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Ciência Rural

Print version ISSN 0103-8478On-line version ISSN 1678-4596

Cienc. Rural vol.50 no.2 Santa Maria  2020  Epub Feb 03, 2020

http://dx.doi.org/10.1590/0103-8478cr20190077 

ANIMAL PRODUCTION

Corn supplementation on milk urea nitrogen content of dairy cows grazing on temperate annual pasture

Efeito da suplementação com grão ou silagem de milho sobre o nitrogênio ureico no leite de vacas em pastos anuais de inverno

Aline Cristina Dall-Orsoletta1 
http://orcid.org/0000-0003-1915-7174

João Gabriel Rossini Almeida1 

Márcia Maria Oziemblowski1 
http://orcid.org/0000-0002-2016-1157

Henrique Mendonça Nunes Ribeiro-Filho1  * 
http://orcid.org/0000-0002-4455-6866

1Programa de Pós-graduação em Ciência Animal, Universidade do Estado de Santa Catarina (UDESC), 88520-000, Lages, SC, Brasil..


ABSTRACT:

The excretion of urinary nitrogen (N), one of the most important environmental contaminants from livestock systems, is highly correlated with milk urea N content. The objective of this research was to evaluate the use of different types of corn supplementation on milk urea N in grazing dairy cows. Twelve Holstein × Jersey lactating dairy cows were divided into six uniform groups according to milk production, lactation stage and live weight. Treatments were compared according to a 3 × 3 replicated Latin square experimental design, with three periods of seventeen days (twelve days to adaptation and five to measurements). The experimental treatments were exclusively grazing (G); grazing + supplementation with 4.2 kg DM of corn silage (CS) and grazing + supplementation with 3.2 kg DM of ground corn (GC). The pasture used was annual ryegrass (Lolium multiflorum L.) and white oats (Avena sativa L.). The milk protein production increased 65 g/day in the GC treatment group compared to the G and CS groups. The supplemented dairy cows showed lower milk urea N (-2.8 mg/dL) than unsupplemented cows, but the N utilization efficiency (g N output in milk/ g N intake) did not change between treatments (average = 0.26). Additionally, there was a relationship between milk and plasma urea nitrogen concentrations (R2 = 0.64). In conclusion, for dairy cows grazing annual temperate pastures, corn ground supplementation increased milk protein production and reduced the excretion of milk urea N, whereas corn silage reduced the excretion of milk urea N without affecting milk protein production.

Key words: nitrogen excretion; ground corn; corn silage; grazing

RESUMO:

A excreção de nitrogênio (N) urinário é um dos mais importantes contaminantes ambientais nos sistemas de produção animal, e possui alta correlação com as concentrações de N ureico no leite. Objetivou-se avaliar a suplementação com grão ou silagem de milho na excreção de N ureico e produção de proteína no leite de vacas em pastos anuais de inverno. Doze vacas em lactação cruza Holandês × Jersey foram divididas em seis lotes uniformes de acordo com a produção de leite, estádio de lactação e peso vivo. Os tratamentos foram comparados de acordo com um delineamento experimental em duplo Quadrado Latino 3 × 3, com três períodos de dezessete dias (doze dias de adaptação e cinco de avaliações). Os tratamentos experimentais foram exclusivamente pastejo (G), suplementação com 4.2 kg de MS de silagem de milho (CS) e 3.2 kg de MS de milho moído (GC). O pasto utilizado foi Azevém anual (Lolium multiflorum L.) e Aveia branca (Avena sativa L.). A produção de proteína no leite aumentou 65 g/dia nos animais do grupo GC quando comparados aos dos grupos G e CS. As vacas suplementadas reduziram os teores de N ureico do leite em comparação às não suplementadas (-2,8 mg/dL), mas a eficiência de uso do N (g N produzido no leite/g N ingerido) foi semelhante entre os tratamentos (média = 0,26). Além disso, houve uma relação positiva entre as concentrações de N ureico no leite e no plasma (R2 = 0.64). Em conclusão, a suplementação com o grão de milho se mostrou eficiente na redução da excreção de N ureico com aumento na produção de proteína do leite, enquanto a silagem de milho permitiu reduções na excreção de N ureico sem afetar a produção de proteína do leite.

Palavras-chave: excreção nitrogênio; milho moído; silagem de milho; pastejo

Nitrogen (N) deposition on terrestrial ecosystems has increased significantly during the last decades, causing soil acidification, water eutrophication, decreases in biodiversity, and increases in greenhouse gas emissions. The most important gases involved in these processes are ammonia (NH3) and nitrous oxide (N2O). A large portion of these gaseous emissions come from animal N excretion. In mammals, the main form of N excretion occurs as milk, feces, and urine urea. Following its synthesis, the urea equilibrates rapidly throughout body fluids, including milk and urine. Consequently, studies have shown that is possible to evaluate the urinary N excretion and N utilization efficiency using the plasma urea N and, in practical situations, using the milk urea N (NOUSIAINEN et al., 2004).

The N utilization efficiency can be affected by the relationship between N and energy in the diet. In the case of ruminants that are fed fresh forages, ruminal N losses are often high due to the unbalanced level of high degradable N and the low fermentable energy in the forage. This leads to an inefficient utilization of forage N to microbial growth, increasing N-NH3 absorption and urinary N excretion (PEYRAUD & DELAGARDE, 2013), which might be mitigated by using supplements containing fermentable carbohydrates, such as ground corn and corn silage. Additionally, it has been shown that energy supply has a role in regulating post-absorptive N utilization and efficiency by increasing milk protein synthesis (RIUS et al., 2010).

In south Brazil, annual ryegrass and oat are the major fresh forage species used in grazing dairy systems. These species contain high CP and, consequently, energy supplementation may be an alternative to improve the efficiency of forage protein utilization. However, the relationship between feeding strategies commonly used in dairy systems in the south of Brazil and N excretion needs to be better studied. The aim of this study was to evaluate the effect of corn silage and ground corn supplementation for dairy cows grazing annual temperate pastures on milk urea N excretion and to quantify the relationship between milk urea N and plasma urea N in this condition.

All data presented in this research were measured concomitantly in an experiment in which the objective was to assess the CH4 emissions (DALL-ORSOLETTA et al., 2019). Thus, a more detailed description of experimental procedures can be reported there. Briefly, the experiment was performed from August to November 2016 in Lages, SC, Brazil (50.18ºW, 27.47ºS; 920 m altitude) in accordance with the regulations of the Santa Catarina State University Ethical Committee (Protocol no. 43.73.09.08.16). Twelve Holstein × Jersey multiparous cows were separated into 6 homogeneous groups according to milk production (23.3 ± 6.9 kg/d), days in milk (101 ± 57.6 days), and body weight (492 ± 76.8 kg). Each group was randomly assigned to treatment sequences according to a replicated 3 × 3 Latin square design. The experimental period lasted 17 days and consisted of 12 days of adaptation and 5 days of measurement. Treatments consisted of cows with access to annual ryegrass + oat without supplementation (G) or with different energy supplementation - ground corn (GC) and corn silage supplementation (CS). The amount of ground corn was limited to 4 kg/day to avoid drastic modifications on ruminal environment, and the corn silage was calculated to offer the same net energy lactation (NEL) provided by the ground corn supplementation. The NEL of supplements was estimated using the equations published by INRA (2007). The quantities offered were 4.2 and 3.6 kg (as DM basis) of corn silage and ground corn, respectively.

The ryegrass (Lolium multiflorum cv. Barjumbo) and oat (Avena sativa cv. FUNDACEP - FAPAR 43) pastures were sown in May 2016 on a total area of 6.5 ha. The area was split into six paddocks, two per treatment, and was grazed by the same two cows during each period. The grazing method was strip grazing, and the herbage allowance (HA) was adjusted to obtain the same post-grazing sward height among treatments. The area allocated daily to each treatment group was calculated by estimating the pre-grazing herbage mass (LANTINGA; et al. 2004). The HA above ground level in the G, CS, GC groups were 35, 30.8 and 34.6 kg DM/cow/day, respectively.

The cows were milked twice daily (7 a.m. and 3:30 p.m.). Over the last 5 days of each period, individual milk production was recorded, and individual milk samples were collected to determine the milk urea N concentrations. The herbage DM intake was estimated in each group by determining the difference between the pre- and post-grazing herbage mass values. The milk urea N was determined by infrared spectrophotometry (Dairy Spect FT, Bentley Instruments Inc, Chaska, Minnesota, USA). The plasma urea N was determined by individual blood samples. Samples were collected into vacutainer tubes without anticoagulant on the morning of the first (day 13) and last days (day 17) of each experimental period by jugular venipuncture. Tubes were centrifuged (2,000 × g for 10 min), and the plasma was then separated and stored at -20 ºC until it was analyzed for urea.

The ruminal degradable protein (RDP) balance was calculated from the difference between the supply of the truly digestible protein when the degradable nitrogen limits microbial growth (PDIN) and the supply of the truly digestible protein when the available energy limits microbial growth (PDIE) (INRA, 2007). For details about grazed herbage and supplement sampling and analyses see DALL-ORSOLETTA et al. (2019). The CP, NDF and ADF contents of grazed herbage averaged 212, 447 and 236 g/kg DM, respectively. The grazed herbage had a good nutritive value; on average, the NEL was 1.67 Mcal/kg DM and 107 g of truly protein digestible in the small intestine per kg of DM. For the corn silage and ground corn, the CP values were 61 and 85 g/kg DM, the NEL values were 1.53 and 1.82 Mcal/kg DM, and the truly digestible protein values in the small intestine were 65 and 87 g/kg DM, respectively.

The dependent variables were subjected to an analysis of variance using the function PROC MIXED in the software SAS (version 9.3, SAS Institute, Cary, NC, USA) with a model that included the random effect of square, the random effect of cow nested in square, the random effect of period, and the fixed effect of treatment.

The milk production and milk protein production increased in the GC cows compared to the G and CS groups (Table 1). The supplemented dairy cows showed lower milk and plasma urea N than unsupplemented dairy cows, but the N utilization efficiency (g N output in milk/g N intake) did not change between treatments. In a comprehensive review, CASTILLO et al. (2000) showed that N efficiency utilization in dairy cows decreased exponentially when N intake is greater than 400 g/day. In the current work, the N intake of G and CS treatments was approximately 400 g/day, increasing to 482 g/day in GC cows. Thus, it is logical to assume that cows receiving ground corn supplementation did not reduce N efficiency utilization because they improved their milk protein production, which was probably a consequence of greater fermentable energy intake. In contrast, cows receiving corn silage had lower metabolizable protein supply but maintained their milk protein production by reducing the N urinary excretion, as indicated by reductions in milk and plasma urea concentrations when compared to G cows.

Table 1 Dry matter intake, milk production, protein balance, milk and plasma urea N concentrations of dairy cows grazing annual ryegrass (Lolium multiflorum cv. Barjumbo) and oat (Avena sativa cv. FUNDACEP - FAPAR 43) without supplementation or supplemented with corn silage or ground corn. 

----------------------Treatments¹---------------------
Item G CS GC SEM P-value
DM intake, kg/d
Herbage 11.9a 10.1b 11.2ab 0.18 <0.001
Corn silage - 3.1 - - -
Ground corn - - 3.6 - -
Total 11.9c 13.2b 14.8a 0.19 <0.001
Milk production, kg/day 21.7b 21.8b 23.1a 0.38 0.030
Milk fat, % 4.34 4.36 4.25 0.053 0.312
Milk protein, % 3.13b 3.07c 3.21a 0.020 <0.001
Milk fat, g/day 931b 938ab 974a 12.7 0.056
Milk protein, g/day 673b 662b 733a 11.2 <0.001
Crude protein intake, g/day 2609 b 2498 b 3018 a 80.0 0.006
PDIN supply, g/day2 1682b 1387c 1800a 37.21 <0.001
PDIE supply, g/day3 1278b 1170c 1518a 25.35 <0.001
RDP balance, g/day4 404a 217c 282b 14.56 <0.001
N efficiency utilization, g/g5 0.266 0.269 0.251 0.006 0.140
Milk urea N, mg/dL 18.1a 15.2b 15.4b 0.37 <0.001
Plasma urea N, mg/dL 15.4a 11.9b 11.2b 0.51 <0.001

1Treatments: G; cows grazing annual ryegrass + oat without supplementation, GC; 4 kg ground corn supplementation, CS; 15 kg corn silage supplementation. Means followed by the same letter are not significantly different (P>0.05).

2Truly digestible protein when degradable nitrogen limits microbial growth.

3Truly digestible protein when available energy limits microbial growth.

4Ruminal degradable protein balance.

5g N output in milk/g N intake.

The plasma urea N concentration recorded in this research was linearly related to the milk urea N concentration (Figure 1), and both were reduced when the RDP balance was improved (Figure 1b). The relationship between plasma and milk urea N reflects the rapid equilibration of blood urea into milk, and a positive linear coefficient is expected when values of milk urea N were not greater than 25 mg/dL (BURGOS et al., 2007). In the current study, milk urea N did not exceed 22 mg/dL. Additionally, both corn silage and ground corn supplementation improved the RDP balance, reducing plasma and milk urea N. These results may be, at least partially, explained because temperate herbage grasses are known to have protein contents usually greater than 140 g/kg DM, a value that is considered the limit for ruminal nitrogen loss (PEYRAUD & DELAGARDE, 2013), increasing plasma and milk urea N concentrations. In this study, the CP content of the herbage was 210 g/kg DM on average, and the RDP balance decreased from 404 to 258 g/day in the G group when compared to the CS and GC groups.

Figure 1 a) Relationship between ruminal degradable protein (RDP) balance vs plasma urea N and milk urea N in grazing dairy cows unsupplemented or supplemented with energy supplementation. b) Relationship between milk urea N vs plasma urea N (Milk urea N, mg/dL = 0.54 plasma urea N, mg/dL + 9.3; R2 = 0.64). 

In conclusion, a moderate level of corn ground supplementation for dairy cows grazing an herbage with good nutritive value was an effective feeding strategy for decreasing the milk urea N excretion by increasing milk protein production, whereas corn silage supplementation decreased milk urea N excretion without affecting milk protein production.

ACKNOWLEDGMENTS

This work was partially supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico - Brasil (CNPq) - Financie Codes 403754/2016-0 and 306313/2016-2, Fundação de Amparo à Pesquisa e Inovação do Estado de Santa Catarina (FAPESC) - Finance Code TR 584 2019, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001 and Fundo de Apoio à Manutenção e ao Desenvolvimento da Educação Superior - FUMDES, SC - Brasil (Public call nº 008/SED/2014).

REFERENCES

BURGOS, S. A. et al. Prediction of ammonia emission from dairy cattle manure based on milk urea nitrogen: Relation of milk urea nitrogen to ammonia emissions. Journal of Dairy Science, 2007. v.90, p.5499-5508. Available from: <Available from: https://doi.org/10.3168/jds.2009-2415 >. Accessed: Jan. 25, 2019. doi: 10.3168/jds.2009-2415. [ Links ]

CASTILLO, A. et al. A review of efficiency of nitrogen utilisation in lactating dairy cows and its relationship with environmental pollution. Journal of Animal and Feed Sciences, 2000. v.9, n.1, p.1-32. Available from: <Available from: https://doi.org/10.22358/jafs/68025/2000 >. Accessed: Nov. 28, 2018. doi: 10.22358/jafs/68025/2000. [ Links ]

DALL-ORSOLETTA, A. C. et al. Enteric methane emission from grazing dairy cows receiving corn silage or ground corn supplementation. Animal Feed Science and Technology, 2019. v.253, n. May, p.65-73. Available from: <Available from: https://doi.org/10.1016/j.anifeedsci.2019.05.009 > Accessed: Oct. 28, 2019. doi: 10.1016/j.anifeedsci.2019.05.009. [ Links ]

INRA. Alimentation des bovins, ovins et caprins. Besoins des animaux - valeurs des aliments. Tables Inra 2007. 4. ed. [S.l.]: [s.n.], 2007. [ Links ]

NOUSIAINEN, J. et al. Evaluation of milk urea nitrogen as a diagnostic of protein feeding. Journal of Dairy Science, 2004. v.87, p.386-398. Available from: <Available from: https://doi.org/10.3168/jds.S0022-0302(04)73178-1 >. Accessed: Apr. 11, 2018. doi: 10.3168/jds.S0022-0302(04)73178-1. [ Links ]

PEYRAUD, J. L.; DELAGARDE, R. Managing variations in dairy cow nutrient supply under grazing. Animal, 2013. v.7, n.1, p.57-67. Available from: <Available from: https://doi.org/10.1017/S1751731111002394 >. Accessed: Apr. 11, 2018. doi: 10.1017/S1751731111002394. [ Links ]

RIUS, A. G. et al. Interactions of energy and predicted metabolizable protein in determining nitrogen efficiency in the lactating dairy cow. Journal of Dairy Science, 2010. Available from: <Available from: https://doi.org/10.3168/jds.2008-1777 >. Accessed: Jan. 25, 2019. doi: 10.3168/jds.2008-1777. [ Links ]

CR-2019-0077.R1

Received: January 31, 2019; Accepted: November 11, 2019; Revised: December 16, 2019

E-mail: henrique.ribeiro@udesc.br. *Corresponding author

DECLARATION OF CONFLICT OF INTEREST

The authors declare no conflict of interest. The founding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results.

AUTHORS’ CONTRIBUTIONS

The authors contributed equally to the manuscript.

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