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Journal of Venomous Animals and Toxins

Print version ISSN 0104-7930On-line version ISSN 1678-4936

J. Venom. Anim. Toxins vol.7 no.1 Botucatu  2001 

THESIS. I. A. Thomazini-Santos submitted this for her Doctorate in Tropical Biology publicly examined at the Department of Tropical Diseases and Diagnostic Imaging, Botucatu School of Medicine, UNESP, Botucatu, Brazil, 2000.

Advisor. Prof. Dr. Benedito Barraviera



ABSTRACT. The objective of this study was to investigate the effect of the following agents on the fibrinolytic process: e-aminocaproic acid, tranexamic acid, and aprotinin. These substances were added to fibrin adhesive composed of the thrombin-like fraction from snake venom and bubaline fibrinogen. This adhesive was applied to surgical incisions on the skin of rats to appose wound edges. The animals were observed up to the 7th day postoperative. The control in this experiment was the rat itself, in which three suture stitches were made on the opposite side to that of the adhesive. The efficient coaptation of surgical wounds was assessed by macroscopic observation for the presence of dehiscence, determination of skin tensile strength, and histopathological examination of the surgical scar. The effect of the antifibrinolytic agents was also evaluated in vitro by observing euglobulin lysis time. The results obtained allowed the following conclusions:

- The macroscopic examination of wound edge coaptation both in the experimental and controls groups did not show any statistically significant difference;

- With regard to tensile strength evaluation, it should be emphasized that although statistically significant differences were not observed between the experimental and controls groups, the values obtained were higher in the fibrin adhesive evaluation on the 7th day postoperative both for maximum strength and for deformation of maximum strength;

- Analysis of the histopathological characteristics of the different groups revealed that experimental groups 3 and 4 (fibrin adhesive + e-aminocaproic acid and fibrin adhesive + aprotinin) respectively showed adverse reactions during coaptation of surgical incision edges;

- All the antifibrinolytic agents used in this study prolonged euglobulin lysis time in vitro when compared to the adhesive alone;

- The snake venom-derived fibrin adhesive does not require these studied antifibrinolytic agents for successful coaptation of skin surgical edges.

KEY WORDS: Fibrin adhesive, e-aminocaproic acid, tranexamic acid, aprotinin, fibrinogen, bubaline, snake venom, euglobulin lysis time.



n1env1.gif (159 bytes) CORRESPONDENCE TO:
I. A. THOMAZINI-SANTOS - Divisão de Hemocentro, Laboratório de Hemostasia, Faculdade de Medicina de Botucatu, Universidade Estadual Paulista - UNESP, Distrito de Rubião Junior, s/n, CEP 18618-000, Botucatu, São Paulo, Brasil.

Phone/Fax: 55 14 6802 6041 and 6802 6234


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