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versión impresa ISSN 0103-507X
Rev. bras. ter. intensiva vol.24 no.2 São Paulo abr./jun. 2012
ORIGINAL ARTICLES - CLINICAL RESEARCH
Prevalence and outcomes of infections in Brazilian ICUs: a subanalysis of EPIC II study
Eliézer SilvaI; Luiz Dalfior JuniorI; Haggéas da Silveira FernandesI; Rui MorenoII; Jean-Louis VincentIII
IIntensive Care Unit, Hospital Israelita
Albert Einstein HIAE - São Paulo (SP), Brazil
IIUCIC, Hospital de São José, Centro Hospitalar de Lisboa Central, E.P.E. - Lisboa, Portugal
IIIDepartment of Intensive Care, Erasme Hospital, Université Libre de Bruxelles - Belgium
OBJECTIVE: To determine the
prevalence of infections in Brazilian intensive care units and the associated
mortality by analyzing the data obtained in the Extended Prevalence of
Infection in Intensive Care (EPIC II) study.
METHODS: EPIC II was a multicenter, international, cross-sectional prospective study of infection prevalence. It described the demographic, physiological, bacteriological, and therapeutic characteristics, outcome up to the 60th day, prevalence of infection, and mortality of all the patients admitted to the participating ICUs between zero hour and midnight on May 8, 2007. A total of 14,414 patients were included in the original study. Of these 14,414 patients, 1,235 were Brazilian and were hospitalized in 90 Brazilian ICUs. They represent the focus of this study.
RESULTS: Among these 1,235 Brazilian patients, 61,6% had an infection on the day of the trial, and the lungs were the main site of infection (71.2%). Half of the patients had positive cultures, predominantly gram-negative bacilli (72%). On the day of the study, the median SOFA score was 5 (3-8) and the median SAPS II score was 36 (26-47). The infected patients had SOFA scores significantly higher than those of the non-infected patients 6 (4-9) and 3 (2-6), respectively). The overall ICU mortality rate was 28.4%: 37.6% in the infected patients, and 13.2% in the non-infected patients (p<0.001). Similarly, the in-hospital mortality rate was 34.2%, with a higher rate in the infected than in the non-infected patients (44.2% vs. 17.7%) (p<0.001). In the multivariate analysis, the main factors associated with infection incidence were emergency surgery (OR 2.89, 95%CI [1.72-4.86], p<0.001), mechanical ventilation (OR 2.06, 95% CI [1.5-2.82], p<0.001), and the SAPS II score (OR 1.04, 95% CI [1.03-1.06], p<0.001). The main factors related to mortality were ICC functional class III/ IV (OR 3.0, 95% CI [1.51-5.98], p<0.01), diabetes mellitus (OR 0.48, 95% CI [0.25-0.95], p<0.03), cirrhosis (OR 4.62, 95% CI [1.47-14,5], p<0.01), male gender (OR 0.68, 95% CI [0.46-1.0], p<0.05), mechanical ventilation (OR 1.87, 95% CI [1.19-2.95], p<0.01), hemodialysis (OR 1.98, 95% CI [1.05-3.75], p<0.03), and the SAPS II score (OR 1.08, 95% CI [1.06-1.10], p<0.001).
CONCLUSION: The present study revealed a higher prevalence of infections in Brazilian ICUs than has been previously reported. There was a clear association between infection and mortality.
Keywords: Sepsis; Prevalence; Mortality; Infection; Intensive care units
Infection is associated with high morbidity, mortality, and costs in intensive care units (ICUs). Most infected patients develop a systemic condition characterized as sepsis, which is the leading cause of death in noncardiac ICUs and the 10th leading cause of death in the United States.(1-4) Moreover, some epidemiological studies have found yearly severe sepsis incidences as high as three patients per 1000.(1)
The most important epidemiological studies of infection have been conducted in the United States, Europe, and Australia and have not generally included developing countries.(2) In Brazil, two major studies have evaluated the incidence density of and mortality from severe sepsis in local ICUs. The BASES study was conducted between May 2001 and January 2002. It was a multicenter observational study of 1,383 patients from five Brazilian ICUs (one private and four public hospitals). It reported sepsis, severe sepsis, and septic shock incidence densities of 61.4 (95% CI [55.5 - 67.3]), 35.6 (95% CI [31.1 - 40.1]), and 30 (95% CI [25.9 - 31.4]) per 1,000 patient-days, respectively. The mortality rates for sepsis, severe sepsis, and septic shock were 33.9%, 46.9% and 52.2%, respectively.(5) The second study was also prospective and multicenter. It evaluated a total of 3,128 patients from 75 ICUs in 65 hospitals throughout Brazil and reported sepsis, severe sepsis, and septic shock incidences of 19.6%, 29.6%, and 50.8%, respectively. The overall mortality rate was 46.6% (95% CI [42.5 - 51], with mortalities in the sepsis, severe sepsis, and septic shock subgroups of 16.7%, 34.4%, and 65.3%, respectively.(6)
However, there is little Brazilian data on the prevalence of ICU infections and on the impact they have on outcomes in these patients. In addition, the available data were collected at the beginning of the last decade. Since then, there have been changes in care practices resulting from campaigns to prevent infections in these environments and from improved treatment of septic patients that may have impacted the clinical outcome.
Given these considerations, the present study aimed to determine the infection prevalence and its associated mortality by analyzing data from the Brazilian patients included in the Extended Prevalence of Infection in Intensive Care (EPIC II) study.
This study evaluated Brazilian data from the EPIC II study.(7) EPIC II was a multicenter, international, prospective study of the prevalence of ICU infections. It examined the demographic, physiological, bacteriological, and therapeutic characteristics and the outcomes.(7) A total of 14,414 patients (13,796 of whom were over 18 years of age) were included in the original study, and 1,235 of these patients were Brazilian.(7)
The ICUs were invited to participate through correspondence and advertisements at congresses and international symposiums. Study participation was entirely voluntary, and it was not funded. Approval by the local ethics committee of each participating center was either granted or not necessary due to the purely observational nature of the study.
Demographic, physiological, bacteriological, and therapeutic data were collected from all of the patients admitted to the participating ICUs between zero hour and midnight on May 8, 2007. The Simplified Acute Physiology Score II (SAPS II)(8) and Sequential Organ Failure Assessment (SOFA)(9) scores were obtained on the day of the study. The data recording used specific forms that were returned via website, fax, or mail. Patient follow-up was performed until hospital discharge or until 60 days after admission (July 9, 2007), and the outcomes obtained in the ICUs and during hospitalization were recorded. Any decisions to withdraw or withhold therapy during the ICU stay were recorded. An exclusive phone line was used for questions during the follow-up period. The centers with incomplete data were contacted in an attempt to recover the information, and missing data were not included in the analysis.
Infection was defined according to the classification system of the International Sepsis Forum(10) and validated by the attending physician. The patients who were admitted to the ICU in the immediate postoperative period and who had been there for four weeks or less on the day of the study were considered to be surgical admissions. A surgery scheduled more than 24 hours before its completion was considered an elective surgery, whereas an emergency surgery was performed less than 24 hours after it was indicated. Trauma admissions were those directly associated with a traumatic event, the complications of trauma, and traumas occurring within 30 days of the event. All other admissions were considered to be medical.
The presence of the following comorbidities were recorded: chronic obstructive pulmonary disease, metastatic cancer (metastasis confirmed by surgery or imaging), cirrhosis of the liver, heart failure (New York Heart Association class III and IV), hematologic malignancy (lymphoma, acute leukemia, and multiple myeloma), infection with the human immunodeficiency virus (HIV) (HIV-positive patients with clinical complications, such as Pneumocystis jirovecii, Kaposi's sarcoma, lymphoma, tuberculosis, or toxoplasmosis), chronic renal failure (need for dialysis support or history of chronic renal failure with serum creatinine levels greater than 3.6 g/dL), immunosuppression (a dose of at least 0.3 mg/Kg/day of prednisolone or the equivalent for at least one of the six months prior to the ICU admission), severe malnutrition, congenital humoral or cellular immunodeficiency, radiotherapy or chemotherapy (in the six months prior to ICU admission), and insulin-dependent diabetes mellitus.
All of the data were analyzed at the Department of Intensive Care, University of Brussels, Belgium, in collaboration with the University of Jena, Germany. SSPS version 13.0 (SSPS Inc., Chicago, Illinois) was used for the analysis. Only data from the adult patients (>18 years of age) were used.
The Kolmogorov-Smirnov test, histograms, and normal-quantile plots were used to check for significant deviations from normality in the continuous variables. Nonparametric tests were used to evaluate the variables that did not exhibit a normal distribution. The differences between groups were examined using analysis of variance: the Kruskal-Wallis test, the "t" test, the Mann-Whitney test, the χ2 test, and Fisher's exact test, as appropriate. The Bonferroni correction was used for multiple comparisons.
A multivariable logistic regression analysis was used to determine the risk factors for infection and mortality. The following variables were investigated as independent risk factors for infection: type of admission, place of origin, comorbidities, age, sex, mechanical ventilation, hemodialysis or hemofiltration, and SAPS II score. These variables were also used as risk factors for hospital mortality in the patients who had infections, and this analysis was repeated for the subgroups defined by infection with various microorganisms.
Odds ratios were adjusted for hospital and organization-related factors, including type of ICU (open vs. closed, community vs. university, and medical vs. surgical), number of ICU beds, number of nurses and physiotherapists, continuous presence of an attending physician, consumption of health care supplies (2006 data from the information system of the World Health Organization obtained from their website, http://www.who.int/whosis/whostat/EN_WHS09_Full.pdf, pre-study length of ICU stay, and demographic region. The Cochran-Armitage trend test was used to assess the association of infection rate, the national health-related gross domestic product, SAPS II scores, and SOFA scores with length of ICU stay prior to study initiation. The data were presented as mean (95% confidence interval), median (interquartile range - IQR) or number (%), as appropriate. All of the statistical tests were two-tailed, and p<0.005 was considered statistically significant.
The EPIC II study recruited a total of 14,414 patients, of whom 13,796 were used for the analysis (older than 18 years), from a total of 1,265 ICUs in 75 countries. For the entire population, 51% were considered infected, 71% received antibiotics (even if only for prophylaxis), and 64% had pulmonary infections; the ICU and hospital mortality rates were 18.2% and 24.2%, respectively.(7)
A total of 1,235 patients were treated in 90 Brazilian ICUs and their clinical data are presented in the table 1. Of the 1,235 patients, 761 (61.6%) were considered infected on the day of the study. These patients had higher SAPS II and SOFA scores on ICU admission than did the non-infected patients. Regarding the site of infection, 71.2% of the infected patients had lung infections, 16.6% had urinary tract infections, 13.4% had abdominal infections, and 10.1% had systemic infections. Of those infected, 50.7% had microbiological isolations, of which 33.9% were gram-positive, 72% were gram-negative, and 14.5% were from fungal or other organisms, as shown in table 2. The most common gram-positive cocci were Staphylococcus aureus (16.6%), 7.3% of which were methicillin-resistant Staphylococcus aureus (MRSA), and Staphylococcus epidermidis (11.1%). Among gram-negative bacilli, Pseudomonas sp accounted for 24.6%, Klebsiella sp accounted for 18.7%, and Acinetobacter sp accounted for 15.3%.
As shown in table 3, there was a direct relationship between the severity of patient illness, as assessed by the severity (SAPS II) and organ dysfunction (SOFA) scores and the chance of acquiring an infection.
The infected patients had a higher incidence of organ dysfunction than did the non-infected patients, and the difference was statistically significant. The most prevalent organ dysfunctions were respiratory (62.9%), neurological (43.8%), and renal (31.3%). Other less prevalent disorders were circulatory (27.3%), coagulation (16.9%), and hepatic (12.8%).
The factors associated with higher infection rates in the multivariable logistic regression were emergency surgery (OR=2.89, 95% CI [1.72 - 4.86], p<0.001), mechanical ventilation (OR=2.06, 95% CI [1.5 - 2.82], p<0.001), and the SAPS II score (OR=1.04, 95% CI [1.03 - 1.06], p<0.001) (Table 4).
The overall mortality rate in ICU was 28.4%; this rate was 37.6% for the infected patients, and it was 13.2% for the non-infected patients (p<0.001). The overall hospital mortality rate was 34.2%; this rate was 44.2% for the infected patients, and it was 17.7% for the non-infected patients (p<0.001).
The infected patients had longer ICU stays (19 days, [8-38 days]) and hospital stays (31 days, [15-62 days]); for the non-infected patients, the ICU stay was five days [2-21 days] and the hospital stay was 14.5 days [6-44 days] (p<0.001).
The factors linked to higher mortality rates in the multivariate analysis of the infected patients were ICC functional class III/IV (OR=3.0, 95%CI [1.51 - 5.98], p<0.01), diabetes mellitus (OR=0.48, 95% CI [0.25 - 0.95], p<0.03), cirrhosis (OR=4.62, 95% CI [1.47 - 14.5], p<0.01), male gender (OR=0.68, 95% CI [0.46 - 1.0], p<0.05), mechanical ventilation (OR=1.87, 95% CI [1.19 - 2.95], p<0.01), hemodialysis (OR=1.98, 95% CI [1.05 - 3.75], p<0.03), and the SAPS II score (OR=1.08, 95% CI [1.06 - 1.10], p<0.001) (Table 5).
The present study found a high prevalence (61.6%) of and high mortality rates (37.6%) associated with infection. These numbers are larger than those reported in the literature, which vary from 21.1% to 44.5% for infection prevalence(5,6,11,12) and which are approximately 30% for mortality.(13-17)
International data have shown that infection incidence varies according to the origin of the patient (emergency room, operating room, or infirmary) and the type of ICU. Medical ICU patients have higher incidences of infection (41%), while those destined for elective surgeries have the lowest incidence (12.1%).(11) Despite these findings, the severity and occurrence of organ dysfunction do not depend on these factors.(11,14) These data are consistent with the findings of the present study. The high mortality rate found is also compatible with national and international studies, but recent data show a reduction in this rate from 37% to 30.8% (p < 0.001).(12) There is consistency in the literature, but the reported rates vary between 27 to 50%;(1,5,6,13,17,18) one of the possible explanations for this variation is the variation in the rate of treatment compliance.(12)
The primary site of infection was the lungs (71.2% of the cases), which is consistent with previously published results.(5,6,11,13,14,16,17) In contrast to previous publications,(5,6,11-14,16,17) the urinary tract rather than the abdomen was the second most common site. The literature identifies pulmonary infections, peritonitis, primary bacteremia, and microbiological isolation of gram-positive cocci and gram-negative bacilli as risk factors for increased symptom severity and progression to sepsis.(11,16)
Microbiological isolation was achieved in 50.7% of the infected patients. International data show a wide variation in this rate (from 47.7% to 85.8%).(11,13-15,19) Gram-negative bacilli remain the most commonly isolated organisms in Brazil, but the incidence was higher than has been previously reported (72% vs. 40.1%).(6) Pseudomonas aeruginosa remains the most frequently isolated (24.6%) gram-negative bacillus.(6) The incidence of gram-positive cocci infections in Brazil (33.9%) is increasing, although it is currently lower than the international incidence;(11) however, there is a growing trend toward more gram-negative bacillus infections in the worldwide literature.(15,17)
The infected patients had a significantly higher prevalence of organ dysfunction and higher SOFA scores (Table 3) than did the non-infected patients.(6,12) Higher mortality proportional to the greater organ dysfunction and higher SOFA scores were noted among these patients; the mortality rate in the patients with four or more organ dysfunctions was (65%).(6,11,13) The SAPS II scores were higher in the infected patients (p < 0.001) and were related to higher mortality rates (both ICU and hospital) in these cases.(5,6,8,11,12,14-16,19) The infected patients also had longer ICU and hospital stays.(6,11-13,19) The patients who were already infected at the time of ICU admission or who acquired infection during hospitalization were defined as a high-risk population for mortality.(11)
According to the multivariate analysis (Table 4), increased risk of infection was associated with emergency surgery (p<0.001), mechanical ventilation (p<0.001), and higher SAPS II scores (p<0.001). These results are consistent with those of other publications.(5,6,13,16,20) In contrast to previous reports, comorbidities such as cirrhosis, immunosuppression, ICC functional class III/IV, diabetes mellitus, and solid and hematological malignancies were not directly correlated with infection.(11) One study found that cirrhosis is associated with a greater risk of progression from infection to sepsis.(16)
The difference between the mortality rates of the infected and non-infected patients in the Brazilian intensive care units was statistically significant (37.6% vs. 13.2%, with p<0.001). This result is similar to the findings of previous reports, although the values are lower.(5) The hospital mortality findings were similar (44.2% vs. 17.7%, p<0.001, for the infected and non-infected patients, respectively).(5,13) The factors linked to increased mortality risk among the infected patients in the multivariate analysis were ICC functional class III/IV, diabetes mellitus, cirrhosis, male gender, mechanical ventilation, dialysis, and SAPS II score. There was an association between higher mortality rates and male gender, which is consistent with reports of sex hormones protecting against infection and lowering mortality rates.(21-23) The majority of studies have shown an association between comorbidities and higher mortality rates; not all have found statistically significant associations for previous organ dysfunction, however.(5,6,11-13,16,17,19)
The present study has limitations related to the clear differences in the protocols for approaching ICU infections, which vary with region and the type of hospitals (public or private).
In summary, the present study revealed a higher prevalence of infections in Brazilian ICUs than has been previously reported. There was a clear association between infection and mortality. These findings may be useful for generating institutional health care infection prophylaxis policies and for defining public educational and research priorities in this area.
1. Angus DC, Linde-Zwirble WT, Lidicker J, Clermont G, Carcillo J, Pinsky MR. Epidemiology of severe sepsis in the United States: analysis of incidence, outcome, and associated costs of care. Crit Care Med. 2001;29(7):1303-10. Comment in Wenzel RP, Edmond MB. Severe sepsis-national estimates. Crit Care Med. 2001;29(7):1472-4. [ Links ]
2. Angus DC, Pereira CA, Silva E. Epidemiology of severe sepsis around the world. Endocr Metab Imunne Disord Drug Targets. 2006;6(2):207-12. [ Links ]
3. Vincent JL, Abraham E, Annane D, Bernard G, Rivers E, Van den Berghe G. Reducing mortality in sepsis: new directions. Crit Care. 2002;6 Suppl 3:S1-18. Review. [ Links ]
4. Martin GS, Mannino DM, Eaton S, Moss M. The epidemiology of sepsis in the United States from 1979 through 2000. N Engl J Med. 2003;348(16):1546-54. [ Links ]
5. Silva E, Pedro Mde A, Sogayar AC, Mohovic T, Silva CL, Janiszewski M, Cal RG, de Souza EF, Abe TP, de Andrade J, de Matos JD, Rezende E, Assunção M, Avezum A, Rocha PC, de Matos GF, Bento AM, Corrêa AD, Vieira PC, Knobel E; Brazilian Sepsis Epidemiological Study. Brazilian Sepsis Epidemiological Study (BASES study). Crit Care. 2004;8(4):R251-60. Comment in Linde-Zwirble WT, Angus DC. Severe sepsis epidemiology: sampling, selection, and society. Crit Care. 2004;8(4):222-6. [ Links ]
6. Sales Júnior JAL, David CM, Hatum R, Souza PCSP, Japiassú A, Pinheiro CTS, et al. Sepse Brasil: estudo epidemiológico da sepse em unidades de terapia intensiva brasileiras. Rev Bras Ter Intensiva. 2006;18(1):9-17. [ Links ]
7. Vincent JL, Rello J, Marshall J, Silva E, Anzueto A, Martin CD, Moreno R, Lipman J, Gomersall C, Sakr Y, Reinhart K; EPIC II Group of Investigators. International study of the prevalence and outcomes of infection in intensive care units. JAMA. 2009;302(21):2323-9. Comment in Opal SM, Calandra T. Antibiotic usage and resistance: gaining or losing ground on infections in critically ill patients? JAMA. 2009;302(21):2367-8. [ Links ]
8. Le Gall JR, Lemeshow S, Saulnier F. A new Simplified Acute Physiology Score (SAPS II) based on a European/North American multicenter study. JAMA. 1993;270(24):2957-63.Erratum in: JAMA 1994 May 4;271(17):1321. [ Links ]
9. Vincent JL, Moreno R, Takala J, Willatts S, De Mendonça A, Bruining H, et al. The SOFA (Sepsis-related Organ Failure Assessment) scoreto describe organ dysfunction/failure. On behalf of the Working Group on Sepsis-Related Problems of the European Society of Intensive Care Medicine. Intensive Care Med. 1996;22(7):707-10. [ Links ]
10. Calandra T, Cohen J; International Sepsis Forum Definition of Infection in the ICU Consensus Conference. The international sepsis forum consensus conference on definitions of infection in the intensive care unit. Crit Care Med. 2005;33(7):1538-48. [ Links ]
11. Alberti C, Brun-Buisson C, Burchardi H, Martin C, Goodman S, Artigas A, et al. Epidemiology of sepsis and infection in ICU patients from an international multicentre cohort study. Intensive Care Med. 2002;28(2):108-21. Erratum in Intensive Care Med. 2002;28(4):525-6. [ Links ]
12. Alberti C, Brun-Buisson C, Chevret S, Antonelli M,Goodman SV, Martin C, Moreno R, Ochagavia AR, Palazzo M, Werdan K, Le Gall JR; European Sepsis Study Group. Systemic inflammatory response and progression to severe sepsis in critically ill infected patient. Am J Respir Crit Care Med. 2005;171(5):461-8. [ Links ]
13. Molina FJ, Díaz CA, Barrera L, De La Rosa G, Dennis R, Dueñas C, et al. [Microbiological profile of infections in the Intensive Care Units of Colombia (EPISEPSIS Colombia)]. Med Intensiva. 2011;35(2):75-83. Spanish. [ Links ]
14. Vincent JL, Sakr Y, Sprung CL, Ranieri VM, Reinhart K, Gerlach H, Moreno R, Carlet J, Le Gall JR, Payen D; Sepsis Occurrence in Acutely Ill Patients Investigators. Sepsis in European intensive care units: results of the SOAP study. Crit Care Med. 2006;34(2):344-53. Comment in Lee WL, Ferguson ND.SOAP and sepsis--analyzing what comes out in the wash. Crit Care Med. 2006;34(2):552-4. [ Links ]
15. Luzzaro F, Ortisi G, Larosa M, Drago M, Brigante G, Gesu G. Prevalence and epidemiology of microbial pathogens causing bloodstream infections: results of the OASIS multicenter study. Diagn Microbiol Infect Dis. 2011;69(4):363-9. [ Links ]
16. Levy MM, Dellinger RP, Townsend SR, Linde-Zwirble WT, Marshall JC, Bion J, et al. The Surviving Sepsis Campain: results of an international guideline-based performance improvement program targeting severe sepsis. Intensive Care Med. 2010;36(2):222-31. Review. [ Links ]
17. Zahar JR, Timsit JF, Garrouste-Orgeas M, Français A, Vesim A, Descorps-Declere A, et al. Outcomes in severe sepsis and patients with septic shock: pathogen species and infection sites are not associated with mortality. Crit Care Med. 2011;39(8):1886-95. Erratum in Crit Care Med. 2011;39(10):2392. Vesim, Aurélien [corrected to Vesin, Aurélien]. Comment in Mergulhão P, Paiva JA. The importance of source identification in septic patients. Crit Care Med. 201139(12):2786-7; author reply 2787. Cohen J. A role for the micro-organism in the outcome from infection? A principle challenged. Crit Care Med. 2011;39(8):2001-2. Rubulotta F, Ramsay G. Outcomes in severe sepsis and patients with septic shock do not matter! PIRO is a score to treat severe septic and septic shock patients not to measure outcomes. Crit Care Med. 2012;40(2):706-7; author reply 707-8. [ Links ]
18. Lesur O, Roussy JF, Chagnon F, Gallo-Payet N, Dumaine R, Sarret P, et al. Proven infection-related sepsis induces a differential stress response early after ICU admission. Crit Care. 2010;14(4):R131.Comment in Peng J, Du B. Sepsis-related stress response: known knowns, known unknowns, and unknown unknowns. Crit Care. 2010;14(4):179. [ Links ]
19. Azoulay E, Alberti C, Legendre I, Buisson CB, Le Gall JR; European Sepsis Group. Post-ICU mortality in critically ill infected patients: an international study. Intensive Care Med. 2005;31(1):56-63. Erratum in Intensive Care Med. 2005;31(2):318-20. [ Links ]
20. Lambert ML, Suetens C, Savey A, Palomar M, Hiesmayr M, Morales I, et al. Clinical outcomes of health-care-associated infections and antimicrobial resistence in patients admitted to European intensive-care units: a cohort study. Lancet Infect Dis. 2011;11(1):30-8. Comment in Grundmann H, de Kraker M, Davey P. Clinical impact of antimicrobial resistance: design matters. Lancet Infect Dis. 2011;11(5):344; author reply 344-5. Vincent JL. Does microbial resistance matter? Lancet Infect Dis. 2011;11(1):3-4. [ Links ]
21. Oberholzer A, Keel M, Zellweger R, Steckholzer U, Trentz O, Ertel W. Incidence of septic complications and multiple organ failure in severely injured patients in sex epecific. J Trauma. 2000;48(5):932-7. [ Links ]
22. Raju R, Chaudry IH. Sex steroids/receptor antagonist: their use as adjuncts after trauma-hemorrhage for improving immune/cardiovascular responses and for decreasing mortality from subsequent sepsis. Anesth Analg. 2008;107(1):159-66. [ Links ]
23. Angstwurm MW, Gaertner R, Schopohl J. Outcome in elderly patients with severe infection is influenced by sex hormones but not gender. Crit Care Med. 2005;33(12):2786-93. [ Links ]
Corresponding author: Submitted on April 11, 2012 Conflicts of interest: None.
Avenida Albert Einstein, 627 - 5th Floor - Morumbi
Zip Code: 05651-901 - São Paulo (SP), Brazil
Accepted on June 22, 2012
Submitted on April 11, 2012
Conflicts of interest: None.