Development of a flow through photo-reactor to study degradation of organic compounds by Sequential Injection Analysis (SIA)

Abstracts

This work describes a photo-reactor to perform in line degradation of organic compounds by photo-Fenton reaction using Sequential Injection Analysis (SIA) system. A copper phthalocyanine-3,4',4<FONT FACE=Symbol><FONT FACE=Symbol>²</FONT></FONT>,4<FONT FACE=Symbol><FONT FACE=Symbol>²¢</FONT></FONT>-tetrasulfonic acid tetrasodium salt dye solution was used as a model compound for the phthalocyanine family, whose pigments have a large use in automotive coatings industry. Based on preliminary tests, 97% of color removal was obtained from a solution containing 20 µmol L-1 of this dye.

photo-Fenton reactor; sequential injection analysis; wastewater; copper phthalocyanine dye; automotive coatings industry

Este trabalho descreve um foto-reator para a execução "in line" da degradação de compostos orgânicos através da reação de foto-Fenton, utilizando-se de um sistema de Análise por Injeção Sequencial (SIA). Uma solução do corante do sal tetrassódico de ácido 3,4',4<FONT FACE=Symbol><FONT FACE=Symbol>²</FONT></FONT>,4<FONT FACE=Symbol><FONT FACE=Symbol>²¢</FONT></FONT>-tetrassulfônico de ftalocianina de cobre foi empregada como modelo da família das ftalocianinas, cujos pigmentos apresentam grande utilização na indústria de tintas automotivas. Baseando-se em testes preliminares, uma remoção de cor de 97% foi obtida em uma solução contendo 20 µmol L-1 deste corante.

ARTICLE

Development of a flow through photo-reactor to study degradation of organic compounds by Sequential Injection Analysis (SIA)

Allan C. V. dos Santos; Jorge C. Masini^*

Instituto de Química, Universidade de São Paulo, CP 26077, 05513-970 São Paulo-SP, Brazil

ABSTRACT

This work describes a photo-reactor to perform in line degradation of organic compounds by photo-Fenton reaction using Sequential Injection Analysis (SIA) system. A copper phthalocyanine-3,4',4²,4²¢-tetrasulfonic acid tetrasodium salt dye solution was used as a model compound for the phthalocyanine family, whose pigments have a large use in automotive coatings industry. Based on preliminary tests, 97% of color removal was obtained from a solution containing 20 µmol L^-1 of this dye.

Keywords: photo-Fenton reactor, sequential injection analysis, wastewater, copper phthalocyanine dye, automotive coatings industry.

RESUMO

Este trabalho descreve um foto-reator para a execução "in line" da degradação de compostos orgânicos através da reação de foto-Fenton, utilizando-se de um sistema de Análise por Injeção Sequencial (SIA). Uma solução do corante do sal tetrassódico de ácido 3,4',4²,4²¢-tetrassulfônico de ftalocianina de cobre foi empregada como modelo da família das ftalocianinas, cujos pigmentos apresentam grande utilização na indústria de tintas automotivas. Baseando-se em testes preliminares, uma remoção de cor de 97% foi obtida em uma solução contendo 20 µmol L^-1 deste corante.

Introduction

Oxidative destruction of chemicals provides ultimate solutions for the treatment of hazardous wastes¹. Advanced Oxidative Processes (AOP's) are based on the generation of the hydroxyl radical, ^•OH, a species that acts as a strong oxidant to a wide variety of organic and inorganic compounds². A special kind of AOP is the Fenton reaction,³ in which the hydroxyl radical is formed from the reaction between H₂O₂ and Fe(II) ions. This reaction can also be processed under influence of ultraviolet radiation, leading to the so-called photo-Fenton reaction. The Fenton and related reactions are viewed as potentially convenient and economical ways to generate oxidizing species for treating chemical wastes; compared to other bulk oxidants, hydrogen peroxide and iron are inexpensive, safe, and environmentally friendly⁴. AOP's are particularly interesting for non-biodegradable compounds that can be recalcitrant and/or toxic to microorganisms⁵.

Flow techniques are being consolidated as powerful tools for the routine control of parameters in various samples of environmental origin⁶. Sequential Injection Analysis (SIA), a flow technique proposed by Ruzicka and Marshall, ⁷ can be used to perform experiments necessary for the optimization of industrial processes⁸. The purpose of this paper is to describe an UV reactor specifically designed to study photo-Fenton reactions with a SIA system. A solution of copper phthalocyanine dye was used as a model compound for the phthalocyanine family, whose pigments may be present in effluents of automotive coatings industry.

Experimental

Reagents

All reagents were of analytical grade and the working solutions were prepared in deionized water obtained from a Simplicity 185 system from Millipore coupled to an UV lamp (Millipore, São Paulo, SP, Brazil).

Copper phthalocyanine dye, specifically copper phthalocyanine-3,4',4²,4²¢-tetrasulfonic acid tetrasodium salt, molecular formula C₃₂H₁₂CuN₈O₁₂ S₄.4Na (CAS 123439-80-5) and molar mass of 984.25 g mol^-1 was acquired from Sigma (Sigma-Aldrich Brazil, São Paulo, SP). A 40 µmol L^-1 solution of the dye was prepared only by dilution of the salt with deionized water, adjusting the pH to 2.5 by adding HNO₃.

Iron sulfate solution was prepared by dissolving FeSO₄.7H₂O salt with deionized water and HNO₃ to obtain a final concentration of 80 µmol L^-1 (pH 2.5) and then purged with N₂ and protected from atmospheric O₂, thus avoiding rapid oxidation of Fe(II).

Hydrogen peroxide solution at concentration of 0.5 mmol L^-1 was prepared by direct dilution of commercial stock solution with deionized water and HNO₃ to a final pH of 2.5.

Apparatus and procedure

Reactor design

The photo-reactor used in the experiments reported herewith was designed in the author's laboratory and the steel structure was manufactured under request by SCA Serviços e Caldeiraria (Ribeirão Pires, SP, Brazil). Figure 1A presents the cover (lid) portion of the reactor and Figure 1B describes the body of the reactor, both made of steel. Basically, a mercury lamp is introduced in the center of the lid hole; more precisely, a 9 cm long quartz bulb of a high pressure mercury vapour source obtained from a common street lightning fluorescent lamp (Osram HQL E40, 400 W) by cutting and removing the larger external fluorescent bulb. Similar use of street lamps for a FIA reactor was already reported by Cavicchioli and Gutz⁹. An "U" shaped quartz tube (0.4 cm o.d., 0.3 cm i.d., 35 cm height) was placed inside both cylindrical paths of the cover. Placement of 5 cm of the tube over the lid base allows connection with SIA/detector tubes; the remaining of the tube (30 cm) is positioned inside the illuminated area of the reactor. Two Tygon tubes of distinct internal diameters allowed the connection of the 0.8 mm i.d. Teflon (PolyTetraFluoroEthylene, PTFE) tube from the SIA system to the reactor and from the reactor to the flow cell of the spectrophotometer. The first Tygon tube (0.3 cm o.d., 0.1 cm i.d., 1.3 cm length) is partially inserted (0.3 cm) on the second Tygon Tube (0.4 cm o.d., 0.3 cm i.d., 2.3 cm length) and acts as an intermediate connection. This is necessary because it was empirically observed that a direct connection of a low i.d. PTFE tube to a large i.d. quartz tube leads to air bubbles disruption. Changing the internal diameter (with Tygon connections) and after changing the character of the walls (hydrophobic/hydrophilic), however, leads to stable air bubbles for a wide range of air plugs and flow rates.

An electric fan (Roxline cooler 065.0004.90006) was fitted to the base of the reactor to force air circulation from its bottom, exiting through the curved slit at the cover, and keeping a stable temperature near the quartz tube region.

Externally, the reactor was coated with a commercial low gloss black primer. Because the internal reactor walls are very reflective, the final internal portion of reactor body (from the fan area to the initial curvature of the reactor base) was also coated with the primer to reduce UV scattering to the environment. Figure 2 shows images of the cover apparatus (Fig. 2A) and the reactor final assembly (Fig. 2B).

SIA manifold

A FIAlab 3500 system (FIAlab Instruments, Bellevue, WA, USA) was used in the sequential injection mode according to Figure 3. Solutions were driven by a 5.00 mL syringe pump, SP, and an eight port rotary valve, RV (Valco Instrument Co., Houston). The holding coil, HC, was made of 3 m of 0.8 mm i.d. Teflon (PolyTetraFluoroEthylene, PTFE) tubing. Connection from the reactor to the flow detection cell was 53.5 cm long; connection from port 1 to the reactor was 42 cm long; both were made of 0.8 mm i.d. PTFE tubing. All other tubing connections were made of 0.8 mm i.d. PTFE tubing and PTFE nuts and ferrules (Upchurch, Oak Harbor, WA, USA). A Micronal B382 spectrophotometer (Micronal, São Paulo, SP, Brazil) was used as detector fitted to a 10 mm light path length flow cell with 80 µL of internal volume from Hellma (Hellma GmbH& Co. Mülheimheim, Baden, Germany), performing absorbance measurements at 632 nm. Spectral curve was previously obtained in a Hitachi spectrophotometer model U-3000 using a quartz batch cell of 10 mm optical path length.

The SIA system starts the on line sampling of solutions using the monosegmented flow analysis approach¹⁰. Monosegments aspirated to the holding coil are directed to a mixing chamber for homogenization. Sample, reagents and diluted HNO₃ are mixed to give a final solution containing 20 µmol L^-1 of phthalocyanine at pH 2.5, 140 µmol L^-1 of H₂O₂ and 14 µmol L^-1 of FeSO₄. Another 1.0 mL monosegment containing the reaction mixture of the mixing chamber is aspirated by the SIA system and then pumped toward the photo-reactor and stopped for degradation. After the irradiation time, the monosegment is driven to the spectrophotometer for absorbance reading under stopped flow conditions on the 'plateau' between the two sharp peaks generated by the air bubbles passing through the detector. Cleaning of the manifold is performed automatically by the SIA equipment by pumping the carrier (deionized water) through the system.

Results and Discussion

General performance of photo-reactor

The temperature profile and time for temperature stabilization of the photo-reactor were studied before performing the blue dye degradation. Using an I.R. thermometer and measuring the reactor wall temperature after 20 min it was possible to define the temperature profile (Figure 4); by measuring the temperature time dependence of the hottest region (12 cm below the cover), it was observed that the equipment can reach a stable temperature profile after 15 min (stabilization time) as shown in Figure 5.

Measurement with an ordinary mercury thermometer of water temperature exiting the reactor showed that the system can reach temperature equilibrium between carrier and equipment in less than 15 s. This fast equilibration time is possible due to the high area / volume ratio of the quartz tube. Water temperature was found to be around 60 °C, corresponding to approximately the mean temperature in the reactor path (65 °C).

Copper phthalocyanine dye degradation

Until entrance to the photo-reactor, the solution kept its original blue color, that is, the exposure time of the sample to the ^•OH radical generated in the Fenton reaction was not enough to start a significant color reduction of the dye. Four experiments were performed using a 20 µmol L^-1 dye solution under distinct experimental conditions as detailed in Table 1. Results are graphically summarized in Figure 6. Phthalocyanine dye has a linear absorbance range from 1.0 to 10 µmol L^-1; thus the original solution signal height (experiments #1 and #2) should be about 1.2 times higher than the observed height due to linearity loss. The purpose of the first two experiments is to show that UV irradiation is the main factor involved in color removal of the solution (experiments #3 and #4). Fenton reaction alone (experiment #2) caused a slight reduction in the color of the original solution, but it was not efficient under analysis time. A very strong decrease of the analytical signal is observed when the photo-reactor is operating; if a low concentration of the dye solution (below 20 µmol L^-1) was used, the detector could not be able to register any signal from the remaining dye under operational conditions. Considering the calibration curve, the color removal percentage reached 97%.

Conclusions

Preliminary tests have shown that the proposed photo-reactor could be used to study the degradation of matrices by the photo-Fenton reaction. It also suggests an industrially feasible pre-treatment process because fast color removal was achieved in a very short period of time, a feature that would not be possible by using only direct biological treatments¹¹. Future works will show detailed study for phthalocyanine blue dye and how to get fast and reliable optimization of the degradation conditions with SIA manifolds.¹²

Acknowledgements

The authors are grateful to FAPESP and CNPQ for the financial support. We are also grateful to Carlos Eduardo Rodrigues da Silva and Juilson Nonato Machado da Silva for kindly helping to coat the photo-reactor.

Received: December 8, 2008

Web Release Date: October 9, 2009

FAPESP helped in meeting the publication costs of this article.

• 1. Huang, C. P.; Dong, C.; Tang, Z.; Waste Manage. 1993, 13, 361.
• 2. Nogueira, R. F. P.; Trovó, A. G.; da Silva, M. R. A; Vila, R. D.; Quim. Nova 2007, 30, 400.
• 3. Fenton, H. J. H.; J. Chem. Soc. 1894, 65, 899.
• 4. Pignatello, J. J.; Oliveros, E.; MacKay, A.; Crit. Rev. Environ. Sci. Technol. 2006, 36, 1.
• 5. Raj, C. B. C.; Quen, H. L.; Chem. Eng. Sci. 2005, 60, 5305.
• 6. Miró, M.; Hansen, E. H.; Trends Anal. Chem. 2006, 25, 267.
• 7. Ruzicka, J.; Marshall, G. D.; Anal. Chim. Acta 1990, 237, 329.
• 8. dos Santos, A. C. V.; Masini, J. C.; Talanta 2009, 77, 1081.
• 9. Cavicchioli, A.; Gutz, I. G. R.; Anal. Chim. Acta 2001, 445, 127.
• 10. Pasquini, C.; Oliveira, W. A.; Anal. Chem. 1985, 57, 2575.
• 11. Martínez, N. S. S.; Fernández, J. F.; Segura, X. F.; Ferrer, A. S.; J. Hazard. Mater. 2003, B101, 315.
• 12. dos Santos, A. C. V.; Masini, J. C.; Microchem. J. 2009, 93, 110.

Publication Dates

• Publication in this collection
  14 Oct 2011
• Date of issue
  2009

History

• Accepted
  09 Oct 2009
• Received
  08 Dec 2008