INTRODUCTION:
Fat is an important component of a diet, but in Western-type diets, the intake of saturated fatty acids (SFA) is higher in proportion to unsaturated fatty acids (UFA), and the total proportion of fatty acid (FA) intake is unbalanced. The saturated FA content of the total dietary energy intake should not be higher than 10%, and the content of monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA) should be up to 15% and 10% of the total daily energy intake. To achieve good body health conditions, the optimum ratio of FA n-6/n-3 should be low (RUSSO, 2009). The main health effect of UFA is attributed to n-3 FA (SIMOPOULOS, 1991), α- linolenic acid and its metabolites eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), which are essential for the body.
An increased content of UFA, particularly α- linolenic acid, in the diet has gained attention (WILLIARD, 2001). In case of humans, an increased UFA content can be achieved by increasing their contents in the consumed diet. The total content of n-3 PUFA increased in people who consumed meat from chickens fed with diets that were supplemented with linseed oil (HAUGH et al., 2012). Additionally, a linseed-supplemented diet had a positive effect on the FA composition from different adipose tissues (intramuscular and subcutaneous) in pigs. The FA composition of adipose and muscle tissues depends on diet, fat level, age and sex of the animals. Dietary FAs are incorporated into the adipose tissues of pigs without significant changes (BEE et al., 2008), and thus, the quality of the meat from monogastric animals can be easily affected by dietary manipulations.
Probiotics and prebiotics have favorable effects on the intestinal microflora. The intestinal microflora affects the lipid metabolism and modulates fat mass development in the body (WALL et al., 2009; DELZENNE et al., 2011), and therefore, it can be a dietary alternative that affects the content of FAs in a host tissue. Inulin-type fructans are considered prebiotics (ROBERFROID, 2007). Inulin, alone or in combination with horse chestnut, supports the growth of beneficial bacteria and reduces harmful bacteria in the gut and influences lipid metabolism in the host (LETEXIER et al., 2003; DEWULF et al., 2011; SZABADOSOVA et al, 2013).
In spite of the fact that prebiotics affect lipid metabolism, there is a little information about how these changes are reflected in the FA profile of muscles, and this effect was only studied in mice (DEWULF et al., 2011) or in chickens (VELASCO et al., 2010). Therefore, the objective of this study was to investigate the effect of linseed oil, inulin and horse chestnut added to a high fat (HF) diet fed to pigs on the content of FAs in the musculus Longissimus dorsi.
MATERIALS AND METHODS:
A total of 18 gilts (Landrace x Slovak White; initial BW 25 ± 0.5kg) were housed in balance cages. After a 5 d adaptation period, during which the pigs fed with a pig grower diet, there was a 70 d experimental period, during which the pigs received a basal HF diet supplemented with the tested substances. At the end of the experiment, the pigs were slaughtered, and the samples from the musculus Longissimus dorsi (MLD) were taken from the right half-carcass at the level of the last rib. The samples were frozen at -20ºC and stored for following analyses.
During the experiment, a basal HF diet with or without supplements was used (Table 1). A palm oil, containing a high content of SFA (FIORENTINI et al., 2015), which is responsible for negative alteration in the body (LAUGERETTE et al., 2015), was added to the diet in the amount of 13% to increase the content of fat in the diet. There were 3 experimental treatments: the control (CG, n = 6) was fed with the basal HF diet; the LG (n = 6) received a supplement of linseed oil ( Lini oleum virginale, n-6/n-3 in a ratio of 1/3, Dr. Kulich Pharma, Czech Republic) daily in the amount of 5ml per pig and the IG (n=6) received inulin (Beneo, Belgium) and horse chestnut ( Aesculus Hippocastanum ) (extract Hippocastani extractum siccum , Calendula, Slovak Republic) daily in the amounts of 4 and 1g per pig, respectively. Inulin was extracted with hot water from a chicory root and was enriched with oligofructose. This product was composed of a mixture of long-chain inulin and short-chain oligofructose (it contained 95% fructan chains and 5 % monosaccharides and disaccharides, such as fructose, glucose and sucrose). The pigs were fed twice daily at 07:00 and 16:00h in two equal doses at a daily rate of 85-90g kg-0.75. Water was available ad libitum. To ensure the accuracy of the addition of the tested substances, they were weighed for each animal and each feeding separately and mixed with feed allowance by hand. Nutrient content in the basal HF diet was analyzed in accordance with AOAC (1990) procedures. Homogenized samples of the MLD were analyzed for FAs after extraction from the MLD samples. Lipid extraction was performed with petrol ether on an SER 148/6 (Velp Scientifica, Italy). Fatty acid composition of muscle was determined by gas chromatography (GC 6890N, Agilent Technologies, USA). The FA content was analyzed as a methylester with flame-ionization detection on a capillary column DB-225 (length 30m, ID 0.53mm, thickness of stationary phase 1mikrometer) and was calculated as % share (as relative content) from the total sum of the FAs (C6-C24).
Table 1 Composition and analyzed content of nutrients in basal HF diet (as fed basis).

1Provided the following per kilogram of diet: vitamin A, 9000IU; vitamin D3, 1500IU; α- tocopherol, 35mg; vitamin B1, 1.7mg, vitamin B2, 6mg; vitamin B6, 2.5mg; Ca-pantothenate, 15mg; niacin, 38mg; vitamin K3, 2mg; biotin, 0.12mg; cyanocobalamin, 0.03mg; choline, 156mg; Fe, 103mg; Zn, 116.5mg; Mn, 49.0mg; Cu, 40mg; I, 1.2mg; Co, 0.4mg; Se, 0.3mg; lysine, 3.82g; methionine, 0.8g; and threonine, 1.72g.
The experimental data were subjected to an ANOVA using Statgraphic Plus 3.1 software. When the treatment effects were significant (P<0.05) or tended to be different (P<0.10), the means were separated using a Fisher's LSD procedure. Each pig was considered as an experimental unit. An analysis of covariance was conducted to evaluate the effect of the added substances on the concentration of the FAs in the MLD.
RESULTS:
The effect of the addition of linseed oil, inulin and horse chestnut to a HF diet on the composition of FAs in the MLD is shown in table 2. The addition of linseed oil increased (P=0.001) the content of α- linolenic acid in the LG treatment group by 58 and 61% when compared to CG and IG treatment groups, respectively. Similarly, the content of EPA and DHA was greater (P=0.031) in pigs that were fed with diet with linseed oil supplements when compared to the CG and IG treatments, in which EPA and DHA were not detected. The n-6/n-3 ratio in the pigs fed with the diet supplemented with linseed oil was 8.84, which was lower (P=0.001) than the CG and IG treatments, which were 14.07 and 14.74, respectively. The decrease was 31.2 %.
Table 2 Effect of diet on the FA composition in musculus Longissimus dorsi (%).

1CG - control group fed HF basal diet, LG - fed basal HF diet supplemented with linseed oil in daily amount 5ml pig-1, IG - fed basal HF diet supplemented with inulin in daily amount 4g pig-1 + horse chestnut in daily amount 1g pig-1.
abmeans in raw are significantly different.
The n-3/SFA ratio increased (P=0.007) by 57 % in the LG treatment group (0.04) when compared to the CG and IG treatment groups (0.02). Changes in the ratio of n-6/n-3 and n-3/SFA were caused by the increased content of α- linolenic acid, EPA and DHA. It was found that the addition of inulin and horse chestnut to the diet had no effect on the content of FAs in the MLD.
DISCUSSION:
The intake of a HF diet, especially one containing a high portion of SFA (palm oil in our study), is characterized by its negative effect on the body (FIORENTINI et al., 2015; LAUGERETTE et al., 2015). Dietary manipulations at the beginning of the food chain may increase the content of n-3 FA and mitigate the negative effect in humans with a HF diet. Pork is considered to be a main source of SFA, but the content of FAs can be changed by suitable dietary manipulations to pigs nutrition (ČERVEK et al., 2011). Linseed is a rich source of n-3 FA, particularly of α- linolenic acid (NITRAYOVÁ et al., 2014) from which n-3 FA with longer chain, EPA and DHA, are metabolized. The supplementation of linseed oil into the diet of pigs increased the content of α- linolenic acid in meat (ČERVEK et al., 2011). In the present study, the positive effect of the addition of linseed oil into a HF diet on the content of α- linolenic acid, EPA and DHA in the MLD was confirmed. Similarly, in other researches, the addition of linseed oil into the diet of pigs increased the concentration of n-3 FA in the body (KASTEĽ et al., 2007). Linseed oil supplemented into a HF diet decreased a ratio of n-6/n-3 in the MLD when compared with pigs fed a diet without supplemented linseed oil. Similar results were also reported in other studies (ČERVEK et al., 2011; HAUGH et al., 2012). Despite this, the ratio of n-6/n-3 was higher than the results reported by OKROUHLÁ et al. (2013) who also studied pigs fed with a diet with addition of linseed. The difference between studies was caused by the fact that the content of n-3 in our study was only increased slightly. Addition of linseed oil into the diets resulted in an increase in the n-3/SFA ratio. Similar results were also reported by NÜRNBERG et al. (2011). The n-6/n-3 ratio decreased and the n-3/SFA ratio increased as a result of the increased n-3 content in the MLD.
The microbial population influences the metabolism of cells in adipose tissues (DELZENNE et al., 2011). The metabolic activity of the enteric microbiota and the dietary manipulation thereof represent a realistic target for the modification of the FA composition in host tissues. WALL et al. (2009) reported that the oral administration of bifidobacteria increased the concentrations of EPA and DHA in the adipose tissue in mice. Because prebiotics have a positive effect on the intestinal microflora of the body (ROBERFROID, 2007), they can alter the metabolism of fats in the host tissue. For example, the prebiotic mannanoligosaccharide had a positive effect on lipid metabolism in terms of reducing serum cholesterol and abdominal fat in chickens (KANNAN et al., 2005). Additionally, inulin lowered blood lipids in human studies (BALCAZAR-MUNOZ et al., 2003; LETEXIER et al., 2003).
Although, information about the effect of prebiotics on the FA content in tissues is limited. DEWULF et al. (2011) reported that inulin-type fructans supplemented to a HF diet (31.6 % lard; 3.2 % soybean oil) did not modify the FA pattern in mice. In chickens, the addition of inulin in a diet containing sunflower oil increased the content of PUFA due to increase in α- linolenic acid and the decreased SFA content in the fat and muscle tissues, but this effect was not observed when palm oil was used (VELASCO et al., 2010). Similarly, in the present study, when inulin with horse chestnut was added into the HF diet, there was no impact on the composition of the FAs in the MLD. It can be speculated that it was probably a result of the low dose of the tested prebiotics and a subsequent low efficiency to suppress the negative effect of the HF palm oil diet in changes of composition of the gut microbiota (DELZENNE et al., 2011). Alternatively, the null result could be due to the fact that the effect of the prebiotics on the changes to the FA profile in the host tissue depends on the saturation of the dietary fat (VELASCO et al., 2010). In the study by VELASCO et al. (2010), the content of SFA in the palm oil diet was 3.4 times greater than in the sunflower diet, and in the study of DEWULF et al. (2011), the lard in the diet increased the content of SFA as well (MATTSON & LUTTON, 1958).
As mentioned above, the common diet contains a high content of saturated and a low content of unsaturated FAs. The required content and ratio of FAs in the human body can be achieved by changing the FA content in consumed products (HAUGH et al., 2012). The addition of linseed oil into the diet of pigs is a suitable way to increase the content of PUFA in the food chain of a consumer (ČERVEK et al., 2011), as well as, to suppress the negative effect of a HF diet in terms of the n-3 FA intake and the reduction of the n-6/n-3 ratio, which cannot be said about the addition of inulin and horse chestnut to the HF palm oil diet.
CONCLUSION:
The supplementation of linseed oil into a HF diet increased the content of α- linolenic acid, EPA and DHA in the MLD, which resulted in a reduction of the n-6/n-3 ratio in the MLD of pigs and; therefore, is a suitable way to increase the content of PUFA in pork meat and reduce the effect of a HF diet. In the present study, the addition of inulin and horse chestnut had no effect on the FA profile in the MLD.