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Ciência Rural

Print version ISSN 0103-8478

Cienc. Rural vol.44 no.7 Santa Maria July 2014

https://doi.org/10.1590/0103-8478cr20131006 

Pathology

Exogenous ATP administration prevents ischemia/reperfusion-induced oxidative stress and tissue injury by modulation of hypoxanthine metabolic pathway in rat ovary

Administração exógena de ATP impede isquemia/induzidas por reperfusão de estresse oxidativo de tecidos e lesões pela modulação da hipoxantina via metabólica em ovários de ratos

Serkan Kumbasar I  

Nihal Cetin II  

Omer Erkan Yapca III  

Ebru Sener IV  

Unal Isaoglu V  

ehmet Yilmaz III  

Suleyman Salman VI  

Ayse Nur Aksoy V  

Mehmet Ali Gul VII  

Halis Suleyman II   * 

(I)Department of Obstetrics and Gynecology, Faculty of Medicine, Sakarya University, Sakarya, Turkey.

(II)Department of Pharmacology, Faculty of Medicine, Recep Tayyip Erdogan University, 53000, Rize, Turkey, E-mail: halis.suleyman@gmail.com.

(III)Department of Obstetrics and Gynecology, Faculty of Medicine, Ataturk University, Erzurum, Turkey.

(IV)Department of Pathology, Erzurum Region Education and Research Hospital, Erzurum, Turkey.

(V)Department of Obstetrics and Gynecology, Nene Hatun Obstetrics and Gynecology Hospital, Erzurum, Turkey.

(VI)Department of Obstetrics and Gynecology, Taksim Region Education and Research Hospital, Istanbul, Turkey.

(VII)Department of Biochemistry Ataturk University, Faculty of Medicine, Erzurum-Turkey.


ABSTRACT

In this study, xanthine oxidase (XO), malondialdehyde (MDA), myeloperoxidase (MPO) and glutathione (GSH) levels in the ovarian tissues of rats during the development of ischemia and postischemia-induced reperfusion were investigated, and the effect of ATP on ischemia-reperfusion (I/R) damage was biochemically and histopathologically examined. The results of the biochemical analyses demonstrated that ATP significantly reduced the level of XO and MDA and increased the amount of GSH in both ischemia and I/R-applied ovarian tissue at the doses administered. Furthermore, ATP significantly suppressed the increase in MPO activity that occurred following the application of post ischemia reperfusion in the ovarian tissue. The biochemical results obtained in the present study coincide with the histological findings. The severity of the pathological findings, such as dilatation, congestion, haemorrhage, oedema and polymorphonuclear nuclear leukocytes (PMNLs), increased in parallel with the increase observed in the products of XO metabolism. In conclusion, exogenously applied ATP prevented I/R damage by reducing the formation of XO in ischemic ovarian tissue.

Key words: xanthine oxidase metabolism; ischemia/reperfusion injury; antioxidant; rat

RESUMO

Neste estudo, a xantina oxidase (XO), o malondialdeído (MDA), mieloperoxidase ( MPO ) e glutationa ( GSH) nos tecidos do ovário de ratos, durante o desenvolvimento de isquemia e reperfusão induzida por pós-isquemia foi investigada, e o efeito de ATP em isquemia e reperfusão (I/R). O dano foi verificado por provas bioquímicas e por histopatologia. Os resultados das análises bioquímicas mostraram que o ATP reduziu significativamente o nível de XO e MDA e aumentou a quantidade de GSH em ambas as isquemia e no tecido do ovário de I / R - aplicado nas doses administradas. Além disso, o ATP suprimiu significativamente o aumento na atividade de MPO que ocorreu na sequência da aplicação de pós-isquemia reperfusão no tecido ovariano. Os resultados bioquímicos obtidos no presente estudo coincidem com os achados histológicos. A gravidade dos achados patológicos, como a dilatação, congestão, hemorragia, edema e polimorfonucleares leucócitos nucleares (PMNLs), aumentou em paralelo com o aumento observado nos produtos do metabolismo XO. Em conclusão, aplicando exogenamente ATP impedido de I/R, houve danos pela redução da formação de tecido de ovário de XO na isquemia.

Palavras-Chave: metabolismo oxidativo da xantina; isquemia/reperfusão; antioxidante; rato

INTRODUCTION

Ischemia is defined as partial or complete oxygen (O2) deprivation in tissues due to reduced or completely interrupted blood flow. In ischemia, the synthesis of high-energy phosphates, such as ATP, is decreased in cells due to interruption of the O2 flow into the tissue (JENNINGS & REIMER, 1991; REITER, 1995). The decrease in ATP leads to inhibition of the Na+, K+ ATPase pump in the cell membrane and an increase in intracellular Na+ and Ca2+ concentrations (GREEN et al., 1989). Increased intracellular Ca2+ concentrations are reported to be cytotoxic (ORRENIUS et al., 1992). ATP, adenosine mono-phosphate (AMP), adenosine, inosine and hypoxanthine are produced from ATP, and ATP utilisation continues during the ischemia period. Furthermore, xanthine dehydrogenase (XD) is converted to xanthine oxidase (XO) in this period. In healthy tissues, hypoxanthine is metabolised by XD, whereas it is metabolised by XO in ischemic tissue (PARKS et al., 1988). Toxic O2 radicals are not formed in healthy tissues because nicotinamide dinucleotide is used in the metabolism of hypoxanthine by XD. Toxic O2 radicals are produced as intermediates in ischemic tissues because O2 is used in the metabolism of hypoxanthine by XO (GRACE, 1994; PARKS et al., 1988). Hypoxanthine cannot be metabolised by XO unless reperfusion occurs in ischemic tissue. As is known, the emptying of cellular energy stores may lead to cell death in long-lasting ischemia (PARKS et al., 1988; ZIMMERMAN & GRANGER, 1992). Therefore, the intervention in ischemic conditions has always been adequate perfusion of the tissue. During reperfusion, XO produced during ischemia converts hypoxanthine to xanthine using locally available O2, leading to excessive free O2 radical production and a decrease in antioxidant defence mechanisms (LINDSAY et al., 1990; LI & JACKSON, 2002). Previous studies showed that XO, the production of which increases during ischemia due to ATP deficiency, is an essential component in the mechanism of reperfusion injury. This indicates that ATP administration may be beneficial for the prevention of ischemia/reperfusion (I/R) injury.

Thus far, no studies have investigated the potentially protective effect of exogenous administration of ATP on I/R-induced ovarian injury in rats. Therefore, the aim of the present study was to investigate the effect of exogenous administration of ATP prior to experimentally induced ischemia and I/R of the rat ovary on biochemical variables and histopathological parameters.

MATERIAL AND METHODS

Animals

A total of 42 female adult Albino Wistar rats weighing between 220-235g were used for the experiment. The rats were obtained from Ataturk University Medical Experiments Research and Training Center (Erzurum, Turkey). The protocols and the procedures were approved by the local Animal Experimentation Ethics Committee (Date: 24. 04. 2012, meeting no: B.30.2.ATA.0.01.02/1845).

Chemical substances

Thiopental sodium was provided by IE Ulagay-Turkey and ATP was supplied by Zdorove Narodu Ukraine.

General procedure

The animals were allocated to 7 groups as Ovarian Ischemia (OI), Ovarian Ischemia/Reperfusion (OIR), 2mg kg-1 ATP + Ovarian Ischemia (AOI-2), 4mg kg-1 ATP + Ovarian Ischemia (AOI-4), 2mg kg-1 ATP+ Ovarian Ischemia/Reperfusion (AOIR-2), 4mg kg-1 ATP+ Ovarian Ischemia/Reperfusion (AOIR-4), and Control Group (Sham Operated- Healthy Control Group (CG). Surgical procedures were performed under sterile conditions in a suitable laboratory environment by administering 25mg kg-1 intraperitoneal (IP) thiopental sodium anesthesia.

Surgical and pharmacological procedures

ATP was injected into AOI-2, AOI-4, AOIR-2 and AOIR-4 groups via the intraperitoneal route 1h before anaesthesia. A 3h ischemia period was then simulated through vascular clamping to the lower part of the right ovaries of rats assigned to OI, OIR, AOI-2, AOI-4, AOIR-2 and AOIR-4 groups. At the end of this period, the ovaries of the rats in the OI, AOI-2, AOI-4 groups were removed. Reperfusion was provided for 2h by removing the vascular clamp in the OIR, AOIR-2 and AOIR-4 groups. The ovaries of the rats in the OIR, AOIR-2 and AOIR-4 groups were removed. Histopathological and biochemical analyses were performed subsequently in all the samples (KURT et al., 2013). A 2-2.5cm long vertical incision was made in the lower abdomen of each rat to reach the ovaries. Ischemia was not performed in the ovaries of the rats in CG. The ovarian tissue samples were collected from all the experimental groups, and the assay was conducted simultaneously.

Biochemical procedures

Biochemical analysis of ovarian tissue

The homogenates were prepared to measure the enzymatic activity in the ovarian tissues. The XO, malondialdehyde (MDA), myeloperoxidase (MPO) and total glutathione (tGSH) enzyme activities in the supernatants obtained from these homogenates were determined using established methods reported in the literature.

Determination of xanthine oxidase (XO) activity

The XO activity was measured spectrophotometrically by the formation of uric acid from xanthine through the increase in absorbance at 293nm. In this method, phosphate buffer with EDTA (pH 7.5) is used as the solvent that reaction takes place. A total of 50µl of the sample and 50µl of xanthine (4mM) were added to the phosphate buffer, and then the mixture was incubated for 30min at 37°. A total of 100% (w/v) of TCA was added to stop the reaction mixture. The mixture was centrifuged at 4500g for 20min. The increase in the absorbance of the supernatant was then measured at 293nm (ROWE et al., 1966).

Determination of malondialdehyde (MDA) activity

The concentrations of ovarian lipid peroxidation were determined by estimating MDA activity using the thiobarbituric acid test (OHKAWA et al., 1979). The rat ovaries were promptly excised and rinsed with cold saline. The ovarian tissue, weighed and homogenised in 10ml of 100g l-1 KCl. The homogenate (0.5ml) was added to a solution containing 0.2ml of 80g l-1 sodium lauryl sulfate, 1.5ml of 200g l-1 acetic acid, 1.5ml of 8g l-1 2-thiobarbiturate and 0.3ml of distilled water. The mixture was incubated at 98°C for 1h. Upon cooling, 5ml of n-butanol:pyridine (15:l) were added. The mixture was vortexed for 1min and centrifuged for 30min at 4000rpm. The absorbance of the supernatant was measured at 532nm. The standard curve was obtained by using 1,1,3,3-tetramethoxypropane.

Determination of myeloperoxidase (MPO) activity

The activity of MPO in the total homogenate was measured according to the method of Wei and Frenkel, with some modifications (WEI & FRENKEL, 1991). The sample was weighed and homogenised in a 2ml of 50mM phosphate buffer containing 0.5% hexadexyltrimethyl ammonium bromide (HDTMAB) and centrifuged at 3500rpm for 60min at 4°C. The MPO activity in the supernatant was determined using 1,3ml 4-aminoantipyrine-2% phenol (25mM) solution. A total of 25mM 4-aminoantipyrine-2% phenol solution and 0.0005% 1.5ml H2O2 were added and equilibrated for 3-4min. After establishing the basal rate, 0.2ml of sample suspension was added and quickly mixed. Increases in the absorbance at 510nm were recorded at 0.1min intervals for 4min. The absorbance was measured at 412nm using a spectrophotometer.

Determination of total glutathione (tGSH)

The amount of tGSH in the total homogenate was measured according to the method of Sedlak and Lindsay with some modifications (SEDLAK & LINDSAY, 1968). The sample was weighed and homogenised in 2ml of 50mM Tris-HCl buffer containing 20mM of EDTA and 0.2mM of sucrose at pH 7.5. The homogenate was immediately precipitated with 0.1ml of 25% trichloroacetic acid, and the precipitate was removed after centrifugation at 4200rpm for 40min at 4°C. The GSH level of the supernatant was then determined. A total of 1500µl of measurement buffer (200mMTris-HCl buffer containing 0.2mM of EDTA at pH 7.5), 500µl of supernatant, 100µl of 5,5-dithiobis (2-nitrobenzoic acid) (DTNB) (10mM) and 7900µl of methanol were added to a tube, vortexed and incubated for 30min at 37°C. DTNB was used as a chromogen, and it formed a yellow-coloured complex with SH groups. The absorbance was measured at 412nm using a spectrophotometer. The standard curve was obtained using reduced glutathione.

Histopathological procedures

The ovaries that were removed from the rats were fixed in 10% formalin solution, sections 4µm in thickness were obtained from the paraffin blocks obtained after the routine tissue follow-up process, and the sections were stained with hematoxylin&eosin (H&E) stain after deparaffinization and rehydration. All the sections were encoded and examined under light microscopy (Olympus CX 51, Tokyo, Japan).

Statistical analysis

All data were subjected to a one-way analysis of variance (ANOVA) using Statistical Package for the Social Sciences (SPSS) 18.0 software. Multiple comparisons were analysed using the Duncan's multiple range test. A P-value 0.05 was considered statistically significant. The results are presented as the mean  standard error of mean (SEM).

RESULTS

Biochemical results

As seen in table 1, XO activity in the ovarian tissue of the ischemia-induced OI group was significantly higher than in the OIR group (<0.0001). In addition, the levels of MDA and MPO in the OIR group in which XO activity was lower increased in comparison to the OI group, and the amount of tGSH significantly decreased (P<0.0001). ATP administered in 2 and 4mg kg-1 doses significantly prevented increases in XO, MDA and MPO levels during ischemia and postischemia-induced reperfusion in ovarian tissue and decreases in tGSH. No significant change was observed in the MPO activity of the ischemic ovarian tissue.

Table 1 - The effects of ATP on biochemical parameters 

Groups XO U g-1 protein P MDA µmol g-1 protein P MPO U g-1 protein P tGSH nmol g-1 protein P
OI 33.2 ± 3.2 f <0.001 5.8 ± 0.1 d <0.0001 4.6 ± 0.65 a <0.0001 5.5 ± 1.1 b <0.0001
OIR 20.2 ± 2.7 e - 11.3 ± 0.22 e - 19.7 ± 2.1 c - 2.6 ± 0.87 a -
AOI-2 12.8 ± 1.4 d <0.001 3.8 ± 0.09 abc <0.0001 4.9 ± 0.88 a <0.0001 7.4 ± 1.8 c <0.0001
AOI-4 9.2 ± 1.6 c <0.0001 3.0 ± 0.08 ab <0.0001 5.1 ± 0.74 a <0.0001 8.3 ± 2.1 c <0.0001
AOIR-2 7.2 ± 1.5 bc <0.0001 4.6 ± 0.11 c <0.0001 13.3 ± 2.6 b <0.05 6.7 ± 1.9 bc <0.0001
AOIR-4 5.2 ± 1.3 ab <0.0001 4.1 ± 0.09 bc <0.0001 11.5 ± 2.9 b <0.05 7.8 ± 1.6 c <0.0001
CG 3.9 ± 0.87 a <0.0001 2.7 ± 0.04 a <0.0001 4.2 ± 0.79 a <0.0001 9.7 ± 2.2 bc <0.0001

The effects of ATP on the XO, MDA, MPO and tGSH levels in rat ovarian tissue ischemia /reperfusion. OI: Ovarian Ischemia, OIR: Ovarian Ischemia /Reperfusion, AOI-2: 2mg kg-1 + ATP Ovarian Ischemia, AOI-4: 4mg kg-1 + ATP Ovarian Ischemia, AOIR-2: 2mg kg-1 + ATP Ovarian Ischemia/ Reperfusion, AOIR-4: 4mg kg-1 + ATP Ovarian Ischemia/ Reperfusion, CG: Healthy Control Group. Results are the means ± Standart error of the mean.(Duncan's multiple range test) (N=6).

Histopathological result

A normal histopathological appearance is observed in figure 1.1.A-B. A moderate dilation, congestion (arrow) and mild hemorrhage (star) were observed in the vessels of the ipsilateral ovarian stroma in the OI group (Figure 1.2A.) compared to a normal histopathological ovary in control group. High magnification assessment provide a better insight in figure 1.2B. In figure 1.3A, vasodilation and congestion (arrow), edema, permanent and severe hemorrhage (star) were observed in the low power field; in Figure. 1.3B, migration and adhesion of a large number of PMNLs (double arrow) to the endothelium were observed in the high power field in the OIR group.

Figure 1 Light microscopy photograph of the ovary in CG. A normal histopathological appearance was observed figure 1.1A High magnification of figure 1.1B. Light microscopy photographs of the ipsilateral ovary in the OI group. A moderate dilation and congestion (arrow) and mild hemorrhage (star) were observed in the vessels of the ovarian stroma figure 1.2A. High magnification of figure 1.2B. Light microscopy photographs of the ipsilateral ovary in the OIR group. In figure 1.3A, vasodilation and congestion (arrow), edema, permanent and severe hemorrhage (star) were observed in the low power field; in 3B, migration and adhesion of a large number of PMNLs (double arrow) to the endothelium were observed in the high power field. Light microscopy photographs of ipsilateral ovaries in the AOI-2 group. A mild congestion (arrow) and mild stromal hemorrhage were observed in figure 1.4A. High magnification of figure 1.4B. In Figure 1.5A light microscopy photographs of the ipsilateral ovaries in the AOI-4 group. High magnification of figure 1.5B. A near-normal histomorphological appearance was observed. In 1.6 light microscopy photographs of the ipsilateral ovaries in the AOIR-2 group. While moderate congestion and dilation (arrow), moderate stromal hemorrhage and edema (star) were observed in the low power field in 6A, migration and adhesion of PMNLs to the endothelium (arrow) were observed in 6B. In figure 1.7A light microscopy photographs of the ipsilateral ovaries in the AOIR-4 group. Mild hemorrhage (arrow) in the ovarian stroma, mild vascular congestion (star) and minimal edema were observed. High magnification of figure 1.7B 

A mild congestion (arrow) and mild stromal hemorrhage were observed in figure 1.4A for the AOI-2 group. High magnification assessment provide a better insight in figure 1.4B. In figure 1.5A., a near-normal histomorphological appearance was observed in the AOI-4 group. High magnification assessment provide a better insight in figure 1.5B.In the AOIR-2 group, moderate congestion, dilation (arrow), moderate stromal hemorrhage and edema (star) were observed in the low power field in figure. 1.6A, while migration and adhesion of PMNLs to the endothelium (arrow) were observed in figure. 1.6B. As seen in figure 2.7. mild hemorrhage (arrow) in the ovarian stroma, mild vascular congestion (star) and minimal edema were observed in the AOIR-4 group (Table 2). High magnification assessment provide a better insight in figure 1.7B.

Table 2 - The histopathological results of ATP 

Congestion Hemorrhage Dilation Edema Migration and adhesion of PMNLs
CG - - - - -
OI ** * ** - -
OIR *** *** *** *** ***
AOI-2 * * - - -
AOI-4 - * - - -
AOIR-2 ** ** ** ** **
AOIR-4 * * - - -

The histopathological results in protective effect of ATP exogenous administration on I/R-induced ovarian injuries in rats. *refers: mild, ** refers: moderate, *** refers: severe damage

DISCUSSION AND CONCLUSION

This study investigated the effect of ATP on biochemical and histopathological damage caused by I/R. The results of the biochemical tests demonstrated that XO activity in the ischemia-induced OI group was 1.6 times higher than in the OIR group. In the literature, it has been reported that the presence of higher XO activity in ischemic tissue is due to the conversion of the xanthine dehydrogenase enzyme into XO by a calcium-mediated protease catalyst in the ischemia mediator (GRACE, 1994). In addition, the reduced occurrence of XO activity in the OIR group in comparison to the OI group could possibly be attributed to the XO activity being consumed in the production of XO oxidant. ATP used in doses of 2 and 4mg kg-1 in this study significantly decreased XO activity during the formation of ischemia and postischemia-induced reperfusion in the ovarian tissue. The oxidation of sulfhydryl groups within XD molecules of cells leads to the formation of XO (HARRISON, 2002). Thus, the decrease in the formation of XO in the ovarian tissue of the rat groups that received ATP exogenously may be due to antioxidant activity associated with the generation of ATP. Similarly, the levels of oxidants, such as MDA and MPO, in the ovarian tissue of the OIR group significantly increased in comparison to those in the OI group, and their tGSH levels decreased. Paller M.S. demonstrated that free radicals develop at a low rate during ischemia but that they are produced in greater numbers when re-oxygenation of the tissue by reperfusion takes place (PALLER, 1994). Another study also reported that while XO accumulates during the ischemia period, a large number of toxic radicals are produced by transforming hypoxanthine into xanthine using the O2 introduced by reperfusion, (CARDEN & GRANGER, 2000). In common with these studies, Ingec et al. reported that the amount of oxidant increased only in ischemia-induced ovarian tissue and that it was greatly intensified by reperfusion (INGEC et al. 2011). As is well-known, AMP produced by aerobic tissues in the aerobic tissues provides the energy required to maintain cell viability by being broken down into adenosine, inosine and hypoxanthine. In ischemia, the consumption of ATP increases, and its production decreases (JENNINGS & REIMER, 1991). This event leads to the depletion of energy stores and the inhibition of the Na+, K+-ATPase pump found in the cell membrane. ATPase pump inhibition causes an excessive increase in intracellular Na+ and Ca2+ ion concentrations and leads to cell damage (GREEN et al., 1989). The prevention of ischemia and reperfusion damage by the administration of ATP in the experiment coincides with the information reported in the literature.

The use of ATP in doses of 2 and 4mg kg-1 significantly prevented the rise of MDA and the reduction of tGSH in ischemic ovarian tissue. At the same doses, ATP significantly suppressed the levels of MDA and MPO, both of which had increased as a result of reperfusion. Free O2 radicals, which are known to mediate reperfusion, result in the release of toxic products, such as aldehydes and MDA, from lipids and stimulate oxidation in the cell membrane (GOULART et al., 2005). MDA, as the final product of lipid peroxidation, causes severe damage to the cell membrane and its intracellular structures (GOULART et al., 2005). The high level of MPO in reperfusion could possibly originate from the release of MPO by large numbers of polymorphonuclear nuclear leukocytes (PMNLs) in the blood entering the ischemic tissue during reperfusion (ZIMMERMAN & GRANGER, 1992). PNLs convert molecular O2 into superoxide and hydrogen peroxide. The hydrogen peroxide in the chloride ions is reduced to hypochlorous acid, a toxic and strong oxidant, via the MPO enzyme found in the azurophilic granules of neutrophils (ZIMMERMAN & GRANGER, 1992). Studies have experimentally demonstrated that postischemia reperfusion leads to severe damage of other tissues, as well as the ovaries (BOREKCI et al., 2009; COSKUN et al., 2011; TOK et al., 2012). Moreover, previous studies reported that levels of ATP and Na+-K+-ATPase are decreased in ischemic tissue (GABRYEL et al., 2012; SINGH et al., 2012). The biochemical results obtained in the present study coincide with the histopathological findings. In addition, the severity of the pathological findings, such as dilatation, congestion, haemorrhage, oedema and PNLs, increased in parallel with the rise in XO metabolism products. In conclusion, exogenously administered ATP prevented the increase of XO, MDA and MPO, all of which are known to be oxidant parameters in ovarian tissue in ischemia and postischemia-induced reperfusion. These results suggest that the use of ATP at the clinic would be helpful as a preventive agent of both ovarian ischemia and post ischemia reperfusion damage.

ACKNOWLEDGEMENTS

Authors do not have financial support.

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Received: July 29, 2013; Accepted: January 10, 2014

*Autor para correspondência.

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