versão impressa ISSN 0103-5053
J. Braz. Chem. Soc. v.14 n.6 São Paulo nov./dez. 2003
Catarina dos SantosI; Julio Zukerman-SchpectorII; Paulo M. Imamura*, I
IInstituto de Química, Universidade Estadual de Campinas, CP 6154, 13084-971 Campinas - SP, Brazil
IIDepartamento de Química, Universidade Federal de São Carlos, CP 676, 13565-905 São Carlos - SP, Brazil
Chemical transformations of the C-ring abietic acid (1a) for the preparation of some new chiral synthons are described. From the intermediate keto-aldehyde 9, readily available from 1b through the ozonide 2, the new tetracyclic diterpene 4 was synthesized. Additionally, from 13 and 17, previously prepared from 2, the bicyclic compounds 14, 15 and 16 and the derivatives containing the abeo-abietane[6,6,5] framework 19 and 20 were also synthesized.
Keywords: abietic acid, ozonization, chiral synthon, abeo-abietane derivatives
Neste trabalho descrevemos as reações de transformações químicas do anel C do ácido abiético (1a) com o objetivo de gerar novos síntons quirais. A partir do intermediário ceto-aldeído 9, obtido através da reação de ozonólise do abietato de metila (1b), foram sintetizados derivados bicíclicos tais como 14, 15 e 16 e um novo diterpeno tetracíclico 4. Foram também sintetizados, a partir 17, novos derivados contendo esqueleto abeo-abietano[6,6,5] tais como 19 e 20.
Abietic acid (Figure 1, 1a) is a diterpene readily available from an oleoresin produced by Pinus elliottii1 that shows activity as a chemical defense against insect and pathogenic fungi and bacteria.2 This acid has been used widely as an enantiomerically pure starting material in organic synthesis.3 Examples are the syntheses of Ambrox®, warburganal4 and some other sesquiterpenes.5 In order to evaluate the biological activities of the compounds obtained by modifying the framework of methyl ester 1b, we first synthesized the stable ozonide 2 that showed activity against some lines of non-small lung cell, melanoma and breast cancers.6 We have also shown that some Diels-Alder adducts of 1b presented activities against Staphylococcus aureus, Bacillus subtilis and Micrococcus luteus.7 Starting with 2 we recently completed the synthesis of 3,8 an analogue of a new class of compound named oidiolactone.9 In this paper we describe the transformation of 9 into the new tetracyclic derivative 4 and into its known diastereoisomer 5,3 the transformation of 13 into the bicyclic compounds 14, 15 and 16 and also transformation of 17 into the new derivatives containing abeo-abietane[6,6,5] framework 19 and 20. Interestingly, compound 9, 13 and 17 were all previously synthesized from ozonide 2.6,8
Results and Discussion
To explore a different reactivity of the double bonds in the B and C-rings of 1b, preliminary semi-empirical calculations (AM1 and PM3) were carried out using the SPARTAN®10 and Hyperchem® programs.11 The first analysis made was the Mulliken charges (Figure 2 - A and B) which showed the C-ring to have a small, but higher, coefficient than the B-ring. On the other hand, HOMO coefficient calculations showed a slightly higher coefficient for C-7 than C-13 (Figure 2 - C).
These results led us to conclude that an electrophilic attack can occur either on the double bond at C-7 or at C-13, and that the regioselectivity would depend on the reaction conditions and reactivity of the reagents. The literature gives examples where treatment of 1b with OsO44 or an epoxidation reaction with mCPBA12 occurred preferentially on the C-ring. The oxidation of 1b with I2 /KHCO3 at 50 oC (THF:H2O; 47:1) also occurred on the C-ring and furnished the b-epoxide 6. However, when the reaction was carried out at 30 oC, the C-7 oxo-derivative 713 (Figure 3) was obtained as the main product, showing that the reaction had occurred on the B-ring.
In a previous study we reported that the ozonolysis reaction of 1b in CH2Cl2 furnished compound 2, showing that the reaction took place at the C-7 and C-13 double bonds.6 Carrying out the reaction using MeOH-CH2Cl2 (1:1) as solvent, we obtained compound 8 in 28% yield (Figure 3), in addition to a highly polar polymerized material. The formation of compound 8 showed that, under this condition, the electrophilic attack occurred at C-7 and C-13 double bond of 1b.
Compound 8 was characterized by 1H NMR spectral analysis where the signal at d 4.08 (s, 1H) was assigned to H-14 and the signals at d 3.39, 3.43 and 3.64 were attributed to the methoxy groups of the acetal moiety and the methyl ester, respectively. The presence of an epoxy group at C-7 was observed in the 1H NMR spectrum, through the signal of H-7 at d 3.21, and was confirmed by 13C NMR spectrum which showed signals of C-7 and C-8 at d 54.6 (CH) and at d 59.8 (C), respectively. The chemical shifts at d 56.2 and at d 57.1 confirmed the presence of the two methoxy groups of the acetal moiety at C-14 and the carbomethoxy group was observed at d 52.0. Two carbonyl carbons, one at d 178.5 assigned to the ester (C-18), and another at d 214.6, assigned to the ketone (C-13) were also observed. All other signals were in agreement with those observed for abietane models.14
In an attempt to cleave the side chain of the bicyclic intermediate 9,6 we submitted it to a Baeyer-Villiger reaction induced by mCPBA. Two compounds were isolated from the crude reaction product and were spectroscopically characterized as the tetracyclic compounds 4 (50%) and 5 (33%), instead of the expected product 10 (Scheme 1).
The 1H NMR spectrum of 4 showed a triplet at d 3.93 (J 2.8 Hz) assigned to H-7, two multiplets at d 1.79 and d 1.88 assigned to H-12 and two singlets at d 1.10 and d 1.22 assigned, respectively, to the methyl groups at C-20 and C-19. The 13C NMR spectrum showed chemical shifts at d 16.1 and at d 16.2, which were assigned to the methyl groups at C-16 and C-17 and the signal at d 114.1 was assigned to the C-13 of the ketal group. The chemical shift at d 177.2 was assigned to the carbonyl carbon of the lactone (C-14) and the presence of a carbonyl group was confirmed through the IR absorption at 1768 cm-1. All spectroscopic data were in good agreement with those reported for the model ambraketal analogues 11 and 12, previously synthesized by Scheidegger et al.15 (Figure 4) as well as for abietane model compounds.3,8 Compound 5 was also characterized by spectroscopic data analysis and through comparison with those reported previously by Haslinger3 (Figure 4).
In view of these results we decided to study an alternative sequence, starting with 13,6 to cleave its side chain. Our first choice was to analyze the preparation of intermediate 15 (Scheme 2).
The reduction of 13 with NaBH4/MeOH furnished, as expected, an inseparable mixture of epimeric alcohols 14 in 80% yield. The 1H NMR spectrum of this mixture showed a multiplet at d 3.34, which was assigned to H-13, and the 13C NMR spectrum the signal at d 76.6 was also attributed to C-13. Following the sequence, the dehydration reaction of 14 was carried out using POCl316 where a mixture of olefins 15/16 (88:12) was obtained in 33% yield. The best result was obtained when alcohol 14 was reacted with MsCl, followed by treatment with DBU-Bz17 to furnish a mixture of 15/16 (88:12) in 54% yield.
Compound 15 was characterized by 1H NMR spectral analysis where a chemical shift of H-13 as a broad triplet at d 5.04 (J 7.2 Hz) and methyl groups of H-16 and H-17 attached to sp2 carbons at d 1.60 and at d 1.69 were observed. The 13C NMR spectrum showed the signal of C-15 at d 132.2 (c) and the signal of C-13 at d 125.2 (cH) confirming the presence of the double bond. For compound 16, the chemical shift of H-12 was observed at d 5.25 (ddd, J 15.8; 7.2 and 4.0 Hz) and the chemical shift at d 5.41 (dd, J 15.8 and 6.0 Hz) was assigned to H-13. The chemical shift of H-15 appeared at d 2.22 as a double septet and H-16 and H-17 appeared as a doublet at d 0.94. The presence of the double bond was also confirmed through 13C NMR spectral analysis where the chemical shift of C-12 was observed at d 123.7 (cH) and the chemical shift of C-13 was observed at d 139.8.
Since 13 is a highly oxygenated compound containing a very reactive oxirane ring and knowing that the transformation of the epoxide to the corresponding olefin is feasible, compound 13 was submitted to reaction with thiourea oxide18 and PPh3/I2.19 In both cases only the starting material was recovered and attempts to open the epoxide to the corresponding diol using different conditions, such as 85% KOH/DMSO,20 H2SO4/acetone21 or KOH/H2O also failed. Further investigations on the bicyclic system to obtain potentially biologically active compounds such as anticancer, bactericidal and fungicidal compounds are now underway.
In order to obtain a more advanced chiral intermediate than the tricyclic compound 17, which was previously prepared in a reasonable yield (45%, 2 steps)8 from 2, an oxidation reaction of the hydroxyl group at C-17 was considered. Unfortunately, the oxidation reaction using different reagents, such as calcium hypochloride,22 Moffatt oxidation23 and Jones reagent,23 led only to an intractable mixture of products or recovery of the starting material.
Surprisingly, treatment of 17 with PCC24 led to the triol 19 in 30% yield instead of to the expected oxidized product 18. This triol was characterized through 1H NMR spectral analysis, where a signal at d 4.35 (dd, J 12.0 Hz and J 6.0 Hz) assigned for H-7, clearly deshielded when compared with H-7 of 17 (s, d 3.27), was observed, consistent with equatorial orientation of the hydroxyl group. All other signals, including H-12 at d 3.15 (ddd, J 13.5; 7.5 and 5.2 Hz), were in agreement with the structure depicted as 19. The HRMS data (M+. at m/z 382.23553) also confirmed the molecular formula C23H34O6. The 13C NMR spectrum showed the chemical shifts of C-7 and C-8 at d 70.0 (CH) and 81.0 (C), respectively, and was consistent with the structure proposed as 19. The IR spectrum also showed three absorptions of hydroxyl group at 3546, 3515 and 3549 cm-1. Conversion of the epoxide to the diol in high selectivity, using chromium complexes like [Cr(N-tBu)Cl3(dme)] or [V(Ntol)Cl3] (dme = 1,2-dimethoxyethane, tol = p-tolyl) is known in the literature and, according to Leung et al.,25 formation of a five membered ring chelate seems to be more favorable than the six membered ring.25 Probably the chelation of the chromium ion with the oxygens of the epoxide and the hydroxyl group at C-12 from the a face, led to the attack of water from the b face (at C-7), to furnish the trans diol, as shown in Scheme 4.
Due to the difficulties found in oxidizing compound 17, a Baeyer-Villiger reaction followed by oxidation and decarboxylation, in order to obtain 21, was considered. Following this sequence, treatment of 17 with mCPBA furnished 20 in 80% yield (Scheme 5).
The 1H NMR spectrum of 20 showed the chemical shift of methyl groups at 1.26 (d, J 6.2 Hz, 3H, H-16) and 1.28 (d, J 6.2 Hz, 3H, H-17), slightly deshielded in comparison with those of 17 (d 1.13; d, J 6.2 Hz), as well as H-15 which now appeared at d 5.08 (sept, J 8 Hz). The 13C NMR spectrum of 20 showed the chemical shift of methyl groups at d 21.8 (C-16) and d 21.9 (C-17) and a carbinolic carbon C-15 at d 68.2. The IR absorption at 1725 cm-1 and the 13C NMR chemical shift at d 173.4 (C-13) confirm the presence of a carbonyl ester and the success of Baeyer-Villiger oxidation reaction. Unfortunately, the oxidation reaction of the secondary alcohol of 20 using PCC or Jones reagent led only to recovery of the starting material.
None of the synthesized compounds presented significant activity against Artemia salina and any activities against S. aureus, B. subtilis and M. luteus. Further work is in progress in order to obtain a more elaborated tricyclic chiral synthon for the synthesis of drimane sesquiterpenes and biologically active compounds.
NMR spectra were recorded on a Gemini 300PVarian Instruments and Bruker AC (1H NMR at 300 MHz and 13C NMR at 75 MHz) or on a INOVA 500 Varian Instruments (1H NMR at 500 MHz and 13C NMR at 125 MHz), in CDCl3 as solvent with TMS as internal standard. IR spectra were measured on a Perkin Elmer 1600 or on a BOMEM MB-100 FtIR. Optical rotation was measured on a Carl Zeiss photoelectric polarimeter. High-resolution mass spectra (HRMS) were recorded with a VG 7070E spectrometer. For the HOMO/LUMO and Mulliken charge calculations, the programs PC SPARTAN® plus (version 1.5) and Hyperchem 6.03 professional® were used. Column chromatography was performed by using Merck silica gel 60 (70-230 mesh) and TLC plates were performed using Merck 60F254 silica gel on glass-supported plates.
Synthesis of 8 from 1b
A stream of ozone was passed through a solution of 1b (238.0 mg, 0.78 mmol) in anhydrous CH2Cl2/MeOH (1:1) (26.0 mL) at -78 °C until a blue color persisted. Excess ozone was removed by passing nitrogen through the solution and the reaction mixture was treated with PPh3 (376.0 mg, 1.4 mmol). After stirring the mixture at room temperature for 8 h, the solvent was removed in a rotary evaporator and the residue was chromatographed on silica gel (n-hexane-EtOAc, 85:15) to give 8 (87,8 mg, 28%) as a colorless oil: [a]D20 +24.0 (c 1.0, CHCl3); IR (film) nmax/cm-1: 2936, 1725, 1467, 1387, 1247, 1083, 755; 1H NMR (300 MHz) d: 0.80 (s, 3H, H-20), 0.98 (m, 1H, H-1), 1.12 (d, J 7.0 Hz, 6H, H-16, H-17), 1.19 (s, 3H, H-19), 1.38 (m, 1H, H-9), 1.50 (m, 2H, H-2), 1.53 (m, 2H, H-3), 1.65 (m, 1H, H-11), 1.72 (m, 2H, H-6), 1.80 (m, 1H, H-5), 1.82 (m, 1H, H-11'), 1.84 (m, 1H, H-1'), 2,51 (ddd, J 16.3, 10.0, 7.4 Hz, 1H, H-12), 2.62 (sept, J 7.0 Hz, 1H, H-15), 2.76 (ddd, J 16.3, 10.0, 4.6 Hz, 1H, H-12' ), 3.22 (s, 1H, H-7), 3.39 (s, 3H, H-21), 3.43 (s, 3H, H-22), 3.64 (s, 3H, H-23), 4.08 (s, 1H, H-14); 13C NMR (75 MHz) d: 14.3 (C-20), 17.1 (C-19), 17.4 (C-11), 18.1 (C-2), 18.2 (C-16), 18.3 (C-17), 24.0 (C-6), 35.6 (C-10), 36.9 (C-3), 37.7 (C-1), 40.8 (C-15), 41.0 (C-5), 42.6 (C-12,) 46.6 (C-4), 52.0 (C-23), 54.6 (C-9), 54.6 (C-7), 56.2 (C-21), 57.1 (C -22), 59.8 (C-8), 106.7 (C-14), 178.5 (C-18), 214.6 (C-13); HRMS: Calcd for C23H38O6: 410.26684; Found: 410.26295 (M+)
Syntheses of 4 and 5 from 9
To a solution of 9 (50.0 mg, 0.27 mmol) in anhydrous CH2Cl2 (5.0 mL) was added 99% mCPBA (48.0 mg, 0.54 mmol) and NaHCO3 (12.0 mg, 0.27 mmol). The reaction mixture was kept in the dark with stirring at room temperature for 15 days. The mixture was filtered and the organic layer was washed with saturated NaHCO3 solution. The organic layer was dried over anhydrous MgSO4 and the solvent removed in a rotary evaporator. The residue was chromatographed on silica gel (n-hexane- EtOAc, 85:15) to furnish two products. Compound 4 (25.5 mg, 50%) was obtained as a colorless oil; [a]D20 -11.5 (c 1.5, CHCl3); IR (film) nmax/cm-1: 3511, 2975, 1768, 1725, 1389, 1250, 1162, 1072, 737; 1H NMR (300 MHz) d: 0.94 (m, 1H, H-1), 1.00 (d, J 7.0 Hz, 3H, H-16), 1.03 (d, J 7.0 Hz, 3H, H-17), 1.10 (s, 3H, H-20), 1.22 (s, 3H, H-19), 1.32 (ddd, J 14.4, 3.0, 2.2 Hz, 1H, H-6), 1.58 (m, 1H, H-3), 1.62 (m, 2H, H-2), 1.76 (m, 1H, H-6'), 1.78 (m, 1H, H-9), 1.79 (m, 1H, H-12), 1.80 (m, 1H, H-1'), 1.82 (m, 1H, H-3'), 1.88 (m, 1H, H-12'), 1.92 (m, 2H, H-11), 2.04 (sept, J 6.8 Hz, 1H, H-15), 2.52 (dd, J 13.0, 1.8 Hz, 1H, H-5), 3.70 (s, 3h, h-21), 3.93 (t, j 2.8 Hz, 1H, H-7), 4.07 (d, J 2.2 Hz, OH); 13C NMR (75 MHz) d: 16.1 (C-16), 16.2 (C-17), 16.5 (C-20), 16.6 (C-11), 16.9 (C-19), 17.6 (C-2), 25.8 (C-12), 27.5 (C-6), 34.5 (C-15), 36.6 (C-3), 38.5 (C-1), 38.5 (C-10), 40.1 (C-5), 41.0 (C-9), 47.1 (C-4), 52.2 (C-21), 68.5 (C-7), 80.7 (C-8), 114.7 (C-13), 177.2 (C-17), 178.9 (C-14); HRMS: Calcd for C21H32O6: 380.21989; Found: 380.21858 (M+). Compound 5 (17.0 mg, 33%) was obtained as colorless oil. All spectroscopic data were in a good agreement with those reported in the literature.3
Synthesis of 14 from 13
To a solution of 13 (79.3 mg, 0.22 mmol) in anhydrous MeOH was added NaBH4 (10.0 mg, 0.26 mmol). The mixture was stirred in an ice bath for 1 h, then the solvent was evaporated and the residue was dissolved in EtOAc and washed with a saturated NaCl solution. The organic layer was dried over anhydrous MgSO4 and the solvent was removed in a rotary evaporator. The residue was chromatographed on silica gel (n-hexane-EtOAc, 85:15) to give a mixture of epimeric alcohol 14 (60.8 mg, 76%) as colorless oil. IR (film) nmax/cm-1: 3540, 2954, 2872, 1728, 1436, 1389, 1195, 737; 1H NMR (300 MHz) d: 0.88 (d, J 6.7 Hz, 3h, H-16), 0.91 (d, J 6.7 Hz, 3h, H-17), 0.94 (s, 3H, H-20), 1.04 (m, 1H, H-1), 1.22 (s, 3H, H-19), 1.29 (m, 1H, H-11), 1.38 (m, 1H, H-9), 1.49 (m, 1H, H-14), 1.54 (m, 2H, H-2), 1.57 (m, 1H, H-3), 1.61 (m, 2H, H-12), 1.64 (m, 1H, H-11'), 1.66 (m, 2H, H-6), 1.78 (m, 1H, H-3'), 1.82 (m, 1H, H-1'), 1.86 (m, 2H, H-5, -OH), 3.20 (t, J 2.6 Hz, 1H, H-7), 3.34 (m, 1H, H-13), 3.66 (s, 3H, H-21), 3.74 (s, 3H, H-22); 13C NMR (75 MHz) d: 14.7 and 14.8 (C-20), 17.0 (C-19), 17.2 and 17.4 (C-16), 18.1 (C-2), 18.6 (C-17), 20.4 and 20.7 (C-11), 23.7 (C-6), 33.2 and 32.8 (C-12), 33.6 and 33.7 (C-15), 34.6 and 34.7 (C-10), 36.8 (C-3), 37.6 (C-1), 41.2 (C-5), 46.5 (C-4), 52.1 (C-21), 52.3 (C-22), 53.8 (C-9), 57.2 (C-7), 58.9 (C-8), 76.6 (C-13), 170.7 and 170.9 (C-14), 178.4 (C-18); HRMS: Calcd for C22H36O6 _ H2O: 378.24062; Found: 378.24059 (M+ _H2O).
Syntheses of 15 and 16 from 14
1st method. To a solution of 14 (165.7 mg, 0.42 mmol) in anhydrous CH2Cl2 (0.5 mL), at 0 oC, anhydrous pyridine (40 mL, 0.96 mmol) and mesyl chloride (75 mL, 0.96 mmol) were added. After stirring for 1 h, the ice bath was removed and reaction mixture was stirred at room temperature for another 8 h. The mixture was diluted with EtOAc (10.0 mL) and washed successively with 5% HCl and saturated solutions of CuSO4 and NaHCO3. The organic layer was dried over anhydrous MgSO4 and the solvent removed in a rotary evaporator. the residue was then dissolved in anhydrous benzene (11.0 mL) and DBU (0.16 mL, 1.1 mmol) was added. After refluxing for 48 h, the reaction mixture was cooled to room temperature and the solvent was evaporated to the dryness. The crude product was dissolved in EtOAc (20.0 mL) and was washed with water. The organic layer was dried over anhydrous MgSO4 and the solvent was removed in a rotary evaporator. The residue was chromatographed on silica gel (n-hexane-EtOAc, 85:15) to furnish 15 and 16 (85.3 mg, 54%) in a ratio of 88:12, respectively.
2nd method. To a solution of 14 (73.0 mg, 0.19 mmol) in anhydrous petroleum ether:pyridine (1:1, 2.0 mL) under nitrogen atmosphere, a solution of POCl3 (38 ml, 0.40 mmol) in anhydrous petroleum ether (1.0 mL) was slowly added. After refluxing for 5 h, the mixture was cooled to room temperature; water (10.0 mL) was added and the solution was extracted with EtOAc (2 x 10.0 mL). The crude product was washed with water, dried over MgSO4 and the solvent was removed in a rotary evaporator. The residue was chromatographed on silica gel (n-hexane-EtOAc, 85:15) to furnish a mixture of 15 and 16 (23.0 mg, 33%) as colorless oil in a ratio of 88:12, respectively. IR (film) nmax/cm-1: 2952, 1728, 1436, 1389, 1247, 737; For compound 15: 1H NMR (500 MHz) d: 0.92 (s, 3H, H-18), 0.95 (m, 1H, H-1), 1.22 (s, 3H, H-19), 1.42-1,60 (m, 6H), 1.60 (s, 3H, H-16), 1.63-1.90 (m, 6H), 1.69 (s, 3H, H-17), 1.93 (m, 1H, H-11), 3.18 (m, 1H, H-7), 3,66 (s, 3H, H-21), 3.73 (s, 3H, H-22), 5.04 (bt, J 7.2; 1.0 Hz, 1H, H-13); 13C NMR (125 MHz) d: 14.8 (C-20), 17.0 (C-19), 17.7 (C-2), 18.1 (C-16), 23.8 (C-6), 24.2 (C-17), 25.7 (C-11), 27.0 (C-12), 34.6 (C-10), 36.7 (C-3), 37.6 (C-1), 41.2 (C-5), 46.4 (C-4), 52.0 (C-21), 52.1 (C-22), 53.5 (C-9), 57.2 (C-7), 58.8 (C-8), 125.2 (C-13), 132.2 (C-15), 170.6 (C-14), 176.2 (C-18); For compound 16: 1H NMR (500 MHz) d: 0.93 (s, 3H, H-20), 0.94 (d, J 6.0 Hz, 6H, H-16, H-17); 0.95 (m, 1H, H1), 1.22 (s, 3H, H-19), 1.421.60 (m, 4H), 1.63-1.90 (m, 5H), 2.22 (sept. J 6.5 Hz, 1H, H-15), 2.37 (dd, J 12.0, 7.2 Hz, 2H, H-11), 3.16 (m, 1H, H-7), 3.65 (s, 3H, H-21), 3.66 (s, 3H, H-22), 5.25 (ddd, J 15.8, 7.2, 4.0 Hz, 1H, H-12), 5.41(dd. J 15.8, 6.0 Hz, 1H, H-13); 13C NMR (125 MHz) d: 14.8 (C-20), 17.0 (C-19), 17.7 (C-2), 22.3 (C-16), 22.4 (C-17), 23.7 (C-6), 23.8 (C-11), 31.2 (C-15), 34.6 (C-10), 36.7 (C-3), 37.6 (C-1), 41.2 (C-5), 46.4 (C-4), 52.0 (C-21), 52.1 (C-22), 53.5 (C-9), 57.2 (C-7), 58.9 (C-8), 123.7 (C-12), 139.8 (C-13), 170.6 (C-14), 178.2 (C-18); HRMS: Calcd. for C22H34O5: 378.24062; Found: 378.24157 (M+).
Synthesis of 19 from 17
To a solution of 17 (48.7 mg, 0.13 mmol) dissolved in anhydrous CH2Cl2 (2.5 mL), was added PCC (57.7 mg, 0.26 mmol) at room temperature. After stirring for 8 h, the reaction mixture was filtered through a small pad of silica gel and the solvent was removed in a rotary evaporator. The residue was chromatographed on silica gel (CH2Cl2-MeOH, 95:5) to give 19 (16.0 mg, 30%) as colorless crystals: mp 103-105 °C; [a]D20 -85.0 (c 1.8, CHCl3); IR (film) nmax/cm-1: 546, 3515, 3459, 2932, 1716, 1249, 736; 1H NMR (300 MHz) d: 0.73 (s, 3H, H-20), 0.92 (m, 1H, H-1), 1.10 (d, J 6.8Hz, 3H, H-16), 1.12 (d, J 6.8 Hz, 3H, H-17), 1.26 (s, 3H, H-19), 1.50 (m, 2H, H-2), 1.54 (m, 1H, H-6), 1.60 (m, 1H, H-1'), 1.62 (m, 2H, H-3), 1.70 (m, 1H, H-11), 1.88 (m, 1H, H-6'), 1.90 (m, 1H, H-5), 1.93 (sl, 1H, H-9), 2.35 (dt, J 3.5, 9.4 Hz, 1H, H-11), 2.65 (d, J 2.6 Hz, OH at C-7) 2.87 (sept, J 6.8 Hz, 1H, H-15), 3.15 (ddd, J 13.5, 7.5, 5.2 Hz, 1H, H-12), 3.69 (s, 3H, H-21), 3.79 (s, OH at C-8), 4.35 (dd, J 12.0, 6.0 Hz, 1H, H-7), 4.66 (dd, J 8.2, 2.7 Hz, 1H, H-14), 5.1 (m, OH at C-14); 13C NMR (75 MHz) d: 15.8 (C-20), 16.8 (C-19), 17.2 (C-2), 18.5 (C-16), 18.6 (C-17), 23.5 (C-11), 33.4(C-6), 36.5 (C-10), 36.6 (C-3), 39.0 (C-1), 39.1 (C-15), 46.7 (C-4), 47.1 (C-5), 52.2 (C-21), 53.9 (C-12), 60.0 (C-9), 70.0 (C-7), 75.5 (C-14), 81.0 (C-8), 178.4 (C-18), 214.0 (C-13); HRMS: Calcd for C21H34O6: 382.23554; Found: 382.23553 (M+).
Synthesis of 20 from 17
To a solution of 17 (200.0 mg, 0.56 mmol) in anhydrous CH2Cl2 (15.0 mL) was added 99% mCPBA (192.0 mg, 1.12 mmol) and NaHCO3 (48.0 mg, 0.56 mmol), and mixture was refluxed for 3 days. An additional portion of 99% mCPBA (96.0 mg, 0.56 mmol) and NaHCO3 (24.0 mg, 0.28 mmol) were added, and the mixture was refluxed for another 3 days. The reaction mixture was filtered and the organic phase washed with a saturated NaHCO3 solution. The organic layer was dried over anhydrous MgSO4 and the solvent was removed in a rotary evaporator. The residue was chromatographed on silica gel (CH2Cl2-MeOH, 99:1) to give 20 (167.5 mg, 80%) as colorless crystals: mp 60 -62 °C; [a]D20 -9.3 (c 1.1, CHCl3); IR (film) nmax/cm-1: 3483, 2985, 1725, 1459, 1252, 1106, 736; 1H NMR (300 MHz) d: 0.86 (s, 3H, H-20), 1.19 (m, 1H, H-1), 1.25 (s, 3H, H-19), 1.26 (d, J 6.2 Hz, 3H, H-16), 1.28 (d, J 6.2 Hz, 3H, H-17), 1.48 (m, 1H, H-11), 1.52 (m, 2H, H-2), 1.60 (m, 1H, H-1'), 1.64 (m, 2H, H-3), 1.73 (dd, J 4.5, 1.8 Hz, 2H, H-6), 1.85 (m, 1H, H-9), 1.88 (m, 1H, H-5), 2.00 (ddd, J 12.7, 8.2, 6.2 Hz, 1H, H-11'), 2.30 (d, J 7.7 Hz, OH), 2.60 (dq, J 9.6, 6.2 Hz, 1H, H-12), 3.28 (s, 1H, H-7), 3.67 (s, 3H, H-21), 4.10 (dd, J 10.0; 7.7 Hz, 1H, H-14), 5.08 (sept, J 6.8, 1H, H-15); 13C NMR (75 MHz) d: 15.6 (C-20), 17.4 (C-19), 17.6 (C-2), 21.8 (C-16), 21.9 (C-17), 24.3 (C-11), 24.4 (C-6), 33.5 (C-10), 37.0 (C-3), 38.9 (C-1), 40.6 (C-5), 45.9 (C-4), 51.0 (C-9), 52.2 (C-21), 52.9 (C-12), 56.1 (C-7), 64.2 (C-8), 68.2 (C-15), 73.1 (C-14), 173.4 (C-13), 178.3 (C-18). HRMS: Calcd for C21H32O6: 380.21989; Found: 380.21962 (M+).
This work was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP). C.S. gratefully acknowledges CNPq and FAEP/UNICAMP for fellowships. We also thank Prof. R. Custódio and Mr. A. de L. Machado for assistance with the computational calculations, Prof. L.H.B. Baptistella for helpful discussions and Dr. C.H. Collins for reviewing this article.
1. Ikan, R.; Natural Products: A Laboratory Guide; Israel Universities: Jerusalem, 1969, p.169. [ Links ]
2. Dewick, P. M.; Nat. Prod. Rep. 1997, 14, 111. [ Links ]
3. Haslinger, E.; Hüfner, A.; Monatsh. Chem. 1995, 126, 1109; [ Links ]Kuzakov, E. V.; Shmidt, E. N.; Bagryanskaya, Y.; Gatilov, Y. V.; Chem. Nat. Comp. 1994, 30, 339; [ Links ]Abad, A.; Agullo, M.; Arno, M.; Cuñat, A. C.; Zaragoza, R. J.; J. Org. Chem. 1989, 54, 5123; [ Links ]Abad, A; Arno, M.; Domingo, L.R.; Zaragoza, R.J.; Tetrahedron 1985, 41, 4937; [ Links ]Suryawanshi, S. N.; Rani, A.; Dhami, T. S.; Bhakuni, D. S.; Synth. Commun. 1989, 19, 2927; [ Links ]Steindl, H.; Haslinger, E.; J. Org. Chem. 1985, 50, 3749; [ Links ]Koyama, H.; Okawara, H.; Kobayashi, S.; Ohno, M.; Tetrahedron Lett. 1985, 26, 2685. [ Links ]
5. Akita, H.; Oishi, T.; Chem. Pharm. Bull. 1981, 29, 1580. [ Links ]
6. Santos, C.; Rosso, C. R. S.; Imamura, P. M.; Synth. Commun. 1999, 29, 1903. [ Links ]
7. Hess, S. C., Farah, M. I. S.; Eguchi, S. V.; Imamura, P. M.; J. Braz. Chem. Soc. 2000, 11, 59. [ Links ]
8. Santos, C., PhD Thesis, Universidade Estadual de Campinas, Brazil, 2002. [ Links ]
9. M. John, K. krohu, U. Flörke, H-.J. aust, S. Draeger, B. Schulz, J. Nat. Prod. 1999, 62, 1218. [ Links ]
10. SPARTANâ, version 1.5; Wavefunction Inc., 18041 Von Karman Ave. Suite 370, Irvine CA 92612 USA, 2000. [ Links ]
11. Hyperchem 6.03 professionalâ. Hypercube, Inc., Gainesville, USA, http://www.hyper.com/products/Registration/hyper6.03, accessed in May 2001. [ Links ]
12. Valverde, S.; Lopez, J. C.; Rabanal, R. M.; Escudero, J.; Tetrahedron 1986, 42, 573; [ Links ]
13. Presser, A.; Haslinger, E. Wels, R.; Hüfner, A.; Monatsh. Chem. 1998, 129, 921. [ Links ]
15. Scheidegger, U; Schaffner, K.E.; Jeger, O.; Helv. Chim. Acta 1962, 45, 400. [ Links ]
16. Gesson, J.P.; Jacquesy, J. C.; Renoux, B.; Tetrahedron 1989, 45, 5853. [ Links ]
17. Shimoma, F.; Kondo, H.; Yuuya, S.; Suzuki, T.; Hagiwara, H.; Ando, M.; J. Nat. Prod. 1998, 61, 22. [ Links ]
18. Santos, R. B.; Brocksom, T. J.; Brocksom, U.; Tetrahedron Lett. 1997, 38, 745. [ Links ]
19. Garlaschelli, L.; Vidari, G.; Gazz. Chim. Ital. 1987, 117, 251. [ Links ]
20. Berti, G.; Macchia, B.; Macchia, F.; Tetrahedron Lett. 1965, 6, 3421. [ Links ]
21. Henbest, H. B.; Smith, M.; Thomas, A.; J. Am. Chem. Soc. 1958, 3292. [ Links ]
22. Nwaukwa, S. O.; Keehn, P. M.; Tetrahedron Lett. 1982, 23, 35. [ Links ]
23. Albright, J. D.; Goldman, L.; J. Am. Chem. Soc. 1967, 89, 2416. [ Links ]
24. Cheng, Y. S.; Liu, W. L.; Chen, S.; Synthesis 1980, 223. [ Links ]
25. Leung, W. H.; Wong, T. K.T.; Tran, J. C. H.; Yeung, L. L.; synlett 2000, 677. [ Links ]
Received: February 12, 2003
Published on the web: December 9, 2003
FAPESP helped in meeting the publication costs of this article
This paper is dedicated to Prof. Albert J. Kascheres on occasion of his 60th birthday