Development of an experimental model of neurocysticercosis-induced hydrocephalus. Pilot study¹

ABSTRACT

PURPOSE:

To develop an experimental model of neurocysticercosis-induced hydrocephalus

METHODS:

There were used 17 rats. Ten animals were inoculated with Taenia crassiceps cysts into the subarachnoid. Five animals were injected with 0.1ml of 25% kaolin (a standard solution for the development of experimental hydrocephalus) and two animals were injected with saline. Magnetic resonance imaging (MRI) was used to evaluate enlargement of the ventricles after one or three months of inoculation. Volumetric study was used to quantify the ventricle enlargement.

RESULTS:

Seven of the 10 animals in the cyst group developed hydrocephalus, two of them within one month and five within three months after inoculation. Three of the five animals in the kaolin group had hydrocephalus and none in the saline group. Ventricle volumes were significantly higher in the 3-months MRI cyst subgroup than in the 1-month cyst subgroup. Differences between cyst subgroups and kaolin group did not reach statistical significance.

CONCLUSION:

The developed model may reproduce the human condition of neurocysticercosis-related hydrocephalus, which exhibits a slowly progressive chronic course.

Key words:
Neurocysticercosis; Hydrocephalus; Magnetic Resonance Imaging; Cerebrospinal Fluid; Rats.

Introduction

Neurocysticercosis is the most common parasitic disease of the central nervous system. The clinical manifestations of neurocysticercosis are pleomorphic and depend on the number and developmental stage of the parasite and site affected, as well as on the immune response of the patient^1,2. Cysts located in the basal cisterns and ventricles are associated with a more aggressive course. This condition is called extraparenchymal neurocysticercosis and can manifest as vasculitis, cysticercal meningitis, hydrocephalus and intracranial hypertension^3,4.

Hydrocephalus is the result of chronic inflammatory arachnoiditis or mechanical obstruction of cerebrospinal fluid (CSF) flow^4-6. The conditions occurs in about 30% of patients with neurocysticercosis and is associated with high rates of mortality^4,7. The treatment of choice in these cases is surgery consisting of the microsurgical removal of cysts, when possible, or CSF shunts⁴. Although CSF shunts are the most widely employed procedure, they are associated with several complications such as infection and dysfunction, which require reoperations^8,9. Thus, hydrocephalus is the malignant form of neurocysticercosis par excellence. Several studies of experimental neurocysticercosis focusing on immune responses and on parasite-host interactions have been published^10,11 but none of them focused on hydrocephalus.

The complexity of the relation between host and parasite, modulated by use of corticoids in clinical practice, make it difficult to fully understand the pathophysiologic mechanisms responsible for determining hydrocephalus in neurocysticercosis. This way, an animal model to help understand the inflammatory response is critical.

The aim of the present study was to develop an experimental model of neurocysticercosis-induced hydrocephalus in an attempt to reproduce a common and challenging clinical problem in the laboratory.

Methods

The animals were handled according to ethical guidelines and current legislation. The board of the local Ethics Committee on Animal Use approved the project.

Seventeen adult Wistar rats (Rattus norvegicus) weighing 150 to 250g were used. The animals were kept under adequate sanitary conditions in a temperature-controlled room (21^°C) under light-dark control. Food and water were available ad libitum.

Parasites and inoculation

Taenia crassiceps cysts were maintained in the peritoneal cavity of mice (Mus musculus). After aseptic removal, the cysts were injected into the cisterns of rats by suboccipital puncture with a 25G needle. All animals were submitted to general anesthesia by intraperitoneal injection of a mixture of ketamine (100 mg/ml) and xylazine (20 mg/ml) at a dose of 0.1 ml/kg.

Experimental groups

The animals were divided into three groups: cyst group (subarachnoid injection of 50 cysts, n = 10), kaolin group (subarachnoid injection of 0.1 ml of 25% kaolin - hydrated aluminum silicate, a standard solution to induce experimental hydrocephalus^12,13; n = 5), and sham group (subarachnoid injection of 0.1 ml of 0.9% saline, n = 2).

Radiologic assessment

Magnetic resonance imaging (MRI) using a 0.25 T equipment (Esaote model Vet-MR) was performed to verify the development of hydrocephalus. The protocol consisted of T2-weighted gradient echo acquisitions (0.6-mm thickness; T eco = 5 ms; T repetition = 10 ms). MRI was performed within one month after the procedure in the kaolin and sham groups and also in five animals of the cyst group. The other five animals of the cyst group were submitted to MRI three months after inoculation. The animals were euthanized after MRI. Ventricle volume was assessed using the ITK-SNAP software (version 3.0.0, USA) to determine the severity of hydrocephalus.

Statistical analysis

The statistical analysis was performed using the softwares SPSS version 21.0, R version 2.11.0 and GraphPad version 5.0. For comparison of ventricle volume we used the Kruskal-Wallis nonparametric test followed by the Dunn test for multiple comparisons. Differences were considered statistically significant if p<0.05.

Results

In the kaolin group, three of the five animals developed hydrocephalus (Figure 1A). Volumetric analysis in this group showed a median ventricle volume of 24.4 mm³. In the cyst group, seven of the ten animals developed hydrocephalus (Figure 1B and 1C), which was more evident in the subgroup of animals that underwent MRI three months after inoculation (5/5 animals, median ventricle volume = 120 mm³⁾ than in the subgroup submitted to MRI 1 month after inoculation (2/5 animals, median ventricle volume = 4.8 mm³⁾. No animal of the sham group had hydrocephalus (Figure 1D; mean ventricle volume = 3.0 mm³⁾. Ventricle volumes were significantly higher in the three-months MRI cyst subgroup than in the 1-month cyst subgroup. Differences between cyst subgroups and kaolin group did not reach statistical significance (p>0.05). The data are summarized in Table 1. We did not observe any changes on neurobehavioural patterns of cyst-inoculated animals through the experimental course period.

FIGURE 1
T2-weighted gradient echo MRI scans obtained for each experimental group. A: kaolin group; B: cyst group, MRI performed 1 month after inoculation; C: cyst group, MRI performed 3 months after inoculation; D: sham group. The selected images are from equivalent head positions. Enlarged ventricles are pointed with arrows. A and C show definite ventricle enlargement, whereas B shows mild ventricle enlargement. Ventricle is not visible on D, as the animal is not hydrocephalic

TABLE 1
Number of successful inductions of hydrocephalus and median ventricle volume in the experimental groups.

	Kaolin group (n = 5)	Cyst group		Sham group (n = 2)
		1 month MRI (n = 5)	3 months MRI (n = 5)
Number of hydrocephalic animals	3	2	5	0
Median ventricle volume (mm3)Range (mm3)	24.4 (1.0 - 692.0)	4.8* (0.4 - 40.2)	120.0* (49.0 - 437.0)	Mean: 3.0 (1.68 - 4.35)

• *Ventricle volumes were significantly higher in the 3-months MRI subgroup than in the 1-month MRI subgroup (p<0.05). The cutoff value of 5.0mm³ was set to consider a normal ventricle volume.14

Discussion

Human studies on the inflammatory response to neurocysticercosis are very limited because of the chronic progression of the disease, its different developmental stages, and the influence of administered corticosteroids¹⁰.

Since Taenia solium, the tapeworm that causes human neurocysticercosis, does not infect rodents in experimental assays¹¹, alternative parasites have been used. Taenia crassiceps is a tapeworm related to T. solium. Its definitive hosts are foxes, wolves and dogs, and its intermediate hosts are wild rodents¹⁵. The biological cycle of T. crassiceps resembles that of T. solium, a fact rendering it a useful alternative. Another parasite widely used as a model of experimental neurocysticercosis is Mesocestoides corti; however, this parasite tends to invade the brain parenchyma and to replicate in the CNS, so it does not exactly reproduce the biological behavior of T. solium in the CNS¹¹.

With respect to experimental neurocysticercosis, studies from the group of Teale^10,16 have focused on the type of cell-mediated immune response and on the mechanisms responsible for leukocyte migration across the blood-brain barrier and ependyma. The authors found that the immune response of mice to neurocysticercosis is mediated by gamma delta T lymphocytes guided by a Th1 pattern of response.¹⁰ Furthermore, junctional complex proteins and matrix metalloproteinases were found to be directly involved in leukocyte infiltration¹⁶.

Matos-Silva et al.¹⁷ analyzed general inflammatory responses in a mouse model of encephalitis after inoculation of T. crassiceps. The authors observed the occurrence of ventriculomegaly, but this finding was casual and was probably due to mechanical obstruction of the lateral ventricle since the ventriculomegaly observed was asymmetrical, shifting the midline.

Hydrocephalus in experimental neurocysticercosis has so far not been well documented. This may be due to the fact that all previously published experimental studies of neurocysticercosis injected cysts into the convex brain surface through a burr hole, which tends to mimic human parenchymal neurocysticercosis. We believed that it was necessary to inject the cysts into the CSF compartments in order to reproduce human extraparenchymal neurocysticercosis.

One may argue that our hydrocephalus results were due to mechanical obstruction of CSF outflow from the fourth ventricle to the subarachnoid space - it is, an obstructive hydrocephalus. We cannot rule out this possibility. However the fact that hydrocephalus was observed in only 2/5 animals within 1 month and in all animals after three months points to the possibility of a slowly progressing inflammatory reaction resulting in elevated resistance to CSF absorption, which favors the occurrence of arachnoiditis leading to hydrocephalus. This may reproduce the human condition of neurocysticercosis-related hydrocephalus, which exhibits an "arrested" course (a hydrocephalus that progresses very gradually and seems to be compensated)¹⁸.

The kaolin model has permitted significant advances in the understanding of the pathophysiology of hydrocephalus: ependymal disruption, periventricular edema, reactive astrogliosis, axon damage, corpus callosum flattening, myelin disintegration, and neuronal degeneration^13,19,20. Recently, aquaporin-4 and its upregulation in hydrocephalus have been suggested as an alternative pathway for CSF absorption and partial compensation of CSF imbalance^21-23. However in the kaolin-induced hydrocephalus model it is difficult to achieve control of the severity of hydrocephalus²⁴, as we have observed in our results from the kaolin group. Additionally, the classic kaolin model induces rapid enlargement of the brain ventricles, which differs from the "arrested" course of many patients seen in clinical practice. In this classic model, the animals have severe clinical manifestations such as loss of weight and severe neurobehavioural changes²⁵. To study an arrested course of hydrocephalus, Del Bigio et al.¹⁸ proposed a model of low-dose kaolin injection into immature rats. The authors achieved slow ventricle enlargement associated with pathological alterations similar to those seen in humans. However, another model of adult-onset slow progressive hydrocephalus is needed. Our model may have accomplished this goal, with the advantage of keeping animals healthy without noticeable neurobehavioural changes. Weekly repeated MRI scans of animals with neurocysticercosis-induced hydrocephalus could confirm if our model reproduces the arrested course of many patients. Also the use of contrast enhancement on MRI could highlight inflammatory reactions within CSF compartments. In the same way, analysis of CSF contents could demonstrate inflammatory changes.

Future investigation of our model will differentiate whether hydrocephalus is due to mechanical obstruction or to inflammatory responses in the basal cisterns, although both events occur in clinical practice. Finally, we will study histological and immunological features elicited by the presence of cysts in the CSF compartments.

Conclusion

The developed model may reproduce the human condition of neurocysticercosis-related hydrocephalus, which exhibits a slowly progressive chronic course.

Acknowledgements

To Dr. Diego Generoso for helping with animal procedures, Mr. Heraldo André Catalan Rosa for the support on magnetic resonance imaging acquisition, and Mr. Hélio Rubens de Carvalho Nunes for statistical analysis.

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• Financial source: none
• 1
  Research performed at Experimental Research Unit, Botucatu Medical School, Universidade Estadual Paulista (UNESP), Brazil. Part of Master degree thesis, Postgraduate Program in General Basis of Surgery, UNESP. Tutor: Marco Antônio Zanini.

Publication Dates

• Publication in this collection
  Dec 2015

History

• Received
  07 Aug 2015
• Reviewed
  11 Oct 2015
• Accepted
  13 Nov 2015