Note on the effect of high nitrate concentration and light intensity on the growth and uptake rates of Phaeodactylum tricornutum (Bohlin) culture

ARTIGOS

Note on the effect of high nitrate concentration and light intensity on the growth and uptake rates of Phaeodactylum tricornutum (Bohlin) culture

Gilda Schmidt

Instituto Oceanógrafico da Universidade de São Paulo

SYNOPSIS

The effect of high nitrate concentration and light intensity on chlorophyll-a synthesis, cell number and nitrate assimilation on P. tJt¿coh,nuutum culture, was determined. Growth and uptake rates were determined as a function of nitrate concentration ranging from 0.40 to 35.40 µg/l. The Ks showed high values, when compared with those obtained with lower nitrate concentration. The percentual variation of Ks was greater than that of Vmax.

Descriptors: Nitrates, Chlorophylls, Phaeodactylum tricornutum, Growth, Culture media, Nutrients (mineral), Light intensity, Cell.

RESUMO

Foi determinado o efeito dc ama alta concentração de nitrato e alta incensidade luminosa na síntese de clorofila-α, número de células e assimilação nitrato em uma cultura de P. triconotum. As velocidade de crescimento e de assimilação foram determinados em função de concentrações de nitrato variando de 0,40 a 35,40 µg-at/l e intensidade luminosa de 12 KLUX. A Ks e a Vmax apresentaram valores altos, comparativamente com valores obtidos em concentrações mais baixas de nitrato e menor intensidade luminosa. A variação porcentual da Ks foi maior que a variação porcentual da Vmax.

Descritores: Nitratos, Clorofilas, Phaedactylum tricornutum, Crescimento, Meio de cultura, Nutrientes minerais, Intensidade luminosa, Célula.

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(Received 10-Dec-1983; accepted 16-May-1983)

• BEARDALL, J. & MORRIS, I. 1976, The concept of ligth intensity adaptation in marine phytoplankton: some experiments with Phaedactylum tricornutus. Mar. Biol., 37:377-387.
• COLLOS, Y. 1980. Transient situations in nitrate assimilation by marine diatoms. 1. Changes in uptake parameters during nitrogen starvation. Limnol. Oceanogr., 25(6):1075-1081.
• _____ 1982. Transient situations in nitrate assimilation by marine diatoms. 2. Changes in nitrate and nitrite following a nitrate pertur bation. Limnol. Oceanogr., 27(3): 528-535.
• _____ & SLAWYK, G. 1979. ¹³C and ¹⁵N uptake by marine phytoplankton. I. Influence of nitrogen source and concentration in laboratory culture of diatoms. J. Phycol., 15:186-190.
• DUGDALE, E. 1967. Nutrient limitation in the sea:dynamics, identification and significance. Limnol. Oceanogr., 12:685-695.
• EPPLEY, R. W. & THOMAS, W. H. 1969. Comparison of half-saturation constants for growth and nitrate uptake of marine phytoplankton. J. Phycol., 5(4):375-379
• FAIRCHILD, E. & SHERIDAN. R. P. 1974. A physiological investigation of the hot spring diatom, Achanates exigua Grün. J. Phycol., 10:1-4.
• GUILLARD, R. R. L. & RYTHER, J. H. 1962. Studies of marine planktonic diatoms. I. Cyclotella nana (Hustedt) and Detonula confervacea (Cleve). Gram. Can. J. Microbiol., 8:229-239.
• MacISAAC, J. J. & DUGDALE, R. C. 1969. The kinetics of nitrate and ammonia uptake by natural population of marine phytoplankton. Deep-sea Res., 16:45-57.
• MEEKS, J. C. 1974. Chlorophylls. In: Stewart, W. D. P., ed. - Algal physiology and biochemistry. London.
• MORRIS, I.; GLOVER, H. E. & YENTSCH, C. S. 1974. Products of photosynthesis by marine phytoplankton: the effect of environmental factors on the relative rates of protein synthesis. Mar. Biol., 27:1-9.
• MULLIN, J. B. & RILEY, J. P. 1955. The spectrophotometric determination of nitrate in natural waters, with particular reference to sea-water. Analytica chem. Acta, 12:64-90.
• PAASCHE, E. 1968. Marine plankton algae with light-dark cycles. II. Dytilum brigthwellii and Nitzschia turgidula Psicologia Pl., 21:66-77.
• SCHMIDT, G. 1982. Algumas observações sobre a cinêtica do fitoplãncton marinho. 1. Influencia da concentração de nitrato e amonia na velocidade de crescimento e de assimilação desses nutrientes na diatomácea Phaedactylum tricornutum (Bohlin) . Bolm Inst, oceanogr., S Paulo, 31(2):13-27.
• SOURNIA, A. 1974. Circadian periodicities in natural populations of marine phytoplankton. Adv. mar. Biol., 12:325-389.
• STRICKLAND, J. D. H. & PARSONS, T. R. 1968. A practical handbook of seawater analysis. Bull. Fish. Res. Bd Can., (167):1-311.
• TEIXEIRA, C. & VIEIRA, A. A. H. 1976. Nutrient experiment using Phaeodactylum tricornutum as an assay organism. Bolm Inst, oceanogr., S Paulo, 25:29-42.
• THOMAS, H. W. 19 70. Effect of ammonium and nitrate concentration on chlorophyll increases in natural tropical Pacific phytoplankton populations. Limnol. Oceanogr., 15:386-394.
• ______& DODSON, A. N. 1974. Effect of interactions between temperature and nitrate supply on the cell-division rates of two marine phytoflagellates. Mar. Biol., 24:213-217.
• VIEIRA, A. A. H. 1975. Estudos experimentais em fitoplâncton marinho. Dissertação de mestrado. Universidade de São Paulo, Instituto Oceanográfico, 115p.

Publication Dates

• Publication in this collection
  06 June 2012
• Date of issue
  1983

History

• Accepted
  16 May 1983
• Received
  10 Dec 1983