S100 negative granular cell tumor of the oral cavity: dermoscopy and surgical approach^*

Abstract:

We report the case of a 47-year-old male patient with S100 negative granular cell tumor of the oral cavity, focusing on dermoscopic features as well as surgical approach, not previously reported in the literature. The study contributes to the literature on dermoscopy and surgical treatment for this tumor and provides a practical approach to differentiating non-neural granular cell tumors and granular cell tumors.

Keywords:
Dermoscopy; Granular cell tumor; Surgery, oral

INTRODUCTION

Granular cell tumors (GCT), also known as Abrikossoff tumors, are uncommon benign neoplasms probably derived from Schwann cells. They are usually located in the oral mucosa, specifically the tongue.¹ Non-neural granular cell tumor (NNGCT) is a rare and recently described tumor. Only five cases have been reported with involvement of the oral mucosa, with no reports of dermoscopy or surgical treatment. The current case report contributes to the literature on dermoscopy and surgical management of this tumor and provides a practical approach to differentiating between non-neural granular cell tumors and granular cell tumors.

CASE REPORT

A 47-year-old male patient presented with a 5-month history of a rapidly growing solitary lesion on the mucosa and semimucosa of the lower lip. Physical examination showed a painful solid exophytic bleeding ulcerated pink tumor measuring 12x13mm, with telangiectasias and yellowish hue in the center involving the mucosa, semimucosa, and linea alba of the left third of the lower lip (Figure 1A). Dermoscopy showed a structureless heterogeneous yellowish and white central zone and an apple jelly hue in the lower lateral portion of the tumor with multiple polymorphous vessels in the periphery (dotted, coiled, and looped) (Figure 1B). Histological examination revealed an exophytic ulcerated lesion consisting of cells with granular cytoplasm and homogeneous nuclei with low mitotic rate and no cellular atypia, located on the lamina propria and with skeletal muscle infiltration, without lymphatic involvement. (Figure 2A). On immunohistochemistry, the lesion was negative for S100, CD57, EMA, CD34, and neuron-specific enolase and positive for vimentin, CD68, and calretinin, weakly positive for CD56, with Ki67 proliferation index of approximately 3% (Figure 2B and 2C).

Figure 1
A Exophytic tumor on the lower lip. B - Dermoscopy: structureless white yellowish zone (red arrow) and polymorphous vessels (black arrows)

Figure 2
A Histology showing granular cell tumor and infiltration of skeletal muscle on different magnifications (Hematoxylin & eosin, x4, x10, and x40). B - Immunohistochemistry showing negative S100 staining C - Tumor cells positive for CD56, CD68 e vimentin

Based on these findings, a diagnosis of non-neural granular cell tumor was made. Further imaging exams were performed, finding no visceral involvement of the tumor. The surgical approach consisted of a full-thickness V-shaped excision with a 5mm margin under tumescent anesthesia. A 3-layer closure (oral mucosa, orbicularis oris muscle, and overlying skin) was performed, and peripheral and deep margins were clear (Figure 3). After one year of follow-up, no evidence of recurrence or metastasis was seen.

Figure 3
Surgical approach. A Presurgical demarcation with 5mm margin. B - Fullthickness V-shaped excision. C - Three-layer closure (oral mucosa, orbicularis oris muscle, and skin)

DISCUSSION

NNGCT or S100 negative granular cell tumor is one of the tumors of the oral cavity histologically composed of granular cells.² Histologically, GCT and NNGCT are indistinguishable, and they are usually differentiated by immunohistochemistry.³ However, there are some clinical, histological, and immunohistochemical differences that may help distinguish between these unusual tumors (Table 1).^2,4 As recently described, the NNGCT IHC profile is not well defined; however, vimentin and CD68 positivity is well established. 3 In the literature, the only five cases of this tumor in the oral cavity report surgical excision as the treatment of choice, but there are no details on margins or any suggested surgical approach.^2,3,5,6 Full-thickness V excision with 5mm margin and primary closure is a feasible approach with excellent functional and cosmetic results as observed in our patient.

Table 1
Differences between granular cell tumor and non-neural granular cell tumor

Characteristic	GCT	NN-GCT
Clinical examination
Location	Head and Neck	Entire body
Oral cavity	Common	Uncommon
Number	Solitary	Solitary
Histology
Architecture	Polypoid	Polypoid
Level of involvement	Dermis, adipose tissue, muscle	Papillary dermis
Pseudoepithelioma­tous hyperplasia	+++	+
Delimitation	+/-	+
Cytologic atypia	+/-	+/++
Mitotic activity	-	++
Pustulo-ovoid bodies of Milian	+	?
IHC reactivity
S-100	+	-
Vimentin	+	+
CD68	- (71%)	+
Calretinin	+	-*

• ^*
  however positive in the present case

NNGCT dermoscopy had never been reported before. Polymorphous vascular structures in the periphery such as dotted, coiled, and looped vessels and structureless yellowish zone were some of the findings observed in the tumor dermoscopy, similar to those in cutaneous GCT.⁷ Additional dermoscopy reports are needed to better characterize this type of tumors and possibly define dermoscopic characteristics that could guide the clinician to an accurate clinical diagnosis.

• *
  Work conducted at the Department of Dermatology, Hospital Militar Central, Universidad Militar Nueva Granada, Bogotá, Colombia.
• Financial support: None.

REFERENCES

• ¹
  Plantier F. Granular cell tumour or Abrikissoff's tumour. Ann Dermatol Venereol. 2013;140:399-402.
• ²
  Solomon LW, Velez I. S-100 Negative Granular Cell Tumor of the Oral Cavity. Head Neck Pathol. 2016;10:367-73.
• ³
  Basile JR, Woo SB. Polypoid S-100-negative granular cell tumor of the oral cavity: a case report and review of literature. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003;96:70-6.
• ⁴
  Torrijos-Aguilar A, Alegre-de Miquel V, Pitarch-Bort G, Mercader-García P, Fortea-Baixauli JM. Cutaneous granular cell tumor: a clinical and pathologic analysis of 34 cases. Actas Dermosifiliogr. 2009;100:126-32.
• ⁵
  Lerman M, Freedman PD. Nonneural granular cell tumor of the oral cavity: a case report and review of the literature. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007;103:382-4.
• ⁶
  Rawal YB, Dodson TB. S-100 Negative Granular Cell Tumor (So-called Primitive Polypoid Non-neural Granular Cell Tumor) of the Oral Cavity. Head Neck Pathol. 2017;11:404-12.
• ⁷
  Popadic M. Dermoscopy of cutaneous Abrikossoff tumor (granular cell tumor) in a pediatric patient. J Am Acad Dermatol. 2015;73:e137-8.

Publication Dates

• Publication in this collection
  Jan-Feb 2019

History

• Received
  27 July 2017
• Accepted
  21 Feb 2018