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Ciência Rural

Print version ISSN 0103-8478On-line version ISSN 1678-4596

Cienc. Rural vol.45 no.8 Santa Maria Aug. 2015  Epub May 12, 2015

http://dx.doi.org/10.1590/0103-8478cr20141225 

Ciência do Solo

Leaf water potential, nutritional status and must composition in grapes 'Pinot Nero' with and without irrigation

Potencial de água em folhas, estado nutricional e composição do mosto em viníferas 'Pinot Nero' com e sem irrigação

Marlise Nara Ciotta*  1  

Carlos Alberto Ceretta 2  

Massimo Tagliavini 3  

Damiano Zanotelli 3  

Damiano Moser 3  

Gilberto Nava 4  

Duilio Porro 5  

Gustavo Brunetto 2  

IPrograma de Pós-graduação em Ciência do Solo, Universidade Federal de Santa Maria (UFSM), 97105-900, Santa Maria, RS, Brasil.

2Departamento de Solos, UFSM, Santa Maria, RS, Brasil.

3Libera Università di Bolzano, Bolzano, Itália.

4Embrapa Clima Temperado, Pelotas, RS, Brazil.

5Fondazione Mach san Michele all'Adige, Trento, Itália.

ABSTRACT

Irrigating vineyard soils can affect grapevine water potential, nutritional status, and must composition. This study aimed to evaluate leaf water potential, nutritional status, and must composition in cv. 'Pinot Nero' grapevines grown with and without irrigation. The experiment was conducted at a commercial vineyard of 'Pinot Nero' 828 grafted on SO4 rootstock, established in 2002 in Trento, Northern Italy. The treatments were irrigated (I) and non-irrigated (NI) throughout the 2013 crop season. The criteria evaluated were the water potential of the leaves, total nutrient content in the leaves and berries, and weight of 100 berries, as well as the total soluble solids content, pH, and total titratable acidity of the must. Despite providing a less negative water potential for the grapevine leaves, irrigation did not affect the nutritional status or must composition, and it only slightly interfered with berry nutrient content.

Key words: nutrients; irrigation; Vitis vinifera.

RESUMO

A irrigação em solos de vinhedos pode afetar o potencial hídrico da videira, o estado nutricional e a composição do mosto. O trabalho objetivou avaliar o potencial de água em folhas, o estado nutricional e a composição do mosto, em videiras da cv. 'Pinot Nero', cultivadas com e sem irrigação. O experimento foi conduzido em um vinhedo comercial de 'Pinot Nero' 828, enxertada sobre o porta enxerto SO4, implantado em 2002 em Trento, Norte da Itália. Os tratamentos foram com irrigação (I) e sem irrigação (SI) ao longo da safra de 2013. Avaliou-se o potencial hídrico das folhas, o teor total de nutrientes em folhas e bagas, a massa de 100 bagas e, no mosto, foram avaliados o teor de sólidos solúveis totais, pH e acidez total titulável. A irrigação, apesar de proporcionar potencial de água menos negativo nas folhas da videira, não afetou o estado nutricional, a composição do mosto e pouco interferiu no teor de nutrientes na baga.

Palavras-Chave: nutrientes; irrigação; Vitis vinifera.

In northern Italy, grapevines (Vitis vinifera L.) such as cv. 'Pinot Nero' are irrigated during plant growth, mainly during berry growth and ripening, which usually occur between August and September, when there is little rainfall (MARIANI et al., 2012). However, the impact of providing water to the soil on nutritional status and must composition of the grapevines, which affects wine quality, is poorly understood for this region. In irrigated vineyard soils, water availability in the soil is expected to favor adequate water uptake by grapevines, thus resulting in higher water potential in the leaves compared to plants in water-limited soil. In addition, grapevines in irrigated soils show higher uptake and transport of nutrients, such as nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), and magnesium (Mg), which preferentially accumulate in organs undergoing increased cell division, such as leaves and growing berries. However, if irrigated grapevines absorb high levels of certain nutrients, such as N, increased plant growth would be expected, and thus, a portion of the nutrients stored inside the berries may be reallocated to the growing organs. Furthermore, increased soil water availability due to irrigation can increase the moisture content inside plants, including inside the berries, thus increasing their diameter and weight. This phenomenon promotes sugar dilution, estimated by the total soluble solids (TSS) content, and increases the dilution of acids, especially malic acid, as estimated by total titratable acidity (CONDE et al., 2007). This study aimed to evaluate leaf water potential, nutritional status, and must composition in cv. 'Pinot Nero' grapevines grown with and without irrigation.

The experiment was conducted at a commercial vineyard of cv. 'Pinot Nero' 828, grafted on SO4 rootstock, established in 2002 in a Leptosol soil, in Trento, Northern Italy. Before establishing the experiment, the soil was characterized by 240, 520, and 240g kg-1 of clay, silt, and sand, respectively; pH in water of 7.85; 2.4g of total N kg-1, 289mg kg-1 of exchangeable K and 15mg kg-1of available P; 13.6cmolc of Ca kg-1; and 2.1cmolc of kg-1 Mg. In June 2013, randomized block design experiment was established, with five replicates of five plants each. The treatments were irrigated (I) and non-irrigated (NI). Leaf water potential (ψ) was measured using a Scholander pressure chamber (VAN LEEUWEN et al., 2009), in July (3rd, 17th, and 26th) and August (2nd, 14th, 23rd, and 28th). The readings were taken from two fully expanded complete leaves per plant on two branches located in the middle portion, on all of the sides of the plant. Leaves and berries were collected in three times, August, September and October. Five leaves were collected at the middle portion of each branch, selected from both sides of the plant. The leaves were dried in a forced-air oven at 65°C, ground, and digested with HNO3, in a microwave, after which they were analyzed for total levels of N, P, Ca, Mg, and K using inductively coupled plasma- and optical emission spectrometry (ICP-)OES). During the same leaf collection periods, 150 berries per plant were collected from the upper, middle, and lower portions of each grape cluster. The weight of 100 berries was determined using a digital scale. Then, the berries were crushed, and the total levels of N, P, Ca, Mg, and K were determined from a portion of the must using ICP-OES, after digestion with HNO3 in a microwave; a second portion of the must was used to evaluate total soluble solids (SST), pH, and total titratable acidity. The results obtained were subjected to analysis of variance (ANOVA); when significant, the means were compared by Tukey's comparison test at 5% probability

The leaf water potential (ψ) did not differ significantly at the first two assessments, July 3rd and 17th, between the I and NI plants (Figure 1). Between July 17th and August 23rd, ψ was more negative in leaves of the NI plants, with the lowest ψ, -0.5 MPa for NI and -0.2 MPa for I. However, starting on August 28th, when rainfall of approximately 20mm was observed and the soil moisture content likely increased in NI treatment, the leaf ψ values did not statistically differ between the I and NI plants. The more negative ψ values observed from July 17th until August 28thin leaves of the NI plants were likely due to the higher mean air temperature (mean of 22.3ºC) and lower relative humidity (69.29 and 62.90%, respectively) in July and August, which promoted high vapor pressure deficits (8.52 and 10.51 hPa, respectively). Thus, lower water availability in the soil may have led to lower water uptake by plants, thus making ψ in the plants more negative. The total levels of K, N, P, Ca, and Mg in the complete leaves were unchanged by irrigation in August, September, and October (Table 1), possibly because although the rainfall volume was not high in August, it may have been sufficient to maintain water availability in the NI soil, allowing for nutrient supply and uptake. Similarly, even without irrigation, adequately distributed rainfall of 69mm in September and 127mm in October was most likely sufficient to maintain soil moisture at the desired levels, thus favoring nutrient uptake by the grapevines. This is confirmed by the levels of N, P, K, Ca, and Mg in the leaves at the three collection periods (Table 1). Irrigation did not alter the total levels of P, K, and Mg in the berries (Table 1), which may be explained by the characteristics and way these nutrients accumulate in plants, especially in the grape clusters. During cluster development, the entry of water and nutrients via the xylem and phloem is the main mechanism of increasing volume and growth. After the berries change color, when the clusters ripen, certain nutrients (including P, K, and Mg) continue to accumulate in the clusters via phloem. This is one reason why K is usually present at higher levels in clusters at harvest. Additionally, the total Ca content was higher in the berries of non-irrigated plants, which may be partially explained by the possible lower water availability in the soil, as indicated by the negative ψ values in the complete leaves (Figure 1). However, the Ca content absorbed was sufficient and increased concentration on the berries. But as the levels were high there was no change in the levels in the leaves. During August and September, the total N levels in the berries were higher in the non-irrigated plants. This most likely occurred because the water content and the levels of mineral N forms available in the soil, especially of nitrate (N-NO3 -), were adequate to meet the grapevines requirements, even in the NI treatment. In contrast, for the irrigated grapevines, it is possible that higher water availability in the soil potentiated the leaching of mineral forms of N in the soil, especially N-NO3 -, which forms an outer sphere complex with the reactive particles in the soil. Thus, the N availability is reduced and, consequently, the amount absorbed and accumulated in grapevine organs decreases, including in the berries (BRUNETTO et al., 2006).

Figure 1  : Water potential in leaves (ψ) of grapevine cv. 'Pinot Nero', irrigated (I) and non-irrigated (NI). 

Table 1: Total content of nutrients in leaves and grapevine berries composition cv. 'Pinot Nero' irrigated (I) and non-irrigated (NI). 

(1)Means followed by the same letter in the column do not differ by Tukey test (P<5%). ns = not significant.I = irrigated, NI = non irrigated.

Irrigation also did not alter the weight of 100 berries or the pH, TSS, or total titratable acidity values in the must (Table 1). One explanation for the consistent weight of 100 berries is that the rainfall was approximately 57mm throughout August, which may have provided sufficient water to maintain adequate availability in the soil and supply to the plants. Sufficient water supply for plants allows for adequate rates of photosynthesis and sugar synthesis, promoting growth and increased cluster weight. However, despite these potential benefits of irrigation, there was no increase in the weight of 100 berries, which may explain the absence of an effect of irrigation on TSS content (COSTELLO & PATTERSON, 2012). The fact that plants may not have been stressed due to lack of water may explain the absence of effects of irrigation on pH and total titratable acidity (ETCHEBARNE et al., 2010). Although it produced a less negative water potential for the grapevine leaves, irrigation did not affect nutritional status or must composition, and it only slightly changed the berry nutrient content.

ACKNOWLEDGEMENTS

The authors thanks Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for its financial support (CAPES, Processo BEX 11571/13-6).

REFERENCES

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COSTELLO, M.J.; PATTERSON, K. Regulated deficit irrigation effect on yield and wine color of Cabernet Sauvignon in Central California. Hortscience, v.47, n.10, p.1520-1524, 2012. Available from: <http://hortsci.ashspublications.org/content/47/10/1520.short>. Accessed: Ago. 01, 2014. [ Links ]

ETCHEBARNE, F. et al. Leaf: fruit ratio and vine water status effects on Grenache Noir (Vitis vinfera L.) Beny composition: water, sugar, organic acids and cations. S Afr J Enol Vitic, v.31, p.106-115, 2010 [ Links ]

MARIANI, L. et al. Climate change in Europe and effects on thermal resources for crops. International Journal of Biometeorology, v.56, p.1123-1134, 2012. Available from: <http://link.springer.com/article/10.1007/s00484-012-0528-8>. Accessed: ago. 01, 2014 doi: 10.1007/s00484- 012-0528-8. [ Links ]

VAN LEEUWEN, C. et al. Vine water status is a key factor in grape ripening and vintage quality for red Bordeaux wine. How can it be assessed for vineyard management purposes. J Int Sci Vigne Vin, v.43, p.121-134, 2009. Available from: <http://www.oliviertregoat.com/vine_water_status_is_a_key_factor.pdf>. Accessed: Ago. 15, 2014. [ Links ]

Received: August 19, 2014; Accepted: October 24, 2014

Corresponding author: Marlise Nara Ciotta, email: mciotta@gmail.com

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