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Journal of the Brazilian Chemical Society

Print version ISSN 0103-5053

J. Braz. Chem. Soc. vol.14 no.4 São Paulo July/Aug. 2003

http://dx.doi.org/10.1590/S0103-50532003000400002 

REVIEW

 

Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions

 

 

Petr Skládal

Department of Biochemistry, Masaryk University, Kotlárská 2, 61137 Brno, Czech Republic

 

 


ABSTRACT

This review presents piezoelectric quartz crystals as transducers suitable for development of different types of bioanalytical assays. The components of measuring systems for piezosensors are described together with providers of commercial equipment. The piezoelectric biosensors are summarized for determination of viruses, bacterial and other cells, proteins, nucleic acids and small molecules as drugs, hormones and pesticides. In addition to mass changes, some agglutination assays employing viscosity effects are addressed. Finally, the direct label-free and real-time monitoring of affinity interactions using piezosensors is presented. The theoretical background for determination of appropriate kinetic rate and equilibrium constants is shown and the approach is demonstrated on the interaction of antibody with the corresponding antigen (protein secalin). Several examples of affinity studies are provided, including interactions of proteins (antibody and antigens, receptors and ligands), nucleic acids (hybridization, intercalation of metal complexes), lipids and saccharide-based layers.

Keywords: piezoelectric quartz crystal sensor, piezoelectric immunosensors, affinity interactions, kinetic parameters


RESUMO

Esta revisão apresenta cristais piezoeletricos de quartzo como transductores aplicáveis no desenvolvimento de diferentes tipos de ensaios bioanaliticos. Os componentes do sistema de medidas para sensores tipo piezo são descritos juntamente com os sistemas de equipamentos comerciais existentes. Os biosensores piezo elétricos podem ser aplicados para determinação de vírus, bacteriais e outras células, proteínas, ácidos nucléicos e pequenas moléculas encontradas em remédios, hormônios e pesticidas. Além de mudança de massa, alguns ensaios de aglutinação empregando efeitos de viscosidade são discutidos. Finalmente, o monitoramento da afinidade das interações direto sem-marcação e em tempo real usando sensores piezo é apresentado. Os pressupostos teóricos para a determinação apropriada das constantes de velocidade e de equilíbrio são apresentados e uma aproximação é demonstrada para a interação anticorpo com o correspondente antígeno (proteína secalin). Vários exemplos de estudo de afinidade são mostrados, incluindo a interação de proteínas (anticorpos e antígenos), de ácidos nucléicos (hibridização, intercalação de complexos metálicos), lipídeos e camadas com base de sacarídeos.


 

 

1. Introduction

Affinity interactions – formation of biocomplexes including antigen – antibody, protein – nucleic acid, hybridization of complementary strands of ssDNA, chemoreceptor – ligand etc., represent the most important biochemical processes involved in nature. This principle is also widely employed for design of bioanalytical assays. For this reason, a significant effort is devoted in detailed study and quantitative characterization of the underlying binding reaction. The direct methods for real-time monitoring of bioaffinity interactions employ much simplified assay formats compared to the traditional approaches based on labels as enzymes, fluorophores and radioactivity. The continuous evaluation of the progress of interaction provides more detailed information compared to label-based techniques, which measure the amount of the bound label only at the end of the binding process. In addition, the direct affinity sensors can be used repeatedly for many assays. Several physical transducers are capable of measuring surface mass changes resulting from the formation of biocomplexes at the sensitive area. Although mostly advanced optical systems are utilized, the piezoelectric and acoustic devices1 represent similar but significantly less expensive alternative. This review should provide a better insight into piezoelectric sensors with respect to bioanalytical applications.

Several anisotropic crystals exhibit piezoelectric effect - mechanical deformation of such crystals generates oriented dipoles and electric voltage. In the opposite, alternating voltage applied on such crystals excites vibrations. At resonance frequency equal to the natural frequency of vibration, transfer of energy from the electric field to the crystal is most efficient and the energy remains conserved in the oscillating system. The typical piezoelectric quartz crystal is shown together with scheme of the shear horizontal vibration (Figure 1). A thin plate of quartz (AT-cut) is coated with metal electrodes on both sides. The plate is usually inserted in a holder for simplified manipulation and connection to measuring systems.

 

 

The piezoelectric transducers are being used as chemical sensors since discovery of the relationship between mass of adsorbed rigid films and the resonant frequency changes by Sauerbrey:

The change Df of the resonant frequency f0 of the piezoelectric crystal is directly proportional to the mass change Dm, the numeric constant applies to calculations using Df in Hz, f0 in MHz and Dm in g/cm2. The system functions as a sensitive quartz crystal microbalance (QCM); improved sensitivity of recent devices resulted in the alternative name "nanobalance". In this way, the amount of molecules bound on the sensitive area of electrodes can be easily quantitatively measured as a decrease of the resonant frequency.

 

2. Measuring System for Piezosensors

The piezoelectric crystals with gold electrodes and the basic resonant frequency of 10 MHz are the most common ones, though crystals with higher frequencies (up to 27 MHz) will provide a higher sensitivity according to equation (1). Higher frequencies are not routinely used as the quartz plates become too thin and fragile. Piezoelectric crystals, oscillators and other instrumentation for microbalance might be obtained from several companies: International Crystal Manufacturing, Oklahoma City, OK (ICM, http://www.icmfg.com); Universal Sensors, Metairie, LA (http://intel.ucc.ie/sensors/universal/); Seiko / EG&G, Japan; Q-Sense, Göteborg, Sweden (http://www.q-sense.com); Elchema, Potsdam, NY; CH Instruments, Austin, TX (http://chinstruments.com). Comparison of other piezoelectric devices was published in the literature.3

Measuring setup for affinity studies consists of the piezosensor, driving electronics and a flow-through system (Figure 2). The crystal is usually sandwiched between two soft rubber orings; reasonable force should be applied to prevent damage of thin and brittle crystals. Alternatively, the crystal might be attached with the help of glue (silicon-based) either to the bottom or sidewall of a suitable vessel equipped with stirring bar. Holders for crystals suitable for immersion in solution are also available. For all cell designs, one side of the crystal should only contact the working solutions. The flow delivery system consist of a peristaltic pump providing flow rates around 30 to 200 mL min-1. Models with several rollers are preferred as the noise due to pulsation will be reduced. In addition, electronically controlled switching and/or multiposition valves and autosampler will enable automation of performance.

 

 

Oscillating circuit driving the crystal should provide enough energy to the crystal for smooth oscillation in the presence of liquid. The simplest constructions are based on the gate oscillator4 (the circuit 74LS01); the integrated form of this oscillator (74LS320, Figure 3) will provide much higher energy to the crystal resulting in improved performance under variable conditions.5 Better stability is obtained using carefully designed lever oscillators consisting of individual transistors (e.g. ICM).

 

 

A counter for measuring changes of the resonant frequency can be a common device widely used in electronics. This simplest direct counting method offers resolution of 1 Hz within 1 sec, typically for the 10 MHz crystals. To achieve better sensitivity, the output frequency from the oscillator is usually subtracted from a similar stable reference frequency and thus obtained low difference frequency signal (typically around 10 kHz) is further processed. One option (e.g. ELCHEMA) is to use a precise frequency to voltage converter, filter and fast high-resolution A/D converter to digitize the signal. Alternatively, the indirect counting approach can be chosen (e.g. CH Instruments) in which the lower experimental frequency is used as a clock signal for counting much higher stable frequency (tenths of megahertz). Both options provide a short measuring time (1 ms) and a resolution better than 0.1 Hz. Most commercial systems are connected to a personal computer in order to allow on-line monitoring of the affinity interaction.

 

3. Evaluation of Affinity Interactions

3.1 Theoretical model for determination of kinetic rate constants

In addition to concentration measurements and combined electrochemical experiments,6 the piezosensors are also suitable for kinetic characterization of affinity binding reactions. Modification of the sensing area with biomolecules provides affinity biosensors; the immobilized ligand A forms affinity complex with the complementary partner B in solution and the associated surface mass changes are evaluated. Formation of the biocomplex AB at the surface of the piezoelectric crystal is characterized by the kinetic association ka and dissociation kd rate constants:

The rate of formation of the complex d[AB]/dt can be expressed using the measured frequency f and concentration c for the free partner B:

The binding curves (f vs. t dependencies) were usually transformed to obtain df/dt vs. f plots that subsequently provide kinetic constants from equation ((2) using linear regression.7 A more elegant and precise method is integration of equation ((2) and then introduction of substitutions feq and kobs.8 The dependence of the resonant frequency f on time t can be fitted to the kinetic equation similarly as described for the optical biosensor systems:9

c is concentration of the free partner B and fmax represents the capacity of the crystal (maximum change of frequency obtained after saturation of all binding sites). In this way, the binding curves can be directly used for calculation of parameters using non-linear regression. Plot of kobs against concentration c provides values of the rate constants. A typical affinity experiment with the piezoelectric immunosensor is schematically shown in Figure 4. Initially, a stable background signal f0 is obtained in the presence of buffer. Then, sample solution containing the free partner B is injected - association phase. The biomolecules with free binding sites interact with the immobilized ligand A. A decrease of frequency is observed as surface mass on the crystal increases and the equilibrium change feq is eventually achieved. Then, buffer is injected again and the dissociation of immunocomplexes is observed. From this phase, the dissociation constant kd can be obtained independently, fa represents the amount of the surface-bound immunocomplex at the beginning of the dissociation:

 

 

3.2 Example of characterization of anti-secaline antibody

For a better understanding, the described model will be demonstrated on measurement of kinetic parameters for the monoclonal antibody prepared against the rye proteins secalins (kindly provided by the Institute of Food Research, Norwich, UK). The sensing surface of piezoelectric quartz crystal (10 MHz) was silanized using g-aminopropyltriethoxysilane and thus obtained amino group was used for covalent attachment of secalins using glutaraldehyde. Samples containing different concentrations of the studied antibody were allowed to interact with secaline-modified sensors placed in the flow-through cell, both association and dissociation phases were recorded and thus obtained signal traces are shown in Figure 5.

 

 

For each individual curve, the values of kobs and feq were determined using non-linear curve fitting of the frequency f vs. time t traces to equation (3). From the dissociation part of the traces, the values of kd were directly determined using non-linear regression and equation (4). Plot of kobs values against the corresponding concentrations c of antibodies should provide linear dependence; substitution in equation ((3), kobs = ka c + kd, where ka is equal to the slope and kd to the intercept. The kobs vs. c plot as well as the obtained values of constants are shown in Figure 6.

 

 

The determined values of constants should always be critically interpreted as these need not exactly agree with the corresponding values obtained from experiments with both affinity partners free in solution. The immobilization of small molecules (e.g. haptens) can affect the conformation. In addition, the density of ligands and mass transport effects should be considered, too. The ligand can be immobilized either as a single monolayer or inside a three-dimensional dextran matrix. The latter approach provides higher levels of signal; on the other hand, dissociation can be disturbed by "rebinding" effects.

 

4. Immobilization of Biomolecules on Piezosensors

The immobilization procedures suitable for piezosensors include quite different strategies ranging from simple physical adsorption to covalent binding on activating sublayers and finally even oriented attachment of biomolecules. The generally applicable immobilization procedures10 are suitable also for piezosensors; some published approaches11 will be mentioned in detail.

Before any covalent immobilization procedures, the inert surface of metal (mostly gold) electrodes deposited on the quartz plate of the sensor should be activated. In this way, silanization was widely used for activation,12 the suitable reagents include g-aminopropyltriethoxysilane (APTS) providing amino groups and g-glycidoxypropyltrimethoxypropane (GOPS) providing reactive oxirane groups. Thus modified surfaces are suitable either directly (GOPS) or after glutaraldehyde activation (APTS) for attachment of biomolecules with free amino groups (e.g. lysine residues from proteins).

Another popular approach employs thiocompounds (thiols and disulfides) irreversibly adsorbing on gold surfaces. Thus formed self-assembled monolayers13 are quite convenient for further immobilization steps. Thus, cystamine/cysteamine and paminothiophenol provide surface amino groups; thiopropionic acid, mercaptoundecanoic acid and lipoic acid result in available carboxyl groups.14 Special reagents as 3,3'dithiodipropionic acid di(N-hydroxysuccinimide ester) (DTSP) attach on the surface and provide reactive NHS-groups suitable for immediate coupling of biomolecules with free amino groups.

From proteins, self-assembled layers of avidin (streptavidin)15 and protein A (G) appear convenient for further immobilization steps. The self-assembling feature can be even implemented in recombinant proteins. Thus, self-assembled glutathione layer was further extended by binding of a chimerical protein consisting of calmodulin and glutathione-S-transferase.16 Spontaneous binding of protein A to gold was further enhanced by genetically inserting cysteine residue at the C-end of the molecule.17

Thus obtained surfaces can be directly used for immobilization of bioligands in the form of a monolayer. Sometimes, hydrophilic layers (e.g. dextrans activated by periodate oxidation) are used to provide a thicker three-dimensional matrix with much higher ligand coupling capacities.18 Similarly, the alternating protein polyelectrolyte multilayers were shown to provide increased response; poly(styrenesulfonate) was used together with immunoglobulin.19

Antibodies are probably the most often immobilized protein molecules. The oriented immobilization through protein A forming complex in the Fc part of antibody molecule remains the most convenient method.20 The protein A-antibody complex can be stabilized using dimethylpimelimidate in order to facilitate regeneration of the biosensing layer. Alternatively, surface oligosaccharides from the Fc part might be oxidized using periodate and thus activated antibody will be attached to surface with available amino groups. Immobilization of antibodies using adsorbed Langmuir-Blodgett method21 does not seem to provide robust sensing interfaces. Linker lipids in a phosphatidylcholine monolayer were considered for oriented Fab' fragments immobilization.22

For oligonucleotide probes, the easiest way is to attach biotin at the end of the nucleic acid strand and form a very stable complex with avidin (streptavidin) covalently bound to the piezosensor surface. Low-cost silver-based piezosensors were protected with polystyrene and photobiotin was bound by UV-irradiation followed by avidin layer deposition.23 Alternatively, thiol-tagged oligonucleotides can be self-assembled on the sensing surface.24 However, for peptide nucleic acids (PNA) as probes, immobilization through the streptavidin attached on a biotinylated phospholipid bilayer was much more efficient compared to self-assembled thiolated probes.25 Electropolymerizable pyrrole-oligonucleotides were reported as suitable approach for hybridization sensors.26 An interesting dendritic nucleic acid probes were tested with piezosensors.27 Electrostatically bound multilayers were also investigated.28

For recombinant proteins containing oligohistidine tag, the metal affinity complexation with nickel ions and surface-attached nitrilotriacetic acid as a ligand is promising way for a reversible immobilization.

 

5. Applications

The piezoelectric biosensors were successfully used for assays of many different analytes as already reviewed in the literature.29 The working formats include direct, competitive and displacement assays. Large analytes (proteins, viruses and bacteria) can be measured directly after binding to the immobilized bioreceptor (e.g. antibody). An improved response is obtained through sandwich formation with suitable antibodies, which might be even attached to heavy tags functioning as enhancers of the measured signal. An overview of selected bioanalytical assays based on piezoelectric sensors is summarized in Table 1.

 

 

5.1 Detection of viruses, bacteria and other cells

Piezoelectric immunosensors suitable for detection of viruses and bacteria associated with acute diarrhea30 demonstrated detection limits of 106 microorganisms per mL. Herpes simplex viruses type 1 and 2, varicella-zoster virus, Epstein-Barr virus and cytomegalovirus were all detected from 104 viruses per mL using direct assay.31 In the case of detection of hepatitis A and B viruses,32 detection limits were 105 per mL. Sensor based on the DNA-RNA hybridization was employed for the detection of two plant orchid viruses, Cymbidium mosaic virus (CymMV) and Odontoglossum ringspot virus (ORSV). Specific oligonucleotide probes modified with a mercaptohexyl group at the 5'-phosphate end were directly immobilized and exposed to test solutions containing viral RNA; 10 ng mL-1 of viral particles was successfully detected in crude plant extracts.33

A direct assay format was reported for Chlamydia trachomatis.34 The anti-surface lipopolysacharide Ab was attached to the sensor through anti-mouse F(ab')2 fragment immobilized to cystamine monolayer using sulfosuccinylimidyl 4-(p-maleimidophenyl)butyrate; 260 ng mL-1 was detected in urine. Detection of Pseudomonas aeruginosa in milk and diary samples employed displacement format,35 which allowed to detect36 105 cells per mL. Staphylococcus aureus was detected from 5x105 cells per mL. Piezoelectric immunosensor for Listeria monocytogenes was reported.37 A direct sensor for Salmonella enteritidis38 was made by immobilizing antibodies on the divinylsulphone activated poly-m-aminophenol film; sensor was able to detect 105 cells per mL within 25 min.

Convenient approaches employed latex agglutination in the presence of microorganisms, generating large viscoelastic changes on the sensing surface. Such format was reported for Treponema pallidum in serum.39 Electropolymerized layer for antibody attachment was reported for Salmonella paratyphi detection40 at 105 cells mL-1. Styrene-butadiene-styrene block copolymer was selected as a new membrane material to immobilize antibody used for detection of Mycobacterium tuberculosis.41 Plasma-polymerized ethylenediamine film was employed for immobilization of antibodies against Alexandrium affine, the red tide causing plankton.42

Several sensors were designed for blood cells. Human erythrocytes were measured using anti-glycophorin A antibody attached to the piezosensor with the limit of detection at 1000 cells per mL in whole blood.43 Similar sensors employed anti CD 3 gp Mr 19-29 kDa antibody for detection of human T-lymphocytes starting from 5000 cells/mL of blood,44 and anti CD 18 antibody for granulocytes.45

5.2 Determination of proteins

A large group of papers is dealing with detection of antibodies present in serum after exposure to toxic agents. Thus, anti human immunodeficiency virus antibodies were detected in serum with selectivity comparable to ELISA.46 Similarly, respiratory syndrome virus infection47 and African swine fever disease48 were identified in porcine sera and Salmonella enteritidis in chicken meat and egg.49 Detection of antibodies against the African swine fever virus was realized using the recombinant viral attachment protein p12; an enhanced response was achieved using peroxidase label and 4-chloro-1-naphthol as substrate leading to insoluble product precipitating at the sensing surface.50 Exposure to Schistosoma japonium was detected in rabbit serum.51 Assay of the staphylococcal enterotoxin C-2 compared different antibody immobilization approaches,52 the enterotoxin B53 (SEB) was detected, too.

The latex agglutination immunoassay54 was used for detection of rheumatoid factor,55 human IgM,56 and C-reactive protein.57 Staphylococcus epidermidis agglutination with the corresponding antibody served for identification of sera infected with the microorganism.58 Poly(ethylene glycol)-enhanced agglutination assay of transferrin in human serum59 provided limit of detection at 2 nmol L-1.

Piezoelectric immunosensors (for review see60) were able to detect directly a wide range of clinically important analytes: complement inhibitors C161 and C6,62 factor B,63 hepatitis B surface antigen,64 a-fetoprotein65 and ferritin66 in serum, immunoglobulin E (5 – 300 IU mL-1).67 Sandwich assay for insulin68 exhibited limit of detection at 1 ng mL-1.

5.3 Assays of nucleic acids

Piezosensors were applied for the monitoring of hybridization quite early,69 for reviews see works of Mickelsen and Tombelli et al.70 Recently, a novel concept of a gene sensor employed primer extension after hybridization with template resulting in a real-time measurement format.71 Hybridization of the biotinylated 25-mer oligonucleotide probe (linked to neutravidin-modified piezosensor) with complementary, non-complementary and single-base mutated 25-mer targets were monitored at ambient and elevated temperature, the latter allowing detection of single-base mutations.72

It is convenient to amplify the target DNA sequence using the polymerase chain reaction prior to the piezosensor measurement. In this way, genotypes of human apolipoprotein E were determined.73 Few Escherichia coli bacteria can be detected in 100 mL of water,74 detection of Aeromonas hydrophila was also reported.75

Various signal amplification strategies were developed to improve sensitivity of detection. Anti-dsDNA antibodies were reported to amplify signal during detection of the Tay-Sachs disease.24 Binding of biotinylated liposomes,76 deposition of gold nanoparticles77 and even intercalation of actinomycine D bound to magnetic nanoparticles78 were another ways for signal increase. Recently, piezoelectric biosensors were considered as a convenient tool for detection of genetically modified organisms (GMO).79

5.4 Measurement of small molecules

For small molecules as pesticides, drugs and hormones, the competitive measurement is needed in order to achieve measurable changes of frequency; the analyte is mixed with antibody to form immunocomplexes and the remaining free binding sites of the antibody can subsequently interact with sensing surface modified with a derivative of the analyte.

Cortisol immunoassay80 exhibited limit of detection at 100 nmol L-1, cocaine was detected at 33 nmol L-1 using the anti-benzoylecgonine antibody.81 For b-estradiol, the specific receptor was immobilized on the surface82 providing detection limit of 8 nmol L-1. A cholestyramine resin-coated piezosensors were used for convenient detection of bile acids (e.g. taurodeoxycholate at 0.2 mmol L-1), the sensing surface allowed for several hundredths of regenerations.83 b-Indolyl acetic acid was measured competitively84 from 3 nmol L-1. The aminophenylboronic acid-modified surface served for measurement of fructose85 starting from 0.5 mmol L-1.

The early described immunosensor for the herbicide atrazine employing the drying approach86 stimulated other works describing assay of atrazine87 and other pesticides88 in flow-through formats. Assay for polyaromatic hydrocarbons was designed using anti benzo[a]pyrene-specific antibody and competitive format.89 For non-polar analytes, the presence of organic solvents can improve performance of piezoelectric immunosensors.90 For this purpose, the artificial molecularly imprinted polymers (MIP) represent robust and stable recognition elements, as demonstrated by the detection of terpenes.91 Molecular recognition sites for the nucleotides adenosine 5'- monophosphate, guanosine 5'-monophosphate, cytosine 5'- monophosphate and uridine 5'-monophosphate were imprinted in an acrylamide-aminophenylboronic acid copolymer on the piezosensor surface providing novel way for sequencing of nucleic acids.92 The reported MIP layers on piezosensors were also applied for measurement of paracetamol93 and aminoantipyrine.94

Recently several papers described detection of small molecules using direct assay format;95 in fact, the measured frequency changes do not correspond to the theoretically bound material (missing mass effect). Instead, the immobilized bioligands undergo conformation change upon complex formation with the small analyte. This results in viscoelastic changes of the surface-attached layers and this process is measured by the piezosensor with a high sensitivity.

5.5 Affinity studies using piezoelectric sensors

The technique demonstrated above was used to characterize several monoclonal96 as well as recombinant97 antibodies. Validation of results obtained using piezosensors and resonant mirror-based sensors were reported.98 In the case of polyclonal antibodies, exact kinetic parameters are not obtained; nevertheless, the quantitative comparison of different antibody products is possible. Moreover, fast determination of affinities is useful during screening and development of antibodies. The kinetic parameters enable to select the most suitable antibody for the planned use when several monoclonals are available. Of course, qualitative results from affinity studies are very important, too.

A detailed kinetic characterization of berberine interaction with albumin99 provided moderate affinity described by Ka of 4x104 L mol-1. The affinity of the maltose binding protein-nitro reductase fusion (MBP-NR) to electropolymerized films of N-(3-pyrrol-l- ylpropyl)-4,4'-bipyridinium (PPB) has been studied.100 Permeability of bacterial cell walls was studied using interaction of peptides representing different regions of human bactericidal/permeability-increasing protein (BPI) and polymyxin B (PmB) with lipopolysaccharide attached to the piezosensor.101 Piezosensors were conveniently used for kinetic characterization of isolates from combinatorial phagemid libraries. Kinetic rate constants for interaction between human pancreatic secretory trypsin inhibitor and Legionella pneumophila were determined in this way.102

For epitope mapping, variability of amino acids in the binding site region of structural protein VP1 of parvovirus B19 was monitored using piezosensors providing the corresponding equilibrium dissociation constants.103 Systematic ligand-binding studies of the interaction between steroids (17 testosterone and b-estradiol derivatives) and anti-steroid antibodies were performed to assess the effect of biotinylation of the steroid molecule in different positions.104

Living endothelial cells were cultured on the piezosensor surface as the biological signal transduction element. Nocodazole, a known microtubule binding drug, alters the cytosceletal properties of living cells resulting in cell contact breaks measured as frequency changes.105

Kinetics of DNA hybridization was monitored using the network analysis measuring method.106 Studies of metal complex binding to nucleic acid employed DNA layers attached electrostatically to methylated 4-thiopyridine layers self-assembled on the gold surface;107 Ka was equal to 2.0x104 L mol-1 for hexaammineruthenium(III) complex.108 Similar study was carried out also for the tris(2,2'- bipyridyl)cobalt(III) complex. DNA oxidative damage induced by the vitamin C-Fe (III) system represented another convenient application.109 Interactions of drugs (nogalamycin110 and platinum-based anticancer111) with DNA open another important area.

Piezosensor was used to measure the equilibrium constant and binding kinetics of a PCN antigen to double-stranded DNA model fragments. The antigen, which is negatively charged on its outer side and positively on its inside, was shown to slip along the DNA fragment.112 Similar study was carried out for histone F3 binding.113 A sequence-specific binding of bZIP 56-mer peptides (having both the basic region for binding and the leucine zipper region for dimerization) to the DNA strand on the 27 MHz piezosensor was observed and kinetically characterized.114

Interaction of nucleic acids with cleaving enzymes115 can be followed by piezosensors with high sensitivity. In this way, multilayers composed of bis-biotinylated dsDNA and streptavidin were shown to be bioactive as evidenced by cleavage with restriction endonuclease.116 However, prolongation of DNA chain using polymerase can be monitored, too.117

Specific binding of lectin - peanut agglutinin to ganglioside G(M1)-doped solid supported lipid bilayers118 exhibited Ka of 8.3x105 L mol-1. Monosialoganglioside monolayers were applied for binding of wheat germ agglutinin.119 Adsorption behavior of artificial glycoconjugate polymers with different saccharide chain lengths and with different backbone structures120 was investigated as a model saccharide-based biorecognition element.

Stable solid-supported bilayers consisting of an octanethiol monolayer chemisorbed on gold and a physisorbed phospholipid monolayer varying in its lipid composition as the outermost layer were used for studies with the Raf-Ras binding domain and the cysteine-rich domain Raf-C1 fused to maltose binding protein, implying mostly electrostatic character of interaction.121

The degradation of polymeric layers coated on surface of piezosensors represents convenient route for detection of the corresponding hydrolases; polyhydroxybutyrate depolymerase was able to degrade poly(ester) films.122

 

6. Conclusions

For a long time, piezoelectric systems were rather neglected by the biosensor community; the common feeling was that these devices are not sensitive enough compared to other types of transducers. As theoretical backgrounds for operation of piezosensors in liquids become completed and many successful applications were reported in the literature, this situation is changing gradually. This change of attitude was fully evident at the first conference dedicated to piezoelectric and acoustic sensors – QCM 2002 in Brighton, UK.

The aim of this review was to demonstrate different types of analytes measurable with piezosensors using bioanalytical assay formats. The piezoelectric biosensors represent a convenient tool for fast and simple determination of viruses, bacterial and other cells, proteins, nucleic acids and small molecules as drugs, hormones and pesticides. Usually, no additional unstable reagents are required for the analysis. In addition to the traditionally interpreted mass changes, additional sensing approaches appeared. The presence of analyzed molecules initiates agglutination reactions and conformation changes of attached biolayers. The associated viscoelastic effects provide novel ways to improved sensitivity of measurements.

Piezosensors are also useful as a research tool for biochemistry and biology. The direct label-free and real-time monitoring of affinity interactions with piezosensors represents an economic alternative to the overpriced optical systems based on the surface plasmon resonance phenomenon. The theoretical background for determination of kinetic rate and equilibrium constants was presented here and the approach was demonstrated on the model interaction of antibody with the corresponding antigen (protein secalin). In this way, valuable characteristics of affinity binding reaction can be obtained easily and quickly.123-131

 

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Received: December 26, 2002
Published on the web: July 21, 2003

 

 

* e-mail: skladal@chemi.muni.cz