SciELO - Scientific Electronic Library Online

vol.23 número1Estudo da resistência tênsil da parede abdominal após síntese de laparotomia usando três tipos de fios cirúrgicos em ratos WistarModelo experimental de tumor de pulmão em rato por via intrabrônquica índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados




Links relacionados


Acta Cirurgica Brasileira

versão impressa ISSN 0102-8650versão On-line ISSN 1678-2674

Acta Cir. Bras. v.23 n.1 São Paulo jan./fev. 2008 



Incorporation by host tissue of two biomaterials used as repair of defects produced in abdominal wall of rats1


Incorporação por tecido do hospedeiro de dois biomateriais usados como reparo de defeitos produzido em parede abdominal de ratos



Suyiene Cordeiro FalcãoI; Joaquim Evêncio NetoII; Antônio Roberto de Barros CoelhoIII

IPhD, Veterinary Sciences, Department of Veterinary Medicine, Federal Rural University of Pernambuco, Brazil
IIPhD, Associate Professor, Department of Animal Morphology and Physiology, Federal Rural University of Pernambuco, Brazil
IIIPhD, Associate Professor, Department of Surgery, Federal University of Pernambuco, Brazil





PURPOSE: Biomaterials may be used as treatment of great abdominal wall defects to avoid tension during repair. In the present research we intended to investigate incorporation type by host tissue of membranes of microbial cellulose (MC), produced by the bacteria Zoogloea sp., and of polytetrafluoroethylene (ePTFE) in abdominal wall defects of rats.
METHODS: Sixty male rats Wistar, anesthetized by ketamine (5mg/100g) and xylazine (2mg/100g), were submitted to a rectangular excision (2x3cm) of the abdominal wall, including fascia, muscles and peritoneum and further treated with implants of microbial cellulose (MC Group - 30 animals) or expanded polytetrafluoroethylene ( ePTFE Group- 30 animals). Each group was subdivided in 14th DPO, 28th DPO and 60th DPO Subgroups.
RESULTS: Incorporation of biomaterials was observed by wrapping and infiltration by host tissue. It has been found that wrapping associated to infiltration of host connective tissue in implants of ePTFE were present in 100% of the observed samples, and this may be responsible for increase resistance to traction. Inversely, wrapping without host tissue infiltration was seen in 100% of examined specimens of MC implants.
CONCLUSION: Wrapping and host tissue infiltration is seen only in ePTFE implants.

Key-words: Microbial cellulose. Zoogloea sp. Expanded polytetrafluoroethylene. Incorporation. Abdominal defect. Rats.


OBJETIVO: Biomateriais podem ser usados como tratamento de grandes defeitos da parede abdominal para evitar tensão durante reparo. Na presente pesquisa pretendeu-se investigar o tipo de incorporação pelo tecido do hospedeiro de membranas de celulose microbiana (CM), produzidas pela bactérias Zoogloea sp., e de politetrafluoretileno (PTFEe) em defeitos da parede abdominal de ratos.
MÉTODOS: Sessenta ratos machos Wistar, anestesiados através de cetamina (5mg/100g) e xilazina (2mg/100g), foram submetidos a uma excisão retangular (2x3cm) da parede abdominal, incluindo fascia, músculos e peritoneum e posteriormente tratadas com implantes de celulose microbiana (Groupo CM - 30 animais) ou politetrafluoretileno (Grupo PTFEe - 30 animais). Cada grupo foi subdividido em Subgrupos14º DPO, 28º DPO e 60º DPO.
RESULTADOS: Incorporação do biomaterial foi observada através de envoltório e infiltração pelo tecido do receptor. Foi encontrado que o envoltório associado à infiltração de tecido conjuntivo do hospedeiro em implantes de ePTFE estava presente em 100% das amostras observadas, podendo ser responsável por aumento da resistência à tração. Inversamente, envoltório sem infiltração de tecido do hospedeiro foi visto em 100% dos espécimes examinados nos implantes de CM.
CONCLUSÕES: Pode-se ser concluído que o envoltório associado à infiltração de tecido do hospedeiro só é vista nos implantes de PTFEe.

Descritores: Celulose microbiana. Zoogloea sp. Politetrafluoretileno expandido. Incorporação. Defeito abdominal. Ratos.




In some instances where reconstruction of muscle-aponeurotics defects is affected by great distance among its edges or by lack of tissue with proper characteristics for an appropriate approach, synthetic and biological implants, or even muscular grafts, vascularized or not, can be used for tissue repair 1.

In Veterinary Medicine, few experimental reports for clinical applications were found on the use of biocelulose produced by Acetobacter xylinum such as; cuff protection in the reconstruction of peripheral nerves2, healing of experimental wounds in mammary teats of bovine3, experimental teguments wounds in equine4 and swine5, prophylaxis of the occurrence of membrane after laminectomy in dogs6 and healing of experimental incisional lesions of the cornea in canine7.

The first clinical application of membranes of biocelulose produced by microorganism Zoogloea sp., in gross state, was done in UFRPE to treat natural cutaneous wounds of dogs. The results suggested control of the infection, accelerated growth of granulation tissue and abbreviation of healing time, when compared with the conventional treatment (antiseptic + cicatricial ointments) 8,9. In this work, the authors, possibly took advantage of the beneficial properties of sugar, main constituent of the wrapping of gross membranes (sugar-cane molasses), on wound healing. Studies of biocompatibility and citotoxity of the microbial cellulose produced by Zoogloea sp. were previously done, and authorize the accomplishment of experimental research for clinical application 10,11,12,13.

The use of cellulose membranes produced by the Zoogloea sp. or by other bacterial species, as repair of muscle-aponeurotics defects of the abdominal wall of animals, in experimental or clinical scope, has not been reported.

The present work has the objective to compare the use of membrane of microbial cellulose (MC) produced by the Zoogloea sp. and synthetic membrane of expanded polytetrafluoroethylene (ePTFE), as repair of produced defect in the abdominal wall of rats, through observation of incorporation of implanted material by host tissue.



Materials used as implants

The exopolyssacharide pellicle was produced by bacteria Zoogloea sp., isolated by the Institute of Antibiotics of the Federal University of Pernambuco, in static culture, having sugar-cane molasses as nutritious medium14. During the treatment process, the membrane was purified in solution of sodium hypochlorite (NaOCl) followed by several rinse sessions, mechanical compression and evaporation at the air, conditioned in polypropylene envelope immerged in solution of isopropyl alcohol moisturized at 20%, and finally sterilized in g rays*.

The membrane of expanded polytetrafuoroethylene (ePTFE) was obtained from vascular prostheses with internal diameter of 8mm and wall thickness of 0,8mm, with pores size of 25µm, cutting in the longitudinal direction, after removal of the external helical structure. Rectangles of 2x3cm were prepared, then conditioned in polypropylene envelopes and submitted to the sterilization in g rays.

Groups and Subgroups

The animals were distributed in two groups:

Microbial Cellulose Group (MC Group): composed of 30 animals that were submitted to a muscle-aponeurotic defect on ventral wall of abdomen and treated with membrane of microbial cellulose;

Expanded Polytetrafuoroethylene Group (ePTFE Group): composed of 30 animals that were submitted to a muscle-aponeurotic defect on ventral wall of abdomen and treated with membrane of expanded polytetrafluoroethylene;

Each group was subdivided in three Subgroups of 10 rats, in agreement with the postoperative day (POD) observation, being denominated of 14th POD Subgroup, 28th POD Subgroup and 60th POD Subgroup.


Sixty male Wistar rats, with mean weight of 437, 7g±40, 9, were housed in appropriate cages, fed with proper ration and mineral water ad libitum.

Anesthetic and surgical procedure

The animals were anesthetized with a mixture of ketamine (5mg/100g) and xylazine (2mg/100g) by intramuscular route, for accomplishment of a middle abdominal incision (5cm), proceeded by a rectangular excision (2x3cm) including fascia, muscles and peritoneum and then treated with implants of membranes of microbial cellulose or expanded polytetrafluoroethylene. At the days programmed for evaluations, under intraperitonial administration of sodium thiopental§ and, subsequently lethal doses of this barbiturate, the animals were submitted to the euthanasia for accomplishment of the histological exams.

Biopsy and stains

A segment corresponding to the implant/host interface, with 0, 5 cm of width, embracing the cranial extension of the sample, free from suture, was excised and immerged in buffered solution of formalin at 10%. After fixation of the samples, they were included in paraffin, sectioned and stained by hematoxylin-eosin (H-E) and Tricromic of Masson.

Histological observations

Observations were made on the presence of Wrapping without Infiltration in the Implants and Wrapping with Infiltration in the Implants, at 14th POD, 28th POD and 60th POD Subgroups, in rats of the MC and ePTFE Groups.



Microbial Cellulose Group (type of incorporation)

The observation of the specimens stained by H-E and Tricromic of Masson, in14th DPO, 28th DPO and 60th DPO Subgroups of MC Group revealed a type of incorporation characterized by presence of wrapping without infiltration in implants in 100% of the sample readings (Table 1, Figure 1).



Expanded Polytetrafluoroethylene Group (type of incorporation)

Using the same stains, in 14th DPO, 28th DPO and 60th DPO Subgroups of the ePTFE Group, the type of incorporation was represented by wrapping with infiltration in implants in 100% of the occasions (Table 2, Figure 2).




Incorporation of microbial cellulose implants

The membrane of microbial cellulose (MC) produced by the Zoogloae sp. has been considered as non porous material and incorporation of implant is achieve by capsulation (wrapping) 13. In a different way of ePTFE the cellulose produced by the Zoogloae sp. did not permitted invasion of fibroblasts in experimental repair of arteries and veins (angioplasties)15, as seen in the present report (Table 1, Figure 1)

Studies on biocompatibility through inclusion of MC, produced by the Acetobacter xylinum, in the abdominal musculature of rats, emphasizes the evolutionary histological composition of the wrapping (capsulation), from acute inflammatory phase (neutrofils) until the regenerative phase (fibroblasts and collagen synthesis), in a period of 28 days, without reference of infiltration of host tissue in the implant16. These authors also report the presence of giant cells with progressive character until 28th DPO.

However, in another study accomplished with microbial cellulose produced by Acetobacter xylinum subsp. sucrofermentans, in static culture, the obtained pellicles consisted of a flexible porous net of nanofibrils, not joined; whose capacity to retain water was 99%. The net of fibrils was examined by scanning electronic microscopy (SEM) and described as denser in the interface between the culture medium and the air (compact side) and more porous on the opposite side (porous side). To the end of 12 weeks, on the porous side, the fibroblasts were completely integrated inside the structure of the membrane of MC, having synthesized collagen17. The same authors used a film of MC with retention 99% of water, allowing in this way infiltration of cells and proliferation of smooth muscular tissue in the spaces among fibrils, in a process of tissue engineering for construction of blood vessels. Through SEM, cells could be seen on the porous side, moving away the nanofibrils when the migration took place inside of the net of MC fibrils. The maximum infiltration depth into the MC observed after 1 week was of 20 µm. An infiltration depth into CM of up to 40 µm could be seen after two weeks in culture. To facilitate the growth of cells inside of the MC, they mention a technique to create wider spaces in the membrane, with the use of paraffin spheres, followed by removal of the same ones with solution of NaOH18.

In the sense to obtain a product that allows better incorporation with host tissue, multiperforated cellulose membrane is now being tested at the Núcleo de Cirurgia Experimental.

Incorporation of ePTFE implants

Reports on incorporation of ePTFE implants used as implants revealed a fibrous tissue firmly adherent to the surface of this material (wrapping). Fibroblasts were found in its interstice. The presence of these cells in the empty spaces of the structure of the polymer propitiates the synthesis of collagen, resulting in a stable and resistant repair19. These results are in agreement with our findings (Table 2, Figure 2).The depth reached by the infiltration of connective tissue within the ePTFE was evaluated in about 200 µm, in a period of five months19. In experimental angioplasties, the tissue invasion observed within the ePTFE patches has been described and occurred because they present an appropriated porous size (25 µm), which allow cellular migration15. The infiltration of cells and tissue is a process limited by the pore size of ePTFE, not being observed tissue infiltration in pores under 10 µm in diameter20.

Wrapping associated to infiltration of recipient tissue seen in ePTFE implants, as compared with microbial cellulose (MC) may be responsible for increased resistance to traction at the interface implant/host 21.



Incorporation composed by wrapping associated to host tissue infiltration is seen only in ePTFE implants.



1. Santillán-Doherty P, Jasso-Victoria R, Sotres-Vega A, Olmos R, Arreda J, Garcia D, Gaxiola M. Reparacíon de defectos de pared tóracoabdominal de perros com bioprótesis de pericárdio bovino. Rev Invest Clin.1995; 47(6):43946.        [ Links ]

2. Torres M F P, Graça D L, Farias E L P. Reparação microcirúrgica de nervo periférico por meio de sutura, cola de fibrina ou bainha de BioFill® em ratos Wistar. Arq Bras Med Vet e Zootec. 2003; 55 (5):557-61.        [ Links ]

3. Marques J A, Moraes J R E, Teixeira Neto F J. Tratamento alternativo de feridas de papilas mamárias de vacas através do emprego de membrana biológica (Biofill Produtos Biotecnológicos, Curitiba,PR). Braz J Vet Res Anim Sci. 1996; 33 (2):102-6.        [ Links ]

4. Vaz B B D, Marques J A, Moraes J R E. Avaliação microscópica da evolução cicatricial de feridas cutâneas induzidas experimentalmente na espécie eqüina (Equus caballus), tratadas ou não com película de celulose. Ars Veterinária. 1997;13 (1):17-27.        [ Links ]

5. Wouk A F P F, Diniz J M, Círio S M, Santos H, Baltazar E L, Acco A. Membrana biológica (Biofill®). Estudo comparativo com outros agentes promotores da cicatrização da pele em suínos: aspectos clínicos, histopatológicos e morfométricos. Arch Vet Scienc. 1998; 3 (1):31-7.        [ Links ]

6. Costa R C, Pippi N L, Graça D L, Fialho S A, Alves A, Groff A C et al. The effects of free fat graft or cellulose membrane implants on laminectomy membrane formation in dogs. Vet J. 2006; 171 (3):491-9.        [ Links ]

7. Schoenau L S F, Pippi N L, Schossier J E V. Avaliação clínica preliminar do fechamento comparativo de incisões corneanas com sutura e Biofill (Película Celulósica). Ciência Rural. 1993; 23(2):173-7.        [ Links ]

8. Melo F A D, Coelho M C O C,  Ferreira V M, Monteiro V L, Carrazone P G. Biopolímero produzido a partir da cana de açúcar para cicatrização cutânea. In: VII CONGRESSO STAB. 1999, Londrina. Anais do VII Congresso STAB. Londrina, p. 251-5.        [ Links ]

9. Coelho M C O C, Carrazoni P G, Monteiro V L C, Melo F A D, Mota R A, Tenório Filho F. Biopolímero produzido a partir da cana-de-açúcar para cicatrização cutânea. Acta Cir Bras. 2002; 17 (supl. 1):11-3.        [ Links ]

10. Andrade C E M C, Aguiar J L A. Biopolímero da cana-de-açúcar: estudo de biocompatibilidade. In: X CONGRESSO DE INICIAÇÃO CIENTÍFICA (CONIC/ CNPQ/UFPE), v II, 2002. Recife. Anais do X Congresso de Iniciação Científica (CONIC/CNPQ/UFPE), Editora Universitária, 2002, v. II, p. 191.        [ Links ]

11. Castro C M M B, Aguiar J L A, Melo F A D, Silva W T F, Marques E, Silva D B. Citotoxidade de biopolímero de cana-de-açúcar. An Fac de Med Univ Fed Pernamb. 2004; 49 (2):119-23.        [ Links ]

12. Lima F R, Aguiar J L A Biocompatibilidade comparada de gel de exopolisacarídeo de melaço cana-de-açúcar e de gel de polimetilacrilato com gordura autóloga. In: XIII CONGRESSO DE INICIAÇÃO CIENTÍFICA (CINIC/CNPQ/UFPE), outubro de 2005. Recife. Anais do XIII Congresso de Iniciação Científica (CONIC/CNPQ/UFPE), Recife. 2005. [CD-ROM].        [ Links ]

13. Lima F R, Lima J R A, Hirakava P, Medeiros Jr. M D, Lima F M T, Aguiar J L A. Resposta inflamatória a biomembranas de polímero de cana-de-açúcar e telas de polipropileno® implantadas no peritônio parietal de ratos. An Fac de Med Univ Fed Pernamb. 2005; 50 (1): 37-9.        [ Links ]

14. Paterson-Beedle M, Kennedy J F, Melo F A D, Lloyd L L, Medeiros. A cellulosic exopolysaccharide produced from sugarcane molasses by a Zoogloea sp. Carbohydr Pol. 2000; 42(4):37583.        [ Links ]

15. Aguiar J L A, Lins E M, Marques S R B, Coelho A R B, Rossiter R O, Melo R J V. Sugarcane biopolymer patch in femoral artery angioplasty on dogs. Acta Cir Bras. 2007; (22) (Suppl 1):77-81.        [ Links ]

16. Queiroz V F, Silvado R A B, Simões M J, Goldenberg S. Aspectos morfológicos e morfométricos da reação tecidual a película celulósica introduzida no plano muscular da parede abdominal. Acta Cir Bras. 1989; 4(4):144-8.        [ Links ]

17. Helenius G, Bäckdahl H, Bodin A, Nannmark K U, Gatenholm P, Risberg B. In vivo biocompatibility of bacterial cellulose.J Biomed Mat Res Part A. 2005; 76A (2):431-8.        [ Links ]

18. Bäckdahl H, Helenius G, Bodin A, Nannmark K U, Johansson B R, Risberg B, Gatenholm P. Mechanical properties of bacterial cellulose and interactions with smooth muscle cells. Biomaterials. 2006; 27( 9):2141-9.        [ Links ]

19. Bauer, J J, Salky B A, Gelernt I M, Kreel I. Repair of large abdominal defects with expanded polytetrafluoroethylene (PTFE). Ann Surg. 1987;206(6):765-9.        [ Links ]

20. Kafejian-Haddad A P, Haddad-Filho D, Guidugli-Neto J, Goldenbeg S. Estudo comparativo das reações teciduais de silicone e politetrafluoroetileno expandido no dorso de ratos. Acta Cir Bras. 1997; 12 (3):182-8.        [ Links ]

21. Falcão S C. Membranas de celulose microbiana (Zoogloea sp.) e de politetrafluoretileno expandido usadas na reconstrução de defeitos produzidos na parede abdominal de ratos. Estudo comparativo. [Tese de Doutorado]. Universidade Federal Rural de Pernambuco - Centro de Ciências da Saúde-Departamento de Medicina Veterinária.; 2007.        [ Links ]



We thank Professor Sílvio Romero Marques who gently provided ePTFE material.

This work was support by Brazilian Government scholarship from CAPES (Coordenação de Aperfeiçoamento de Nível Superior).



Antônio Roberto de Barros Coelho
Rua Galvão Raposo, 234
50610-330 Recife-PE, Brazil
Phone /Fax: (55 81) 3227-0142

Received: September 28, 2007
Review: November 21, 2007
Accepted: December 12, 2007
Conflict of interest: none
Financial support: In acknowledgment



1 Research performed at Laboratory of Experimental Surgery, Department of Surgery- Federal University of Pernambuco, Brazil.
* Department of Nuclear Energy, Federal University of Pernambuco
Ketalar 50mg. Cristalia Laboratories of Brazil
Rompum 20mg. Bayer Laboratories of Brazil
§ Thiopentax, Cristalia Laboratories of Brazil

Creative Commons License Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons