SciELO - Scientific Electronic Library Online

vol.19 issue1Severe infective endocarditis with systemic embolism due to community associated methicillin-resistant Staphylococcus aureusST630Sclerosing encapsulating peritonitis in HIV-infected patient on dialysis author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Brazilian Journal of Infectious Diseases

Print version ISSN 1413-8670

Braz J Infect Dis vol.19 no.1 Salvador Jan./Feb. 2015 

Case Reports

Disseminated Fusarium infection in autologous stem cell transplant recipient

Vivian Iida Avelino-Silva a   b   *  

Jessica Fernandes Ramos a   b  

Fabio Eudes Leal a   b   c  

Leonardo Testagrossa d  

Yana Sarkis Novis b  

aDepartment of Infectious and Parasitic Diseases, University of Sao Paulo Medical School, São Paulo, SP, Brazil

bHospital Sirio-Libanês, Sao Paulo, SP, Brazil

cDivision of Clinic Immunology and Allergy, Medical School, University of São Paulo, São Paulo, SP, Brazil

dFleury Medicina Diagnóstica, Sao Paulo, SP, Brazil


Disseminated infection by Fusarium is a rare, frequently lethal condition in severely immunocompromised patients, including bone marrow transplant recipients. However, autologous bone marrow transplant recipients are not expected to be at high risk to develop fusariosis. We report a rare case of lethal disseminated Fusarium infection in an autologous bone marrow transplant recipient during pre-engraftment phase.

Key words: Stem cell transplant; Autologous; Fusarium

A 53-year-old male patient, diagnosed with a grade II follicular lymphoma, was submitted to an autologous bone marrow transplant (BMT) as therapeutic procedure follow- ing recurrence to initial chemotherapy. He had been treated with eight cycles of R-CVP (Rituximab, Cyclophosphamide, Vincristine and prednisone), followed by three cycles of RICE (Rituximab, Iphosphamide Carboplatin and Etoposide) and two cycles of R-DA-EPOCH (dose-adjusted etoposide, prednisone, vincristine, cyclophosphamide, doxorubicin plus rituximab). Conditioning regimen was prescribed with BEAM (carmustine, etoposide, cytosine arabinoside, melphalan). Prophylactic antimicrobial treatment was prescribed with fluconazole 200 mg QD. He started with neutropenia in the D + 1 post-transplant, and presented fever in the D + 3 post-transplant, followed by intense myalgia and papular skin lesion with rapid dissemination. Some of the papular lesions progressed with central ischemia and necrosis (Fig. 1). Empiric antimicrobial treatment with meropenen, vancomycin, and liposomal amphotericin 5 mg/kg QD was initiated. Baseline total leukocyte count at the onset of the infectious episode was 20 leucocytes. A skin biopsy revealed abundant hyphae structures (Fig. 2). Concomitantly, blood cultures identified Fusarium solani through macroand microculture. The patient was immediately transferred to the intensive care unit, and voriconazole 400 mg IV BID was associated to the antifungal therapy. Ophthalmologic evaluation revealed no signs of endophthalmitis, and radiographic images were unremarkable. Laboratory findings were heavy myoglobinuria, with normal creatine phosphokinase, worsened renal function with increased blood lactate and elevation of both C-reactive protein and pro-calcitonin. Serum (1-3)-β-n-glucan was not assessed. Granulocyte was transfused in two consecutive days without significant improvement of the clinical condition. The patient developed refractory septic shock and, in spite of intensive care support, renal replacement therapy, and mechanical ventilation, progressed to unresponsive cardiac arrest four days after initial clinical presentation. The patient had no previous history of fungal diseases. No skin or nail lesion had been observed at thorough daily physical examination prior to the terminal event. Fungal susceptibility testing performed by E-test method revealed resistance to fluconazole and susceptibility to voriconazole and amphotericin. Investigation regarding potential source of infection was conducted, and cultures of tap and shower bath water obtained from the patient's hospital unit were negative for fungal growth.

Fig. 1 -  Skin papular lesions (A) progressed with central ischemia and necrosis (B). 

Fig. 2 -  Skin biopsy showing abundant septate hyphae structures. (A) 200× hematoxylin-eosin stain revealing basophilic yeast and hyphae structures in superficial dermis and perivascular sites, with minimal inflammatory component. (B) Skin biopsy in 100× Periodic acid-Schiff stain, revealing fungal cell walls in magenta. (C) 200× Grocott's methenamine silver stain, revealing intravascular and epidermal fungal structures, stained in black. 


Fusarium infection is a rare event following autologous BMT, probably due to less intense immunossupression relatively to allogeneic or cord cell transplant. To our knowledge, only one case report has described fusariosis in an autologous BMT recipient who survived after administration of amphotericin B, eye enucleation due to fungal endophthalmitis, and neutrophil recovery.1Other 10 reports of fusariosis in an autologous BMT described fatal outcomes.2 , 3

Our patient developed a fatal, disseminated fusariosis after a very short period of neutropenia. This unexpected outcome propelled immediate investigation of the potential source of fungal contamination. Hospital environmental contamination by fungal specimens has been demonstrated in medical literature, even in high-efficiency particulate air (HEPA) filtration-equipped units.4 , 5 Although cultures of tap and shower bath water turned out negative for fungal growth in our case, it is likely that environmental sources, either hospital-related or not, might be accounted as the infection source.

A throughout physical examination, including skin and nail inspection did not reveal any lesion that could raise suspicion for a cutaneous portal of entry, either at hospital admission or at the post-transplant period. A strong association between invasive Fusarium infection and a superficial skin lesion, especially onychomycosis or intertrigo, has been demonstrated in recent studies.6 , 7 Moreover, the presence of a Fusarium skin lesion at admission was associated with the subsequent development of invasive fusariosis in a prospective cohort.6

Our patient was not under prophylactic therapy with voriconazole, in accordance with his short period of neutropenia and low risk of invasive mold infection. However, an increasing incidence of invasive mold infections has been reported among patients with hematological malignancies,8 , 9 including autologous BMT recipients.10 In Brazil, cases of invasive fusariosis affecting patients with hematologic malignancies and hematopoietic cell transplant recipients have been reported to increase over the years 2000-2010.7 The widespread use of monoclonal antibodies, including Rituximab, might potentially increase risk for infections among hematologic patients,11 but the association with invasive molds needs further investigation.

Treatment of Fusarium infection is a challenging issue, particularly in the immunocompromised host. Lipid formulations of amphotericin B and voriconazole are the most frequently used medications for first-line monotherapy.12 Fusarium susceptibility to voriconazole is variable,13 and a breakthrough infection has been reported in 16 out of 44 consecutive fusariosis cases in a large retrospective report. Among those patients diagnosed with fusariosis, 69% were receiving prophylaxis with an extended spectrum triazole, either voriconazole (8/16; 50%) or posaconazole (3/16; 19%).14

Combination therapy with amphotericin formulations, voriconazole, itraconazole, and echinocandins have been previously reported,12 , 15 , 16 but more studies are required to explore the real benefit of this approach.

Granulocyte transfusion is often considered as a supporting therapy for patients with prolonged neutropenia or abnormal leukocyte function and severe infection. Although its efficacy is not well established, some authors suggest a beneficial effect, based on uncontrolled case reports and case series.17 , 18

In conclusion, our report illustrates a rare case of a lethal, disseminated Fusarium infection in an autologous BMT recipient during pre-engraftment phase. This unexpected manifestation reinforces the need for careful clinical evaluation of BMT candidates, as well as exhaustive vigilance of environmental cleansing procedures. Antifungal prophylaxis must be selected according to the characteristics of each patient, and further studies assessing the use of antifungal prophylaxis with activity against molds are necessary.


We acknowledge Dr. Mariana Serpa and Dr. Esper Georges Kallás for the manuscript revision and useful contribution.


Robertson MJ, Socinski MA, Soiffer RJ, et al. Successful treatment of disseminated Fusarium infection after autologous bone marrow transplantation for acute myeloid leukemia. Bone Marrow Transplant. 1991;8:143-5. [ Links ]

Boutati EI, Anaissie EJ. Fusarium, a significant emerging pathogen in patients with hematologic malignancy: ten years' experience at a cancer center and implications for management. Blood. 1997;90:999-1008. [ Links ]

Nucci M, Marr KA, Queiroz-Telles F, et al. Fusarium infection in hematopoietic stem cell transplant recipients. Clin Infect Dis. 2004;38:1237-42. [ Links ]

Teixeira AB, Silva M, Lyra L, et al. Antifungal susceptibility and pathogenic potential of environmental isolated filamentous fungi compared with colonizing agents in immunocompromised patients. Mycopathologia. 2005;160:129-35. [ Links ]

Hao ZF, Ao JH, Hao F, Yang RY, Zhu H, Zhang J. Environment surveillance of filamentous fungi in two tertiary care hospitals in China. Chin Med J (Engl). 2011;124:1970-5. [ Links ]

Varon AG, Nouer SA, Barreiros G, et al. Superficial skin lesions positive for Fusarium are associated with subsequent development of invasive fusariosis. J Infect. 2014;68:85-9. [ Links ]

Nucci M, Varon AG, Garnica M, et al. Increased incidence of invasive fusariosis with cutaneous portal of entry, Brazil. Emerg Infect Dis. 2013;19:1567-72. [ Links ]

Auberger J, Lass-Florl C, Ulmer H, et al. Significant alterations in the epidemiology and treatment outcome of invasive fungal infections in patients with hematological malignancies. Int J Hematol. 2008;88:508-15. [ Links ]

Rubio PM, Sevilla J, Gonzalez-Vicent M, et al. Increasing incidence of invasive aspergillosis in pediatric hematology oncology patients over the last decade: a retrospective single centre study. J Pediatr Hematol Oncol. 2009;31:642-6. [ Links ]

Gil L, Kozlowska-Skrzypczak M, Mol A, Poplawski D, Styczynski J, Komarnicki M. Increased risk for invasive aspergillosis in patients with lymphoproliferative diseases after autologous hematopoietic SCT. Bone Marrow Transplant. 2009;43:121-6. [ Links ]

Lanini S, Molloy AC, Prentice AG, Ippolito G, Kibbler CC. Infections in patients taking Rituximab for hematologic malignancies: two-year cohort study. BMC Infect Dis. 2013;13:317. [ Links ]

Muhammed M, Anagnostou T, Desalermos A, et al. Fusarium infection: report of 26 cases and review of 97 cases from the literature. Medicine (Baltimore). 2013;92:305-16. [ Links ]

Debourgogne A, de Hoog S, Lozniewski A, Machouart M. Amphotericin B and voriconazole susceptibility profiles for the Fusarium solani species complex: comparison between the E-test and CLSI M38-A2 microdilution methodology. Eur J Clin Microbiol Infect Dis. 2012;31:615-8. [ Links ]

Campo M, Lewis RE, Kontoyiannis DP. Invasive fusariosis in patients with hematologic malignancies at a cancer center: 1998-2009. J Infect. 2010;60:331-7. [ Links ]

Durand-Joly I, Alfandari S, Benchikh Z, et al. Successful outcome of disseminated Fusarium infection with skin localization treated with voriconazole and amphotericin B-lipid complex in a patient with acute leukemia. J Clin Microbiol. 2003;41:4898-900. [ Links ]

Candoni A, Caira M, Cesaro S, et al. Multicentre surveillance study on feasibility, safety and efficacy of antifungal combination therapy for proven or probable invasive fungal diseases in haematological patients: the SEIFEM real-life combo study. Mycoses. 2014;57(6):342-50. [ Links ]

Mellouli F, Ksouri H, Barbouche R, et al. Successful treatment of Fusarium solani ecthyma gangrenosum in a patient affected by leukocyte adhesion deficiency type 1 with granulocytes transfusions. BMC Dermatol. 2010;10:10. [ Links ]

Cherif H, Axdorph U, Kalin M, Bjorkholm M. Clinical experience of granulocyte transfusion in the management of neutropenic patients with haematological malignancies and severe infection. Scand J Infect Dis. 2013;45(2):112-6. [ Links ]

Authorship VIAS conceived the manuscript, participated in the collection of clinical data, study design and manuscript writing; JFR and FEL participated in the collection of clinical data and manuscript revision; LT participated in the assemblage of histopathology data and manuscript writing; YSN participated in the collection of clinical data and manuscript review. All authors read and approved the final manuscript.

Received: December 26, 2013; Accepted: August 01, 2014

*Corresponding author at: Faculdade de Medicina da Universidade de São Paulo, Av. Dr. Enéas de Carvalho Aguiar, 470, Cerqueira César, 05403-000 Sao Paulo, SP, Brazil. E-mail address: (V.I. Avelino-Silva).

Conflicts of interest The authors have no conflicts of interest to declare.

Creative Commons License This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.