Print version ISSN 0103-9016
Sci. agric. (Piracicaba, Braz.) vol.63 no.2 Piracicaba Mar./Apr. 2006
Medidas de reflectância hiperespectral a campo para estimar produtividade de grãos e altura de plantas de trigo
Alexandre Cândido XavierI; Bernardo Friedrich Theodor RudorffII, *; Mauricio Alves MoreiraII; Brummer Seda AlvarengaII; José Guilherme de FreitasIII; Marcus Vinicius SalomonIII
IUniversidade Federal do Espírito Santo - UFES, C.P. 16 - 29500-000 - Alegre, ES - Brasil
IIInstituto Nacional de Pesquisas Espaciais - INPE, C.P. 515 - 12201-970 - São José dos Campos, SP - Brasil
IIIInstituto Agronômico Campinas - IAC, C.P. 28 - 13020-902 - Campinas, SP - Brasil
Hyperspectral crop reflectance data are useful for several remote sensing applications in agriculture, but there is still a need for studies to define optimal wavebands to estimate crop biophysical parameters. The objective of this work is to analyze the use of narrow and broad band vegetation indices (VI) derived from hyperspectral field reflectance measurements to estimate wheat (Triticum aestivum L.) grain yield and plant height. A field study was conducted during the winter growing season of 2003 in Campinas, São Paulo State, Brazil. Field canopy reflectance measurements were acquired at six wheat growth stages over 80 plots with four wheat cultivars (IAC-362, IAC-364, IAC-370, and IAC-373), five levels of nitrogen fertilizer (0, 30, 60, 90, and 120 kg of N ha-1) and four replicates. The following VI were analyzed: a) hyperspectral or narrow-band VI (1. optimum multiple narrow-band reflectance, OMNBR; 2. narrow-band normalized difference vegetation index, NB_NDVI; 3. first- and second-order derivative of reflectance; and 4. four derivative green vegetation index); and b) broad band VI (simple ratio, SR; normalized difference vegetation index, NDVI; and soil-adjusted vegetation index, SAVI). Hyperspectral indices provided an overall better estimate of biophysical variables when compared to broad band VI. The OMNBR with four bands presented the highest R2 values to estimate both grain yield (R2 = 0.74; Booting and Heading stages) and plant height (R2 = 0.68; Heading stage). Best results to estimate biophysical variables were observed for spectral measurements acquired between Tillering II and Heading stages.
Key words: remote sensing, agriculture, vegetation indices
Dados hiperespectrais de reflectância de culturas agrícolas são úteis para diversas aplicações e ainda existe a necessidade de estudos para definir as melhores bandas para estimar parâmetros biofísicos de culturas. O objetivo deste trabalho é analisar o uso de índices de vegetação (IV) de bandas estreitas e largas obtidas de medidas de reflectância hiperespectral a campo para estimar a produtividade de grãos e a altura de plantas do trigo (Triticum aestivum L.). Um experimento a campo foi conduzido durante a entre safra de 2003 em Campinas, São Paulo, Brasil. Medidas de reflectância foram adquiridas ao longo de seis estádios da cultura em 80 parcelas (quatro cultivares, cinco níveis de adubação nitrogenada e quatro repetições). Os IV testados foram: a) de banda estreita ou hiperespectrais (1. ótima reflectância de múltiplas bandas estreitas, OMNBR; 2. índice de vegetação da diferença normalizada com banda estreita, NB_NDVI; 3. primeira e segunda derivada da reflectância e; 4. quatro índices de derivadas da vegetação verde) e b) de banda larga (razão simples, SR; índice vegetativo da diferença normalizada, NDVI e; índice vegetativo ajustado para solo, SAVI). Os índices hiperespectrais forneceram melhores estimativas quando comparados às estimativas dos IV de banda larga. O índice OMNBR com quatro bandas apresentou os maiores valores de R2 para estimar a produtividade de grãos (R2 = 0,74; Emborrachamento e Espigamento) e a altura das plantas (R2 = 0,68; Espigamento). Os melhores resultados foram observados entre os estádios de Perfilhamento II e Espigamento.
Palavras-chave: sensoriamento remoto, agricultura, índices de vegetação
Broad band vegetation indices (VI) are ordinarily used to estimate biophysical parameters that can be incorporated in models to predict evapotranspiration and crop yield (Myneni et al., 1995). These indices are based on both high absorption of visible solar radiation by plant pigments and high scattering of near-infrared solar radiation by intercellular air spaces in the leaf mesophyll (Gates et al., 1965). The most widely used VI to estimate biomass, leaf area index (LAI) and absorbed photosynthetically active radiation (Asrar et al., 1984, Turner et al., 1999, Xavier & Vettorazzi, 2004) are Simple Ratio (SR; Jordan, 1969) and Normalized Difference Vegetation Index (NDVI; Rouse et al., 1974). More recently, Huete (1988) developed the Soil-Adjusted Vegetation Index (SAVI) to reduce soil background effects.
With the advancement of hyperspectral radiometers not only for laboratory or field but also for orbital measurements (e.g. Hyperion sensor on board of EO-1; Earth Observation-1, 2003), new methods to analyze spectral reflectance data were developed. For example, spectral derivative analysis of vegetation spectral reflectance measurements appear to be less sensitive to soil background reflectance effects (Demetriades-Shah et al., 1990). Elvidge & Chen (1995) and Chen et al. (1998) developed several indices from spectral derivative reflectance curves in the red-edge region (620-795 nm) due to their low sensitivity to soil background variations, when compared to broad band VI. On the other hand, Broge & Leblanc (2001) found that hyperspectral data are not better than broad band data to estimate LAI values higher than 2.8.
In the present work, wheat spectral reflectance field measurements were analyzed in terms of narrow and broad band vegetation indices to estimate final grain yield and plant height at several growth stages during crop growing season.
MATERIAL AND METHODS
Experimental Site and Treatments
A field study was carried out during the winter growing season of 2003 in Campinas, São Paulo State, Brazil (22º51'47" S; 47º04'42" W). The experiment was set up at a 4 x 5 factorial scheme, randomized, complete-block design (n = 4), and data were submitted to regression analysis to estimate biophysical variables; treatment effects were not analyzed. Treatments were applied to plots with four wheat (Triticum aestivum, L) cultivars (IAC-362, IAC-364, IAC-370 and IAC-373), and five levels of nitrogen fertilizer (0, 30, 60, 90 and 120 kg of N ha-1). Plots were 3 m long, 1.2 m wide and 0.8 m apart. Wheat was sown in June 2003 in rows spaced 0.15 m apart, with approximately 80 seeds per linear meter of row. Phosphorus and potassium were applied at a rate of 40 and 90 kg ha-1, respectively, while boron and zinc were applied at rates of 0.5 and 1.0 kg ha-1, respectively. All fertilizers were applied at seeding except for nitrogen, which was applied 50% at seeding and 50% was topdressed 30 days after plant emergence, according to the nitrogen fertilizer protocol. Irrigation was applied several times during the wheat growing season to supplement seasonal rainfall.
Final grain yield and plant height were measured at maturity. To eliminate 'edge-effects' a central area of 1.36 m2 was harvested in each plot. Wheat grains were oven-dried at 80ºC until 13% humidity was reached (Carneiro et al., 2005). Five plants per plot were used to estimate average final plant height.
Field reflectance measurements were performed over 80 wheat plots with the FieldSpec Pro FR spectroradiometer (Analytical Spectral Device Inc., 2003), at six different growth stages, from early vegetative growth until maturation (Table 1). The radiometric measurements were collected under clear-sky conditions between 10h00 and 11h00, at 1.2 m above crop canopy, with the 25º lens allowing 0.22 m2 field of view areas. One spectral reflectance measurement from the most central part of each plot was taken with the FieldSpec Pro FR over the spectral range 400 2,500 nm. These measurements were then used to simulate the narrow-bands and broad-bands from the spectral bands of the Hyperion (Earth Observation-1, 2003), and Thematic Mapper (Earth Observation Satellite Company, 1985) sensors, respectively. Due to atmospheric radiation absorption, some bands in the spectral ranges 1,350-1,440 nm; 1,790-1,990 nm; and 2,360-2,500 nm were disconsidered.
Vegetation Indices and Regression Models
The most widely broad band VI used in this work were:
Simple Ratio (SR; Jordan, 1969)
Normalized Difference Vegetation Index (NDVI; Rouse et al., 1974)
and Soil-Adjusted Vegetation Index (SAVI; Huete, 1988)
where: B3 (630-690 nm) and B4 (760-900 nm) are simulated spectral bands from the Enhanced Thematic Mapper Plus (ETM+) sensor on board of Landsat-7 remote sensing satellite, and L is a constant value equal to 0.5 applied to minimize soil background effects (Huete, 1988).
The regression models used were:
where B is the biophysical dependent variable (wheat grain or plant height); VI is the Vegetation Index independent variable (SR, NDVI or SAVI); a0 and a1 are the regression parameters; and m is the degree of the model. Polynomial and exponential models were both used to improve estimates of biophysical variables from spectral variables (Turner et al., 1999; Xavier & Vettorazzi, 2004).
Reflectance data were analyzed using several methods described in the literature: optimum multiple narrow-band reflectivity (OMNBR, Thenkabail et al., 2000; 2004); narrow-band NDVI (NB_NDVI; Thenkabail et al., 2000); best first- and second-order derivative of reflectance (Demetriades-Shah et al., 1990); and green vegetation indices based on derivatives (Chen et al., 1998; Elvidge & Chen, 1995). The OMNBR was defined using the MAXR procedure of Statistical Analysis System (SAS) version 6.12 (SAS Institute, 1997) to find the combination of narrow spectral bands that best predicted model biophysical variable (maximized the R2) at each wheat growth stage
where B is the biophysical variable; NB is the narrow-band reflectance of band j (j = 1,..., n); n is the number of Hyperion bands; and a is the regression parameter. For 1, 2, 3 and 4 narrow-bands we have, respectively:
The narrow-band NDVI (NB_NDVI) (Thenkabail et al., 2000) is defined as:
where i and j are band numbers from 1 to 242 allowing 242 x 242=58,564 combinations of NB_NDVI for each biophysical variable. Regression coefficients R2 between all possible narrow-bands and biophysical variables were determinate using a routine developed with MatLab (MatLab-MathWorks, 2002), that verified for each growth stage the two narrow-bands combination that provided highest R2 values.
The best first- and second-order derivatives of reflectance (1_Der and 2_Der) were used to reduce background reflectance influence (Demetriades-Shah et al., 1990) and were computed as:
where NB'(li) and NB''(li) are, respectively, the first- and second-order derivatives at the midpoint of band i (i = number of narrow-bands). Highest R2 values for first- and second-order derivatives were determinated using a linear regression model:
To generate the derivative green vegetation indices (1DL_DGVI, 1DZ_DGVI, 1DL_MDGVI and 2DZ_DGVI), the first- and second-order derivatives of reflectance were integrated to calculate the area between the wavelengths of 630 nm and 793 nm (Elvidge & Chen, 1995; Chen et al., 1998):
where NB'(li) - NB'(l1)>0;
where l1 and ln are the first (l = 630 nm) and last (l = 793 nm) narrow-bands to be integrated, respectively. The difference among the derivative green vegetation indices is the base line used as reference in the integration. For 1DL_DGVI, the reference used is the soil base line that considers the value of the first-order derivative at 630 nm, NB'(l1); for 1DZ_DGVI, the base line is zero; and for 1DL_MDGVI, the reference is also the soil base line, but the computed area is limited for values where the first-order derived is higher than the soil base line. Subtraction of the local derivative base line removes the portion of the red and near-infrared slop effect of the background, improving the vegetation index (Elvidge & Chen, 1995). The next step consisted to obtain the regression coefficients (R2) between these indices and the biophysical variables using linear regression models.
RESULTS AND DISCUSSION
Wheat grain yield and final plant height ranged from 1,628 kg ha-1 to 5,593 kg ha-1, and from 58 cm to 97 cm, respectively. The relatively large variation observed in these data is mainly attributed to different N treatments and to some extent to differences among genetic material. Moreira et al. (2005) studied spectral reflectance variation among 20 wheat genotypes, and registered that spectral reflectances and final biomass weight, for three of the genotypes used in the present work (IAC 364, IAC 370 and IAC 373), were quite similar. Also, significant improvements in R2 values were not observed for regressions performed by cultivar; therefore, regression analysis was performed by pooling genotypes.
Correlation coefficient between grain yield and plant height was 0.50. Figure 1 indicates that data asymmetry was not observed.
Figure 2 shows the average spectral reflectance behavior at each growth stage for the 80 plots. Spectral variations among curves are mainly due to differences in crop growth stages. Lowest reflectance values in near infrared wavelengths (NIR; l ~ 700 - 1300 nm) were observed for the early developmental stage (Tillering I), where biomass is low and reflectance is influenced mainly by soil. Maximum reflectance in the NIR was observed for the Jointing stage, which is coincident with highest values of green leaf area index, consequently low reflectance of solar radiation in red wavelengths and high scattering of solar radiation in NIR (Moreira et al., 1999). At Heading and Maturation stages, reflectance in visible (VIS; 350 - 700 nm) and NIR regions increase and decrease, respectively, when compared to the previous growth stages, which is mainly caused by the increase of senescent leaves.
Correlation coefficients (r) between each narrow-band and grain yield (Figure 3a) and plant height (Figure 3b) at different growth stages are presented in Figure 3. The pattern of r curves for grain yield and plant height, at distinctive growth stages, were similar; although, r in absolute values for plant height were smaller than those for grain yield. Lowest absolute r values were observed at Tillering I as a result of low crop development at this growth stage. Maximum negative values of r were observed at Heading stage for grain yield, and at Booting stage for plant height in the 680 nm wavelength, which corresponds to high solar radiation absorption by chlorophyll pigments. For the red-edge region (630-793 nm), a high increase of r values was observed, which is coincident with the reflectance increase of vegetation in this region (Figure 2). Greater positive r values were recorded between 760-880 nm wavelengths. For grain yield, the maximum positive r value observed was 0.72 at 902 nm during Booting stage. For plant height, the maximum positive r value observed was 0.62 at 813 nm during Jointing stage. Similar results were also observed by Yang & Chen (2004) for several biophysical parameters.
Table 2 presents the regression coefficients for the relationship between biophysical variables and OMNBR, NB_NDVI, and broad band VI (SR, NDVI and SAVI) at different growth stages. In general, greater R2 values were observed at full crop development during Booting and Heading stages, for all independent variables. On the other hand, lowest R2 values were observed at Tillering I and Maturation stages, when green biomass was low.
The OMNBR presented increased R2 values as the number of narrow-bands were added to the regression model. Best regression for grain yield (R2 = 0.74) and plant height (R2 = 0.68) were obtained with four narrow-bands at Heading stage. A significant increase in R2 values was observed for each new narrow-band incorporated in the model. For instance, at Booting stage 16% of wheat grain yield variation was explained with one narrow-band and up to 46% was explained with four narrow-bands. These results are in agreement with those obtained by Thenkabail et al. (2004) and Yang & Chen (2004). The most frequent four-narrow-bands in the OMNBR model were those from NIR (l ~ 700 nm - 1,300 nm), followed by short wave infrared (SWIR; l ~ 1,300 nm - 2,400 nm) and VIS (l ~ 350 nm - 700 nm) indicating the relevance of the combined use of spectral bands from these three regions (NIR, SWIR, and VIS) for vegetation studies (Thenkabail et al., 2000; 2004).
The NB_NDVI index explained up to 67% of grain yield variation and up to 65% of plant height variation for Booting stage (Table 2). Best results were observed for bands in the NIR region for grain yield and in the VIS region for plant height.
Table 2 also presents the broad band VI and its relationship to both grain yield and plant height as well as the model type. Best overall R2 values, for grain yield and final plant height, during Jointing and Heading stages, were achieved with SR vegetation index using the linear model. Best individual result for broad band VI was obtained for both SAVI (R2 = 0.60) and SR (R2 = 0.59) to estimate grain yield during Heading and Booting stages, respectively. These results are in agreement with those reported by Tucker (1979), Turner et al. (1999), and Xavier & Vettorazzi (2004), studying several other biophysical variables.
Figure 4 shows the scatter plots for the grain yield estimates provided by the broad band VI that yielded best R2 values in Table 2. Relationships were positive, as expected (Asrar et al., 1984). In theory, plants with higher production capacity should have higher LAI values and consequently higher VI values.
Figures 5a and 5b present the reflectance spectra, during Heading stage, for the lowest (1,629 kg ha-1) and highest (5,593 kg ha-1) wheat grain yield plots, respectively. The less developed plants from the lowest grain yielding plot reflected more VIS solar radiation and scattered (reflected and transmitted) less NIR radiation (Figure 5a). In contrast, the highest grain yielding plot (Figure 5b) presented low VIS reflectance and high NIR reflectance, and affected differently the first- and second-order derivatives for the low and high grain yielding plots, as observed in Figure 5(c-f). The area to be integrated for the low grain plot is much smaller than the one for the high grain plot for both first (Figure 5c, d; 1DZ_DGVI) and second-order derivative (Figure 5e, f; 2DZ_DGVI ).
Table 3 shows R2 values for estimates of grain yield and plant height from first-order derivative of reflectance, second-order derivative of reflectance and derivative of green vegetation indices at different wheat growth stages. Greatest R2 values were obtained with the first-order derivative, but similar results were also achieved with the second-order derivative, both with bands from the NIR region. Best growth stages to estimate biophysical variables from spectral reflectance measurements were within Jointing and Heading stages.
Regression coefficients were similar among derivative green vegetation indices (1DL_DGVI, 1DZ_DGVI, 1DL_MDGVI and 2DZ_DGVI) with lower overall performance when compared to: first-order derivative, second-order derivative (Table 3) or even with broad band VI (Table 2).
Figure 6 (a, b) shows that in most cases both grain yield and plant height were better estimated from hyperspectral indices (OMNBR, NB_NDVI, first- and second-order derivative indices) than from broad band indices. Major improvements of explained variance were observed early and late in the season. However, from Tillering II to Heading stages, when plants had a significant amount of green material, the broad band vegetation indices performed close to the narrow band VI, especially the NB_NDVI and the derivative indices. Among the hyperspectral indices, the OMNBR performed best in terms of R2 values (Figure 6).
Hyperspectral indices provided an overall better estimate of biophysical variables when compared to broad band VI. Major performance improvements with hyperspectral indices were observed early and late in the season when crop green leaf material was quite low. Among the several hyperspectral VI analyzed in this study the OMNBR with four bands presented highest R2 values to estimate both grain yield (R2 = 74% at Booting and Heading stages) and plant height (R2 = 68% at Heading stage). Best results to estimate biophysical variables, independently of narrow or broad band VI, were observed for spectral measurements acquired between Tillering II and Heading stages. Broad band VI performed almost as well as narrow band VI derived from hyperspectral bands for wheat crop with significant amount of green material.
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Received August 16, 2005
Accepted March 16, 2006