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Different sources of sulfur in diets of adult cats on the urinary parameters and acid-base balance

Efeitos da adição de diferentes fontes de enxofre na dieta de gatos adultos em parâmetros urinários e equilíbrio ácido-básico

ABSTRACT:

Urolithiasis is a common disorder in the veterinary clinic and is considered as one of the most frequently cause of morbidity. This disorder is closely associated with urinary pH and nutrition plays a key role in the control of this disease, because through dietary manipulation it is possible to modify the urinary pH. Sulfur is considered macroelement with a strong influence on the acid-base status and may be crucial to control urinary pH in cats. The purpose of this study was to evaluate the effects of addition of different sources of sulfur (S) in the diet of cats on the urinary parameters and acid-base balance. Forty-two healthy adult cats were divided into 3 groups, and each group of 14 cats received 7 diets in a complete randomized block design. Calcium sulfate (CaSO4), DL-methionine (DLM) and methionine hydroxy analog (MHA) were added to a control diet in two levels (1.28g S/kg and 2.56g S/kg) to formulate 6 other experimental diets. The acid-base balance was evaluated by hemogasometry in samples of venous blood. The DLM at the highest level and MHA differed of the control diet in relation to urinary pH (P<0.05). Calcium sulfate; although, not differentiated from the control diet, has been shown to alter urinary pH despite its zero electrolyte balance. Apparently, the alkalizing effect of calcium was not sufficient to avoid sulfate acidification of the urine. Treatments showed no alteration of the acid-base balance of the animals and no affect the consumption of the diets.

Key words:
calcium sulfate; felines; methionine; methionine hydroxy analogue; urolithiasis

RESUMO:

A urolitíase é uma desordem comum na clínica veterinária, considerada como uma das maiores causas de morbidade. Esta desordem está intimamente associada ao pH urinário sendo que a nutrição desempenha papel fundamental no controle dessa doença, pois através da manipulação dietética é possível modificar o pH urinário. O enxofre é considerado um macroelemento com forte influência no equilíbrio ácido-básico e pode ser crucial para controlar o pH urinário em gatos. O objetivo deste estudo foi avaliar os efeitos da adição de diferentes fontes de enxofre (S) na dieta de gatos nos parâmetros urinários e no equilíbrio ácido-básico destes animais. 42 gatos adultos saudáveis foram divididos em 3 grupos e cada grupo de 14 gatos recebeu 7 dietas em um delineamento de blocos ao acaso. O sulfato de cálcio (CaSO4), a DL-metionina (DLM) e a metionina hidróxi-análoga (MHA) foram adicionados a uma dieta controle em dois níveis (1,28g S/kg e 2,56g S/kg) para formular outras 6 dietas experimentais. O equilíbrio ácido-básico foi avaliado por hemogasometria em amostras de sangue venoso. O DLM no teor mais alto e MHA diferiram da dieta controle em relação ao pH urinário (P<0,05). O sulfato de cálcio, embora não tenha diferido da dieta controle, demonstrou alterar o pH urinário apesar do seu equilíbrio eletrolítico nulo. Aparentemente, o efeito alcalinizante do cálcio não foi suficiente para anular a acidificação da urina pelo sulfato. Os tratamentos não apresentaram alteração do equilíbrio ácido-básico dos animais e não afetaram o consumo das dietas experimentais.

Palavras-chave:
felinos; metionina; metionina hidróxi-análoga; sulfato de cálcio; urolitíase

INTRODUCTION:

The macroelement composition of diets has a strong influence on the acid-base status and urinary pH of animals (KIENZLE et al., 1991KIENZLE, E. et al. Influence of food composition on the urine pH in cats. Journal of Nutrition, v.121, p.87-88, 1991. Available from: <Available from: http://jn.nutrition.org/content/121/11_Suppl/S87.long >. Accessed: Apr. 28, 2017.
http://jn.nutrition.org/content/121/11_S...
; ALLEN & KRUGER, 2000ALLEN, T.A., KRUGER, J.M. Small Animal Clinical Nutrition. In: Feline lower urinary tract disease. Topeka: Mark Morris Associates, 2000. p.689-723.; WAGNER et al., 2006WAGNER, E. et al. Influence of the feed base excess on urine parameters in cats. Journal of Animal Physiology and Animal Nutrition, v.90, p.19-24, 2006. Available from: <Available from: http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0396.2005.00613.x/abstract;jsessionid=0538070126D31BEB32E2A37D8AECED93.f03t02 >. Accessed: May 28, 2017. doi: 10.1111/j.1439-0396.2005.00613.
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). Treatment for urolithiasis in dogs and cats has been advent of new studies in the past century and as uroliths management is evolving, nutrition remains a subject of much clinical interest and debate in the management of these animals (LULICH et al., 2016LULICH, J.P. et al. ACVIM small animal consensus recommendations on the treatment and prevention of uroliths in dogs and cats. Journal of Veterinary Internal Medicine, v.30, p.1564-1574, 2016. Available from: <Available from: https://dx.doi.org/10.1111/jvim.14559 >. Accessed: May 11, 2017. doi: 10.1111/jvim.14559.
https://dx.doi.org/10.1111/jvim.14559...
) since the urine pH has strong influence on certain types of urolith formation (BARTGES & CALLENS, 2015BARTGES, J.W.; CALLENS, A.J. Urolithiasis. Veterinary Clinics: Small Animal Practice, v.45, p.747-768, 2015. Available from: <Available from: http://www.vetsmall.theclinics.com/article/S0195-5616(15)00046-7/fulltext >. Accessed: Jun. 07, 2017. doi: 10.1016/j.cvsm.2015.03.001.
http://www.vetsmall.theclinics.com/artic...
; BARTGES, 2016BARTGES, J.W. Feline Calcium Oxalate Urolithiasis: Risk factors and rational treatment approaches. Journal of Feline medicine and Surgery, v.18, p.712-22, 2016. Available from: <Available from: http://journals.sagepub.com/doi/abs/10.1177/1098612X16660442?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub%3Dpubmed& >. Accessed: Jun. 02, 2017. doi: 10.1177/1098612X16660442.
http://journals.sagepub.com/doi/abs/10.1...
).

Struvite uroliths are associated with alkaline pH (close to or greater than 7.0) (LANGSTON et al., 2008LANGSTON, C. et al. Diagnosis of urolithiasis. Compendium on Continuing Education for the Practising Veterinarian, v.30, p.447-455, 2008. Available from: <Available from: https://pdfs.semanticscholar.org/7f36/7a099b84146169dacdcd71e669630d773753.pdf >. Accessed: Apr. 28, 2017.
https://pdfs.semanticscholar.org/7f36/7a...
). Reduction of urinary pH has been shown to be effective in decreasing the incidence of formation of these crystals (MARKWELL et al., 1998MARKWELL, P.J. et al. The effect of diet on lower urinary tract diseases in cats. The Journal of Nutrition, v.128, p. 2753-2757, 1998. Available from: <Available from: https://www.ncbi.nlm.nih.gov/pubmed/9868257 >. Accessed: May 9, 2018.
https://www.ncbi.nlm.nih.gov/pubmed/9868...
), and supplements are recommended to maintain urine at a pH between 6.2 and 6.4 for the prevention of these uroliths and between pH 5.9 and 6.1 for dissolution (ALLEN & KRUGER, 2000ALLEN, T.A., KRUGER, J.M. Small Animal Clinical Nutrition. In: Feline lower urinary tract disease. Topeka: Mark Morris Associates, 2000. p.689-723.).

Addition of sulfur to cat diets may occur through sulfur-containing amino acids, like methionine, cysteine and taurine, and the supplementation of some microelements such as iron and copper sulfates. The DL-methionine (DLM) can be used to correct the dietary amino acid balance and also as a urinary acidifier (FUNABA et al., 2000FUNABA, M, T. , et al. Effect of supplementation of dry cat food with DL-Methionine and ammonium chloride on struvite activity product and sediment in urine. Journal of Veterinary medical Science, v.36, p.337-339, 2000. Available from: <Available from: https://www.jstage.jst.go.jp/article/jvms/63/3/63_3_337/_article >. Accessed: Apr. 28, 2017. doi: 10.1292/jvms.63.337.
https://www.jstage.jst.go.jp/article/jvm...
). Methionine hydroxy analog (MHA) has been recently introduced into the formulation of diets for farm animals in order to replace DLM. No studies with cats were reported with this molecule. Theoretically, it would have higher acidifying capacity compared to methionine. Due to the lack of nitrogen, MHA receives a nitrogen atom in the liver and transforms it into L-methionine, a process that could reduce the urinary excretion of nitrogen (ammonia), thus lowering the urine buffering capacity (MARTÍN-VENEGAS et al., 2006MARTÍN-VENEGAS, R. et al. Conversion of the methionine hydroxy analogue DL-2- hydroxy-(4-methylthio) butanoic acid to sulphur-containing amino acids in the chicken small intestine. Poultry Science, v.85, p.1932-1938, 2006. Available from: <Available from: https://www.ncbi.nlm.nih.gov/pubmed/17032826 >. Accessed: Apr. 28, 2017. doi: 10.1093/ps/85.11.1932.
https://www.ncbi.nlm.nih.gov/pubmed/1703...
). In other species, as in pigs, the pH of the urine could be acidified by the inclusion of calcium sulfate (CaSO4) in different doses, being a higher dose responsible for greater acidification (CANH et al. 1998CANH, T.T. et al. Influence of electrolyte balance and acidifying calcium salts in the diet of growing-finishing pigs on urinary pH, slurry pH and ammonia volatilization from slurry. Livestock Production Science, v.56, p.1-13, 1998. Available from: <Available from: http://www.sciencedirect.com/science/article/pii/S0301622698001481 >. Accessed: Jun. 07, 2017. doi: 10.1016/S0301-6226(98)00148-1.
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).

During food formulation, several approaches and ingredients can be used to balance macroelements in order to achieve a properly balanced diet. However, nutritionists need to have adequate information about the buffering potential of each mineral source to make the correct decision during food formulation. Therefore, the present study evaluated the effects of the addition of three sulfur sources [calcium sulfate (CaSO4), DLM and MHA] in the diet on different urinary parameters and acid-base equilibrium of adult cats.

MATERIALS AND METHODS:

Animals and experimental design

The experiment was conducted at the Laboratory for Research on Nutrition and Nutritional Diseases of Dogs and Cats - Universidade Estadual Paulista, Jaboticabal, Brazil. All experimental procedures were approved by the Animal Ethics and Welfare Commission of FCAV/UNESP, protocol number 017648/11. Cats belonged to the same laboratory colony. Cats’ health was assessed prior to the beginning of the survey by clinical and hematologic examination, as well as by urine analysis.

Forty-two healthy, adult, mixed-breed cats aged 4 ± 1.3 years and 4.1 ± 0.84kg of body weight and seven experimental dry diets for cats were used. The experiment was conducted with 3 groups and each group of 14 cats received 7 diets in a randomized complete block design (2 cats/diet in each group), resulting in 6 cats/diet. Each block had a duration of 13 days with an interval of 2 days. Three sulfur sources, calcium sulfate, DLM, and MHA were added at two levels, 1.28 and 2.56g S/kg of diet, into a basal extruded dry cat food with base excess (BE) around 100mEq/kg, previously ground in a cutting mill equipped with a 1mm screen. In the control diet, no sulfur sources were added. After mixing all the ingredients, diets were pelleted. Pelletization is a low shear and temperature processing, so changes on added ingredients is not expected. Nutritional level was similar in all diets (Table 1) and all were within recommendations for adult cat maintenance (AAFCO, 2008ASSOCIATION OF AMERICAN FEED CONTROL OFFICIALS (AAFCO). Methods for substantiating nutritional adequacy of dog and cat foods. Oxford: Association of American Feed Control Officials, 2008. Available from: <Available from: http://www.aafco.org >. Accessed: May 20, 2017.
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).

The base excess (mEq/kg) was calculated by the formula: EB=49.9×Ca+82.3×Mg+43.5×Na+25.6×K-64.6×P-62.4×S-(28.2×C1)…, the concentration of the elements being in g/kg DM.

Table 1
Ingredient and chemical composition of the experimental diets.

Cats were kept in individual stainless steel metabolic cages (90 x 80 x 90cm) equipped with a system to separate feces and urine. Cats were fed once daily (08:00h) and the metabolizable energy of diets was calculated from their chemical composition, through the equation estimation methodology described by the Nutrition Requirements of Dogs and Cats (NRC, 2006NATIONAL RESEARCH COUNCIL (NRC). Nutrient requirements of dogs and cats. Washington : The National Academies Press, 2006. 424p.). The amount of food supplied was calculated as 130 kcal ME per kg0.4 (NRC, 2006NATIONAL RESEARCH COUNCIL (NRC). Nutrient requirements of dogs and cats. Washington : The National Academies Press, 2006. 424p.). Food was offered placed ad libitum until the next meal. Food intake was daily measured by weighting offered food and refusals. Water was provided ad libitum.

Urine analysis

The first 7 days were used for diet adaptation and the last 6 days for total urine collection, in two periods. In the first period (days 8-10), urine was collected twice daily in plastic containers with 0.1g of thymol (0.1g per 100mL of urine) and kept refrigerated (4°C). Immediately after collection, urine volume was measured, as well as urine density with a refractometer (ATAGO CO., LTD; model T2-N3), and pH with a pH meter (model DM20, DIGIMED São Paulo, Brazil). The urine collected in 24h was maintained frozen. The urine produced in 72h was mixed and evaluated for sodium (mmol/L) and potassium (mmol/L) using an ion-selective method (AVL unit - OMNI4 - Roche, Brandford, USA), and chloride (mmol/L) was determined by the Labtest - mercuric thiocyanate methodology (Ref. 49 Chlorides - Labtest) followed by spectrophotometry. In the second period (days 11-13), urine was collected twice a day in plastic containers with HCl 6N (2mL per 100mL of urine) and kept refrigerated (4°C). This measure has been reported as sufficient to reduce the urinary pH to about 1-2, preventing the formation of crystals (GRIYTH & DUNN, 1978GRIYTH, D.P.; DUNN, D. Collection and preservation of urine for biochemical analyses. Investigative Urology, v. 15, p. 459-461, 1978. Available from: <Available from: https://www.ncbi.nlm.nih.gov/pubmed/649294 >. Accessed: Apr. 27, 2017.
https://www.ncbi.nlm.nih.gov/pubmed/6492...
). The urine collected in 24h was frozen. Calcium (mg/dL), phosphorus (mg/dL), magnesium (mg/dL) and sulfur (g/L) were measured in urine produced in 72h. Calcium quantification was analyzed using colorimetric method (CPC - cresolphthalein, calcium Liquiform Ref 90, Labtest, Minas Gerais, Brazil). Quantitative analysis of urinary phosphorus was made by a modified endpoint reaction UV method of Daly and Ertingshausen (Ref. 42 Liquiform UV Phosphorus, Labtest, Minas Gerais, Brazil), and magnesium by the colorimetric method (Magon sulfonate, magnesium Ref. 90, Labtest, Minas Gerais, Brazil). Sulfur was measure using the turbidimetric method as described by AOAC (2006)ASSOCIATION OF OFFICIAL ANALYTICAL CHEMISTS (AOAC). Official Methods of Analysis. Gaithersburgs: Association of Official Analytical Chemists, 2006, p. 1141..

Blood gas analysis

Blood gas analysis of venous blood was evaluated after 13d of diet consumption. At 08:00am (before feeding) and 02:00pm (six hours after feeding), 0.5mL of venous blood was collected from the medial saphenous vein without compression. Before blood was collected, the syringe was washed out with a heparin solution, leaving the dead-space filled. Blood was immediately analyzed for pH, sodium (Na), potassium (K), ionized calcium (Cai), the partial pressure of carbon dioxide (PCO2), bicarbonate (HCO3), base excess (BE) and osmolality (Osm) using Omini C Blood Gas Analyzer (Roche Diagnostics, Indianapolis, USA).

Chemical analysis

Food samples were ground in a cutting mill equipped with 1mm screen and analyzed for dry matter (DM) by submitting samples to oven drying; ash by muffle furnace incineration; and crude protein (CP) using the Kjeldahl method (AOAC, 2006ASSOCIATION OF OFFICIAL ANALYTICAL CHEMISTS (AOAC). Official Methods of Analysis. Gaithersburgs: Association of Official Analytical Chemists, 2006, p. 1141.). Samples were also analyzed for acid-hydrolyzed fat (AHF) using Soxhlet apparatus, and crude fiber (CF) using the Weende method (AOAC, 2006ASSOCIATION OF OFFICIAL ANALYTICAL CHEMISTS (AOAC). Official Methods of Analysis. Gaithersburgs: Association of Official Analytical Chemists, 2006, p. 1141.). Nitrogen-free extract (NFE) was calculated by subtracting ash, AHF, CP and CF components from DM (NFE = DM-ash-AHF-CP-CF). Minerals were analyzed after nitric-perchloric digestion. Phosphorus was measured with the spectrophotometer using the vanadate-molybdate method. Calcium, potassium, magnesium, chloride, and sodium were measured by flame atomic absorption spectrophotometry.

Statistical analysis

Data were analyzed using the statistical software SAS (version 9.2, SAS Institute Inc., Cary, USA); analyzed in a randomised block design by ANOVA, seven treatments composed of diets in three blocks, and the averages of treatments were compared by the SNK test. The blocking factor was time, design adopted as the laboratory cannot evaluate 42 cats simultaneously. For the urinary pH response, multiple regression analysis of the S levels was performed, comparing the different sources using the “Comparison of Regression Lines” module of Statgraphics plus 4.1. Values of P<0.05 were considered significant.

RESULTS:

Cats showed no clinical changes throughout the experiment. The average daily food intake was 12.3 ± 3.6g/kg of DM BW/d (46.9 ± 13.7kcal/kg of BW/d). Only the ionic calcium (Cai) was below normal hemogasometric parameters for adult cats in all experimental diets, before and after the food supply. Food consumption and blood gas values did not differ between groups (Table 2). The daily urine volume and urine density showed no significant differences among treatments. Urine pH was affected by treatments, being significantly different between control diet experimental diets, with DLM 2.56g S/kg and MHA at both doses of sulfur (P<0.001, Table 3).When the effect of sulfur source was verified, calcium sulfate showed lower efficiency in urine acidification when compared to the other sources (P<0.001). There were significant differences in the excretion of electrolytes. Cats fed with MHA diet (1.28 S) had increased renal excretion of Ca2+, Na+, K+, and Cl- when compared to the control diet. Phosphorus excretion was higher in cats fed with DLM diet (2.56 S) and MHA. Only cats fed with MHA diet (2.56 S) showed significantly higher excretion of sulfur compared to control diet (P<0.05; Table 3).

Table 2
Dry matter intake and ion and gas analysis of venous blood of cats.
Table 3
Urine characteristics and macroelements intake and excretion by urine of cats.

There was no correlation between urinary pH and electrolyte excretion. Sulfur excretion was correlated only with Mg2+ excretion (R2=0.55, P<0.001), and this demonstrated the high correlation with Na+ (R2=0.74, P<0.001), K+ (R2=0.74, P<0001), phosphorus (R2=0.72, P<0.001) and Cl- excretion (R2=0.70, P<0.001) (Table 4).

Table 4
Correlation between the electrolytes excreted in the urine of cats.

DISCUSSION:

The hypocalcemia reported in this study (reference values of Cai: 1.15 to 1.3, DIBARTOLA, 2006DIBARTOLA, S.P. Fluid therapy in small animal practice. Philadelphia: Pennsylvania, 2006. 611p.) may possibly be explained by the use of heparin in the syringe. Heparin tends to dilute ionized calcium, decreasing its value in the sample (SCHENCK & CHEW, 2008SCHENCK, P.A.; CHEW, D.J. Hypercalcemia: A quick reference. Veterinary Clinics of North America: Small Animal Practice, v.38, p.449-455, 2008. Available from: <Available from: http://www.sciencedirect.com/science/article/pii/S0195561608000338?via%3Dihub >. Accessed: Apr. 28, 2017. doi: 10.1016/j.cvsm.2008.01.020.
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) (Table 2).

The results showed that calcium sulfate showed lower efficiency in urine acidification compared to other sources (P<0.001). Electrolyte balance of calcium sulfate is zero because the positive divalent calcium charge counteracts the negative divalent anion sulfate (SO42-) charge. For this reason, calcium sulfate should not influence the acid-base balance and would not change urinary pH. The addition of calcium sulfate resulted in slight, but not significant, urinary acidification. This result may be related to the alkalizing effect of calcium, which was not strong enough to withdraw the acidifying effects of sulfur. Conversely, the BE of the diet with calcium sulfate was around 42mEq/kq, which is less than control diet (99.93mEq/kq) and causes a decrease in urinary pH, showing that other factors not only electrolyte balance can affect urinary pH.

CANH et al. (1998CANH, T.T. et al. Influence of electrolyte balance and acidifying calcium salts in the diet of growing-finishing pigs on urinary pH, slurry pH and ammonia volatilization from slurry. Livestock Production Science, v.56, p.1-13, 1998. Available from: <Available from: http://www.sciencedirect.com/science/article/pii/S0301622698001481 >. Accessed: Jun. 07, 2017. doi: 10.1016/S0301-6226(98)00148-1.
http://www.sciencedirect.com/science/art...
) investigated the effects of calcium sulfate (CaSO4), calcium chloride (CaCl2), calcium carbonate (CaCO3) and calcium benzoate (C14H10CaO4) in diets for finishing pigs. The authors added these sources based on the amount of calcium (7 and 10g Ca/kg) in the diet with high EB (320mEq/kg DM) and low EB (100mEq/kg DM). The authors observed that calcium sulfate at both doses acidified urine, and the highest dose led to the greatest acidification. The result was similar to urine acidification caused by the ingestion of diets containing calcium chloride. The authors explained that Cl- and SO42- anions have higher absorption rate in the intestine than Ca2+. Furthermore, Ca2+ is maintained in the body, especially in bones (KEMME-KROONSBERG, 1993KEMME-KROONSBERG, C. Nutrition and acid-base balance of pigs, a review. Lelystad : Institute for Animal Science and Health, 1993. 69p.), and has low urinary excretion and blood circulation (GUYTON & HALL, 2011GUYTON, A.C.; HALL, J.E. Tratado de fisiologia médica. Rio de Janeiro: Guanabara Koogan, 2011. 1176p.). Conversely, in the present study, the fact that calcium sulfate was the sulfur source less efficient in acidifying urine suggests that Ca2+ positively influenced urine pH, because all sources initially contained the same amount of sulfur. Another fact that may have influenced this parameter may be the different digestibility of the sources used, altering the effect of these and unbalancing the equal amounts of S in the diets, unfortunately in this study this was not evaluated.

DLM and MHA did not result in differences in urinary pH each other, but they differed in the control group. The acidifying effects of DLM supplementation were also confirmed by FUNABA et al. (2000FUNABA, M, T. , et al. Effect of supplementation of dry cat food with DL-Methionine and ammonium chloride on struvite activity product and sediment in urine. Journal of Veterinary medical Science, v.36, p.337-339, 2000. Available from: <Available from: https://www.jstage.jst.go.jp/article/jvms/63/3/63_3_337/_article >. Accessed: Apr. 28, 2017. doi: 10.1292/jvms.63.337.
https://www.jstage.jst.go.jp/article/jvm...
), who showed that the addition of 3% DLM acidified feline urine pH compared to the group without addition of DLM. When added at 1%, DLM did not show efficacy. In the present study, supplementation of 1.2% DLM was not effective in reducing urinary pH, but 2.56% was, in comparison to the control group (Table 3).

Studies about MHA as urinary acidifier have not received much attention and there are no published data regarding its effectiveness in cats. MIDDELBOS et al. (2006MIDDELBOS, I.S. et al. Effects of dietary supplementation of DL-methionine or 2-hydroxy-4-(methylthio)-butanoic acid on food intake, nutrient digestibility, nitrogen balance, urinary and blood metabolites in healthy, growing dogs. Archives of Animal Nutrition, v.60, p.301-316, 2006. Available from: <Available from: http://www.tandfonline.com/doi/full/10.1080/17450390600785483 >. Accessed: Apr. 28, 2017. doi: 10.1080/17450390600785483.
http://www.tandfonline.com/doi/full/10.1...
) evaluated the effect of DLM and MHA supplementation (0.1% and 0.2%) on canine urinary pH, and supplements were not effective in acidifying urine pH, perhaps because the doses used were very low. However, in the study of MIDDELBOS et al. (2006)MIDDELBOS, I.S. et al. Effects of dietary supplementation of DL-methionine or 2-hydroxy-4-(methylthio)-butanoic acid on food intake, nutrient digestibility, nitrogen balance, urinary and blood metabolites in healthy, growing dogs. Archives of Animal Nutrition, v.60, p.301-316, 2006. Available from: <Available from: http://www.tandfonline.com/doi/full/10.1080/17450390600785483 >. Accessed: Apr. 28, 2017. doi: 10.1080/17450390600785483.
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cats fed with a diet with 0.1% MHA had a lower concentration of postprandial urinary calcium (mmol/L) than cats fed with diets with 0.1% DLM (P<0.10), unlike results reported in our study, which MHA diets increased urine calcium excretion compared with calcium sulfate.

According to MARTÍN-VENEGAS et al. (2006MARTÍN-VENEGAS, R. et al. Conversion of the methionine hydroxy analogue DL-2- hydroxy-(4-methylthio) butanoic acid to sulphur-containing amino acids in the chicken small intestine. Poultry Science, v.85, p.1932-1938, 2006. Available from: <Available from: https://www.ncbi.nlm.nih.gov/pubmed/17032826 >. Accessed: Apr. 28, 2017. doi: 10.1093/ps/85.11.1932.
https://www.ncbi.nlm.nih.gov/pubmed/1703...
), MHA is converted into L-Met through two pathways: alpha carbon oxidation and transamination. The authors showed that there is a conversion of MHA into L-Met in the small intestines of chickens and MHA provides higher cysteine concentrations in relation to DLM, suggesting that MHA is preferably shifted through the transsulfuration route and subsequent formation of sulfate ions. A further explanation of the acidifying effect of MHA is that MHA does not have nitrogen in its molecule, and must receive an N in the liver to turn into L-methionine (L-Met). The result is lower nitrogen excretion, with lower ammonia excretion and lower acid buffering in the urine.

LEMANN & RELMAN (1959LEMANN, J.; RELMAN, A.S. The relationship of sulphur metabolism to acid-base balance and electrolyte excretion: The effects of DL-Methionine in normal man. Journal of Clinical Investigation, v.38, p.2215-2223, 1959. Available from: <Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC293313/ >. Accessed: May 19, 2017.
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) evaluated the effects of the DLM sulfur metabolism in humans. They observed that the urine pH seemed to be related to the acid-base balance, but was independent of the amount of sulfate excreted. Associated with urinary acidification, the authors observed an increase in “net acid”, mainly in the form of ammonium. They also observed high potassium, calcium and phosphate excretion, probably originated from titratable acids (buffers). Approximately 70% of sulfur supplied by DLM was excreted as inorganic sulfate. Reactions that lead to the formation of this compound are the sole source of endogenous acid derivatives from sulfur metabolism. The authors suggested that for each mole of oxidized sulfur, there is the production of two equivalents of hydrogen ions, being the hydrogen source part of the methionine metabolism, generated by oxidation of cysteine into cysteine sulphinic acid and deamination or transamination of cysteine sulphinate into ß-sulphinyl pyruvate. The final oxidation of sulfite to sulfate does not yield additional acid.

In relation to the urinary excretion of electrolytes in cats, few studies have evaluated such information and all are different from those verified in this study. In an earlier study, PALMORE et al. (1978PALMORE, W.P. et al. Effects of diet on feline urine. Laboratory Animal Science, v.28, p.551-555, 1978. Available from: <Available from: https://www.ncbi.nlm.nih.gov/pubmed/723217 >. Accessed: May 09, 2018.
https://www.ncbi.nlm.nih.gov/pubmed/7232...
) evaluated the dietary effects of feline urine. More recently, WAGNER et al. (2006WAGNER, E. et al. Influence of the feed base excess on urine parameters in cats. Journal of Animal Physiology and Animal Nutrition, v.90, p.19-24, 2006. Available from: <Available from: http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0396.2005.00613.x/abstract;jsessionid=0538070126D31BEB32E2A37D8AECED93.f03t02 >. Accessed: May 28, 2017. doi: 10.1111/j.1439-0396.2005.00613.
http://onlinelibrary.wiley.com/doi/10.11...
) verified the influence of feeding of excess bases on urine parameters in cats; and PASSLACK & ZENTEK (2013PASSLACK, N.; ZENTEK, J. Urinary calcium and oxalate excretion in healthy adult cats are not affected by increasing dietary levels of bone meal in a canned diet. PLoS One, v.5, p.e70530, 2013. Available from: <Available from: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0070530 >. Accessed: May 09, 2018. doi: 10.1371/journal.pone.0070530.
http://journals.plos.org/plosone/article...
) investigated the impact of calcium (Ca) and phosphorus (P) on the feline urine composition. Table 3 shows the results of the urinary excretion of electrolytesreported in this study. Calcium excretion was influenced by sulfur source. Urinary calcium excretion was quite low (less than 0.5% of calcium intake) and an increase in urinary calcium was observed in MHA treatments, even though it biological importance is possible negligible. LULICH et al. (2004LULICH, J.P. et al. Effects of diet on urine composition of cats with calcium oxalate urolithiasis. Journal of the American Animal Hospital Association, v.40, p.185-191, 2004. Available from: <Available from: http://jaaha.org/doi/10.5326/0400185?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dpubmed >. Accessed: May 09, 2017. doi: 10.5326/0400185.
http://jaaha.org/doi/10.5326/0400185?url...
) reported urinary calcium excretion of 0.018 ± 0.015mmol/kg of body weight (BW) per 24h in cats fed with a diet containing 0.84% calcium, which is similar to values reported in this study. In relation to Mg2+, the mean value found by LULICH et al. (2004) LULICH, J.P. et al. Effects of diet on urine composition of cats with calcium oxalate urolithiasis. Journal of the American Animal Hospital Association, v.40, p.185-191, 2004. Available from: <Available from: http://jaaha.org/doi/10.5326/0400185?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dpubmed >. Accessed: May 09, 2017. doi: 10.5326/0400185.
http://jaaha.org/doi/10.5326/0400185?url...
was 0.033 ± 0.016mmol/kg BW per 24h in cats fed with a diet containing 0.07% Mg2+, less than values reported in this study because diets contained practically twice as much; however, urinary pH influences the Mg excretion, and the diets of the studies and the sources used are different, which makes the comparison between studies difficult. They reported urinary Na+ concentration of 1.149 ± 0.524 and K+ of 1.684 ± 0.479mmol/kg BW per 24h in cats fed with diet containing 0.28% Na+ and 0.72% K+, values similar to those found in this experiment. K+ excretion is mostly urinary (90-95%) and in situations of acidosis, increased extracellular potassium occurs because the high availability of H+ ions decreases the activity of the Na+/K+ pump, reducing the uptake of K+ by cells, which increases the extracellular concentration and increases urinary excretion. Similar results regarding increased K+ excretion were found by LEMANN & RELMAN (1959LEMANN, J.; RELMAN, A.S. The relationship of sulphur metabolism to acid-base balance and electrolyte excretion: The effects of DL-Methionine in normal man. Journal of Clinical Investigation, v.38, p.2215-2223, 1959. Available from: <Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC293313/ >. Accessed: May 19, 2017.
https://www.ncbi.nlm.nih.gov/pmc/article...
). No differences were reported in Na+ excretion when assessing the relationship between sulfur metabolism and excretion of electrolytes in humans.

LULICH et al. (2004LULICH, J.P. et al. Effects of diet on urine composition of cats with calcium oxalate urolithiasis. Journal of the American Animal Hospital Association, v.40, p.185-191, 2004. Available from: <Available from: http://jaaha.org/doi/10.5326/0400185?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dpubmed >. Accessed: May 09, 2017. doi: 10.5326/0400185.
http://jaaha.org/doi/10.5326/0400185?url...
) found 0.530 ± 0.271mmol/kg BW of phosphate per 24h in cats fed with a diet containing 0.68% P. The amounts of phosphate excretion were close to those reported in this study. However, phosphate excretion was significantly lower in cats fed with a diet containing calcium sulfate (2.56 S) compared to a diet containing MHA (1.28 S). This may be due to the higher Ca2+ intake, which reduces the urinary excretion of phosphorus and magnesium. This can be explained by the formation of an insoluble calcium-magnesium-phosphate complex in the lumen of the intestine, which lowers the concentration of soluble phosphorus and magnesium (PASTOOR et al., 1994PASTOOR, F.J. et al. Increasing calcium intakes lower urinary concentration of phosphorus and magnesium in adult ovariectomized cats. Journal of Nutrition, v.124, p.299-304, 1994. Available from: <Available from: http://jn.nutrition.org/content/124/2/299.long >. Accessed: Apr. 28, 2017.
http://jn.nutrition.org/content/124/2/29...
).

LULICH et al. (2004LULICH, J.P. et al. Effects of diet on urine composition of cats with calcium oxalate urolithiasis. Journal of the American Animal Hospital Association, v.40, p.185-191, 2004. Available from: <Available from: http://jaaha.org/doi/10.5326/0400185?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dpubmed >. Accessed: May 09, 2017. doi: 10.5326/0400185.
http://jaaha.org/doi/10.5326/0400185?url...
) found mean chloride concentration of 1.716 ± 0.842mmol/kg BW per 24h in cats fed with a diet containing 0.6% chlorine. The Cl- values reported in this experiment were higher. One possible explanation may be acidosis due to increased concentration of H+ ions, which combine with ammonia generating ammonium ion, being excreted as ammonium chloride (ALLEN & KRUGER, 2000ALLEN, T.A., KRUGER, J.M. Small Animal Clinical Nutrition. In: Feline lower urinary tract disease. Topeka: Mark Morris Associates, 2000. p.689-723.).

CONCLUSION:

The DLM at the 2.56g S/kg level and the MHA at the levels, 1.28 and 2.56 g S/kg acidified the urinary pH. Calcium sulfate; although, not differentiated from the control diet, has been shown to alter pH despite its zero electrolyte balance. Apparently, the alkalizing effect of calcium was not sufficient to void sulfate acidification of the urine. Treatments showed no alteration of the acid-base balance of the animals or affect the consumption of the diets.

ACKNOWLEDGEMENTS

To Mogiana Alimentos Ltda (Guabi), for the financial, technical support. To Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) foundation for the author supported.

REFERENCES:

  • CR-2018-0017.R2

BIOETHICS AND BIOSSECURITY COMMITTEE APPROVAL

  • BIOETHICS AND BIOSSECURITY COMMITTEE APPROVAL

    All experimental procedures were approved by the Ethics Research Committee for Animal Welfare of the College of Agrarian and Veterinary Sciences, Universidade Estadual Paulista (protocol number 017648/11).

Publication Dates

  • Publication in this collection
    2018

History

  • Received
    15 Jan 2018
  • Accepted
    08 Aug 2018
  • Reviewed
    06 Sept 2018
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