µg/mL, and MBC values between 25 µg/mL and 400 µg/mL). In addition, sub-MIC levels of the crude extracts and their hexane fractions inhibited the adherence of the microorganisms to a glass surface.

In addition, the acidic fraction of hexane extract from M. triangularis, composed of various pimaradienes acids, showed antibacterial activity (Cruz et al., 1996).

But et al. (2009) carried out a study with M. micranthaand the compound 1,10-epoxy-4-germacrene-12,8,15,6-diolide showed activity against respiratory syncytial virus (IC50 37.4 µM) and parainfluenza virus type 3 (IC50 37.4 µM). Additionally potassium mikanin 3-sulfate showed inhibitory activity against parainfluenza virus type 3 (IC50 19.7 µM).

The biological activities described for M. cordifolia include antitrichomonal and antitrypanosomal (Arias et al., 1995; Serrano et al., 2000). The diterpene ent-kaur-16-en-19-oic acid (kaurenoic acid), the main component in ethanolic extract of M. obtusata, has trypanocidal activity determinated byAlves et al. (1995). This compound showed IC50 of 0.5 mg/mL (1.66 mM) against trypomastigote forms of Trypanosoma cruzi, the causative agent of Chagas disease. Furthermore, the ethanolic extract reduced significantly the number of parasites in the blood. The compound also showed antibacterial and antifungal activities (Mathur et al., 1975; Oguntimein, 1987). Other species that showed in vitro trypanocidal properties were M. hoehnei and M. stipulaceae. The diterpene ent-9α-hydroxy-15β-E-cinnamoyloxy-16-kauren-19-oic acid obtained from M. stipulacea diterpene was active towards T. cruzi tripomastigotes, reducing their number by 61.7, 62.8 and 69.4% at 100, 250 and 500 µg/mL and the sesquiterpene lactone 8β-hydroxyzaluzanin isolated from M. hoehnei killed 56.6 and 81.0% of the parasites at the concentration of 250 and 500 µg/ml (Nascimento et al., 2004).

Antiophidic activity

Although serotherapy was discovered one hundred years ago, many rural communities do not have access to antivenoms. In this way, they alternatively use plants with antiophidic activity known in popular culture, such as some species of the genus Mikania.

The antiophidic effect of coumarin present in M. glomerata was tested against the venom of Bothrops jararaca snake and the animal survival rate was evaluated resulting in 40% survival in animals that received treatment comparing to 0% in the control group (Pereira et al., 1994). In the same way, Maiorano (2005) observed the antiophidic activity of M. glomerata root extracts that reduced the hemorrhage zone stimulated by the intradermal injection of Bothrops venom by 80% in rats. This result suggests that there is an interaction between the components of guaco and metalloproteases involving the catalytic sites of these enzymes or essential metal ions, thereby inhibiting their hemorrhagic activities (reviewed in Gasparetto et al., 2012). The aqueous extract of M. cordifolia is also used by ancient rainforest inhabitants to treat snake bites (Caribe & Campos, 1991).

The activity of M. guaco in oral administration was confirmed by Gutierrez (1993). The extract components were effective in mammals to inhibit the letal effects of poisonous animals, such as nauyaque snake and rattlesnake, scorpions, spiders and bees. The author has indicated its use for treating snake bites, scorpion stinging, bee sting and similar.

Antimutagenic and cytotoxic activity

The natural products provide very important chemical libraries that have led to new antimutagenic drugs (Cragg et al. 2009). The antimutagenic activity of M. laevigata was reported by Fernandes & Vargas (2003). They observed an inhibition of the mutagenic effect induced by 2-amino fluorene and sodium azide using the plant extract, and a synergistic effect in the presence of 4-nitroquinoline-1-oxide in Salmonella/microsome assay.

Melampolides isolated from M. minima were identified as (6S,7R,8S)-8,15-diacetoxy-14-hydroxymelampa-1(10),4,11(13)-trien-12,6-olide and (6S,7R,8S)-8,15-diacetoxy-14-oxomelampa-1(10),4,11-(13)-trien-12,6-olide which present cytotoxic activity. This activity was tested against three tumor cell lines, and IC50 values were observed in the order of 10-6 M. The former compound showed an activity level similar to that of salonitenolide diacetate and the later showed a higher activity (Barrero et al., 2000). Differently, the mikanolide, dihydromikanolide and others sesquiterpenes lactones derivatives from Mikania genus were indicated as DNA polymerase inhibitors by Teng et al. (2001).

The chemopreventive role of M. cordata, was evaluated for its effects on phase 1 and 2 of the hepatic drug detoxifying enzyme system in rats (Bishayee & Chatterjee, 1994a). In oral doses of 50, 100, or 150 mg/kg of extract for 4, 8 or 12 weeks results in dose-dependent effects on a marked induction of uridine diphosphoglucuronyl transferase activities of liver microsomes and others effects. The study indicated that the carcinogens would be reduced by specific enhancement of drug-detoxifying enzymes in the liver of rats treated with the plant extract.

The Chart 3 summarizes other pharmacological applications recently reported in literature.

Other potential uses

Allelopathic activity

Allelopathy is defined as any indirect or direct, beneficial or damaging effect, from a plant to other, resulted from the production of chemical products which are released into the environment. The same chemical compounds responsible for the allelopathic activity can be modulated in some pharmacological activity. It has also attracted great interest due to their potential applications in agriculture and therefore has been studied in several plants. The ethanolic extracts of M. laevigata traditionally cultivated in the soil or in a hydroponic system were tested for allelopathic activity. Allelopathic activity was evaluated by the inhibition of germination assay using lettuce seeds. An allelopathic effect was observed for both extracts, although a more expressive activity of traditional "guaco" was verified, since the inhibition of seeds germination was 100%, even in the lower concentration (Baratto et al., 2008).

Other species of this genus, the M. micrantha, also showed allelopathic activity. The aqueous, essential oil and terpenoid fractions exhibit the inhibitory activity against the germination and the growth of several plant species (Nicollier & Thompson, 1981; Ismail & Chong, 2002). Some allelopathic phenolic acids have been detected from the aqueous fraction of M. micrantha (Ismail & Chong, 2002). Other study has indicated that chloroform and ethyl acetate extracts have significant reduction in growth of the seedling. Sesquiterpene lactones and flavone compounds isolated from the chloroform and ethyl acetate also show inhibitory activity (Huang et al., 2009).

Phytotoxic activity

The phytotoxic activity of sesquiterpene lactones germacrane type from M. micrantha was reported in some studies due the presence of deoxymikanolide, dihydromikanolide and micramikanolide (Herz et al., 1975; Huang et al., 2004). According to Huang et al. (2009), these compounds and 3,4',5,7-tetrahydroxy-6-methoxyflavone-3-O-β-D-glucopyranoside showed strong phytotoxicity against B. parachinensis, being the deoxymikanolide the most active.

Summary and future perspectives

Out of 430 species identified from genus Mikania, 55 of them provide over 300 different chemical compounds, among terpenes and derivatives, some alkaloids, saponins, sterols and flavonoids. From its extensive use as herbal medicine, it was identified several of these compounds as being of highly pharmacological interest due to its actions. Besides the activities already identified other actions were not tested yet, once new studies are carried out it may indicate other interesting features of the molecules already extracted from this genus. The high variability in Mikania composition among different species and batches may contribute to equally high variability in activity. In the future, widespread interest in Mikania genus seems certain to ensure continued research with this herb. Moreover, interdisciplinary research and the development of modern combinatorial techniques make possible the discovery of novel agents from these species.



The authors thank the FAPERGS, CAPES and CNPQ for financial support.



Agra MF, Silva KN, Basílio IJLD, França PF, Barbosa-Filho JM 2008. Survey of medicinal plants used in the region Northeast of Brazil. Rev Bras Farmacogn 18:472-508.         [ Links ]

Aguinaldo AM, Abe F, Yamauchi T, Padolina WG 1995. Germacranolides of Mikania cordata. Phytochemistry 38:1441-1443.         [ Links ]

Aguinaldo AM, Padolina WG, Abe F, Yamauchi T 2003. Flavonoids from Mikania cordata. Biochem Syst Ecol 31:665-668.         [ Links ]

Ahmed M, Rahman MT, Alimuzzaman M, Shilpi JA 2001. Analgesic sesquiterpene dilactone from Mikania cordata. Fitoterapia 72:919-921.         [ Links ]

Ali S, Islam S, Rahman M, Islam R, Sayeed MA, Islam R 2011. Antibacterial and cytotoxic activity of ethanol extract of Mikania cordata (Burm.F.) B.L. Robinson leaves. J Basic Clin Pharm 2:103-107.         [ Links ]

Alves TMA, Chaves PPG, Santos LMST, Nagem TJ, Murta SMF, Ceravolo IP, Romanha AJ, Zani CL 1995. A diterpene from Mikania obtusata active on Trypanosoma cruzi. Planta Med 61:85-87.         [ Links ]

Amaral RR, Arcenio-Neto F, Carvalho ES, Teixeira LA, Araújo GL, Sharapin N, Testa B, Gnerre C, Rocha L 2003. Avaliação da atividade IMAO e antibacteriana de extratos de Mikania glomerata Sprengel. Rev Bras Farmacogn 13:24-27.         [ Links ]

Arias AR, Ferro E, Inchausti A, Ascurra M, Acosta N, Rodriguez E, Fournet A 1995. Mutagenicity, insecticidal and trypanocidal activity of some Paraguayan Asteraceae. J Ethnopharmacol 45:35-41.         [ Links ]

Asakawa Y, Takemoto T 1979. Sesquiterpene lactones of Conocephalum conicum. Phytochemistry 18:285-288.         [ Links ]

Baratto L, Lang KL, Vanz DC, Reginatto FH, Oliveira JB, Falkenberg M 2008. Investigação das atividades alelopática e antimicrobiana de Mikania laevigata (Asteraceae) obtida de cultivos hidropônico e tradicional. Rev Bras Farmacogn 18:577-582.         [ Links ]

Barbosa AD, Ferreira RCV, Valente PHM 1994. Atividade antimicrobiana de extratos fluidos de plantas medicinais brasileiras. Lecta 12:153-163.         [ Links ]

Bardón A, Cardona L, Catalán CAN, Pedro JR 1996. 15-Norguaianolides and germacranolides from Mikania mendocina. Phytochemistry 41:845-849.         [ Links ]

Barrero AF, Oltra JE, Rodriguez-Garcia I, Barragán A, Álvarez M 2000. Preparation, stereochemistry and cytotoxic activity of the melampolides from Mikania minima. J Nat Prod 63:305-307.         [ Links ]

Baruah NC, Sarma JC, Barua NC, Sarma S, Sharma RP 1994. Germination and growth inhibitory sesquiterpene lactones and a flavone from Tithonia diversifolia. Phytochemistry 36:29-36.         [ Links ]

Bhattacharya S, Pal S, Chaudhuri AKN 1988. Neuropharmacological studies on Mikania cordata root extract. Planta Med 54:483-487        [ Links ]

Bighetti AE, Antônio MA, Kohn LK, Rehder VLG, Foglio MA, Possenti A 2005. Antiulcerogenic activity of a crude hydroalcoholic extract and coumarin isolated from Mikania laevigata Schultz Bip. Phytomedicine 12:72-77.         [ Links ]

Bishayee A, Chatterjee M 1994a. Anticarcinogenic biological response of Mikania cordata:reflections in hepatic biotransformation systems. Cancer Lett 81:193-200.         [ Links ]

Bishayee A, Chatterjee M 1994b. Protective effects of Mikania cordata root extract against physical and chemical factors-induced gastric erosions in experimental animals. Planta Med 60:110-113.         [ Links ]

Boeker R, Jakupovic J, Bohlmann F, Schmeda-Hirschmann G 1987. Germacra-1,10Z,4-E-dien-12, 8-α-olides from Mikania micrantha. Planta Med 53:105-106.         [ Links ]

Bohlmann F, Adler A, Jakupovic J, King RM, Robinson H 1982a. A dimeric germacranolide and other sesquiterpene lactones from Mikania species. Phytochemitry 21:1349-1355.         [ Links ]

Bohlmann F, Adler A, King RM, Robinson H 1982b. Entlabdanes from Mikania alvimii. Phytochemistry 21:173-176.         [ Links ]

Bohlmann F, Adler A, Schuster A, Gupta RK, King RM, Robinson H 1981. Diterpenes from Mikania species. Phytochemistry 20:1899-1902.         [ Links ]

Bohlmann F, Tsankova E, King RM, Robinson H 1984. Five mikanolide derivatives from Mikania cynanchifolia and their biogenetic relationships. Phytochemitry 23:1099-1101.         [ Links ]

Bolina RC, Garcia EF, Duarte MGR 2009. Estudo comparativo da composição química das espécies vegetais Mikania glomerata Sprengel e Mikania laevigata Schultz Bip. ex Baker. Rev Bras Farmacogn 19:294-298.         [ Links ]

Bonita JS, Mandarano M, Shuta D, Vinson J 2007. Coffee and cardiovascular disease: in vitro, cellular, animal, and human studies. Pharmacol Res 55:187-198.         [ Links ]

Boyer J, Liu RH 2004. Apple phytochemicals and their health benefits. Nutr J 3:5.         [ Links ]

But PP-H, He Z-D, Ma S-C, Chan Y-M, Shaw P-C, Ye W-C, Jiang R-W 2009. Antiviral constituents against respiratory viruses from Mikania micrantha. J Nat Prod 72:925-928.         [ Links ]

Caribe J, Campos JM 1991. Plantas que ajudam o homem. Ed. Cultrix, São Paulo. 319 p.         [ Links ]

Castro V, Jakupovic J, Bohlmann F 1989. Germacranolides from Mikania species. Phytochemistry 28:527-530.         [ Links ]

Catalán CAN, Cuenca MDR, Hernández LR, Joseph-Nathan P 2003. Cis, cis-germacranolides and melampolides from Mikania thapsoides. J Nat Prod 66:949-953.         [ Links ]

Chaves JS, Oliveira, DCR 2003. Sesquiterpene Lactones and other Chemical Constituents of Mikania hoehnei R. J Brazil Chem Soc 14:734-737.         [ Links ]

Chowdhury JU, Nandi NC, Yusuf M 2007. Aromatic plants of Bangladesh:constituents of the leaf and flowers oils of Mikania cordata (Burm.f.) Rob. Indian Perfumer 51:56-59.         [ Links ]

Clifford MN, Marks S, Knight S, Kuhnert N 2006. Characterization by LC-MS(n) of four new classes of p-coumaric acid-containing diacylchlorogenic acids in green coffee beans. J Agr Food Chem 54:40954101.         [ Links ]

Costa-Lotufo LV, Cunha GMA, Farias PAM, Viana GSB, Cunha KMA, Pessoa C, Morais MO, Silveira ER, Gramosa NV, Rao VSN 2002. The cytotoxic and embryotoxic effects of kaurenoic acid, a diterpene isolated from Copaifera langsdorffii oleo-resin. Toxicon 40:1231-1234.         [ Links ]

Cragg GM, Grothaus PG, Newman DJ 2009. Impact of natural products on developing new anti-cancer agents. Chem Rev 109:3012-3043.         [ Links ]

Cruz FG, Roque NF 1992. Diterpene acids from Mikania triangularis. Phytochemistry 31:2793-2796.         [ Links ]

Cruz FG, Roque NF, Giesbrecht AM, Davino SC 1996. Antibiotic activity of diterpenes from Mikania triangularis. Fitoterapia 67:189-190.         [ Links ]

Cuenca MDR, Bardon A, Catalan CAN 1988. Sesquiterpene lactones from Mikania micrantha. J Nat Prod 51:625-626.         [ Links ]

Cuenca MDR, Borkosky S, Catalán CAN, Díaz JG, Herz W 1992. A cadinanolide and other sesquiterpene lactones from Mikania haenkeana. Phytochemistry 31:3521-3525.         [ Links ]

Cuenca MDR, Borkosky S, Catalán CAN, Goedken VL, Díaz JG, Herz W 1993. Sesquiterpene lactones of Mikania minima. Phytochemistry 32:1509-1513.         [ Links ]

Cuenca MDR, Catalán CAN 1990. 14,15-dihydroxygermacranolides and other constituents of Mikania minima. J Nat Prod 53:686-691.         [ Links ]

Cunha KMA, Paiva LAF, Santos FA, Gramosa NV, Silveira ER, Rao VSN 2003. Smooth muscle relaxant effect of kaurenoic acid, a diterpene from Copaífera langsdorffii on rat uterus in vivo. Phytotherapy Res 17:320-324.         [ Links ]

Dey P, Chandra S, Chatterjee P, Bhattacharya S 2011. Neuropharmacological properties of Mikania scandes (L.) Willd. (Asteraceae). J Advanced Pharm Technol Res 2:255-259.         [ Links ]

Díaz JG, Goedken VL, Herz W 1992. Sesquiterpene lactones and other constituents of Mikania rimachii and Mikania microptera. Phytochemistry 31:597-608.         [ Links ]

Duarte MCT, Figueira GM, Pereira B, Magalhães PM, Delarmelina C 2004. Atividade antimicrobiana de extratos hidroalcoólicos de espécies da coleção de plantas medicinais CPQBA/UNICAMP. Rev Bras Farmacogn 14:6-8.         [ Links ]

Fabbri H, Oliveira DCR, Vichnewski W, Herz W 1997. Diterpenes of Mikania lindbergii Baker. Biochem Syst Ecol 25:563-564.         [ Links ]

Facey PC, Peart PC, Porter RBR 2010. The antibacterial activities of mikanolide and its derivatives. W Indian Med J 59:249-252.         [ Links ]

Facey PC, Pascoe KO, Porter RB, Jones ADJ 1999. Investigation of plants used in Jamaican folk medicine for anti-bacterial activity. J Pharm. Pharmacol 51:1455-1460.         [ Links ]

Fernandes JBF, Vargas VMF 2003. Mutagenic and antimutagenic potential of the medicinal plants M. laevigata and C. xanthocarpa. Phytotherapy Res 17:269-273.         [ Links ]

Ferreira FP, Oliveira DCR 2010. New constituents from Mikania laevigata Shultz Bip.ex Baker. Tetrahedron Lett 51:6856-6859.         [ Links ]

Fierro IM, Silva AC, Lopes CD, Moura RS, Barja-Fidalgo C 1999. Studies on the anti-allergic activity of Mikania glomerata. J Ethnopharmacol 66:19-24.         [ Links ]

Freitas TP, Silveira PC, Rocha LG, Rezin GT, Rocha J, Citadini-Zanette V, Romao PT, Dal-Pizzol F, Pinho RA, Andrade VM, Streck EL 2008. Effects of Mikania glomerata Spreng. and Mikania laevigata Schultz Bip. ex Baker (Asteraceae) extracts on pulmonary inflammation and oxidative stress caused by acute coal dust exposure. J Med Food 11:761-766.         [ Links ]

Gasparetto JC, Campos FR, Budel JM, Pontarolo R 2010. Mikania glomerata Spreng. e M. laevigata Sch. Bip. ex Baker, Asteraceae:estudos agronômicos, genéticos, morfoanatômicos, químicos, farmacológicos, toxicológicos e uso nos programas de fitoterapia do Brasil. Rev Bras Farmacogn 20:627-640.         [ Links ]

Gasparetto JC, Pontarolo R, Francisco TMG, Campos 2012. Mikania glomerata and M. laevigata: Clinical and Toxicological Advances, Toxicity and Drug Testing, Intech.         [ Links ]

Ghani A 1998. Medicinal plants of Bangladesh. Asiatic Society of Bangladesh, 233.         [ Links ]

Graça C, Baggio CH, Freitas CS, Rattmann YD, Souza LM, Cipriani TR, Sassaki GL, Rieck L, Pontarolo R, Silva-Santos JE, Marques MCA 2007. In vivo assessment of safety and mechanisms underlying in vitro relaxation induced by Mikania laevigata Schultz Bip. Ex Baker in the rat trachea. J Ethnopharmacol 112:430-439.         [ Links ]

Gregorio LE, Moraes SL, Pott A, Oliveira DCR 2008. Chemical constituents of the ethanolic extract of Mikania parodii. Chem Nat Compd 44:512-513.         [ Links ]

Guisalberti EL 1997. The biological activity of naturally occurring kaurane diterpenes. Fitoterapia 23:303-325.         [ Links ]

Gutierrez AB, Herz W 1988. Bisabolones and other constituents of Mikania shushunensis. Phytochemistry 27:3871-3874.         [ Links ]

Gutierrez AB, Oberti JC, Herz W 1988. Germacran-5,14,6,12diolides from Mikania urticifolia. Phytochemistry 27:938-939.         [ Links ]

Gutierrez AB, Oberti JC, Kulanthaivel P, Herz W 1985. Sesquiterpene lactones and diterpenes from Mikania periplocifolia. Phytochemistry 24:2967-2971.         [ Links ]

Gutierrez AR 1993. Pharmaceutical composition and method of treatment utilizing the composition/A composition comprising extracts of Abelmoscus moschatus and Mikania guaco for treating snakebite and scorpion sting. US 5229119 A 19930720        [ Links ]

Hasan SMR, Jamila M, Majumder MM, Akter R, Hossain M, Mazumder EH, Alam A, Jahangir R, Rana S, Rahman A, Rahman S 2009. Analgesic and antioxidant activity of the hydromethanolic extract of Mikania scandens (L.) Willd. leaves. Am J Pharmacol Toxicol 4:1-7.         [ Links ]

Herz W, Kulanthaivel P 1985. Diterpenes and sesquiterpene lactones from Mikania congesta. Phytochemistry 24:1761-1768.         [ Links ]

Herz W, Santhanam PS 1969. Isolation, structure and synthesis of 4',5,6,7-tetrahydroxy-3'-methoxyflavone (Batatifolin), a new flavone from Mikania batatifolia DC. Tetrahedron Lett 39:3419-3421.         [ Links ]

Herz W, Santhanam PS, Subramaniam PS, Schmid JJ 1967. The structure of mikanolide, a new sesquiterpene dilactone from Mikania scandens (L.) Willd. Tetrahedron Lett 32:3111-3115.         [ Links ]

Herz W, SrinivasanA, Kalyanaraman PS 1975. Mikanokryptin, a new guianolide from Mikania. Phytochemistry 14:233-237.         [ Links ]

Herz W, Subramaniam PS, Santhanam PS, Aota K, Hall AL 1970. Structure elucidation of sesquiterpene dilactones from Mikania scandens (L.) Willd. J Org Chem 35:1453-1464.         [ Links ]

Hoult JRS, Payá M 1996. Pharmacological and biochemical actions of simple coumarins: natural products with therapeutic potential. Gen Pharmacol 27:713-722.         [ Links ]

Huang H, Ye W, Wei X, Zhang C 2009. Allelopathic potential of sesquiterpene lactones and phenolic constituents from Mikania micrantha H.B.K. Biochem Syst Ecol 36:867-871.         [ Links ]

Huang H, Ye W, Wu P, Lin L, Wei X 2004. New sesquiterpene dilactones from Mikania micrantha. J Nat Prod 67:734-736.         [ Links ]

Hui YH, Ruprecht JK, Liu YM, Anderson JE, Smith DL, Chang CJ, McLaughlin JL 1989. Bullatacin and bullatacinone: two highly potent bioactive acetogenins from Annona bullata. J Nat Prod 52:463-467.         [ Links ]

Ismail BS, Chong TV 2002. Effects of aqueous extracts and decomposition of Mikania micrantha H.B.K. debris on selected agronomic crops. Weed Biol Manag 2:31-38.         [ Links ]

Jiang RW, He ZD, But PPH, Chan YM, Ma SC, Mak TCW 2001. A novel 1:1 complex of potassium mikanin-3-O-sulfate with methanol. Chem Pharm Bull 49:1166-1169.         [ Links ]

Jolad SD, Wiedhopf RM, Cole JR 1974. Tumor-inhibitory agent from Zaluzania robinsonii (Compositae). J Pharm Sci 63:1321-1322.         [ Links ]

Kiang AK, Sim KY, Goh J 1965. Constituents of Mikania cordata (burm. f.) B. L. Robinson (compositae). Isolation of mikanin, epifriedelinol, and fumaric acid; the structure of mikanin. J Chem Soc: 6371-6374.         [ Links ]

Kiang AK, Sim KY, Yoong SW 1968. Constituents of Mikania cordata (Burm. F.) B. L. Robinson (Compositae)-II. Phytochemistry 7:1035-1037.         [ Links ]

King RM, Robinson H 1987. The Genera of the Eupatorieae (Asteraceae). Monographs in Systematic Botany. Missouri Botanical Garden: St.Louis, MO. 419p.         [ Links ]

Leite MGR, Souza CL, Silva MAM, Moreira LKA, Matos FJA, Viana GSB 1993. Estudo farmacológico comparativo de Mikania glomerata Sprengel (guaco), Justicia pectoralis Jacq (anador) e Torresea cearensis (cumaru). Rev Bras Farm 74:12-15.         [ Links ]

Limberger R, Suyenaga ES, Henriques ATC, Menut C, Verin P, Lamaty G, Bessiere JM 1998. Aromatic plants from Brazil. Part VI. Chemical composition of essential oils from three southern Brazilian species of Mikania (Asteraceae). J Essent Oil Res 10:363-367.         [ Links ]

Lobitz GO, Tamayo-Castillo G, Merfort I 1997. Diterpenes and sesquiterpenes from Mikania banisteriae. Phytochemistry 46:161-164.         [ Links ]

Lobitz GO, Tamayo-Castillo G, Poveda L, Merfort I 1998. Kaurene diterpenes from Mikania vitifolia. Phytochemistry 49:805-809.         [ Links ]

Lorenzi H, Matos FJA 2002. Plantas medicinais no Brasil Nativas e Exóticas. Nova Odessa: Instituto Plantarum. 544p.         [ Links ]

Maiorano VA 2005. Antiophidian properties of the aqueous extract of Mikania glomerata. J Ethnopharmacol 102:364-370.         [ Links ]

Marcus DM, Grollman AP 2002. Botanical medicines - The need for new regulations. New Engl J Med 347:20732076.         [ Links ]

Mathur SB, Fermin CM 1973. Terpenes of Mikania mongenansis. Phytochemistry 12:226-227.         [ Links ]

Mathur SB, Tello PG, Fermin CM, Mora-Arellano Y 1975. Terpenoids of Mikania monagasensis and their biological activities. Rev Lat Am Ing Quím 6:201-205.         [ Links ]

Matsuda H, Kagerura T, Toguchida I, Ueda H, Morikawa T, Yoshikawa M 2000. Inhibitory effects of sesquiterpenes from bay leaf on nitric oxide production in lipopolysaccharide-activated macrophages:structure requirement and role of heat shock protein induction. Life Sci R 66:2151-2157.         [ Links ]

Mosaddik MA, Alam KMF 2000. The anti-ulcerogenic effect of an alkaloidal fraction from Mikania cordata on Diclofenac sodium-induced gastrointestinal lesions in rats. J Pharm Pharmacol 52:1157-1162.         [ Links ]

Morton JF 1981. In Thomas CC (Ed), Atlas of Medicinal Plants of Middle America, Bahamas to Yucatan. Springfield, 947-948.         [ Links ]

Moura SR, Costa SS, Jansen JM, Silva CA, Lopes CS, Bernardo-Filho M, Silva VN, Criddle DN Portela BN, Rubenich LMS, Araújo RG, Carvalho CRM 2002. Bronchodilator activity of Mikania glomerata Sprengel on human bronchi and guinea-pig trachea. J Pharm Pharmacol 54:249-256.         [ Links ]

Nascimento AM, Chaves JS, Albuquerque S, Oliveira DCR 2004. Trypanocidal properties of Mikania stipulacea and Mikania hoehnei isolated terpenoids. Fitoterapia 75:381-384.         [ Links ]

Nascimento AM, Oliveira DCR 2001. Kaurene diterpenes and other chemical constituents from Mikania stipulacea (M. Vahl) Willd. J Brazil Chem Soc 12:552-555.         [ Links ]

Nicollier G, Thompson AC 1981. Essential oil and terpenoids of Mikania micrantha. Phytochemistry 20:2587-2588.         [ Links ]

Nunez CV, Amêndola MC, Lago JHG, Roque NF 2004. Diterpene acids from Mikania sp. nov (Asteraceae). Biochem Syst Ecol 32:233-237.         [ Links ]

Oguntimein BO 1987. The terpenoids of Annona reticulata. Fitoterapia 58:411-413.         [ Links ]

Ohkoshi E, Kamo S, Makino M, Fujimoto Y 2004. Entkaurenoic acids from Mikania hirsutissima (Compositae). Phytochemistry 65:885-890.         [ Links ]

Oliveira FD 1972. Contribuição para o estudo botânico de Mikania hirsutissima DC. var.hirsutissima. II. Morfologia externa e anatomia da folha, flor, fruto e semente. Rev Farm Bioquim 10:15-36.         [ Links ]

Oliveira F, Alvarenga MA, Akisue G, Akisue MK 1984. Isolamento e identificação de componentes químicos de Mikania glomerata Sprengel e de Mikania laevigata Schultz Bip. ex Baker. Rev Farm Bioquim 2:169-183.         [ Links ]

Oliveira F, Oga S, Akisue G, Akisue MK 1985. Parâmetros físicos e químicos e efeito antiedema dos extratos fluidos de guaco (Mikania glomerata Sprengel) e de guaco de mato (Mikania laevigata Schutz Bip. ex Baker). An Farm Quim São Paulo 25:50-54.         [ Links ]

Oliveira PA, Gregorio LE, Oliveira DCR 2007. Comparative analysis of sesquiterpene lactones from Mikania cordifolia collected from three different locations. Chem Nat Compd 43:140-142.         [ Links ]

Oliveira PA, Turatti ICC, Oliveira DCO 2006. Comparative analysis of triterpenoids from Mikania cordifolia collected from four different locations. Rev Bras Cienc Farm 42:547-552.         [ Links ]

Paul RK, Jabbar A, Rashid MA 2000. Antiulcer activity of Mikania cordata. Fitoterapia 71:701-703.         [ Links ]

Peluso G, De Feo V, De Simone F, Bresciano E, Vuotto ML 1995. Studies on the inhibitory effects of caffeoylquinic acids on monocyte migration and superoxide ion production. J Nat Prod 58:639-649.         [ Links ]

Pereira BMR, Gonçalves LC, Pereira NA 1992. Abordagem farmacológica de plantas recomendadas pela medicina folclórica como antiofídicas III. Atividade antidermatogênica. Congresso Brasileiro de Plantas Medicinais. Curitiba: Universidade Federal do Paraná, p. 1.         [ Links ]

Pereira NA, Pereira BMR, Nascimento MC, Parente JP, Mors WB 1994. Pharmacological screening of plants recommended by folk medicine as anti-snake venom. IV. Proction against jararaca venom by isolated constituints. Planta Med 60:99-100.         [ Links ]

Pessini GL, Holetz FB, Sanches NR, Cortez DAG, Dias-Filho BP, Nakamura CV 2003. Avaliação da atividade antibacteriana e antifúngica de extratos de plantas utilizados na medicina popular. Rev Bras Farmacogn 13:21-24.         [ Links ]

Picman AK 1986. Biological activities of sesquiterpenes lactones. Biochem Syst Ecol 14:255-281.         [ Links ]

Pio Correa M 1984. Dicionário das plantas úteis do Brasil e das exóticas cultivadas. 2 ed., Rio de Janeiro, 108-129.         [ Links ]

Prevost G, Coulomb H, Lavergne O, Lanco C, Teng B-P 2002. Preparation of pharmaceutical compositions containing mikanolide, dihydromikanolide or an analog thereof combined with another anticancer agent for therapeutic use in cancer treatment PCT Int. Appl. WO 2002096348 A2 20021205.         [ Links ]

Quisumbing E 1978. Medicinal plants of the Phillippines. Quezon City: Katha Publications, 990p.         [ Links ]

Reis AA, Mendes CC, Ferraz TPL, Roque NF 2003. Terpenes from Mikania hookeriana. Biochem Syst Ecol 31:1061-1062.         [ Links ]

Rice EL 1984. Allelopathy. 2 ed. Academic Press, Orlando, Florida.         [ Links ]

Ritter MJ, Miotto STS 2005. Taxonomia de Mikania Willd. (Asteraceae) no Rio Grande do Sul, Brasil. Hoehnea 32:309-359.         [ Links ]

Rüngeler P, Brecht V, Tamayo-Castillo G, Merfort I 2001. Germacranolides from Mikania guaco. Phytochemistry 56:475-489.         [ Links ]

Ruppelt BM, Pereira EF, Gonçalves LC, Pereira NA 1991. Pharmacological screening of plants recommended by folk medicine as anti-snake venom:I. Analgesic and anti-inflammatory activities. Mem I Oswaldo Cruz 86:203-205.         [ Links ]

Salgado HRN, Roncari AFF, Moreira RRD 2005. Antidiarrhoeal effects of Mikania glomerata Spreng. (Asteraceae) leaf extract in mice. Rev Bras Farmacogn 15:205-208.         [ Links ]

Santos SC, Krueger CL, Steil AA, Krueger MR, Biavati MW, Wisnievski-Junior A 2006. LC characterization of guaco medicinal extracts, Mikania laevigata and M. glomerata, and their effects on allergic pneumonitis. Planta Med 72:679-684.         [ Links ]

Serrano SM, Nogal JJ, Diaz MRA, Escario JA, Fernández AR, Barrio AG 2000. In vitro screening of American plant extracts on Trypanosoma cruzi and Trichomonas vaginalis. J Ethnopharmacol 71:101-107.         [ Links ]

Silva MIG, Gondim APS, Nunes IFS, Sousa FCF 2006. Utilização de fitoterápicos nas unidades básicas de atenção à saúde da família no município de Maracanaú (CE). Rev Bras Farmacogn 16:455-462.         [ Links ]

Silva ML, Luz AIR, Zoghbi MGB, Ramos LS, Maia JGS 1984. Essential oils of some Amazonian Mikania species. Phytochemistry 23:2374-2376.         [ Links ]

Soares AP, Nascimento AM, Taleb-Contini SH, Oliveira DCR 2007. Constituents of Mikania lasiandrae. Chem Nat Compd 43:708-709.         [ Links ]

Souza JM, Taleb-Contini SH, Oliveira DCR 2006. Phytochemical study of Mikania pseudohoffmanianna G.M. Barroso ex W. C. Holmes. Rev Bras Cien Farm 42:265-268.         [ Links ]

Suyenaga ES, Reche E, Farias FM, Schapoval EES, Chaves CGM, Henriques AT 2002. Antiinflammatory investigation of some species of Mikania. Phytother Res 16:519-523.         [ Links ]

Teng BP, Prevost G, Brezak M-C P, Moumen M 2001. Novel DNA polymerase inhibitors, mikanolide and dihydromikanolide PCT Int. Appl. WO 2001039720 A2 20010607.         [ Links ]

Teske M, Trentini AM 1997. Compêndio de Fitoterapia Herbarium. 3 ed. Curitiba: Herbarium.         [ Links ]

Vicentino ARR, Menezes FS 2007. Atividade antioxidante de tinturas vegetais, vendidas em farmácias com manipulação e indicadas para diversos tipos de doenças pela metodologia do DPPH. Rev Bras Farmacogn 17:384-387.         [ Links ]

Vichnewski W, Filho HFL, Murari R, Herz W 1977. Cinnamoylgrandifloric acid from Mikania oblongifolia. Phytochemistry 16:2028-2029.         [ Links ]

Vidal LHI, Souza JRP, Fonseca EP, Bordin I 2006. Qualidade de mudas de guaco produzidas por estaquia em casca de arroz carbonizada com vermicomposto. Hortic Bras 24:26-30.         [ Links ]

Vieira SH, Takahashi JA, Oliveira AB, Chiari E, Boaventura MA 2002. Novel derivatives of kaurenoic acid:preparation and evaluation of their trypanocidal activity. J Brazil Chem Soc 13:151-157.         [ Links ]

Wei X, Huang H, Wu P, Cao H, Ye W 2004. Phenolic constituents from Mikania micrantha. Biochem Syst Ecol 32:1091-1096.         [ Links ]

Wilkens M, Alarcon C, Urzua A, Mendoza L 2002. Characterization of the bactericidal activity of the natural diterpene kaurenoic acid. Planta Med 68:452-454.         [ Links ]

Yatsuda R, Rosalen PL, Cury JA, Murata RM, Rehder VLG, Melo VL 2005. Effects of Mikania genus plants on growth and cell adherence of mutans streptococci. J Ethnopharmacol 97:83-89.         [ Links ]

Yoshikawa M, Shimoda H, Uemura T, Morikawa T, Kawahara Y, Matsuda H 2000. Alcohol absorption inhibitors from bay leaf (Laurus nobilis): structure-requirements of sesquiterpenes for the activity. Bioorg Med Chem Lett 8:2071-2077.         [ Links ]

Ysrael MC, Croft KD 1990. Inhibition of leukotriene and platelet activating factor synthesis in leukocytes by the sesquiterpene lactone scandenolide. Planta Med 56:268-270.         [ Links ]

Zamorano G, Catalán CAN, Díaz JG, Herz W 1994. Germacranolides and sesquiterpene dilactones from Mikania dusenii. Phytochemistry 37:187-190.         [ Links ]

Zamorano G, Catalán CAN, Díaz JG, Herz W 1995. Sesquiterpene dilactones from Mikania ypacarayensis. Phytochemistry 38:1257-1260.         [ Links ]



Sidnei Moura Instituto de Biotecnologia
Universidade de Caxias do Sul
95070-560 Caxias do Sul-RS, Brazil
Tel.:+55 54 3218 2149

Received 19 Mar 2012
Accepted 4 Jun 2012

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License