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Scientia Agricola

Print version ISSN 0103-9016

Sci. agric. (Piracicaba, Braz.) vol.72 no.2 Piracicaba Feb. 2015

http://dx.doi.org/10.1590/0103-9016-2014-0110 

FOOD SCIENCE AND TECHNOLOGY

Fatty acid profile, color and lipid oxidation of organic fermented sausage during chilling storage as influenced by acid whey and probiotic strains addition

Karolina Maria Wójciak*  1 

Małgorzata Karwowska1 

Zbigniew Józef Dolatowski1 

1University of Life Sciences in Lublin – Dept. of Meat Technology and Food Quality – 8 Skromna – 20-704 – Lublin – Poland.


ABSTRACT

Organic fermented sausages typically spoil during long-term storage due to oxidative rancidity. The application of natural antioxidants to meat stuffing is a major practice intended to inhibit the oxidation process and color changes. This study aimed to assess the effect of two unusual starter cultures: three probiotic strains (Lactobacillus casei LOCK 0900, Lactobacillus casei LOCK 0908 and Lactobacillus paracasei LOCK 0919) and lactic acid bacteria from acid whey on model fermented sausage type products focusing on oxidative stability by measuring instrumental color (L*, a*, b* values), conjugated dienes (CD), TBARS immediately after 21 days of ripening (0) and after 90 and 180 days of refrigerated storage (4 ºC). Determination of fatty acid composition, in meat product was performed after ripening and after 180 days of storage. At the end of the storage period, the salted sausages were characterized by the same content of polyunsaturated fatty acids (PUFA) compared to cured samples. The addition of acid whey and a mixture of probiotic strains to nitrite-free sausage formulation was barely able to protect lipids against oxidation in comparison to nitrite during vacuum storage. Surprisingly, the use of acid whey has an influence on the desired red-pinkish color of organic fermented sausage after ripening and after 180 days of storage period.

Key words: oxidation stability; uncured; functional food; antioxidant

Introduction

Organic meat product is produced according to standards that take into account both environmental and animal welfare. An important principle of organic production practice is that producers use a minimum of chemical substances during processing. According to EU regulations, preservative nitrite is allowed in organic meat production, but procedures in Poland have now been agreed on that will exclude the addition of nitrite to organic meat products in the very near future. This is reflected in EU Directive 2006/52/EC, referring to the necessity of reducing the use of nitrites (European Communities, 2006).

Nitrite is not only the multifunctional food additive responsible for the suitable color of cured meats but also serves as a strong antioxidant to protect flavor (Cassens, 1995). However, nitrite can cause the formation of carcinogenic N-nitrosoamines in cured products owing to its reaction with secondary amines and amino acids in muscle proteins (Zhang et al., 2007).

Fermented meat products typically spoil during long-term storage due to oxidative rancidity. Oxidative processes in fermented meat products lead to the degradation of unsaturated fatty acids, cholesterol and proteins (including pigments), which, in turn, contribute to the deterioration in color, texture (cross linking binding) and flavor (Zanardi et al., 2002). Moreover, the presence of sodium chloride can have an accelerating effect on lipid oxidation (Higgins et al., 1999).

The application of natural antioxidants to the meat stuffing is a major practice to inhibit the oxidation process and color changes (Pegg et al., 2000). The application of whey and whey proteins as antioxidants was investigated by several authors (Lasik et al., 2011; Liu et al., 2007; Tong et al., 2000; Worobiej et al., 2008). Whey protein was found in muscle food as a strong antioxidant which has the ability to chelate pro-oxidant transition metals by lactoferrin and serum albumin and free radical scavenging by cysteine and tyrosine. Moreover, whey proteins can also stimulate the growth of some Bifidobacterium and Lactobacillus probiotic strains (Kozioł et al., 2013).

Since there is a lack of knowledge about the effectiveness of acid whey and a mixture of probiotic strains to retard the oxidation processes in fermented meat product, this study aimed to assess the effect of a starter culture of probiotic strains as well as acid whey on model fermented sausage type products in terms of oxidative stability, by measuring instrumental color, conjugated dienes (CD), TBARS and fatty acid composition after 21 days of ripening (0) and during 180 days of refrigerated storage (4 ºC).

Materials and Methods

Preparation of acid whey

Fresh organic acid whey was obtained from cottage cheese production from the local traditional dairy processing plant. Fresh organic milk not preserved was then exposed to the process of natural fermentation. Next, it was heated to a temperature of 40 °C. When the coagulation process finished, the whey was separated from the lump. The count of lactic acid bacteria (LAB) in acid whey was 7.0 log CFU mL–1. A microbiological test to determine the LAB was performed according to ISO, 2002.

Characterisation and preparation of probiotic strains

The Lactobacillus caseiLOCK 0900, Lactobacillus casei LOCK 0908 and Lactobacillus paracasei LOCK 0919 strains (Motyl et al., 2009a,b,c) were isolated from the feces of healthy infants. In vitro tests conducted on probiotic strains revealed the strains’ resistance to low pH and bile salts (Cukrowska et al., 2009) and showed antagonistic activity against pathogenic bacteria. Moreover, the probiotic strains also synthesize bacteriocins resistant to proteolytic enzymes, e.g., biosurfactans with described antimicrobial function. A favorable impact of Lactobacillus casei LOCK 0900 strain on atopic dermatitis caused by cow’s milk protein allergy in children was also discovered (Cukrowska et al., 2010). Cukrowska et al. (2010) proved for in vivo experiments that a mixture of L. casei LOCK 0900, L. caseiLOCK 0908 and L. paracasei LOCK 0919 strains affects the immune system by inducing TH1 and regulatory cytokine production and by suppressing pro-allergic response better as compared to a single strain.

The probiotic material comprised a mixture of probiotic strains Lactobacillus caseiLOCK 0900, Lactobacillus caseiLOCK 0908 and Lactobacillus paracaseiLOCK 0919 according to the method described by Jaworska et al. (2011).

Sausage formulation

The materials were obtained from Polish Large White purebred swine in an organic system. The live weight of the animals was 120-130 kg. The animals were fed with feed from their own farms, consisting of organic cereal, namely wheat (Triticum aestivum), barley (Hordeum vulgare L.), oats (Avena sativa) and peas (Pisum sativum), grain and green matter. Meat and pork backfat were purchased from an organic meat manufacturer at 48 h postmortem. Pork meat (ham muscle 850 g kg–1) was first minced using a knife and chilled at 4 °C. Pork backfat (150 g kg–1) was also minced using a knife. The pork meat was divided into four equal samples.

The curing mixture (994-995 g kg–1 sodium chloride, 5-6 g kg–1 sodium nitrite) was added to one of the stuffings at a number of 28 g kg–1 of the meat (control – cured sample). Grecian sea salt without nitrite and nitrate (salt specification) was added to the remaining stuffing samples at a level of 28 g kg–1 of the sausage batter. The meat was then stored at 4 °C for 48 h. Next, each meat sample was minced separately using universal machine 8 mm discs and mixed with minced backfat.

Four batches were prepared: control with the curing mixture and 50 g kg–1 of water (C), organic sample with sea salt and 50 g kg–1 of water (S), organic sample with sea salt and acid whey added in a ratio of 50 g kg–1 (AW), organic sample with sea salt and 2 ml kg–1 starter cultures of probiotic strain added (P). Glucose was added to all treatments at a level of 6.0 g kg–1. The mixes were stuffed into casings (65mm) and matured according to the following procedure: 24 h at 19-20 °C and 90-92 % relative humidity, followed by a gradual decrease in temperature to 18-19 °C and relative humidity to 65-80 %, and then the sausage was maintained under these conditions for 19 days, giving a total of 21 days of maturation.

Following maturation, the sausages were divided into 12 samples (four treatments × three storage times), vacuum packed in low-density polyethylene (LDPE) bags and stored in a refrigerator (4 °C) for 180 days. Each sample was analyzed for conjugated dienes, TBARS values and L*, a*, b* color parameters immediately after maturing (day 0), and after 90 and 180 days of chilling storage. Fatty acid composition was measured at 0 and 180 days of refrigerated storage.

Composition of fatty acids

An analysis of fatty acid composition was carried out on total lipids extracted according to Folch et al. (1957) after methyl-esterification according to the procedure described in Method 969.33 (AOAC, 2005). A gas chromatographic analysis was performed as described by Official Method 996.06 (AOAC, 2005) using a chromatograph equipped with a capillary fused silica column. Flame ionization detection was used. The temperature of both the injection port and the detector was 260 ºC. The carrier gas was helium, 75.8 kPa and the sample volume 0.5 µL.

Fatty acid methyl esters were identified by comparison with previously run standards, alone or together with samples. The fatty acid methyl esters were quantified as a percentage of total methyl esters. Fatty acids were reported as a percentage of total fatty acids determined. Results were expressed as saturated fatty acids (SFA), monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA); and the PUFA/SFA (P/S), PUFA/SFA-stearic acid (C18:0) and MUFA+PUFA/SFA-stearic acid (C18:0), n-6/n-3 ratios were calculated.

Analysis of conjugated dienes

The formation of conjugated dienes was determined as described by Srinivasan et al. (1996). Meat samples (3.0 g) were suspended in 30 mL of distilled water and homogenized to form a “smooth slurry”. A 3 mL aliquot of this suspension was mixed with 30 mL of extracting solution (3:1 hexane : isopropanol) for 1 min. After centrifugation at 2,000 x g for 5 min, the absorbance of the supernatant was read at 233 nm. The concentration of the conjugated diene was calculated using the molar extinction coefficient of 25.000 M–1 cm–1 and the results were expressed as μmol mg–1 meat sample.

Determination of TBARS

Lipid oxidation was determined by assessing TBARS values according to the Pikul et al. (1989) method. The color intensity produced in the reaction of malondialdehyde with 2-thiobarbituric acid was measured using the Nicolet Evolution 300 spectrophotometer at a wavelength of 532 nm. TBARS values were expressed in mg malondialdehyde per 1 kg of meat product.

Instrumental color measurement

Instrumental color measurements were taken immediately after slicing the sample after 90 and 180 days of storage. This means that the samples were protected from progressive pigment degradation due to oxygen, light and higher temperature. After slicing, the samples were covered with a single layer of food wrap. Five readings were taken for each sample. The L*, a*, b* values and reflectance values between 390 and 700 nm were measured using an X-Rite 8200 colorimeter and the D65 illuminant, 8 mm port size, and a 10º standard observer. Color coordinates were determined using the CIELAB system. The results were expressed as lightness (L*), redness (a*) and yellowness (b*).

Statistical analysis

The data reported were analyzed by two-way analysis of variance (ANOVA) at p ≤ 0.05. The significance of the differences between treatments at the same storage time and the same treatments at different storage times was determined (p ≤ 0.05) using the T-Tukey’s range test. All data presented the averages from a minimum of two experiments conducted in duplicate.

Results and discussion

Composition of fatty acid

Lipid oxidation is a major cause of chemical fermented meat product spoilage and is considered one of the main limiting shelf-life factors of these products (Frankel 1998). Fatty acids of model fermented sausage shown in Table 1. After ripening, there was no difference (p > 0.05) in fatty acid content with one exception in MUFA content between control sample (C) and sample with acid whey addition (AW). There were no differences (p > 0.05) between percentage of polyunsaturated fatty acids (PUFA) and 18:2 (n-6), 18:3 (n-3); and between samples with curing salt compared to organic sausage samples.

Table 1 – Fatty acid composition (means ± standard deviations) of uncured model fermented sausage after 21 days of ripening (0) and 180 days of chilling storage (% of total fatty acids). 

Fatty acid Storage C S AW P
  day        
16:0 0 20.27 ± 0.39aB 21.05 ± 0.34aA 21.14 ± 1.28aB 20.37 ± 0.08aB
180 25.84 ± 0.53aA 22.19 ± 0.88bA 26.45 ± 0.30aA 25.81 ± 0.76aA
18:0 0 8.77 ± 0.16aB 9.40 ± 0.21aB 9.31 ± 0.74aB 9.18 ± 0.47aB
180 12.72 ± 0.83aA 13.10 ± 0.63aA 14.19 ± 0.93aA 13.57 ± 0.60aA
18:1 (n-9) 0 46.57 ± 0.21aA 45.56 ± 0.20aB 45.14 ± 0.69aA 45.14 ± 0.28aA
180 47.21 ± 0.59aA 52.46 ± 0.22bA 46.25 ± 0.40aA 45.74 ± 0.03aA
18:2 (n-6) 0 11.69 ± 0.10aB 11.66 ± 0.14aB 11.67 ± 1.27aB 12.03 ± 0.18aB
180 6.71 ± 0.55aA 7.40 ± 1.25aA 5.53 ± 0.27aA 6.42 ± 1.29aA
18:3 (n-3) 0 1.11 ± 0.04aA 1.02 ± 0.24 aA 1.21 ± 0.19aB 1.28 ± 0.04aB
180 0.32 ± 0.41 aA 0.64 ± 0.15 aA 0.41 ± 0.03aA 0.53 ± 0.17aA
SFA 0 31.22 ± 0.58aB 32.70 ± 0.43aA 32.76 ± 1.99aB 31.95 ± 0.41aB
180 40.70 ± 1.49aA 37.51 ± 1.61aA 43.00 ± 1.20aA 41.59 ± 1.65aA
MUFA 0 55.86 ± 0.44aB 54.53 ± 0.05abA 54.17 ± 0.57bB 54.62 ± 0.13abA
180 51.79 ± 0.96aA 54.05 ± 0.30aA 50.81 ± 1.49aA 51.06 ± 0.06aA
PUFA 0 12.82 ± 0.17aB 12.71 ± 0.35aB 12.98 ± 1.37aB 13.36 ± 0.22aB
180 7.42 ± 0.55aA 8.18 ± 1.31aA 6.15 ± 0.30aA 7.29 ± 1.59aA
n-3 0 1.11 ± 0.04aB 1.02 ± 0.24aA 1.21 ± 0.19aA 1.28 ± 0.04aB
180 0.71 ± 0.00A 0.79 ± 0.06A 0.62 ± 0.03A 0.71 ± 0.06A
n-6 0 11.69 ± 0.10aB 11.66 ± 0.14aB 11.73 ± 1.18aB 12.03 ± 0.18aB
180 6.71 ± 0.55aA 7.40 ± 1.25aA 5.53 ± 0.27aA 6.42 ± 1.29aA
P/S 0 0.41aA 0.39aA 0.40aA 0.42aA
180 0.18aB 0.22aB 0.14aB 0.18aB
P/S-C18:0 0 0.57aA 0.55aA 0.55aA 0.59aA
180 0.27aB 0.34aB 0.21aB 0.26aB
MUFA+PUFA/SFA-C18:0 0 3.06aA 2.89aA 2.86aA 2.99aA
180 2.12aB 2.55aA 1.98aB 2.08aB
n-6/n-3 0 10.53aA 11.43aA 9.69aA 16.90aA
180 9.45aB 9.37aB 8.92aA 9.04aB

Means followed by the same letters within columns (lower case letters) and row (capital letters) are not different (p < 0.05). SFA, saturated fatty acids; MUFA, monounsaturated fatty acids; PUFA, polyunsaturated fatty acids; P/S, polyunsaturated fatty acids/saturated fatty acids; P/S-C18:0, polyunsaturated fatty acids/saturated fatty acids – stearic acid; MUFA+PUFA/SFA-C18:0, monounsaturated fatty acids + polyunsaturated fatty acids/ saturated fatty acids - stearic acid. C - control with curing mixture and water, S - organic sample with sea salt and water, AW - organic sample with sea salt and acid whey added, P - organic sample with sea salt and starter cultures of probiotic strain added.

SFA are considered to raise plasma cholesterol, except for stearic acid which reduces total and LDL cholesterol and the MUFA have a hypocholesterolemic effect, but they do not decrease HDL cholesterol, which protects against cardiovascular diseases (Romero et al., 2013). Thus, the PUFA/SFA-C18:0 ratio and MUFA+PUFA/SFA-C18:0 ratio (Romero et al., 2013) were calculated for sausage and are shown in Table 1. The PUFA/SFA-C18:0 and MUFA+PUFA/SFA-C18:0 ratios showed no differences between the products (p > 0.05), and they were similar to those reported by other researchers (Romero et al., 2013; Rubio et al., 2008).

Decreased PUFA/SFA-C18:0 and MUFA+PUFA/SFA-C18:0 ratios were observed for all study samples after 180 days of storage probably as the results of % SFA of total fatty acids increase and % PUFA and MUFA of total fatty acids decrease. The sea salt sample (S) had the highest PUFA/SFA-C18:0 and MUFA+PUFA/SFA-C18:0 ratios after ripening and during storage, showing that it had a better nutritional composition as compared with other samples.

After 180 days of chilling storage total SFA content in the sausages was on average 37 – 43 % and the differences between all of the groups were not significant. The most prevalent SFAs were palmitic (16:0) (~ 25 % of total fatty acids) and stearic (18:0) (~ 13 %) acids. SFAs have generally been labeled as the culprits for cancer and coronary heart disease, although C18:0 is considered as a neutral fatty acid (Mahan and Escott-Stump, 2000). The content of C16:0 fatty acids of treatment S was lower (p ≤ 0.05) than treatment AW, P and control after storing.

The stable SFA and MUFA amount and the minor decrease in PUFA amount during storage suggested that the oxidation process in the sample with sea salt (S) was lower compared to other samples. The overall mean level of MUFAs was 52 % of total fatty acids, which did not differ between the four groups of sausage samples after 180 days of storage. PUFA content in the sausages was on average 12 % of total fatty acids after ripening, and was influenced (p ≤ 0.05) by the time of sausage storing but not by the additives.

PUFA decreased (p ≤ 0.05) in all samples after 180 days of storage by around 5.62 % for C, 4.53 % for S, 6.83 % for AW, and 6.07 % for P sample respectively. It was also noticed, that n-6 fatty acids decreased in all samples at the end of the storage period by around 4.98 % for C, 4.26 % for S, 6.20 % for AW, and 5.61 % for the P sample respectively. PUFA(s) reduction was due to oxidative and hydrolytic reactions that occurred during storage (Yi-Chen et al., 2008; Rubio et al., 2008; Valencia et al., 2006). Long hydrocarbon chains and high unsaturation of PUFA made them more susceptible to oxidation and hydrolytic reactions than the SFA (Yi-Chen et al., 2008).

PUFA(s) are protective against cardiovascular diseases and they lower total cholesterol (Ander et al., 2003). The PUFA/SFA (P/S) ratio could be a good indicator of the nutritional quality of sausage. The British Department of Health (1990) recommended that the P/S ratio should be above 0.45. In our study the P/S ratios after maturation were a little below 0.45; however, after 180 days of chilling storage the P/S ratios were below 0.2 in all study samples which indicated the important loss of PUFAs, which is significant for human health. The decline of P/S ratio during storage could be caused by loss of PUFAs and increased SFAs.

There was a number of long-chain n-3 PUFAs in the sausages. The overall mean n-3 fatty-acid content in the sausages was between 1.02 and 1.28 and it was not influenced by the additions but by the storing time of the products. Decreases (p ≤ 0.05) in n-3 fatty acids in two samples of the control (~ 0.42 %) with probiotic strains (~ 0.57 %) were observed after chilling storage. The n-6/n-3 ratio should be approximately 1-4/1 because excessively high n-6/n-3 ratio increases cardiovascular risk (Simopoulos, 2002). The results showed an n-6/n-3 ratio between 9.69 and 16.90 after the ripening process and between 8.92 and 9.45 after 180 days of storage. These results could be explained by the decrease (p < 0.05) in n-6 fatty acids in all samples at the end of the storage period by oxidation of linoleic acid (C18:2, n-6).

All sausages produced like the rest of the Western fermented sausages had an excessively high ratio, which was around 10-11/1 due to the extremely low content of n-3 fatty acids (EPA, DHA) (Valencia et al., 2006); thus, they contributed little in terms of dietary recommendations (Romero et al., 2013). Increases in SFA were observed (p ≤ 0.05) in all samples (~ 10 %), except in the sample with sea salt only (S), in which SFA was constant for all the storage period. In general, while the SFA and MUFA amounts of fermented sausage samples increased during storage, PUFA amounts decreased. The increase in the amounts of SFA and MUFA and decrease in PUFA could be attributable to the oxidation of PUFA during storage.

Analysis of conjugated dienes and TBARS values

Lipid peroxidation is a major cause of quality deterioration in meat products. Frankel (1998) reported that the formation of conjugated double bonds (C=C-C=C), which is related to the production of hydroperoxides, occurs in the early stage of lipid oxidation. Two treatments with probiotic strains (P) and with sea salt (S) had lower concentrations of conjugated dienes (p ≤ 0.05) compared to the control sample (C) after ripening (Table 2). In all treatments, the concentration of conjugated dienes was stable during chilling storage. An exception was a probe with probiotic mixture for which the count of primary oxidation products increased (p ≤ 0.05) during storage.

Table 2 – Influence of acid whey and probiotic strains addition and storage time on conjugated diene, TBARS values (means ± standard deviation). 

  Storage time (day)
0 90 180
Conjugated diene (µmol mg–1) C 0.25 ± 0.03a 0.24 ± 0.02ab 0.23 ± 0.02a
S 0.17 ± 0.01bA 0.22 ± 0.01aB 0.26 ± 0.05a
AW 0.24 ± 0.04a 0.26 ± 0.01b 0.27 ± 0.01a
P 0.19 ± 0.02b 0.24 ± 0.02ab 0.75 ± 0.01b

TBARS values (mg MDA kg–1) C 1.88 ± 0.19aA 1.40 ± 0.28aB 1.45 ± 0.18a
S 2.80 ± 0.57b 1.97 ± 0.40ab 2.46 ± 0.28b
AW 2.96 ± 0.14bA 2.46 ± 0.23bB 2.70 ± 0.29b
P 2.66 ± 0.21bA 2.48 ± 0.17bB 2.43 ± 0.20bC

Means followed by the same letters within columns (lower case letters) and row (capital letters) are not different (p < 0.05). C - control with curing mixture and water, S - organic sample with sea salt and water, AW - organic sample with sea salt and acid whey added, P - organic sample with sea salt and starter cultures of probiotic strain added.

A higher concentration (p ≤ 0.05) of conjugated dienes was observed in sausage with probiotics mixture compared to other samples at the end of the storage period (~ 0.75 µmol mg–1). It is likely that hydrogen peroxide produced by probiotic strains could be the source of free radicals which attacked unsaturated fatty acids and transformed them into the conjugated diene forms (Lobo et al., 2010).

The 2-thiobarbituric acid-reactive substances (TBARS) value is most widely used to determine the later stage of lipid oxidation in meat products. TBARS values are measured mainly for aldehydes, carbonyls and hydrocarbons, which contribute to off-aromas and flavor in meat products (Igene, 1985). Verma and Sahoo (2000) indicate concentrations of secondary lipid oxidation products between 1.0 and 2.0 mg kg–1 as threshold values for rancidity. However, according to Chouliara et al. (2008), the TBARS values of 3.0 mg kg–1 are associated with not only rancidity odor and flavor. Accordingly, only the sample with curing agent in the present study did not exceed the threshold values until the end of the storage period.

The organic sausages (S, AW, P) did not exceed the value of 3.0 mg kg–1 which is the indicator of oxidative rancidity of meat products. Incorporating acid whey and probiotic strains in the composition of experimental model fermented sausage had no effect (p ≤ 0.05) on TBARS values at 0, 90 and 180 days of storage as compared with the organic sample with sea salt (S) (Table 2). However, higher TBARS values (p ≤ 0.05) were observed throughout the chilling storage period in all organic samples (S, AW, P) compared to control (with curing salt) probably because S, AW and P had no antioxidant effect (p ≤ 0.05) compared to the curing salt treatment (C).

Storage time had significant effects on TBARS measurements in the case of control and two experimental samples with acid whey (AW) and probiotic strains (P). A decrease in TBARS values (p ≤ 0.05) was observed in C, AW and P samples after 180 days of storage. Low TBARS values at the end of storage could also be a result of the reaction of MDA with amino acids, sugar and other compounds in complex (Kolakowska and Deutry, 1983). The highest TBARS values were observed in samples with acid whey after ripening (2.96 ± 0.14) and at the end of the storage period (2.70 ± 0.29).

Some authors have pointed out that the presence of other compounds (e.g. amide groups, other aldehydes from smoking process) produced in meat from enzyme proteolysis during ripening could increase the TBARS values (Kolakowska and Deutry, 1983) after ripening (0 day). As expected the uncured sausage samples resulted in the most lipid oxidation. The cured - control (C) had the lowest numerical TBARS value (1.40-1.88 mg MDA kg–1). These results were expressed because nitrite or nitric oxide (derived from nitrite) are considered effective against lipid oxidation in traditionally cured meat products. In general, this additive can chelate iron from heme or can act with lipid peroxyl radicals to form non-radical addition products which cause a break in the radical chain processes of lipid oxidation. Ghiretti et al. (1997) found similar TBARS values during the long-term storage (5 months) of fermented sausage containing a number of antioxidants. Zanardi et al. (2002) found for vacuum-packed sausages TBARS values between 0.40 and 3.90 mg MDA kg–1.

Color parameter

Oxidative processes in meat and meat products lead to the degradation of lipids and proteins (including pigments) which in turn contribute to the deterioration in color, texture (cross linking binding) and flavor (Zanardi et al., 2002). Lipid oxidation products could interact with heme pigment, resulting in increased susceptibility of heme iron to oxidation and meat product deterioration (Alderon et al., 2003).

All uncured-sausages packed in vacuum maintained good color quality throughout the chilling storage period (Table 3). After the ripening process no differences were found in terms of lightness (L*) between sausage samples. After 90 days of chilling storage, a lower L* value (p ≤ 0.05), around 10, was observed for sample with see salt (S) compared to other samples.

Table 3 – Influence of acid whey and probiotic strains addition and storage time on color parameters (means ± standard deviation). 

  Storage time (day)
0 90 180
L* C 58.66 ± 3.29 58.31 ± 5.44 54.5 ± 3.3a
S 57.75 ± 5.05 47.02 ± 9.03a 57.11 ± 4ab
AW 59.2 ± 5.5 57.04 ± 3.49 58.29 ± 2.84b
P 60.47 ± 2.9 61.49 ± 3.33b 62.13 ± 2.63c

a* C 8.55 ± 1.94a 8.95 ± 2.2a 10.23 ± 1.24a
S 6.34 ± 1.49bA 8.84 ± 2.76aB 8.12 ± 2.13bAB
AW 9.18 ± 1.68a 9.06 ± 3.49a 9.87 ± 1.09a
P 6.72 ± 0.84b 6.53 ± 1.17b 6.58 ± 0.84cb

b* C 7.39 ± 0.79aA 8.87 ± 1.05B 8.03 ± 0.84AB
S 6.71 ± 0.69 7.33 ± 2.68 7.84 ± 0.99
AW 7.06 ± 0.72A 8.18 ± 0.9B 8.49 ± 0.74B
P 6.41 ± 0.59bA 7.3 ± 0.64B 7.85 ± 0.4B

Means followed by the same letters within columns (lower case letters) and row (capital letters) are not different (p < 0.05). C - control with curing mixture and water, S - organic sample with sea salt and water, AW - organic sample with sea salt and acid whey added, P - organic sample with sea salt and starter cultures of probiotic strain added.

At the end of the storage period differences (p ≤ 0.05) were found between samples. The highest L* value was observed for the AW sample and the lowest for control (C). The higher (p ≤ 0.05) lightness value (L*) of probes with acid whey (AW) could be due to the fermentation process which created acid compounds and increased lightness of sausage samples. Sammel and Claus (2003) proved that whey protein concentrates increased lightness compared to the control sausage. There were no differences in lightness of sausage samples from the beginning of the storage period until the end of the process (180 days).

There was no difference (p ≤ 0.05) in redness between uncured samples with acid whey (AW) and the cured-control sample during all the storage period. Redness (a*) which has been found as an indicator of color stability in meat products during storage remained constant (p > 0.05) throughout storage. A slight decrease was observed during the 180 days of storage in the sample with probiotics (P) probably due to the oxidation of lipids (the highest TBARS and conjugated dienes content) initiated heme pigments, oxidation and decreases in the red color of sausages (Carlsen et al., 2005). There is a reason to believe that the mixture of probiotic bacteria could demonstrate the antagonistic effect on the closely related kind and produced the hydrogen peroxide for protection. The hydrogen peroxide produced by probiotic strains can react with myoglobin (Fe2+) to ferrylmyoglobin (Fe4+), which acts as a pro-oxidant on lipids (Carlsen et al., 2005).

The redness (a*) of the organic sausage with acid whey in a slice just after ripening was assessed as high as was the sample with curing mixture addition. During storage the most intensive red coloring was observed for control sample (10.23) and for organic sample with acid whey addition (9.87). Other authors have observed an increasing pinking trend when the whey protein was added (Sammel and Claus, 2003) to the meat.

Yellowness (b*) values increased (p ≤ 0.05) from 7.38 to 8.50 during the 180 days of storage. Sausage color turned yellow rather than blue. Also Rubio et al. (2008) and García-Esteban (2004) found an increase in yellowness probably due to rancidity during long-term storage of fermented meat products.

More research should be done to verify whether the addition of antioxidants in combination with acid whey and probiotic bacteria improve the oxidative stability of uncured meat product.

Conclusions

At the end of storage period, the salted sausages had the same content of PUFA as compared to cured samples. However, the results of CD and TBARS measurements suggested that the addition of acid whey and mixture of probiotic strains to nitrite-free sausage formulation were barely able to protect lipids against oxidation in comparison to nitrite during vacuum storage.

The use of acid whey influenced the desired red-pinkish color of organic fermented sausage after ripening and after 180 days of storage period.

Acknowledgements

The research presented in this paper was supported by a Ministry of Agriculture and Rural Development grant, number PKre-029-4-3/12(608).

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Received: March 18, 2014; Accepted: August 20, 2014

*Corresponding author karolina.wojciak@up.lublin.pl

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