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Brazoides A-D, New Alkaloids from Justicia gendarussa Burm. F. Species

Abstract

Four new alkaloids, Brazoides A-D, together with three known compounds squalene, β-sitosterol and lupeol, were isolated from leaves of Justicia gendarussa. These structures were established by spectrometric techniques, mainly high-resolution electrospray ionization mass spectrometry (HRESIMS) and 1D and 2D nuclear magnetic resonance (NMR), including comparative analysis with literature values. Structural determination of the compounds, Brazoides A-D, was strengthened by molecular modeling and density functional theory (DFT) calculations to predict the NMR data and compare with the experimental NMR values of these natural products. The new compounds were tested against three human cancer cell lines (glioblastoma, prostate and colon), but none exhibited activity.

Keywords:
Acanthaceae; Justicia gendarussa; alkaloids; Brazoides


Introduction

Justicia is the largest genus of Acanthaceae family, with approximately 600 species, distributed throughout the tropics and subtropics of both hemispheres, extending into the temperate regions of North America.11 Corrêa, G. M.; Alcântara, A. F. C.; Braz. J. Pharmacog. 2012, 22, 220.

2 Wasshausen, D. C.; Brittonia 2002, 54, 286.
-33 Kottaimuthu, R.; Phytotaxa 2015, 213, 70. In Brazil, this genus is represented by approximately 128 species.44 Braz, D. M.; Phytotaxa 2015, 236, 184. The species Justicia gendarussa Burm. F. is found in tropical and subtropical areas of Asia and India, and also in northeastern Brazil. It exhibits several biological properties, such as antioxidant, anti-arthritic, anti-inflammatory, analgesic, anti-cancer, hepatoprotective and larvicide. J. gendarussa has been used in folk medicine to treat fever, cough, aphthae, arthritis, headaches, facial paralysis, earache, bronchitis, liver diseases, chronic rheumatism, inflammations, vaginal discharges, dyspepsia and ocular diseases.55 Oliveira, A. F. M.; Andrade, L. H. C.; Acta Amaz. 2000, 30, 569.

6 Sonal, P.; Maitreyi, Z.; Asian J. Tradit. Med. 2011, 6, 61.

7 Senthilkumar, N.; Varma, P.; Gurusubramanian, G.; Parasitol. Res. 2009, 104, 237.

8 Bhagya, N.; Chandrashekar, K. R.; Asian J. Pharm. Clin. Res. 2013, 6, 100.
-99 Uddin, S.; Sinha, S.; Hossain, A.; Kaisar, A.; Hossain, K.; Rashid, M. A.; Dhaka Univ. J. Pharm. Sci. 2011, 10, 53.

The species of the genus Justicia have many morphological similarities among themselves, so that share some common names and medicinal indications. In Brazil J. gendarussa is known as Anador or Anador grande.55 Oliveira, A. F. M.; Andrade, L. H. C.; Acta Amaz. 2000, 30, 569. Earlier phytochemical studies reported the presence of triterpenoids,99 Uddin, S.; Sinha, S.; Hossain, A.; Kaisar, A.; Hossain, K.; Rashid, M. A.; Dhaka Univ. J. Pharm. Sci. 2011, 10, 53. steroids,99 Uddin, S.; Sinha, S.; Hossain, A.; Kaisar, A.; Hossain, K.; Rashid, M. A.; Dhaka Univ. J. Pharm. Sci. 2011, 10, 53.

10 Bhagya, N.; Chandrashekar, K. R.; Kalluraya, B.; Chem. Nat. Compd. 2013, 49, 972.
-1111 Bachheti, R. K.; Pandey, D. P.; Joshi, A.; Rana, V.; Int. J. ChemTech Res. 2011, 3, 244. flavonoides,1111 Bachheti, R. K.; Pandey, D. P.; Joshi, A.; Rana, V.; Int. J. ChemTech Res. 2011, 3, 244.

12 Prajogo, E. W. B.; Guliet, D.; Queiroz, E. F.; Wolfender, J. L.; Cholies, Z. N.; Aucky, H.; Hostettmann, K.; Folia Med. Indonesiana 2009, 45, 28.
-1313 Wahi, S. P.; Wahi, A. K.; Kapoor, R.; JRIM 1974, 9, 65. and nitrogen compounds in its leaves.1414 Kiren, Y.; Deguchi, J.; Hirasawa, Y.; Morita, H.; Prajogo, B.; J. Nat. Med. 2014, 68, 754.,1515 Chakravarty, A. K.; Dastida, R. P. P. G.; Pakrash, S. C.; Tetrahedron 1982, 38, 1797.

In this paper, we are describing the isolation and the structure elucidation of four new alkaloids, named Brazoides A-D (1-4), of Justicia gendarussa (Figure 1). The structures were established by spectrometric techniques, mainly high-resolution electrospray ionization mass spectrometry (HRESIMS) and 1D and 2D nuclear magnetic resonance (NMR), including comparative analysis with literature values. Structural determination of the compounds was strengthened by molecular modeling and density functional theory (DFT) calculations to predict the NMR data and compare with the experimental NMR values of these natural products. Cytotoxic activities in vitro of compounds were evaluated using three human cancer cell lines, SF-295 (glioblastoma), PC-3 (prostate) and HCT-116 (colon).

Figure 1
Alkaloids isolated from J. gendarussa.

Experimental

General experimental procedures

Optical rotations were obtained on a JASCO P-200 polarimeter. Infrared spectra (IR) were recorded on a PerkinElmer Spectrum 1000 FT-IR spectrometer using KBr pellets. The NMR spectra were accomplished on Bruker Avance DRX-500 and Agilent VNMR-600 spectrometers. The 1H and 13C chemical shifts are expressed in the d scale and were referenced to TMS through the residual solvent. HRESIMS were obtained on a Shimadzu spectrometer LCMS-IT-TOF type equipped with electrospray ionization source. Column chromatographies were performed with silica gel 60 (63-200 µM, Vetec), Sephadex LH-20 (Pharmacia) and cartridge SPE C18 (Phenomenex). Thin layer chromatography (TLC) was performed on precoated silica gel aluminum sheets (Macherey-Nagel) with fluorescent indicator 254 nm. The compounds were visualized by UV detection and by spraying with vanillin/perchloric acid/EtOH solution, followed by heating. High performance liquid chromatography (HPLC) separations were carried out on a Shimadzu apparatus equipped with SPD-M20A UV-Vis detector, and a Phenomenex® Luna 5u C18 preparative column (250 × 10 mm), using a H2O/MeOH solvent system as mobile phase.

Plant material

The leaves of J. gendarussa, were collected in the Garden of Medicinal Plants of the Federal University of Ceará (UFC) in March 2012. A voucher specimen was identified by the botanist Edson Nunes deposited in the Herbarium Prisco Bezerra, of UFC with the number 52214.

Extraction and isolation

The dried and crushed leaves of J. gendarussa (295.0 g) were extracted exhaustively with ethanol at room temperature and then evaporated under reduced pressure to give 55.4 g of crude extract. Afterwards, this extract was subjected to removal of chlorophyll. The chlorophyll extraction was performed by dripping of water on the ethanol extract under stirring until obtaining a ethanol-water (7:3) solution. Subsequently this solution was stored at low temperature for 24 hours and then vacuum filtered, yielding a precipitate rich in chlorophyll and a free ethanol extract chlorophyll (46.0 g).1616 Ferri, P. H. In Plantas Medicinais: Arte e Ciência - Um Guia de Estudo Interdisciplinar; Di Stasi, L. C., ed.; Editora UNESP: São Paulo, p. 129. Approximately 20.0 g of chlorophyll-free crude extract was subjected to column chromatography on silica gel eluted with mixtures of solvents: hexane, CH2Cl2, EtOAc and MeOH in increasing polarity, giving seven fractions. After removing the solvent, the major fraction AcOEt/MeOH (50:50, v/v) eluate (12.0 g), was separated by silica gel column eluted with CH2Cl2 containing increasing percentages of MeOH, providing eight fractions (F1-F8). The fraction F1 (85.0 mg) was chromatographed over Sephadex LH 20 eluted with MeOH, affording 95 fractions. Fractions 43-76 were similar in TLC and were combined to give 50.0 mg of a mixture of compounds. Later on, this mixture was chromatographed by semi-preparative RP-18 HPLC, using H2O/MeOH (80:20, v/v) isocratic elution, and a flow of 2.3 mL min-1, to afford 1 (2.5 mg) and 2 (2.0 mg). The fraction F6-7 (4.0 g) was chromatographed on a C18 cartridge eluting H2O/MeOH (50:50, v/v) resulting in 47 fractions. Subsequently, the fraction 1 (615.0 mg) was subjected to a C18 cartridge eluted with H2O/MeOH in gradient of increasing MeOH (0-100%), resulting in 68 fractions. Fractions 4-6 were similar in TLC and were combined to give 80.0 mg of a mixture of compounds. Then, this mixture was separated by semi-preparative RP-18 HPLC using the previous methodology, to afford 3 (23.0 mg) and 4 (13.0 mg).

Calculated NMR

The Spartan'14 software (Wavefunction, Inc.) provides new options for NMR calculations. To know which method would be better for offer values closer to the experimental, the DFT1717 Hohenberg, P.; Kohn, W.; Phys. Rev. B 1964, 136, 864. and the Hartree-Fock (HF)1818 Hartree, D. R.; The Calculation of Atomic Structure; Wiley: New York, USA, 1957. methods were tested with reference compounds from the literature, to define which one would be the most appropriate. After these tests, DFT was the best method, chosen for this work. These ligands structures were constructed and energy-minimized first with the PM6 method.1919 Stewart, J. J. P.; J. Mol. Model. 2007, 13, 1172. Then, DFT method was used for a second minimization, considering the presence of a solvent, preferably the one used in the experimental procedure, and the generation of the NMR spectra.

Cytotoxic activity

The cytotoxic activity was performed using tumor cell lines, SF-295 (glioblastoma), PC-3 (prostate) and HCT-116 (colon) (National Cancer Institute, USA). The cell lines were cultured in RPMI 1640 supplemented with 10% fetal bovine serum and 1% antibiotic, which were incubated at 37 °C and in an atmosphere containing 5% CO2. Samples were diluted in DMSO and tested at a concentration of 5 µg mL-1. Cells were plated at a concentration of 0.1 × 106 cells mL-1 for strains SF-295 and PC-3 and 0.7 × 105 cells mL-1 for the strain HCT-8 and incubated for 72 hours in an oven. Subsequently the samples were centrifuged and the supernatant was removed. Then 150 µL of 3-(4,5-dimethyl-2-thiazol)-2,5-diphenyl-2H-tetrazolium bromide (MTT) solution were added and the plates were incubated for 3 h. The absorbance was measured after dissolution of the precipitate with 150 µL of DMSO in plate spectrophotometer at 595 nm. Cell viability was evaluated by reduction of the yellow dye (MTT) to a blue product as described by Mosmann.2020 Mosmann, T.; J. Immunol. Methods 1983, 65, 55. Doxorubicin was used as a positive control.

Brazoide A (1)

Amorphous white solid; mp 199-203 °C; [α]D2020 Mosmann, T.; J. Immunol. Methods 1983, 65, 55. +16.5° (c 0.1, MeOH); IR (KBr) νmax/ cm-1 3328, 2915, 2851, 1675, 1603, 1453, 1411, 1233, 1083; 1H (600 MHz, MeOH) and 13C (150 MHz, MeOH) NMR data, see Table 1; HRESIMS (positive mode) m/z, calcd. for C17H23NO8 [M + Na]+: 392.1316, found: 392.1315.

Table 1
1H (600 MHz) and 13C (150 MHz) NMR for Brazoide A (1) and Brazoide B (2), including results obtained by heteronuclear 2D shift-correlated HSQC (1JCH) and HMBC (nJCH, n = 2 and 3), in MeOH-d4 as solvent. Chemical shifts (δ, ppm) and coupling constants (J, Hz) in parenthesisa a Number of hydrogens bound to carbon atoms deduced by comparative analysis of {1H}- and APT-13C NMR spectra. Chemical shifts and coupling constants (J) obtained of 1D 1H NMR spectrum. Superimposed 1H signals are described without multiplicity and chemical shifts deduced by HSQC, HMBC and 1H-1H COSY spectra.

Brazoide B (2)

Yellow resin; [α]D2020 Mosmann, T.; J. Immunol. Methods 1983, 65, 55. +14.43° (c 0.1, MeOH); IR (KBr) νmax/ cm-1 3419, 2909, 2847, 1723, 1661, 1453, 1463, 1422, 1245, 1068; 1H (600 MHz, MeOH) and 13C (150 MHz, MeOH) NMR data, see Table 1; HRESIMS (positive mode) m/z, calcd. for C17H23NO7 [M + Na]+: 376.1367, found: 376.1362.

Brazoide C (3)

Yellow resin; [α]D2020 Mosmann, T.; J. Immunol. Methods 1983, 65, 55. -2.2° (c 0.1, MeOH); IR (KBr) νmax/ cm-1 3364, 2982, 2923, 1725, 1646, 1503, 1443, 1298, 1233; 1H (500 MHz, MeOH) and 13C (125 MHz, MeOH) NMR data, see Table 4; HRESIMS (positive mode) m/z, calcd. for C18H27N2O9 [M + H]+: m/z 415.1711, found: 415.1738; HRESIMS (negative mode) m/z, calcd. for C18H25N2O9 [M - H]-: 413.1566, found: 413.1560.

Table 2
Comparison of 13C NMR experimental data for 1 and 2, with calculated DFT-NMR
Table 3
Comparison of 1H NMR experimental data for 1 and 2, with calculated DFT-NMR
Table 4
1H (500 MHz) and 13C (125 MHz) NMR for Brazoide C (3) and Brazoide D (4), including results obtained by heteronuclear 2D shift correlated HSQC (1JCH) and HMBC (nJCH, n = 2 and 3), in MeOH-d4 as solvent. Chemical shifts (δ, ppm) and coupling constants (J, Hz) in parenthesisa a Number of hydrogens bound to carbon atoms deduced by comparative analysis of {1H}- and APT-13C NMR spectra. Chemical shifts and coupling constants (J) obtained of 1D 1H NMR spectrum. Superimposed 1H signals are described without multiplicity and chemical shifts deduced by HSQC, HMBC and 1H-1H-COSY spectra.

Brazoide D (4)

Yellow resin; [α]D2020 Mosmann, T.; J. Immunol. Methods 1983, 65, 55. +14.66° (c 0.1, MeOH); IR (KBr) νmax/ cm-1 3357, 2972, 2822, 1732, 1667, 1624, 1453, 1411, 1297, 1254; 1H (500 MHz, MeOH) and 13C (125 MHz, MeOH) NMR data, see Table 4; HRESIMS (positive mode) m/z, calcd. for C18H27N2O8 [M + H]+: 399.1762, found: 399.1774; HRESIMS (positive mode) m/z, calcd. for C18H25N2O8 [M - H]-: 397.1616, found: 397.1610.

Results and Discussion

Successive chromatographic columns of the EtOH extract of J. gendarussa leaves followed by HPLC led to the isolation of compounds 1-4. The structures of the compounds were determined using IR, NMR (1D and 2D), HRESIMS and molecular modeling.

Compound 1 was isolated as a yellow resin. The molecular formula C17H23NO8 (seven degrees of unsaturation) was deduced from quasi-molecular ion peak at m/z 392.1315 ([M + Na]+, calcd. m/z 392.1316), observed in the HRESIMS, in conjunction with the 1H NMR (1D and 2D 1H-1H correlation spectroscopy (COSY)) spectra and 13C NMR data deduced by experiments ({1H} and heteronuclear single quantum correlation (HSQC)) that revealed the presence of two methyl groups, three methylenes (all sp33 Kottaimuthu, R.; Phytotaxa 2015, 213, 70., including one oxygenated at δC 63.0), six methine carbons [three sp33 Kottaimuthu, R.; Phytotaxa 2015, 213, 70. oxygenated represented by two signals at δC 86.9 (CH-1') and 69.0 (CH-10 and CH-12) and three sp22 Wasshausen, D. C.; Brittonia 2002, 54, 286. aromatic carbons], along with quaternary carbon atoms (five sp22 Wasshausen, D. C.; Brittonia 2002, 54, 286. carbons including two carbonyl groups at δC 173.9 and 176.0, one oxygenated at δC 157.4, one nitrogenated at δC 135.5 and one sp33 Kottaimuthu, R.; Phytotaxa 2015, 213, 70. oxygenated at δC 82.9) (Table 1). The absorption bands in the IR spectrum at νmax 3328, 1675, 1603 cm-1 showed the presence of hydroxyl and carbonyl groups.

The 1H NMR spectrum showed signals attributed to aromatic hydrogens as two doublets at δH 7.05 (meta-coupling, J 2.4 Hz, H-2) and 7.09 (ortho-coupling, J 8.4 Hz, H-5), as well as double doublet at δH 6.82 (meta/ortho-coupling, J 2.4 and 8.4 Hz, H-4), compatible with trisubstituted aromatic ring. It was also observed signals corresponding to oxymethine and methyl hydrogens, among which the signal at δH 4.00 (integration for two hydrogens H-10 and H-11), and δH 1.15 (integration to six hydrogens for two methyl, 3H-11 and 3H-13), was attributed to two equivalents of hydroxy-ethyl groups confirmed by correlations revealed by 1H-1H COSY spectrum, between δH 4.00 (H-10/H-12) and δH 1.15 (H-11/H-13) (Figure 2).

Figure 2
Structure of Brazoide A and key HMBC (H→C), NOESY (H↔H) and COSY (H-H) correlations.

All proton and carbon signals were fully assigned through HSQC and heteronuclear multiple bond correlation (HMBC) spectra analyses (Table 1).

The HMBC cross-peaks revealed the location of the hydroxyl on carbon C-5 and the two carbonyl groups at positions C-8 and C-4', summarized in Table 1 and Figure 2. The relative configuration of 1 was deduced from analysis of nuclear Overhauser effect spectroscopy (NOESY) correlations between the signals at δH 5.11 (H-7) and δH 7.05 (H-6), between H-7 and δH 5.46 (H-1'), and between H-1' and δH 7.09 (H-3) (Figure 1). Chemical calculations based on DFT can be used to provide reliable prediction of NMR parameters (1H and 13C NMR chemical shifts). Initially, by NMR and mass spectroscopy was proposed to compound 1, three possible structures that have the same molecular formula (C17H23NO8) and molecular weight (calcd. m/z 392.1316, [M + Na]+). These structures showed compatibility with all NMR data presented. Thus the prediction of NMR data by DFT was important to define the structure, which was confirmed by comparison of the NMR experimental data with the NMR results of the theoretical model (Tables 2 and 3), and comparison with data reported in the literature.1414 Kiren, Y.; Deguchi, J.; Hirasawa, Y.; Morita, H.; Prajogo, B.; J. Nat. Med. 2014, 68, 754. The structure of 1 was elucidated as the new alkaloid named Brazoide A in honor of the renowned researcher Raimundo Braz-Filho.

Compound 2 (Brazoide B) was obtained as a yellow resin, and was assigned the molecular formula C17H23NO7 corresponding to molecular quasi-molecular ion peak at m/z 376.1362 ([M + Na]+, calcd. m/z 376.1367), observed in the HRESIMS, in conjunction with the 13C NMR data (Table 1). Spectroscopic data of 2 showed to be very similar to 1 (Table 1), but the former one had a different pattern of NMR signals in the aromatic region and the absence of the hydroxylated aromatic carbon signal in the 1H and 13C NMR spectra, respectively. The structure of this compound was confirmed by 2D NMR and the comparison with results of theoretical models (Tables 2 and 3).

Compound 3, a yellow resin, gave the molecular formula C18H26N2O9 from its positive-mode HRESIMS m/z 415.1738 ([M + H]+ calcd. m/z 415.1711), and negative-mode HRESIMS m/z 413.1560 ([M - H]- calcd. m/z 413.1566). The NMR spectra of 3 (Table 4) also showed many similarities with the compound 1, but it was observed an additional signal of a carbonyl carbon at δC 174.0 (C-5'). This signal was not observed in the 13C NMR spectrum, but the HMBC cross-peak between δH 3.67 (H-4') and δC 174.3 (C-5') revealed the presence of the carbonyl group, and has been confirmed by HRESIMS. The analyzed spectroscopic data and comparison with data from the literature1414 Kiren, Y.; Deguchi, J.; Hirasawa, Y.; Morita, H.; Prajogo, B.; J. Nat. Med. 2014, 68, 754. and previously characterized compounds, allowed to identify the compound 3 named Brazoide C.

Compound 4, a yellow resin, gave the molecular formula C18H26N2O8 from its positive-mode HRESIMS m/z 399.1774 ([M + H]+ calcd. m/z 399.1762), and negative-mode HRESIMS m/z 397.1610 ([M - H]- calcd. m/z 397.1616). Compound 4 also showed to be very similar to those compounds described earlier. In comparison to 3 (Table 4), the only difference observed in the NMR spectra was the absence of the hydroxyl signals at C-5. Thus, the compound 4 was assigned as being Brazoide D. Also isolated were the three known compounds, squalene,2121 Cursino, L. M. C.; Mesquita, A. S. S.; Mesquita, D. W. O.; Fernandes, C. C.; Pereira Júnior, O. L.; Amaral, I. L.; Nunez, C. V.; Acta Amaz. 2009, 39, 171. lupeol2222 Silva, F. O.; Oliveira, I. R.; Silva, M. G. V.; Braz-Filho, R.; Quim. Nova 2010, 33, 1874. and the mixture of β-sitosterol and stigmasterol.2323 Souza, A. D. L.; Rocha, A. F. I.; Pinheiro, M. L. A. B.; Andrade, C. H. S.; Galotta, A. L. A. Q.; Santos, M. P. S. S.; Quim. Nova 2001, 24, 439.

Compounds Brazoides A-D were subjected to cytotoxicity tests against three strains, SF-295 (glioblastoma), PC-3 (prostate) and HCT-116 (colon), however none of the compounds showed activity against cell lines tested.

Conclusions

In this work four new alkaloides (1-4) were isolated from the EtOH extract of leaves of J. Gendarussa Burm. F. in addition to a three known compounds. The alkaloids did not show satisfactory results when subjected to cytotoxicity testing.

Acknowledgments

The authors thank CNPq/CAPES/PRONEX/FUNCAP for the fellowships and financial support. We also thank to CENAUREMN, of the Universidade Federal do Ceará for the NMR data, and to Embrapa Agroindustria Tropical-CE for NMR and high-resolution mass spectra.

References

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    Corrêa, G. M.; Alcântara, A. F. C.; Braz. J. Pharmacog. 2012, 22, 220.
  • 2
    Wasshausen, D. C.; Brittonia 2002, 54, 286.
  • 3
    Kottaimuthu, R.; Phytotaxa 2015, 213, 70.
  • 4
    Braz, D. M.; Phytotaxa 2015, 236, 184.
  • 5
    Oliveira, A. F. M.; Andrade, L. H. C.; Acta Amaz. 2000, 30, 569.
  • 6
    Sonal, P.; Maitreyi, Z.; Asian J. Tradit. Med. 2011, 6, 61.
  • 7
    Senthilkumar, N.; Varma, P.; Gurusubramanian, G.; Parasitol. Res. 2009, 104, 237.
  • 8
    Bhagya, N.; Chandrashekar, K. R.; Asian J. Pharm. Clin. Res. 2013, 6, 100.
  • 9
    Uddin, S.; Sinha, S.; Hossain, A.; Kaisar, A.; Hossain, K.; Rashid, M. A.; Dhaka Univ. J. Pharm. Sci. 2011, 10, 53.
  • 10
    Bhagya, N.; Chandrashekar, K. R.; Kalluraya, B.; Chem. Nat. Compd. 2013, 49, 972.
  • 11
    Bachheti, R. K.; Pandey, D. P.; Joshi, A.; Rana, V.; Int. J. ChemTech Res. 2011, 3, 244.
  • 12
    Prajogo, E. W. B.; Guliet, D.; Queiroz, E. F.; Wolfender, J. L.; Cholies, Z. N.; Aucky, H.; Hostettmann, K.; Folia Med. Indonesiana 2009, 45, 28.
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    Wahi, S. P.; Wahi, A. K.; Kapoor, R.; JRIM 1974, 9, 65.
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    Kiren, Y.; Deguchi, J.; Hirasawa, Y.; Morita, H.; Prajogo, B.; J. Nat. Med. 2014, 68, 754.
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    Chakravarty, A. K.; Dastida, R. P. P. G.; Pakrash, S. C.; Tetrahedron 1982, 38, 1797.
  • 16
    Ferri, P. H. In Plantas Medicinais: Arte e Ciência - Um Guia de Estudo Interdisciplinar; Di Stasi, L. C., ed.; Editora UNESP: São Paulo, p. 129.
  • 17
    Hohenberg, P.; Kohn, W.; Phys. Rev. B 1964, 136, 864.
  • 18
    Hartree, D. R.; The Calculation of Atomic Structure; Wiley: New York, USA, 1957.
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    Stewart, J. J. P.; J. Mol. Model. 2007, 13, 1172.
  • 20
    Mosmann, T.; J. Immunol. Methods 1983, 65, 55.
  • 21
    Cursino, L. M. C.; Mesquita, A. S. S.; Mesquita, D. W. O.; Fernandes, C. C.; Pereira Júnior, O. L.; Amaral, I. L.; Nunez, C. V.; Acta Amaz. 2009, 39, 171.
  • 22
    Silva, F. O.; Oliveira, I. R.; Silva, M. G. V.; Braz-Filho, R.; Quim. Nova 2010, 33, 1874.
  • 23
    Souza, A. D. L.; Rocha, A. F. I.; Pinheiro, M. L. A. B.; Andrade, C. H. S.; Galotta, A. L. A. Q.; Santos, M. P. S. S.; Quim. Nova 2001, 24, 439.

Supplementary Information

The supplementary information is available free of charge on the http://jbcs.org.br as a PDF file.

Publication Dates

  • Publication in this collection
    July 2017

History

  • Received
    18 Aug 2016
  • Accepted
    04 Nov 2016
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